Article
Chemistry, Inorganic & Nuclear
Jack C. Dorrat, Rosemary J. Young, Christopher G. P. Taylor, Max B. Tipping, Andrew J. Blok, David R. Turner, Alasdair I. McKay, Simon Ovenden, Michael D. Ward, Genevieve H. Dennison, Kellie L. Tuck
Summary: This study investigates the host-guest chemistry between octanuclear cubic coordination cage hosts and O,O'-diisopropyl fluorophosphate (DFP), a simulant of phosphonofluoridate G-series chemical warfare agent. The results show that two coordination cages, H-PEG and H-W, can increase the lifetime of DFP in aqueous buffer solution by protecting it from hydrolysis. Crystallographic analysis reveals that DFP binds to the windows of the cage surface, with the phosphorus-fluorine bond directed into the internal cavity, enhancing the stability of DFP. Initial studies with the chemical warfare agent sarin (GB) also indicate a reduced rate of hydrolysis when bound in the H-PEG cage. These cages' ability to inhibit hydrolysis of P-F bond containing organophosphorus guests through encapsulation may have applications in forensic sample preservation and analysis.
DALTON TRANSACTIONS
(2023)
Article
Environmental Sciences
A. Amala Lourthuraj, Mohammad Rafe Hatshan, Dina S. Hussein
Summary: This study aimed to identify pesticide degrading bacteria and localize organophosphate hydrolase activity. Results showed that two bacteria isolated from contaminated samples were able to efficiently remove chlorpyrifos and utilize pesticides as a source of carbon and energy.
ENVIRONMENTAL RESEARCH
(2022)
Article
Nanoscience & Nanotechnology
Yunxia Yang, Qian Liu, Yisong Zhao, Jianmin Chen, Bing Chen, Yanling Yan, Faming Gao
Summary: This study explored CuPt alloy nanotubes with an open end, which have a larger electrochemical active surface area and more active sites for enzyme immobilization, thus improving the efficiency and sensitivity of the biosensor.
Article
Biochemistry & Molecular Biology
Jian-Hui Li, Xue-Lei Deng, Yu-Lian Zhao, Xiao-Yan Zhang, Yun-Peng Bai
Summary: This study presents an improved approach to immobilize a malathion degrading enzyme PoOPHM9 on paper, resulting in high enzyme activity capture and rapid colorimetric detection of malathion in a short period of time. The paper-based biosensors showed promising potential for on-site analysis of malathion-contaminated samples.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2021)
Article
Biophysics
K. Yugender Goud, Samar S. Sandhu, Hazhir Teymourian, Lu Yin, Nicholas Tostado, Frank M. Raushel, Steven P. Harvey, Lee C. Moores, Joseph Wang
Summary: This study presents a flexible printed textile-based solid-contact potentiometric sensor for selective detection of fluoride anions released by biocatalytic hydrolysis of G-type nerve agents, providing high sensitivity, excellent discrimination against common anions, and robust mechanical resilience. The sensor allows for the biosensing of nerve-agent simulants, with data transmission wirelessly to smartphones for real-time alerting, showing promise for effective discrimination of neurotoxins in defense settings.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Engineering, Chemical
Liang Yang, Xinying Zhang, Caifang Cao, Xiang Xue, Linsheng Wan
Summary: A new process for leaching low-grade scheelite using a mixture of sodium phosphate, sodium hydroxide, and calcium fluoride was proposed in this study, with thermodynamic analysis revealing conditions favorable for the formation of calcium fluorophosphate. The effects of various parameters on the leaching of low-grade scheelite were studied, and optimum process parameters were determined to achieve high tungsten leaching efficiency.
MINERALS ENGINEERING
(2022)
Review
Biochemistry & Molecular Biology
Himanshu Mali, Chandni Shah, Darshan H. Patel, Ujjval Trivedi, Ramalingam Bagavathi Subramanian
Summary: Organophosphate compounds are widely used as pesticides and chemical warfare agents, causing significant contamination of the environment and posing risks to human health. The development of safe and efficient methods for environmental restoration and detoxification is urgently needed. Organophosphate hydrolyzing enzymes have emerged as promising candidates for degrading these compounds. In this review, the prevalence of these enzymatic systems and recent advancements in enzyme engineering are discussed, highlighting their applications in detection, decontamination, and therapeutic detoxification. The challenges and future prospects in this field are also described.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2022)
Article
Chemistry, Analytical
Delun Zheng, Min Chen, Yaowen Chen, Wenhua Gao
Summary: CdCoS2(2) modified ITO electrode showed significantly improved photocurrent compared to CoS and CdS materials. The improved photo-absorption ability was attributed to the porous and hollow nature of CdCoS2(2) that promoted internal multilight scattering/reflection. The CdCoS2(2) heterostructure with close contact interface facilitated the separation/migration of photo-induced carriers. The addition of Ag nanoparticles further enhanced the PEC response. Acetylcholinesterase (AChE) immobilized on the CdCoS2(2)@Ag electrode surface showed elevated photocurrent output due to hydrolysis of acetylcholine chloride (ATCl) and production of thiocholine. The AChE/GLD-CS/CdCoS2(2)@Ag/ITO sensing platform achieved successful detection of chlorpyrifos with wide linear response range and low detection limit.
ANALYTICA CHIMICA ACTA
(2022)
Article
Materials Science, Multidisciplinary
Liang Yang, Chaoyang Li, Caifang Cao, Xiang Xue, Dandan Gong, Linsheng Wan
Summary: This study investigated the kinetics of low-grade scheelite leaching with a mixture of sodium phosphate and sodium fluoride. The results showed that the leaching rate of tungsten was greatly influenced by temperature and less affected by the concentration of phosphate and fluorine ions. The leaching process was controlled by a chemical reaction, and the reaction product was a loose, rod-like crystal.
Review
Biotechnology & Applied Microbiology
Lijie Wang, Yan Sun
Summary: The enzymatic degradation of organophosphorus compounds is an efficient and clean approach for detoxification and bioremediation, but further improvement in catalytic efficiency and resistance is needed. Research has focused on enhancing enzyme performance through molecular mutations and engineering methods, as well as expanding substrate spectrum and designing artificial enzymes for extended applications.
BIOCHEMICAL ENGINEERING JOURNAL
(2021)
Article
Chemistry, Multidisciplinary
Dhruba Jyoti Sarkar, Saswata Goswami, Swarupananda Sahu, Bijay Kumar Behera, Pranaya Parida, Shyamal Chandra Sukla Das, Basanta Kumar Das, Partha Sarkar, Debabrata Pradhan, Subhankar Mukherjee, Souvik Pal, Vipul Bansal
Summary: In this study, polyvinyl alcohol (PVA) capped AgNPs (AgNPs@PVA) were used as a nanocarrier to fabricate an acetylcholinesterase (AChE) biosensor for the detection of organophosphate (OP) pesticides. The AChE enzyme was immobilized onto a drop casted AgNPs@PVA layer on a glassy carbon electrode (GCE) to fabricate the electrochemical biosensor. The biosensor measured the peak current at 0.6 V generated by the AChE-mediated hydrolysis of acetylthiocholine (ATCl) to thiocholine and its subsequent oxidation to dimer through differential pulse voltammetry (DPV). A model OP pesticide, malathion, was used to hinder the AChE hydrolysis activity of the sensor probe, and the inhibition effect was found to be proportional to the concentration of malathion. The fabricated biosensor showed linearity in the range of 0.01 to 1 ng/mL of malathion at optimal conditions, with a detection limit (LOD) of 2.6 pg/mL. The AChE biosensor exhibited good selectivity for OP pesticides and demonstrated efficiency in real sample analysis.
Article
Chemistry, Applied
Jakkrit Suwannachat, Apichart Saenchoopa, Wonn Shweyi Thet Tun, Rina Patramanon, Sakda Daduang, Jureerat Daduang, Sirinan Kulchat
Summary: An enzymatic electrochemical biosensor was developed for the indirect detection of organophosphates by measuring the inhibition of acetylcholinesterase. The biosensor showed enhanced performance with the use of a copper nanowires/reduced graphene oxide composite on a screen-printed carbon electrode. This sensor is useful for reliable analysis of chlorpyrifos.
Article
Nanoscience & Nanotechnology
Yunxia Yang, Yisong Zhao, Tingting You, Qian Liu, Yukun Gao, Huaxiang Chen, Penggang Yin
Summary: The study developed a novel AChE biosensor based on Au-Tb alloy nanospheres for rapid and sensitive detection of organophosphate pesticides. The biosensor showed a wide linear range and low detection limit for methyl parathion, with high sensitivity, outstanding repeatability, and superior stability compared to other reported biosensors. The modified biosensor demonstrated satisfactory recoveries in tap water and lake samples, proving its feasibility for detecting OPs in real samples.
Review
Engineering, Environmental
Archana Mishra, Jitendra Kumar, Jose Savio Melo, Bhanu Prakash Sandaka
Summary: Dichlorvos is an organophosphate pesticide widely used in agriculture and industry, but its indiscriminate use can have harmful effects on the environment and human health. Research on detecting and monitoring dichlorvos has mainly focused on the past three decades, particularly in food, environment, and agriculture.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2021)
Article
Biochemistry & Molecular Biology
Nayana Bhat, Bodee Nutho, Chonnikan Hanpaibool, Sarinya Hadsadee, Alisa Vangnai, Thanyada Rungrotmongkol
Summary: This study investigated the binding of different organophosphates (OPs) pesticides to methyl parathion hydrolase (MPH) with cobalt(II) metal ions using computational modeling and molecular dynamics simulations. The findings suggest that the coordination patterns between the pesticides and metal ions differ, indicating a potential impact of metal ions on the functional evolution and divergence within enzyme superfamilies.
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS
(2023)
Article
Chemistry, Analytical
Yujia Ying, Huilin Li
Summary: Enzymes play a crucial role as biological catalysts in accelerating biochemical reactions in living organisms. This study proposes a convenient method for monitoring enzymatic catalytic processes using native top-down mass spectrometry. By exploring the heterogeneity of the chymotrypsin sample and using tandem mass spectrometry, the researchers were able to monitor covalent and noncovalent enzymatic complexes, substrates, and products during catalysis. The results demonstrate that this method has the potential to be a promising tool for characterizing biocatalysts.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Kang Yang, Shuaibo Shi, Jinyu Wu, Shaolong Han, Shengdi Tai, Shishen Zhang, Kun Zhang
Summary: H2O and D2O are important analogues closely related to various industries and monitoring fields. This study successfully distinguishes and detects H2O and D2O by designing a novel eu(III)-macrocycle with dual emitters, Eu-2a, and conducting fluorescence titration experiments.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Julia Kuligowski, Alvaro Perez-Rubio, Marta Moreno-Torres, Polina Soluyanova, Judith Perez-Rojas, Ivan Rienda, David Perez-Guaita, Eugenia Pareja, Ramon Trullenque-Juan, Jose Castell, Marcha Verheijen, Florian Caiment, Ramiro Jover, Guillermo Quintas
Summary: This study introduces a novel variable selection approach called cluster PLS (c-PLS) to assess the joint impact of variable groups selected based on biological characteristics on the predictive performance of a multivariate model. The usefulness of c-PLS is shown using miRNomic and metabolomic datasets obtained from the analysis of liver tissue biopsies.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Meixue Lai, Lijie Zhong, Siyi Liu, Yitian Tang, Tingting Han, Huali Deng, Yu Bao, Yingming Ma, Wei Wang, Li Niu, Shiyu Gan
Summary: This study presents a method for constructing wearable sweat electrolyte sensors using carbon fiber-based solid-contact ion-selective electrodes (SC-ISEs). By using carbon fibers extracted from commercial cloth as electrode material, the cost and reproducibility issues of flexible SC-ISEs were addressed. The results showed that the carbon fiber-based SC-ISEs exhibited reversible voltammetric and stable impedance performances, and had high reproducibility of standard potentials between normal and bending states.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Yang, Ying Liu, Min Deng, Peipei Wang, Dan Cheng, Songjiao Li, Longwei He
Summary: A near-infrared fluorescent probe has been developed to accurately measure the levels of peroxynitrite (ONOO-) in the endoplasmic reticulum (ER) in acute lung injury (ALI). The probe demonstrated rapid response, high selectivity, good sensitivity, and enhanced fluorescence intensity in response to ONOO-. It was successfully used to detect changes in ONOO- levels and showed significant increases in an ALI cell model and an ALI mouse model.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yanping Wang, Yuemei Chen, Kejun Li, Jinrong Zhou, Xin Yuan, Mei Zhang, Ke Huang
Summary: In this work, a portable analytical system based on point discharge chemical vapor generation atomic emission spectrometry (PD-CVG-AES) coupling with gold filament enrichment was designed. The highly sensitive analysis of Hg2+ indirectly realized the detection of ascorbic acid (AA). The measurement is based on the fact that Ag+ can decrease the concentration of Hg2+ by forming Ag-Hg amalgam in the presence of the reductant SnCl2, while AA can pre-reduce Ag+ to Ag0, leading to the generation of silver nanoparticles (Ag NPs). The developed novel analytical strategy broadens the application of microplasma-based AES and offers a higher level of sensitivity compared to current AA detection techniques.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yundi Huang, Bo Song, Kaiwen Chen, Deshu Kong, Jingli Yuan
Summary: This study developed two lysosome-targetable background-free TGL probes for efficient and accurate detection of 1O2, which can be used for monitoring endogenous 1O2 concentrations in lysosomes and discriminating variability induced by different photosensitizers. Furthermore, a smart luminescent sensor film was successfully prepared for on-site 1O2 production detection during PDT processes, offering a promising clinical monitoring tool for skin diseases.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Wei Lang, Jia-Mei Qin, Qian-Yong Cao
Summary: A novel polymer-based probe P1 was successfully synthesized for fluorescently ratiometric sensing of H2S with high selectivity and sensitivity. A smartphone sensing platform was constructed to conduct visual quantitative detection of H2S. P1 can be employed in evaluating the level fluctuations of H2S in living cells, testing water samples/wine samples, and monitoring food freshness.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yuanyuan Qin, Shuda Liu, Shuyun Meng, Dong Liu, Tianyan You
Summary: The study developed a split aptamer-based sandwich-type ratiometric biosensor for the detection of 17 beta-estradiol (E2) using photoelectrochemical and electrochemical assays. The biosensor utilized split aptamer fragments to recognize E2 and trigger a hybridization chain reaction, resulting in the production of double-stranded DNA labeled with CdTe quantum dots. This DNA complex was able to sensitize CdTe quantum dots and generate response signals for E2 detection. The developed biosensor demonstrated high sensitivity and accuracy with two linear ranges and low detection limits.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Siriwan Teepoo, Jongjit Jantra, Khaunnapa Panapong, David Taiwo Ajayi
Summary: A novel immunochromatographic assay based on hyperbranched Au plasmonic blackbodies with a smartphone readout was developed for rapid and sensitive detection of leucomalachite green residues in fish and shrimp products.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Andrea L. Larraga-Urdaz, Borja Moreira-Alvarez, Jorge Ruiz Encinar, Jose M. Costa-Fernandez, Maria Luisa Fernandez-Sanchez
Summary: A major challenge in the 21st century is the development of point-of-care diagnostic tools. In this study, a highly sensitive and simple bioassay using AuNPs and MNAzymes was developed for rapid detection and quantification of miRNA-4739. The proposed strategy shows potential for breast cancer diagnosis.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Xiaohan Zhao, Anyu Wang, Lingzi Zhai, Jiuhe Gao, Sizhe Lyu, Yingshan Jiang, Tian Zhong, Ying Xiao, Xi Yu
Summary: A novel method using polystyrene-coated magnetic nanoparticles for extracting monohydroxy polycyclic aromatic hydrocarbons from urine samples was investigated. The proposed method is simple, sensitive, and efficient, with desirable sensitivity for analyzing low-abundance metabolites in large volumes of complex urine samples.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Quan, Yuqing Zeng, Wenke Zhang, Fengfeng Li, Mengjiao Li, Zhihe Qing, Linlin Wu
Summary: In this study, a visual and reusable biosensor was developed for detecting substrates that are closely associated with human physiological health. The immobilized oxidase showed higher stability and sensitivity under harsh conditions, enabling reliable detection of substrates in complex fluids.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Dongjuan Wang, Xiuqian Ding, Jinling Xie, Juan Wang, Guanhao Li, Xin Zhou
Summary: In this study, a three-in-one sensor was developed for real-time detection of biogenic amines (BAs) with high sensitivity and selectivity. The sensor showed multimodal responses and could be used to fabricate portable devices for on-site non-destructive assessment of food spoilage indicators.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Jinting Meng, Zihao Xu, Shasha Zheng, Hongqun Yang, Tianfu Wang, Hong Wang, Yingwei Zhang
Summary: This study demonstrates the development of a cascade signal amplification system using a multi-pedal DNA walker and strand displacement reactions for the electrochemical detection of miRNA-155. The biosensor exhibited high sensitivity, selectivity, and the potential for clinical applications.
ANALYTICA CHIMICA ACTA
(2024)