Article
Food Science & Technology
Shixiang Wu, Xingping Zhang, Wang Chen, Guohao Zhang, Qian Zhang, Hualin Yang, Yu Zhou
Summary: Inspired by the strong binding capacity of pyrophosphate (ppi) and copper ion (Cu2+), a ratiometric fluorescence immunoassay triggered by alkaline phosphatase (ALP) was developed for the detection of zearalenone (ZEN). This method exhibited high accuracy and reliability, with a limit of detection (LOD) of 0.014 ng/mL and a linear detection range of 3.51-17.78 ng/mL. It provides a new approach for efficient and accurate detection of ZEN.
Review
Chemistry, Analytical
Bolong Fang, Qirong Xiong, Hongwei Duan, Yonghua Xiong, Weihua Lai
Summary: Lateral flow immunoassay (LFIA) is a widely used rapid detection technology in the areas of food safety, environmental monitoring, and clinical diagnosis. The conventional LFIA based on gold nanoparticles (AuNPs) suffers from low sensitivity. In contrast, fluorescence LFIA (FLFIA) using quantum dots (QDs) as signal labels shows higher sensitivity. This review systematically summarizes the emerging strategies of tailing QDs to enhance the sensing performance of LFIA and provides insights into the future development of QDs-based LFIA.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Article
Engineering, Environmental
Lijiao Ao, Tao Liao, Liang Huang, Shan Lin, Kui Xu, Jiangtao Ma, Shaorong Qiu, Xiangyu Wang, Qiqing Zhang
Summary: This study developed a highly sensitive and portable multi-target serum cancer biomarker detection method. By using NIR-II emitting quantum dots and three-dimensional distribution assembly, the sensitivity and fluorescence signals of the test strip were successfully enhanced. With this method, simultaneous detection and quantitative analysis of CEA, Cyfra21-1, and NSE tumor biomarkers were achieved, with high sensitivity and specificity demonstrated in lung cancer patients and healthy individuals.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Chemistry, Applied
Xianfeng Lin, Changxin Li, Xinyu Tong, Nuo Duan, Zhouping Wang, Shijia Wu
Summary: A portable paper-based microfluidic aptasensor is developed for simultaneous and visual detection of ZEN and OTA, offering potential applications in food safety testing due to its advantages of easy operation and low sample consumption.
Article
Biochemical Research Methods
Jingming Zhou, Qingbao Yang, Chao Liang, Yumei Chen, Xiaoli Zhang, Zhanxiang Liu, Aiping Wang
Summary: An efficient method for the detection of OTA in corn, utilizing a quantum dots-based fluorescent immunochromatographic assay (QDs-ICA), exhibited excellent specificity and accuracy.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Applied
Zixin Jin, Wei Sheng, Junli Liu, Chenchen Liu, Yueru Ma, Shuo Wang, Wanli Zhang, Na Huang
Summary: A novel fluorescence immunoassay combining MnO2 nanoflowers loading multicolor quantum dots and gluta-thione destroying MnO2 nanoflowers to release quantum dots with magnetic separation was developed for rapid, ultra-sensitive, and simultaneous quantitative detection of four mycotoxins in cereal samples. The assay exhibited a wide linear range and low limits of detection. The simultaneous detection of four mycotoxins could be achieved within a short time. The test results were confirmed by ultra-performance liquid chromatography tandem mass spectrometry.
Article
Chemistry, Analytical
Ming Li, Chenfei Han, Chuqin Yang, Mingna Sun, Jinsheng Duan, Xuchun Qiu, Daolin Du
Summary: A ultrasensitive fluorometric oligonucleotide immunoassay (UFOIA) based on a fluorometric oligonucleotide and magnetic separation was proposed for simultaneous detection of two mycotoxins, exhibiting improved sensitivities compared to ELISA.
ANALYTICAL METHODS
(2022)
Article
Spectroscopy
Yao Xiao, Xingping Zhang, Liyuan Ma, Huajuan Fang, Hualin Yang, Yu Zhou
Summary: A simple dual-mode immunoassay for detecting Ochratoxin A (OTA) was developed using Gquadruplex/N-methylmesoporphyrin IX (G4/NMM) and 3,3',5,5'-tetramethylbenzidine (TMB). The method showed a wide linear range and good selectivity, making it suitable for practical sample detection.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Chemistry, Multidisciplinary
Wei Li Ang, Jiri Sturala, Nikolas Antonatos, Zdenek Sofer, Alessandra Bonanni
Summary: Chemically modified germanenes with different surface ligands show strong influence on intrinsic fluorescence and affect bio-conjugation ability and bio-recognition efficiency towards analyte detection. Methylated germanene material demonstrates improved calibration sensitivity, reproducibility, and linearity for real sample analysis in optical bioassays.
Article
Chemistry, Applied
Yongpeng Jin, Qian Chen, Sunlin Luo, Lidong He, Ruiqi Fan, Siwei Zhang, Chunjiang Yang, Yiqiang Chen
Summary: A novel dual near-infrared fluorescence-based lateral flow immunosensor has been developed for simultaneous detection of zearalenone and deoxynivalenol in maize, showing high accuracy and precision. This immunosensor can serve as an effective method for monitoring mycotoxins in agricultural products.
Article
Polymer Science
Jialu Shen, Zhen He, Jinwei Zhang, Hao Guo, Wei Lin, Haibin Gu
Summary: This study prepared two fluorescent carbon quantum dots (CQDs), XG-CQDs and OXG-CQDs, using xanthan gum (XG) and oxidized xanthan gum (OXG) as raw materials. The CQDs exhibited stable fluorescence in high concentration of salt and wide pH range, and showed selectivity to Cr6+ ion, making them suitable for detection and anti-counterfeiting applications. Furthermore, an OXG-PEI hydrogel was constructed, which exhibited similar photoluminescence properties as OXG-CQDs. The hydrogel could be used for Cr6+ ion detection and UV shielding, and also as a light conversion material for green LED devices.
Article
Chemistry, Multidisciplinary
Ting Meng, Zifei Wang, Ting Yuan, Xiaohong Li, Yunchao Li, Yang Zhang, Louzhen Fan
Summary: This study presents a gram-scale synthesis of red/green/blue solid-state fluorescent carbon quantum rings with high quantum yields, offering a potential alternative to REE-free phosphor-converted white LEDs. These R/G/B-SBF-CQRs-based LEDs emit warm white light with low CCT, high CRI, and high luminous efficiency, comparable to REE-phosphor-based LEDs.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Spectroscopy
Guohao Zhang, Xingping Zhang, Qian Zhang, Wang Chen, Shixiang Wu, Hualin Yang, Yu Zhou
Summary: In this study, a fluorescence immunoassay mediated by alkaline phosphatase (ALP) was established to detect zearalenone (ZEN). The fluorescence signal of oxVB1 modulated by MnO2 nanosheets (MnO2 NS) was used as the detection signal. The method achieved control over the fluorescence of oxVB1 by adjusting the concentration of ALP-antibody. Under optimized conditions, the method showed a low detection limit and high accuracy.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Chemistry, Multidisciplinary
Cheng Yang, Hao Xie
Summary: This study demonstrates the interactions between carbon dots (CDs) and biomolecules through surface modification. Various fluorescence response behaviors of modified CDs were observed, with electrostatic attraction and hydrogen bonding playing important roles in inducing aggregation and fluorescence enhancement of CDs. The study also suggests the occurrence of an inner filter effect and selective antibacterial activity of modified CDs towards Gram-positive bacteria. This work provides potential for developing CD-based techniques for detecting and visualizing proteins/bacteria, as well as selective antibacterial agents.
Article
Chemistry, Analytical
Peng Qu, Cheng-Jie Li, Jin Hu, Ge Gao, Peng Lin, Wei-Wei Zhao
Summary: Organicphotoelectrochemical transistor (OPECT) biosensing is a promising approach for biomolecular sensing. This work demonstrates the direct enzymatic biocatalytic precipitation (BCP) modulation on a flower-like Bi(2)S(3) photosensitive gate, achieving high-efficiency OPECT operation with high transconductance (g (m)). The developed OPECT aptasensor shows good analysis performance for prostate-specific antigen (PSA).
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Saranya Krishnan, Shina Sasi, Preshobha Kodakkattumannil, Salima Al Senaani, Geetha Lekshmi, Martin Kottackal, Khaled M. A. Amiri
Summary: This study aimed to develop an efficient DNA extraction protocol suitable for diverse plant species and tissues. A reliable and consistent protocol was described for the extraction of high-quality DNA from difficult-to-extract plant species. The optimized protocol was successful in extracting high-quality DNA from various plant species and tissues, making it useful for genomic studies of recalcitrant plants.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Eisaku Hokazono, Saori Fukumoto, Takeshi Uchiumi, Susumu Osawa
Summary: This study proposes a method for detecting nucleic acid amplification using pyrophosphate, which requires only two reagents and an automated analyzer. The technique has high sensitivity and reproducibility, and can detect pyrophosphate within 10 minutes. Therefore, this method has the potential to be a new, rapid, and simple detection technique for amplified nucleic acids.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Drew S. Sowersby, L. Kevin Lewis
Summary: SURE electrophoresis is a new method for concentrating samples in gels, which allows efficient detection of highly dilute DNA samples. This approach generates single bands with enhanced signal intensities and minimal band broadening.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Hongyi Liang, Guoliang Yin, Guangxi Shi, Zhiyong Liu, Xiaofei Liu, Jingwei Li
Summary: The mechanism of Echinacoside (ECH) in treating breast cancer (BC) was explored through network pharmacology and experimental validation. It was found that ECH plays an important role in anti-BC by regulating the PI3K/AKT/HIF-1 alpha/VEGF signaling pathway, and it exhibits multi-target and multi-pathway effects.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Daniel S. Kantner, Emily Megill, Anna Bostwick, Vicky Yang, Carmen Bekeova, Alexandria Van Scoyk, Erin L. Seifert, Michael W. Deininger, Nathaniel W. Snyder
Summary: This study compared the results of different methods for measuring the amount of acetyl-Coenzyme A. The colorimetric ELISA kit did not produce interpretable results, while the fluorometric enzymatic kit showed comparable results to the LC-MS-based methods depending on the matrix and extraction conditions. LC-MS/MS and LC-HRMS methods produced well-aligned results, especially when using stable isotope-labeled internal standards.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Jingyan Xu, Hanying Tan, Xionghui Ma, Linjing Su, Zhi Zhang, Yuhao Xiong
Summary: This study investigates the co-catalytic capabilities of MoO3 nanosheets in enhancing the enzyme-like catalytic activity of a two-dimensional ultrathin Fe(III)-modified covalent triazine framework (Fe-CTF) under neutral pH conditions. The Fe-CTF/MoO3 co-catalytic system exhibits enzyme-mimicking activity and enables the development of a colorimetric method for glucose detection. Furthermore, a straightforward one-pot colorimetric method is established for screening XOD inhibitors.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Lichao Zhang, Kang Xiao, Xueting Wang, Liang Kong
Summary: A novel fusion technology was designed to identify FAD-binding sites, achieving the best results on two independent test datasets and outperforming existing methods significantly. The high performance and certainty of the method were demonstrated through statistical tests and cross-entropy loss analysis.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Abdulkadir Kilic, Mehmet Aslan, Abdulkadir Levent
Summary: This study developed a simple, rapid, sensitive, and selective voltammetric technique for the electrochemical characterization and detection of the highly risky drug Edoxaban. The optimized voltammetric method showed good analytical working range and was successfully applied to urine and tablet samples.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Su Hyun Kim, Nara Shin, Jong-Min Jeon, Jeong-Jun Yoon, Jeong Chan Joo, Hee Taek Kim, Shashi Kant Bhatia, Yung-Hun Yang
Summary: To address environmental issues caused by traditional plastics, bioplastics have gained attention as alternatives. Although bioplastics have better degradability, their degradation still takes longer than anticipated. This study proposes a novel screening method to identify bioplastic degraders faster, saving time and providing more quantitative data.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Irem Okman Kocoglu, Pinar Esra Erden, Esma Kilic
Summary: In this study, an electrochemical biosensor based on carbon nanofibers and ionic liquid modification was constructed for tyramine detection. The biosensor showed linear response, low detection limit, high sensitivity, and exhibited good reproducibility, stability, and anti-interference ability.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Liana U. Akhmetzianova, Timur M. Davletkulov, Assol R. Sakhabutdinova, Alexey Chemeris, Irek M. Gubaydullin, Ravil R. Garafutdinov
Summary: A new program called LAMPrimers iQ has been developed for high-quality LAMP primer design, and its advantages in providing higher specificity and reliable detection of viral RNA were validated using SARS-CoV-2 coronavirus RNA as a model target.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Tommaso Pileri, Alberto Sinibaldi, Agostino Occhicone, Norbert Danz, Elena Giordani, Matteo Allegretti, Frank Sonntag, Peter Munzert, Patrizio Giacomini, Francesco Michelotti
Summary: This study developed a biosensing device based on one-dimensional photonic crystal to detect HER2 in breast cancer. The device combines label-free and fluorescence operation modes, allowing for real-time and accurate detection in less than 20 minutes. It offers a promising technique for combined label-free and fluorescence detection in disease diagnosis and therapeutic monitoring.
ANALYTICAL BIOCHEMISTRY
(2024)
