Review
Biochemistry & Molecular Biology
Andres Tabernilla, Bruna dos Santos Rodrigues, Alanah Pieters, Anne Caufriez, Kaat Leroy, Raf Van Campenhout, Axelle Cooreman, Ana Rita Gomes, Emma Arnesdotter, Eva Gijbels, Mathieu Vinken
Summary: The paper proposes a rationalized tiered in vitro testing strategy to detect liver toxicity triggered by chemicals, with the first tier focused on general cytotoxicity assessment and the second tier aimed at identifying liver-specific toxicity. It provides an overview of commonly used in vitro assays and discusses the advantages, disadvantages, and practical considerations of each assay.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Polymer Science
Fatma Nur Parin, Sana Ullah, Kenan Yildirim, Motahira Hashmi, Ick-Soo Kim
Summary: The study developed efficient hydrophilic composite nanofibers loaded with folic acid through electrospinning and electrospraying processes and analyzed their properties, release characteristics, and cytocompatibility. The results indicate the potential application of these nanofibers in skin-care and tissue engineering.
Review
Medicine, Research & Experimental
Chun Y. J. Wong, Hui Xin Ong, Daniela Traini
Summary: The use of e-cigarettes among adolescents has become increasingly popular, but studying their toxicological profile using in vitro models presents challenges. This review discusses the current status of e-cigarette assays in vitro, explores various cellular assays used to evaluate the safety of e-cigarettes, and provides future research directions.
Article
Chemistry, Applied
Bianca Stoean, Luiza Gaina, Castelia Cristea, Radu Silaghi-Dumitrescu, Adrian M. V. Branzanic, Monica Focsan, Eva Fischer-Fodor, Bogdan Tigu, Cristian Moldovan, Andra Diana Cecan, Patriciu Achimas-Cadariu, Simion Astilean, Luminita Silaghi-Dumitrescu
Summary: In this study, two new phenothiazinium dyes with N-piperidinyl-carbinol auxochrome units were designed, synthesized, and evaluated for fluorescence imaging and cytotoxicity in ovarian cancer cells. A solvent-free mechanochemical method was used for the synthesis of the dyes, providing a greener alternative to the traditional solution-based synthesis. The optical properties of the dyes were characterized by UV-vis absorption/emission spectroscopy, showing red-shifted absorption and emission peaks in more polar solvents. Theoretical DFT calculations were performed, and the results were in good agreement with the experimental data. The new dyes exhibited hydrophilic properties and showed selective staining of the cytoplasm of tumoral cells. They also displayed moderate cytotoxicity against ovarian cancer cell lines, with one of the dyes showing a deeper influence on the function of chemoresistant cells.
Article
Pharmacology & Pharmacy
Jwala Renukuntla, Sushesh Srivatsa Palakurthi, Pradeep Kumar Bolla, Bradley A. Clark, Sai H. S. Boddu, Prashanth Manda, Samuel Sockwell, Nitin B. Charbe, Srinath Palakurthi
Summary: The USFDA requires the generic industry to prove the pharmaceutical and bioequivalence of topical ocular products. Unlike generic oral drugs, proving bioequivalence for topical ocular products has been challenging and requires multiple in-vitro tests. This review focuses on the current status of biowaiver criteria for complex ophthalmic products.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Review
Physiology
Giorgia Palano, Ariana Foinquinos, Erik Mullers
Summary: Cardiac fibrosis, characterized by excessive deposition of extracellular matrix components, leads to pathological remodeling and dysfunction of the heart. Effective therapies targeting cardiac fibrosis are lacking due to limited understanding of the mechanisms. Improvements in in vitro models and high-content analysis methods are expected to enhance predictivity and screening efficiency for anti-fibrotic compounds.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Multidisciplinary Sciences
Renate Krassnig, Gloria Hohenberger, Angelika Schwarz, Walter Goessler, Gebhard Feierl, Renate Wildburger, Reinhard Windhager
Summary: The study concluded that adding nanosilver or silver sulfate to bone cement does not release bactericidal concentrations of silver (I) ions. However, adding certain concentrations of silver sulfate to bone cement is effective as a temporary spacer.
SCIENTIFIC REPORTS
(2021)
Article
Food Science & Technology
Veronica Zingales, Noemi Torriero, Luca Zanella, Monica Fernandez-Franzon, Maria-Jose Ruiz, Maria Rosaria Esposito, Elisa Cimetta
Summary: This study used an in vitro 3D spheroid model from human neuroblastoma cells to evaluate the cytotoxic effects of the mycotoxin STE. Results showed that STE induced cell viability decrease, cell disaggregation, apoptosis, and altered cell migration.
FOOD AND CHEMICAL TOXICOLOGY
(2021)
Review
Chemistry, Analytical
Marimuthu Citartan
Summary: Light-up aptamers are aptamers that trigger fluorescence emission of specific dyes upon binding, widely used in in vivo imaging and in vitro diagnostic assay development. This study aims to provide an overview of various diagnostic assay formats developed using light-up aptamers, offering insights for researchers interested in developing one-pot in vitro diagnostic assays based on light-up aptamers.
Article
Pharmacology & Pharmacy
Ana Sofia Agonia, Ana Palmeira-de-Oliveira, Catarina Cardoso, Catia Augusto, Christian Pellevoisin, Christelle Videau, Ricardo Jorge Dinis-Oliveira, Rita Palmeira-de-Oliveira
Summary: The use of in vitro human skin permeation tests is valuable for evaluating the quality and equivalence of topical drug products. In this study, the use of reconstructed human epidermis (RhE) as a biological membrane for bioequivalence testing of clotrimazole creams demonstrated its discriminatory power and higher permeability compared to human skin.
Article
Biochemical Research Methods
Kaisong Yuan, Victor de la Asuncion-Nadal, Carmen Cuntin-Abal, Beatriz Jurado-Sanchez, Alberto Escarpa
Summary: The portable device described in the study integrates micromotors for real-time fluorescence sensing of various biomarkers. The device was compared to a high-performance optical microscope and showed similar analytical features. Its versatility allows for future use with a variety of biomarkers and even multiplexed detection schemes.
Article
Environmental Sciences
Fei Zhou, Yanan Li, Yuanzhe Ma, Qin Peng, Zewei Cui, Yang Liu, Min Wang, Hui Zhai, Nanchun Zhang, Dongli Liang
Summary: The study found that in seleniferous areas, selenium in soil mainly exists in stable forms, and the selenium content in crops is significantly correlated with organic-bound and phosphate-extractable selenium content in soil. The different in vitro digestion methods showed consistent results in determining selenium bioaccessibility in soil and crops, with PBET method considered as the optimum approach.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Dentistry, Oral Surgery & Medicine
Julia Guerrero-Girones, Sergio Lopez-Garcia, Miguel R. Pecci-Lloret, Maria P. Pecci-Lloret, Francisco Javier Rodriguez Lozano, David Garcia-Bernal
Summary: This study assessed and compared the in vitro biocompatibility of different resins used for dental splints. The results showed that the 3D-printed resins and conventional resin had similar biocompatibility, except for Freeprint Splint, which was the most cytotoxic on hGFs.
JOURNAL OF DENTISTRY
(2022)
Article
Biochemistry & Molecular Biology
Pedro Herreros, Silvia Tapia-Gonzalez, Laura Sanchez-Olivares, Maria Fe Laguna Heras, Miguel Holgado
Summary: This study presents a brain slice-on-a-chip system with an integrated injection system for long-term monitoring of fluorescent dye in living cells. The technology developed in this study improves local administration of reagents and could serve as a highly effective bioreactor in organotypic cultures.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, Research & Experimental
Nicholas van Eijk, Luna C. Schmacke, Torsten Steinmetzer, Oliver Pilgram, Miklos Poor, Erzsebet Paszti-Gere
Summary: The outbreak of COVID-19 has accelerated the development of small molecule antivirals that target TTSPs involved in the host-cell entry of viruses. Researchers evaluated a set of enzyme inhibitors in primary human hepatocyte models using various assays to assess their cytotoxicity, redox imbalance, protein interactions, and selectivity towards key proteases. One inhibitor (MI-1903) was found to be detrimental to cell viability, but others showed no negative effects, with MI-21 demonstrating the best selectivity and potential. These in vitro experiments aid in selecting compounds with optimal selectivity and safety profiles.
BIOMEDICINE & PHARMACOTHERAPY
(2023)