Article
Chemistry, Applied
Junyu Wang, Wenjie Li, Yaqing Wang, Tushar Pavase, Jiukai Zhang, Zhenxing Li, Hong Lin
Summary: Food allergy poses a serious health risk worldwide and the development of a sesame allergen detection method is crucial. The sandwich ELISA method can sensitively and efficiently detect foods containing sesame ingredients, protecting sensitive individuals from exposure.
FOOD AND AGRICULTURAL IMMUNOLOGY
(2021)
Article
Materials Science, Multidisciplinary
Bo Kyeong Yoon, Tun Naw Sut, Ki Yeol Yoo, Seung Hwa Lee, Youngkyu Hwang, Joshua A. Jackman, Nam-Joon Cho
Summary: A streamlined ELISA strategy using lipid nanoparticles as a blocking agent was developed, showing fewer processing steps, quicker protocol time, and superior detection performance compared to conventional BSA blocking. This method has been successfully applied to COVID-19 antibody detection and is broadly applicable for rapid ELISA diagnostics development.
APPLIED MATERIALS TODAY
(2021)
Article
Multidisciplinary Sciences
Youhee Heo, Kwanwoo Shin, Min Cheol Park, Ji Yoon Kang
Summary: This report introduces a method for enhancing the sensitivity of chemifluorescence-based ELISA, using photooxidation-induced fluorescence amplification (PIFA). PIFA utilizes autocatalytic photooxidation to amplify the fluorescent product, improving detection sensitivity. The method requires minimal modifications to the fluorescence reader and is compatible with conventional ELISA assays.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Applied
Long Xu, Fanfan Yang, Alberto C. P. Dias, Xiaoying Zhang
Summary: Sunset yellow is a commonly used food colorant, but excessive and illegal use may pose health risks. Researchers have applied quantum dots in the analysis of sunset yellow and developed two new detection methods. These methods are efficient, highly sensitive, and capable of distinguishing sunset yellow from analogues.
Article
Plant Sciences
Jun You, Donghua Li, Li Yang, Senouwa Segla Koffi Dossou, Rong Zhou, Yanxin Zhang, Linhai Wang
Summary: The CRISPR/Cas9 system was successfully applied to sesame to induce targeted mutagenesis in specific genes. The results demonstrate the efficiency and effectiveness of CRISPR/Cas9 coupled with hairy root transformation in assessing gene functions in sesame. This study provides important insights into gene functional research and molecular breeding in sesame.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Biochemistry & Molecular Biology
Mebeaselassie Andargie, Maria Vinas, Anna Rathgeb, Evelyn Moeller, Petr Karlovsky
Summary: This article reviews the characteristics and transformation pathways of lignans in sesame, including their biosynthesis processes and effects in plants. Studies have found lignans to be beneficial for health, with properties that promote health and enhance the efficacy of insecticides, but the specific biological functions in plants still need further research.
Article
Food Science & Technology
Kossivi Fabrice Dossa, Anselm Anibueze Enete, Yann Emmanuel Miassi, Abiodun Olusola Omotayo
Summary: This study analyzed the economic characteristics of sesame produced in the northern region of the Republic of Benin. The findings showed that sesame farming was profitable in the study area but faced challenges such as labor and land scarcity, uneven ripening, lack of storage facilities, and access to improved seed.
FRONTIERS IN SUSTAINABLE FOOD SYSTEMS
(2023)
Review
Biotechnology & Applied Microbiology
Micheale Yifter Weldemichael, Hailay Mehari Gebremedhn
Summary: This paper reviews recent advances in QTL mapping for yield and yield-related traits in sesame, focusing on the identification and mapping of potential candidate genes/QTLs associated with desirable traits, as well as the potential genetic resources for molecular marker-assisted selection and functional gene cloning.
JOURNAL OF BIOTECHNOLOGY
(2023)
Article
Food Science & Technology
Nicki Shwe Yee, Qianqian Shao, Chatchporn Uraipong, Masahiro Shoji, Nanju Alice Lee
Summary: The majority of imported pre-packaged food products from Mainland China in Sydney, Australia complied with food standards and allergen labeling requirements, with milk, eggs, wheat, and peanuts being the most prevalent allergens posing potential risks for allergic consumers. Some products contained PAL statements and allergen residues, indicating possible hidden allergen risks.
Review
Agronomy
Desawi Hdru Teklu, Hussein Shimelis, Seltene Abady
Summary: Sesame production is hampered by various factors, but advancements in breeding and genetic techniques offer opportunities for improving yield and quality. Integration of traditional breeding methods with genomic techniques can accelerate the development of sesame varieties with market-preferred traits.
Article
Plant Sciences
Brij Bihari Pandey, P. Ratnakumar, B. Usha Kiran, Mangesh Y. Dudhe, G. Sowjanya Lakshmi, Kulasekaran Ramesh, Arti Guhey
Summary: This study investigated the trait variation in sesame under irrigated and terminal drought stress conditions using 76 accessions from the Indian core set. The traits associated with seed yield differed between WW and WS conditions, with certain traits showing positive correlations with seed yield and others showing negative correlations. Genotypes with lower canopy temperature, higher relative water content, and higher harvest index under WS had the highest seed yields. Phenotypic and molecular diversity analysis revealed promising genotypes that can be used in breeding programs for developing new varieties.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Chemistry, Analytical
Jie Fei, Jiaqun Jiang, Yuchen Bai, Weilin Wu, Xiangjun Zhao, Wenbo Yu, Kai Wen, Xuezhi Yu, Jianzhong Shen, Zhanhui Wang
Summary: This study demonstrates the importance of careful hapten design and extensive antibody screening in the development of sandwich immunoassays for small molecules, using ABM as an example. Through combinatorial associations of antibody pairs, a sandwich immunoassay for ABM was successfully established.
ANALYTICAL CHEMISTRY
(2023)
Review
Nutrition & Dietetics
Panpan Wei, Fenglan Zhao, Zhen Wang, Qibao Wang, Xiaoyun Chai, Guige Hou, Qingguo Meng
Summary: Sesame, a widely cultivated and popular oil crop, has high nutritional value and numerous health benefits due to its richness in protein and lipids. Its active components have been found to possess antioxidant, cholesterol-reducing, anti-tumor, and other effects. Sesame has various applications in food, feed, cosmetics, and health products, and its further development for more functionalities is supported by research on its nutritional value, chemical composition, pharmacological effects, and processing uses.
Article
Chemistry, Analytical
Hui Li, Menglu Li, Yucai Yang, Fei Wang, Feng Wang, Chao Li
Summary: A direct method has been described to establish the correlation between non-nucleic acid analytes and the CRISPR/Cas12a system using rationally designed DNA strands, enabling ultrasensitive detection of biomarkers from different species. The signal output is highly optional and the sensing principle is akin to ELISA, allowing for direct application on the currently available ELISA platform, further facilitating its use in medical diagnostics.
ANALYTICAL CHEMISTRY
(2021)
Review
Agronomy
Muhammad Usman, Maryam Razzaq, Rana Ahsan Rehman Khan, Muhammad Abdur Rehman, Muhammad Moaaz Ali, Shaista Gull, Ahmed Fathy Yousef, Muhammad Adnan, Sezai Ercisli, Kirill S. Golokhvast
Summary: Current postharvest activities in the sesame value chain result in significant losses, reducing overall global productivity. Insufficient use of adequate harvesting, packaging, and handling technologies by farmers, as well as a lack of strategies for minimizing postharvest losses, contribute to the decrease in productivity. This study emphasizes the importance of increasing production and quality while implementing mitigation strategies to reduce postharvest losses as the only solution to bridge the gap between global productivity and the potential production of sesame.
Article
Chemistry, Applied
Maria Kaltenbrunner, Rupert Hochegger, Margit Cichna-Markl
Article
Chemistry, Applied
Maria Kaltenbrunner, Rupert Hochegger, Margit Cichna-Markl
Article
Food Science & Technology
Maria Kaltenbrunner, Rupert Hochegger, Margit Cichna-Markl
Article
Biochemistry & Molecular Biology
Samuel M. Meier-Menches, Katja Zappe, Andrea Bileck, Dominique Kreutz, Ammar Tahir, Margit Cichna-Markl, Christopher Gerner
Article
Chemistry, Applied
Stefanie Dobrovolny, Marion Blaschitz, Thomas Weinmaier, Jan Pechatschek, Margit Cichna-Markl, Alexander Indra, Peter Hufnagl, Rupert Hochegger
Article
Multidisciplinary Sciences
Maria Kaltenbrunner, Walter Mayer, Kirsten Kerkhoff, Rita Epp, Hermann Rueggeberg, Rupert Hochegger, Margit Cichna-Markl
SCIENTIFIC REPORTS
(2019)
Article
Biochemistry & Molecular Biology
Georg Pretsch, Nasim Sanadgol, Roman Smidak, Jana Lubec, Volker Korz, Harald Hoeger, Katja Zappe, Margit Cichna-Markl, Gert Lubec
Article
Chemistry, Multidisciplinary
Samuel M. Meier-Menches, Benjamin Neuditschko, Katja Zappe, Martin Schaier, Marlene C. Gerner, Klaus G. Schmetterer, Giorgia Del Favero, Riccardo Bonsignore, Margit Cichna-Markl, Gunda Koellensperger, Angela Casini, Christopher Gerner
CHEMISTRY-A EUROPEAN JOURNAL
(2020)
Article
Multidisciplinary Sciences
Maria Kaltenbrunner, Walter Mayer, Kirsten Kerkhoff, Rita Epp, Hermann Rueggeberg, Rupert Hochegger, Margit Cichna-Markl
SCIENTIFIC REPORTS
(2020)
Review
Cell Biology
Katja Zappe, Margit Cichna-Markl
Article
Food Science & Technology
Kristina Gense, Verena Peterseil, Alma Licina, Martin Wagner, Margit Cichna-Markl, Stefanie Dobrovolny, Rupert Hochegger
Summary: The study introduces a DNA metabarcoding method for identifying bivalve species in foodstuffs, showing high accuracy in detecting both major and minor components. It is suitable for complex and processed foods, providing a useful tool for food authentication.
Article
Food Science & Technology
Barbara Druml, Steffen Uhlig, Kirsten Simon, Kirstin Frost, Karina Hettwer, Margit Cichna-Markl, Rupert Hochegger
Summary: A real-time PCR assay has been developed to identify and quantify roe deer in food products, aimed at detecting potential adulteration of game meat with cheaper alternatives. Interlaboratory ring trials across several EU Member States have shown the assay to be reliable and accurate for the detection and quantification of roe deer in raw meat samples. Performance characteristics such as amplification efficiency, LOD95%, repeatability, reproducibility, and accuracy of quantitative results have been evaluated with satisfactory results, making this PCR assay a valuable tool for food authentication purposes.
Article
Food Science & Technology
Laura Preckel, Claudia Brunen-Nieweler, Gregoire Denay, Henning Petersen, Margit Cichna-Markl, Stefanie Dobrovolny, Rupert Hochegger
Summary: DNA metabarcoding is an effective method for routine analysis of mammalian and poultry species in food and pet food products, showing consistent results with PCR analysis. It is particularly suitable for detecting unexpected species and is a cost-effective alternative, especially for analyzing pet food products with high potential for adulteration.
Article
Food Science & Technology
Stefanie Dobrovolny, Steffen Uhlig, Kirstin Frost, Anja Schlierf, Kapil Nichani, Kirsten Simon, Margit Cichna-Markl, Rupert Hochegger
Summary: Meat species authentication in food is often conducted using genetic variation detection. DNA metabarcoding, a novel method that can simultaneously identify various species, shows great potential in the field and could be harmonized across EU Member States.
Editorial Material
Food Science & Technology
Margit Cichna-Markl, Isabel Mafra