Article
Chemistry, Multidisciplinary
Adela Musilova, Jiri Vojta, Tomas Krizek, Anna Kubickova, Pavel Coufal
Summary: This work investigates the application of newly developed poly(styrene-divinylbenzene-methacrylic acid) monolithic columns in capillary electrochromatographic and capillary liquid chromatographic separations of low-molecular-weight organic compounds. The study examines the influence of polymerization time on chromatographic properties of the prepared capillary columns, and investigates the effect of electrostatic interactions between methacrylic acid and selected analytes on their retention in capillary liquid chromatography. The results demonstrate the high reliability of the prepared separation media.
MONATSHEFTE FUR CHEMIE
(2022)
Article
Biochemical Research Methods
A. S. Popov, K. A. Spiridonov, A. S. Uzhel, A. D. Smolenkov, A. Chernobrovkina, A. Zatirakha
Summary: Evaluation of the chromatographic properties of covalently bonded hyperbranched stationary phase based on PS-DVB in suppressed IC, RP HPLC, and HILIC modes showed good separation performance for various compounds. Studying the retention trends of hydrophobic and polar analytes on the prepared stationary phase revealed the significant influence of analyte nature on the retention mechanism.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Chemistry, Analytical
Junwei Liu, Meili Zhang, Qidong Zhang, Jian Mao, Yu-an Sun, Shusheng Zhang, Yan Zhu, Yinlong Guo, Jianxun Zhang
Summary: In this study, graphitic carbon-nitride (g-C3N4) nanosheets agglomerated poly (styrene-divinylbenzene) (PSDVB) anion-exchange stationary phase was prepared for use in ion chromatography. The self-fabricated column showed successful separation of six conventional anions and four monovalent organic acids, demonstrating good reactivity and baseline separation.
MICROCHEMICAL JOURNAL
(2021)
Article
Chemistry, Analytical
Juliana Soares da Silva Burato, Joao Victor Basolli Borsatto, Fernando Mauro Lancas
Summary: Using open tubular (OT) capillary columns, specifically the PLOT (porous layer open tubular) type, offers advantages in terms of interaction with analytes in liquid chromatography. This study explores the application of PLOT columns in capillary LC and discusses the preparation, optimization, and evaluation of these columns. Poly(styrene-divinylbenzene) (PS-DVB) was used as the stationary phase, and comparisons were made between columns with internal diameters of 10 and 25 μm. The effects of flow rate and gradient window were also investigated.
Review
Chemistry, Analytical
Isabela de Paula Lima, Sandy Polycarpo Valle, Marcone Augusto Leal de Oliveira, Flavia Ferreira de Carvalho Marques, Fernando Antonio Simas Vaz
Summary: Monolithic stationary phases have been widely studied in separation science, particularly in liquid chromatography and capillary electrochromatography, for over three decades. The production of monolithic columns involves various steps, including precursor solution preparation, tubing pretreatment, solution introduction, in situ reaction, and posttreatment. Different types of processes and reactions, such as sol-gel, polycondensation, free radical polymerization, and click reactions, are discussed. This work aims to provide an overview of the production process and the characteristics of monolith compositions, as well as to encourage researchers in finding suitable monoliths for analytical separations.
MICROCHEMICAL JOURNAL
(2023)
Review
Biochemical Research Methods
Hassan Y. Aboul-Enein, Valliappan Kannappan, Selvakumar Kanthiah
Summary: This review paper focuses on the recent development of monolithic chiral stationary phases (CSPs) in liquid chromatography (LC), with an emphasis on polysaccharide and cyclodextrin-based monolithic CSPs. It also discusses competing techniques and the application of monolithic chiral CSPs in LC and capillary electrochromatography.
COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING
(2023)
Article
Chemistry, Analytical
Lacey S. Billotto, R. Kenneth Marcus
Summary: A trilobal capillary-channeled polymer fiber stationary phase was evaluated for intact protein separations and compared to commercially-available columns. The fiber columns demonstrated faster separation times and gradient rates with comparable chromatographic performance to the commercial columns.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Chemistry, Analytical
Marwa A. El-Wahab Mubarak, Ramzia El-Bagary, M. Abdul-Azim Mohammad, Nisreen F. Abo-Talib, Ehab F. Elkady
Summary: Two simple, sensitive, and rapid liquid chromatographic methods were developed for the determination of palonosetron hydrochloride and its related impurities. Both Method A and Method B showed excellent linearity and detection limits, making them suitable for quality control of palonosetron in pharmaceutical dosage forms.
MICROCHEMICAL JOURNAL
(2021)
Article
Chemistry, Analytical
Rafael Marques, Nerilson M. Lima, Luiz Felipe Cantarino, Gustavo S. G. de Carvalho, Alexandre A. Leitao, Claudimir Lucio do Lago, Marcone A. L. de Oliveira
Summary: This study prepared monolithic stationary phases for capillary chromatography by polymerizing MPTMS monomer, applied them in separating PAHs, and attempted to improve the stationary phase by incorporating BM as a comonomer. The physical homogeneity of the columns was evaluated using an optical microscope and a conductivity detector, while the chemical characterization was done using solid-state NMR spectroscopy.
MICROCHEMICAL JOURNAL
(2021)
Article
Chemistry, Physical
Ya. A. Klimova, L. D. Asnin
Summary: This study investigated the adsorption of water-methanol and water-acetonitrile mixtures on chiral stationary phases. The results showed similar adsorption patterns on different CSPs, with differences in quantitative indicators. The thickness of the adsorption phase and activity coefficients were calculated and discussed, revealing deviations from the properties of an ideal adsorption solution.
RUSSIAN JOURNAL OF PHYSICAL CHEMISTRY A
(2021)
Article
Polymer Science
A. Boborodea, P. Boulens
Summary: A novel fractionation method using xylene as an alternative to TCB was developed for a more comprehensive characterization of the chemical structure of HDPE. The method offers better signal-to-noise ratio and more applications, such as simultaneous measurement of wax content and molecular weight, as well as providing a fingerprint of the HDPE synthesis catalyst.
INTERNATIONAL JOURNAL OF POLYMER ANALYSIS AND CHARACTERIZATION
(2023)
Article
Chemistry, Applied
Weizheng Wang, Yaqi Wang, Feng Chen, Fuping Zheng
Summary: The study showed that after derivatization with PMP, the polarity of reducing sugars decreased, making C-18 column more suitable for separating reducing sugar-PMP derivatives, while amide column is more suitable for separating the original sugars.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2021)
Article
Biochemical Research Methods
Alessandra Biancolillo, Angelo Antonio D'Archivio
Summary: This study integrated computational molecular descriptors of saturated esters and poly(siloxane) stationary phases into QSRR models to predict solute retention indices. The models were generated using PLS regression and improved through variable selection and re-building. Results showed successful cross-column prediction and the suitability of the approach for deducing retention indices of unknown solutes in uncharted stationary phases.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Review
Chemistry, Analytical
Sebastiaan Eeltink, Daniel Meston, Frantisek Svec
Summary: This review highlights the current trends and recent advances in the field of organic polymer-based monolithic materials, covering literature published in 2020. It discusses historical background, protocols for anchoring monoliths covalently, tuning macroporous structure and its link to separation performance, as well as method development and key applications using novel monolithic columns.
ANALYTICAL SCIENCE ADVANCES
(2021)
Article
Biochemical Research Methods
Otar Sh. Gogolishvili, Elena N. Reshetova
Summary: The study focused on the retention patterns and adsorption thermodynamics of enantiomers of aromatic hydroxy acids on different chiral stationary phases, revealing that the retention mechanism varies with eluent pH. The research also showed that ion-ion interactions determine the retention and separation selectivity of hydroxy acid enantiomers on some CSPs, while nonionic interactions play a key role on others. Additionally, the anionic form of hydroxy acids primarily regulates the enantioselectivity of a specific CSP.
Article
Biochemistry & Molecular Biology
Constantin Bloechl, Christoph Holzner, Michela Luciano, Renate Bauer, Jutta Horejs-Hoeck, Ulrich Eckhard, Hans Brandstetter, Christian G. Huber
Summary: This study re-evaluates the proteolytic properties of Streptococcal pyrogenic exotoxin B (SpeB) and identifies potential host substrates involved in streptococcal infection. The research provides insights into the activation and specificity of SpeB and contributes to understanding its role in infection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemical Research Methods
Di Wang, Tao Zhang, Katarina Madunic, Antonius A. de Waard, Constantin Blochl, Oleg A. Mayboroda, Marieke Griffioen, Robbert M. Spaapen, Christian G. Huber, Guinevere S. M. Lageveen-Kammeijer, Manfred Wuhrer
Summary: This study performed a detailed analysis of GSL glycans in 19 AML cell lines and revealed significant differences in GSL glycan profiles among different cell lines. The abundance of GSL glycans was found to be associated with the transcriptomic expression of specific transcription factors and glycosyltransferases, providing insights into the regulation of GSL expression.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Plant Sciences
Martina Althammer, Christof Regl, Klaus Herburger, Constantin Blochl, Elena Voglas, Christian G. Huber, Raimund Tenhaken
Summary: Galactose toxicity (Gal-Tox) is a common phenomenon in plants, but little is known about the mechanism behind it. This study investigated the role of galactose-containing metabolites like galactose-1-phosphate (Gal-1P) and UDP-Gal in Gal-Tox. Overexpressing USP from Pisum sativum helped reduce Gal-1P levels and alleviate Gal-Tox symptoms in roots. However, the overexpression lines became more sensitive to exogenous galactose. This could be due to the elevated levels of UDP-Gal in the roots.
Article
Biochemistry & Molecular Biology
Fausto Gueths Gomes, Andre Cronemberger Andrade, Martin Wolf, Sarah Hochmann, Linda Krisch, Nicole Maeding, Christof Regl, Rodolphe Poupardin, Patricia Ebner-Peking, Christian G. Huber, Nicole Meisner-Kober, Katharina Schallmoser, Dirk Strunk
Summary: This study isolated extracellular vesicles (EVs) from platelet lysate and characterized them using various techniques. The researchers found that TFF-EVs derived from platelets had better functionality in skin-organoid formation and T-cell proliferation inhibition compared to other isolation methods. Additionally, the study revealed that the protein corona formed on TFF-EVs improved their functionality.
Article
Chemistry, Analytical
Katharina Boettinger, Wolfgang Esser-Skala, Marius Segl, Christoph Herwig, Christian G. Huber
Summary: The study used a simple HPLC-MS workflow to analyze mAb product quality in real-time at different fermentation time points. The results revealed the presence of misassembled variants in the fermentation broth, alongside changes in mAb glycosylation profile over time, with galactosylated variants being the most abundant. The workflow proved to be robust for analyzing the complex matrix with low standard deviations.
ANALYTICA CHIMICA ACTA
(2022)
Article
Cell Biology
Martin Wolf, Rodolphe W. Poupardin, Patricia Ebner-Peking, Andre Cronemberger Andrade, Constantin Bloechl, Astrid Obermayer, Fausto Gueths Gomes, Balazs Vari, Nicole Maeding, Essi Eminger, Heide-Marie Binder, Anna M. Raninger, Sarah Hochmann, Gabriele Brachtl, Andreas Spittler, Thomas Heuser, Racheli Ofir, Christian G. Huber, Zami Aberman, Katharina Schallmoser, Hans-Dieter Volk, Dirk Strunk
Summary: Nanoparticles derived from therapy-grade human placental-expanded stromal cells are surrounded by a protein corona, which improves skin regeneration and angiogenesis and induces differential signaling in immune cells.
JOURNAL OF EXTRACELLULAR VESICLES
(2022)
Article
Biochemistry & Molecular Biology
Isabel J. Hoppe, Bernhard Prommegger, Andreas Uhl, Urs Lohrig, Christian G. Huber, Hans Brandstetter
Summary: This study introduces a fluorescence-based enzyme cascade method that can detect chemical and conformational changes in protein samples and establish databases of modification-function relationships for proteomic research.
Article
Medicine, Research & Experimental
Arthur Hinterholzer, Jennifer Moises, Christof Regl, Sebastian Schwap, Erdmann Rapp, Christian G. Huber, Mario Schubert
Summary: This article presents a straightforward NMR approach to detect the presence of alpha-Gal in biotherapeutics. The method utilizes sensitive spectra for quick screening and confirmation, allowing for effective monitoring and minimization of adverse effects.
Article
Chemistry, Multidisciplinary
Jennifer E. E. Moises, Christof Regl, Arthur Hinterholzer, Christian G. G. Huber, Mario Schubert
Summary: Glycation is a non-enzymatic and spontaneous post-translational modification that needs to be carefully monitored. 2D NMR spectroscopy can be used to identify glucose-induced glycation products.
PHARMACEUTICAL RESEARCH
(2023)
Article
Cell Biology
Melanie Schuerz, Joachim Danmayr, Maria Jaritsch, Eva Klinglmayr, Heloisa Melo Benirschke, Cristian-Tudor Matea, Patrick Zimmerebner, Jakob Rauter, Martin Wolf, Fausto Gueths Gomes, Zdenek Kratochvil, Zbynek Heger, Andrew Miller, Thomas Heuser, Vesna Stanojlovic, Jana Kiefer, Tanja Plank, Litty Johnson, Martin Himly, Constantin Bloechl, Christian G. Huber, Martin Hintersteiner, Nicole Meisner-Kober
Summary: EVAnalyzer is an ImageJ/Fiji plugin for automated, quantitative single vesicle analysis from imaging data. It allows for rigorous characterisation of extracellular vesicles (EVs) using standard fluorescence microscopes and accessible tools. The plugin is based on open-source software and can be used with commonly available reagents and laboratory equipment.
JOURNAL OF EXTRACELLULAR VESICLES
(2022)
Article
Biochemistry & Molecular Biology
Christoph Holzner, Katharina Boettinger, Constantin Bloechl, Christian G. Huber, Sven O. Dahms, Elfriede Dall, Hans Brandstetter
Summary: The cysteine protease legumain can translocate to the cell surface through interaction with the RGD-dependent integrin receptor alpha V beta 3. Legumain processes the C-terminal linker region of the TrkB ectodomain, affecting TrkB-BDNF signaling. Legumain-processed TrkB still binds BDNF, suggesting a potential scavenger role of soluble TrkB towards BDNF.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Laura Urwanisch, Michael Stefan Unger, Helene Sieberer, Hieu-Hoa Dang, Theresa Neuper, Christof Regl, Julia Vetter, Susanne Schaller, Stephan M. Winkler, Emanuela Kerschbamer, Christian X. Weichenberger, Peter W. Krenn, Michela Luciano, Lisa Pleyer, Richard Greil, Christian G. Huber, Fritz Aberger, Jutta Horejs-Hoeck
Summary: Epigenetic alterations play a significant role in cancer development, and the reversible nature of epigenetic changes makes them attractive targets for therapy. In this study, the authors investigated the expression of HDAC genes in AML patients and cell lines, and the effects of the HDAC inhibitor TMP269 on AML cells. They found significant overexpression of various HDACs in AML and observed that TMP269 treatment had effects on the proteome and growth of AML cells. Additionally, they demonstrated that the combination of TMP269 and venetoclax resulted in enhanced cell apoptosis. These findings highlight the potential of TMP269 as a therapeutic compound for AML.
Article
Chemistry, Analytical
Fiammetta Di Marco, Gabriele Bluemel, Constantin Bloechl, Manfred Wuhrer, Christian G. Huber
Summary: This study characterized gonadotropins using mass spectrometry and identified over a thousand different glycoforms, highlighting the importance of glycosylation patterns for quality control of biopharmaceuticals.
ANALYTICA CHIMICA ACTA
(2023)
Article
Biochemistry & Molecular Biology
Pauline Defant, Christof Regl, Christian G. Huber, Mario Schubert
Summary: This article presents a study on glycation, a non-enzymatic modification that occurs when reducing sugars react with free amines in protein side chains. The researchers conducted a detailed NMR analysis to identify the correlation patterns of the Amadori product formed between proteins and maltose. The results demonstrate that NMR spectroscopy is an independent method for detecting maltose-based glycation, which can be validated with other methods.
JOURNAL OF BIOMOLECULAR NMR
(2023)
Article
Chemistry, Medicinal
Alaa Alhayek, Ahmed S. Abdelsamie, Esther Schoenauer, Virgyl Camberlein, Evelyn Hutterer, Gernot Posselt, Jamil Serwanja, Constantin Bloechl, Christian G. Huber, Jorg Haupenthal, Hans Brandstetter, Silja Wessler, Anna K. H. Hirsch
Summary: This study reports the discovery of two potent and chemically stable bacterial collagenase inhibitors, synthesized and FDA-approved diphosphonates and hydroxamates. These compounds showed high activity against clostridial and bacillary collagenases, demonstrating antibacterial effects.
JOURNAL OF MEDICINAL CHEMISTRY
(2022)