Article
Microbiology
Baba Uqab, Ruqeya Nazir, Bashir Ahmad Ganai, Praveen Rahi
Summary: Due to industrialization, the contamination of toxic metals in soils is a major global concern. Cadmium, a highly toxic metal, can enter plant cells and affect their uptake functions. This study examines the response and tolerance of various bacteria to different concentrations of cadmium, and identifies bacteria with cadmium tolerance and sequestration potential. Among the bacteria tested, Klebsiella pneumonia showed the highest tolerance and sequestration potential for cadmium.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ivana Charousova, Miroslava Hlebova, Lukas Hleba, Juraj Medo, Joachim Wink
Summary: A new actinomycete producer of the antibiotic gamma-rubromycin, designated TA 36, was successfully identified as S. iakyrus through morphological, physiological and biochemical characteristics, phylogenetic analysis, and MALDI-TOF MS data. The crude extract of TA 36 showed strong antimicrobial activity against Staphylococcus aureus, and further analysis identified the presence of the promising antitumor and antibacterial antibiotic gamma-rubromycin. This study suggests that S. iakyrus TA 36 is the first-reported source of this unique bioactive secondary metabolite.
Article
Biotechnology & Applied Microbiology
Se-Hyung Kim, Jung-Whan Chon, Hyo-Won Jeong, Kwang-Young Song, Dong-Hyeon Kim, Dongryeoul Bae, Hyunsook Kim, Kun-Ho Seo
Summary: This study compared the accuracy of MALDI-TOF MS, VITEK 2, and 16S rRNA gene sequencing in identifying Enterococcus species. The results showed that MALDI-TOF MS correctly identified all isolates at the species level except for one, while VITEK 2 misidentified ten isolates. However, the phylogenetic trees constructed from both methods showed similar positions for all isolates. The study clearly demonstrated that MALDI-TOF MS is a reliable and rapid tool for identifying Enterococcus species with greater discriminatory power than VITEK 2.
Article
Microbiology
Arkadiusz Jozef Zakrzewski, Urszula Zarzecka, Wioleta Chajecka-Wierzchowska, Anna Zadernowska
Summary: This study aimed to compare the effectiveness of different identification methods for Enterobacterales strains isolated from fresh fish and shrimps. The results showed that conventional identification methods could provide false results, while the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) had the highest genus identification rate.
Article
Environmental Sciences
Samet Kalkan
Summary: This study focused on the marine sediments in the Black Sea and explored the value of bacterial diversity for public health and biotechnology applications. Through the collection of sediment samples and the use of multimodal analytical platforms, 26 different bacterial species were identified, including some newly reported species. These findings contribute to a better understanding of microbial life in the southeastern sediments of the Black Sea.
MARINE POLLUTION BULLETIN
(2022)
Article
Microbiology
Elisabete Alves Cappelli, Magdalena Ksiezarek, Jacqueline Wolf, Meina Neumann-Schaal, Teresa Goncalves Ribeiro, Luisa Peixe
Summary: The genus Corynebacterium is frequently found in the female urinary microbiome, but species-level characterization of Corynebacterium has been underexplored. In this study, eight strains isolated from urine samples were characterized and identified as potentially new Corynebacterium species.
Article
Biotechnology & Applied Microbiology
Eiseul Kim, Eun-Ji Cho, Seung-Min Yang, Mi-Ju Kim, Hae-Yeong Kim
Summary: A study compared culture-dependent and culture-independent methods for identifying microbial community changes during kimchi fermentation. Both methods identified Lactobacillus-related genera, Leuconostoc, and Weissella as predominant microorganisms, with the culture-independent method additionally identifying non-lactic acid bacteria and yeasts. The culture-dependent method could identify species level and predominant species in viable cell communities, while culture-independent method provided a more comprehensive view of the microbial ecology in fermented kimchi.
Article
Immunology
Yingmiao Zhang, Yue Fan, Yu Zhan, Hao Wang, Xun Li, Hui Wang, Tian Feng, Lifeng Shi, Jing Wang, Zhongxin Lu
Summary: This study reports the first case of human infection caused by P. anthophila in China, providing a fundamental resource for further investigation of its virulence factors, antibiotic resistance, host-pathogen interactions, and comparative genomics of the Pantoea genus.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Food Science & Technology
Zhongjia Yu, Marie Joossens, Pieter -Jan Kerkhof, Kurt Houf
Summary: Freezing broiler carcasses can delay spoilage and hinder the growth of food pathogens, but may not be an effective safety hurdle against bacterial contamination. The study found that freezing did not significantly affect the levels of aerobic bacteria on carcasses, and short freezing periods did not result in differences in bacterial loads compared to longer freezing periods. Industrial frozen carcasses showed no presence of Salmonella and Campylobacter, while fresh carcasses had detectable levels both before and after freezing.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Robin Vanstokstraeten, Shari Mackens, Ellen Callewaert, Susanne Blotwijk, Kristof Emmerechts, Florence Crombe, Oriane Soetens, Ingrid Wybo, Kristof Vandoorslaer, Laurence Mostert, Deborah De Geyter, Astrid Muyldermans, Christophe Blockeel, Denis Pierard, Thomas Demuyser
Summary: This study demonstrates the applicability and added value of a high-throughput culturing approach (culturomics) to investigate the endometrial microbiome at a species level. The results show that this method can identify most isolates at the species level and contribute to understanding the composition of the low biomass endometrial microbiome.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Environmental Sciences
Anna Pinar-Mendez, Belen Galofre, Anicet R. Blanch, Cristina Garcia-Aljaro
Summary: Bacterial communities in a full-scale drinking water treatment plant were characterized using MALDI-TOF MS and 16S rRNA metabarcoding methods. Both approaches showed similar trends in diversity, but differences were observed in the detection and classification of taxa. The diversity of bacteria varied in different water treatment processes, and these findings are of importance for understanding the role of bacteria in water treatment and quality.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Food Science & Technology
Jaana Huotari, Irina Tsitko, Kaisu Honkapaa, Hanna-Leena Alakomi
Summary: There is growing interest in using whole Baltic herring for food consumption and this study explored the suitability of ungutted herring for this purpose. The microbiological quality of commercially fished whole and gutted herring was analyzed, and it was found that the bacterial diversity was good and the microbiological quality was acceptable in all the analyzed fish batches.
Article
Microbiology
Anna Werinder, Anna Aspan, Robert Soderlund, Annette Backhans, Marie Sjolund, Bengt Guss, Magdalena Jacobson
Summary: The study evaluated the identification of Streptococcus suis isolates using different sequence analysis methods, revealing the need for species confirmation beyond MALDI-TOF MS. ANI analysis was considered the most accurate classification method among the various approaches used.
JOURNAL OF CLINICAL MICROBIOLOGY
(2021)
Article
Microbiology
Botond Bendeguz Suranyi, Benjamin Zwirzitz, Csilla Mohacsi-Farkas, Tekla Engelhardt, Konrad J. Domig
Summary: In order to enhance agricultural productivity and feed the growing population, irrigation is crucial. However, the use of poorly characterized water sources increases the prevalence of foodborne diseases. Therefore, analyzing microorganisms in irrigation water can help prevent such cases.
Article
Mathematical & Computational Biology
Diego Arnal, Celeste Moya, Luigi Filippelli, Jaume Segura-Garcia, Sergi Maicas
Summary: The aim of this study was to map the spatial distribution of potential dangerous Enterobacteria and identify useful bioremediation isolates from soils in an urban park in Valencia, Spain. The team collected 25 soil samples and isolated 500 microorganisms, using a mobile application to collect information on soil samples with geolocation. They established a combined protocol using MALDI-TOF MS and 16S rDNA sequencing PCR to characterize the isolates. The results were processed using spatial statistical techniques, increasing the number of samples and predicting the quality of soil in urban parks.
Article
Infectious Diseases
S. Nguyen, O. Robineau, M. Titecat, N. Blondiaux, M. Valette, C. Loiez, E. Beltrand, H. Migaud, E. Senneville
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2015)
Article
Infectious Diseases
Marie Titecat, Eric Senneville, Frederic Wallet, Herve Dezeque, Henri Migaud, Rene J. Courcol, Caroline Loiez
SURGICAL INFECTIONS
(2015)
Article
Infectious Diseases
E. Fiaux, M. Titecat, O. Robineau, J. Lora-Tamayo, Y. El Samad, M. Etienne, N. Frebourg, N. Blondiaux, B. Brunschweiler, F. Dujardin, E. Beltrand, C. Loiez, V. Cattoir, J. P. Canarelli, C. Hulet, M. Valette, S. Nguyen, F. Caron, H. Migaud, E. Senneville
BMC INFECTIOUS DISEASES
(2016)
Article
Infectious Diseases
Marie Titecat, Xiaofei Liang, Chul-Jin Lee, Audrey Charlet, Didier Hocquet, Thierry Lambert, Jean-Marie Pages, Rene Courcol, Florent Sebbane, Eric J. Toone, Pei Zhou, Nadine Lemaitre
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2016)
Letter
Microbiology
Marie Titecat, Frederic Wallet, Olivier Robineau, Michel Valette, Henri Migaud, Eric Senneville, Caroline Loiez
JOURNAL OF CLINICAL MICROBIOLOGY
(2017)
Article
Immunology
Catherine Daniel, Marie Titecat, Sabine Poiret, Delphine Cayet, Denise Boutillier, Michel Simonet, Jean-Claude Sirard, Nadine Lemaitre, Florent Sebbane
Article
Multidisciplinary Sciences
Chul-Jin Lee, Xiaofei Liang, Qinglin Wu, Javaria Najeeb, Jinshi Zhao, Ramesh Gopalaswamy, Marie Titecat, Florent Sebbane, Nadine Lemaitre, Eric J. Toone, Pei Zhou
NATURE COMMUNICATIONS
(2016)
Article
Immunology
Adeline Mambie, Frederic Wallet, Laurine Scherman, Sylvie Armand, Christine Vervelle, Karine Faure, Benoit Guery, Marie Titecat, Caroline Loiez
OPEN FORUM INFECTIOUS DISEASES
(2016)
Article
Infectious Diseases
M. Pradier, O. Robineau, A. Boucher, M. Titecat, N. Blondiaux, M. Valette, C. Loiez, E. Beltrand, S. Nguyen, H. Dezeque, H. Migaud, Eric Senneville
Article
Infectious Diseases
M. Pradier, S. Nguyen, O. Robineau, M. Titecat, N. Blondiaux, M. Valette, C. Loiez, E. Beltrand, H. Dezeque, H. Migaud, E. Senneville
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2017)
Article
Microbiology
Nadine Lemaitre, Xiaofei Liang, Javaria Najeeb, Chul-Jin Lee, Marie Titecat, Emmanuelle Leteurtre, Michel Simonet, Eric J. Toone, Pei Zhou, Florent Sebbane
Article
Gastroenterology & Hepatology
Oumaira Rahmouni, Cecile Vignal, Marie Titecat, Benoit Foligne, Benjamin Pariente, Laurent Dubuquoy, Pierre Desreumaux, Christel Neut
Article
Immunology
Angeline Reboul, Nadine Lemaitre, Marie Titecat, Maud Merchez, Gaspard Deloison, Isabelle Ricard, Elizabeth Pradel, Michael Marceau, Florent Sebbane
JOURNAL OF INFECTIOUS DISEASES
(2014)
Article
Biochemistry & Molecular Biology
Fanny George, Marie Titecat, Nicolas Barois, Catherine Daniel, Anne Garat, Gwenael Jan, Benoit Foligne
Summary: Microbial agents show promise in bioremediation, biodecontamination, and toxicity alleviation. Adding Tween(R)80 to a culture of Propionibacterium freudenreichii significantly enhances its ability to remove lead from water.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Letter
Food Science & Technology
Benoit Foligne, Quentin Menetrey, Marie Titecat
COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY
(2022)
