Article
Veterinary Sciences
Alina Hagen, Sabine Niebert, Vivian-Pascal Brandt, Heidrun Holland, Michaela Melzer, Axel Wehrend, Janina Burk
Summary: This study analyzed the functional properties of equine adipose-derived MSC cultured with equine platelet lysate (ePL), and found that ePL not only supported MSC's proliferation and basic characteristics, but also enhanced their cell fitness and pro-angiogenic potency.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Engineering, Biomedical
Nai-Hao Yin, Ian McCarthy, Helen L. Birch
Summary: This study proposes the equine deep digital flexor tendon and its accessory ligament as a model to study the sliding mechanism between sub-tendons, demonstrating significant regional strain differences and greater force transmission from the accessory ligament to the tendon than vice versa. The results suggest that the distribution of load between sub-structures is highly dependent on their morphological relationship, which has important implications for understanding tendon mechanical behavior, injury mechanisms, and rehabilitation.
ACTA BIOMATERIALIA
(2021)
Article
Biochemistry & Molecular Biology
Michael J. Mienaltowski, Mitchell Callahan, Nicole L. Gonzales, Angelique Wong
Summary: This study investigated the effects of vitamin C on the tenogenic properties of equine superficial digital flexor tendon cells. It was found that vitamin C had anti-inflammatory effects, alleviated degenerative pathways, and increased collagen content in tendon cells. However, the cytoprotective effect of vitamin C varied between different types of tendon cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Orthopedics
Othman J. Ali, Anna Ehrle, Eithne J. Comerford, Elizabeth G. Canty-Laird, Ashleigh Mead, Peter D. Clegg, Thomas W. Maddox
Summary: This study aimed to characterize intrafascicular chondroid-like bodies (ICBs) in the equine superficial digital flexor tendon (SDFT) and assess the impact of aging on the presence and distribution of these structures. Aging was found to significantly increase the number, size, and distribution of ICBs in the SDFT of older horses, potentially leading to tendon micro-damage before clinical tendinopathy development.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2021)
Article
Veterinary Sciences
Vivian G. Quam, Nadine N. Altmann, Matthew T. Brokken, Sushmitha S. Durgam
Summary: This study aimed to isolate and characterize tendon-derived cells from DDFT in horses, identifying potential cells in the podotrochlear bursa that may enhance healing of DDFT injuries. Promoting chondrogenic properties in cells administered for intrasynovial tendon regeneration could be beneficial.
BMC VETERINARY RESEARCH
(2021)
Article
Cell & Tissue Engineering
Drew W. Koch, Lauren Schnabel, Ilene M. Ellis, Rowan E. Bates, Alix K. Berglund
Summary: Treating equine bone marrow-derived MSCs with TGF-beta 2 significantly increases the production of paracrine factors and extracellular matrix molecules, which are important for tendon healing, and promotes the migration of equine tenocytes in vitro.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Biotechnology & Applied Microbiology
Subhajit Konar, Scott M. Bolam, Brendan Coleman, Nicola Dalbeth, Sue R. McGlashan, Sophia Leung, Jillian Cornish, Dorit Naot, David S. Musson
Summary: Tendinopathy is characterized by pathological changes in tendon matrix composition, architecture, and stiffness, with inflammation also playing an important role. This study found that substrate stiffness affects tendon-derived cells and macrophages. Tendon-derived cells showed minor responses to substrate stiffness, while macrophages exhibited a more inflammatory phenotype on non-physiological stiffness substrates. These subtle variations in matrix stiffness may contribute to the onset and progression of tendinopathy.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Cell Biology
Sergio Garnica-Galvez, Stefanie H. Korntner, Ioannis Skoufos, Athina Tzora, Nikolaos Diakakis, Nikitas Prassinos, Dimitrios I. Zeugolis
Summary: This study evaluated the physicochemical properties of different concentrations of hyaluronic acid (HA) and their influence on equine adipose-derived stem cell cultures. It found that 10 mg/mL 100 kDa and 500 kDa HAs exhibited the highest negative charge and hydrodynamic radius among the different concentrations and molecular weights. Despite the potential of HA as a macromolecular crowder, it did not outperform carrageenan and Ficoll(R) in accelerating collagen deposition.
Article
Biochemistry & Molecular Biology
Cheng-Chang Lu, Cheng-Jung Ho, Hsuan-Ti Huang, Sung-Yen Lin, Shih-Hsiang Chou, Pei-Hsi Chou, Mei-Ling Ho, Yin-Chun Tien
Summary: Both bone marrow mononuclear cells (BMMNCs) and bone marrow stromal cells (BMSCs) have comparable effects on cell repopulation and interfacial allograft-bone healing after allograft anterior cruciate ligament reconstruction (ACLR). Intra-articular delivery of BM cells has limited benefits on graft cell repopulation and may cause higher inflammation in the early stage, with fibrocartilage formation in the tendon-bone interface.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Orthopedics
Shu-Kun He, Liang-Ju Ning, Xuan Yao, Ruo-Nan Hu, Jing Cui, Yi Zhang, Wei Ding, Jing-Cong Luo, Ting-Wu Qin
Summary: The study successfully promoted the repair of rotator cuff in a rabbit model by using hierarchically demineralized cortical bone (hDCB) coated with stem cell-derived extracellular matrix (hDCB-ECM), showing potential clinical applications.
AMERICAN JOURNAL OF SPORTS MEDICINE
(2021)
Article
Immunology
Lien Gysens, Eva Depuydt, Marco Patruno, Maarten Haspeslagh, Jan H. Spaas, Ann Martens
Summary: This in-vitro study aimed to evaluate the induction of a humoral immune response against equine sarcoid-derived bovine papilloma-virus (BPV)-1 infected fibroblasts using a flow cytometric crossmatch assay. The results showed that antibodies against autologous sarcoid cells were present in some sarcoid-affected horses, supporting the hypothesis that these horses may develop antibodies recognizing tumor-specific antigens. However, equine PBMCs did not seem to contain complete BPV genomes.
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
(2023)
Article
Developmental Biology
Zoe Tasma, Weilin Hou, Tanvi Damani, Kathleen Seddon, Matthew Kang, Yi Ge, David Hanlon, Fiona Hollinshead, Colin L. Hisey, Lawrence W. Chamley
Summary: In this study, MSCs were isolated from equine embryos and used to produce EVs in bioreactors. The CELLine AD 1000 flasks showed higher cell density cultures and more EV production compared to the FiberCell system or conventional culture flasks. These findings will contribute to the future isolation and scalable culture of equine MSCs and their EVs.
Article
Orthopedics
Juan Paredes, Ashley Pekmezian, Nelly Andarawis-Puri
Summary: The study found that the scarless-healing MRL/MpJ tendons have therapeutic potential for improving the structure and function of tendons in traditional healing models. This suggests a promising avenue for future therapeutic development and tissue engineering applications.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2021)
Article
Cell Biology
Ivana Bertovic, Ana Bura, Antonija Jurak Begonja
Summary: Various lines of evidence indicate differences in megakaryopoiesis during development. In this study, megakaryocytes cultured from adult bone marrow and fetal livers were compared, showing that fetal liver-derived MKs produce more proplatelets in vitro, express higher levels of beta 1-tubulin, and exhibit increased internal GPIb beta staining and fluorescence intensity of GPIb beta and VWF. The data suggest that fetal liver MKs undergo cytoplasmic maturation earlier than bone marrow MKs, leading to more efficient proplatelet formation in vitro.
Article
Agriculture, Dairy & Animal Science
Ines Leal Reis, Bruna Lopes, Patricia Sousa, Ana Catarina Sousa, Mariana Branquinho, Ana Rita Caseiro, Silvia Santos Pedrosa, Alexandra Rema, Claudia Oliveira, Beatriz Porto, Luis Atayde, Irina Amorim, Rui Alvites, Jorge Miguel Santos, Ana Colette Mauricio
Summary: This study developed a treatment method that promotes tissue regeneration by injecting equine synovial stem cells and autologous serum into the lesion site. After treatment, all horses underwent a physical rehabilitation program and were monitored with physical and ultrasonographic exams. The results were very promising, supporting the use of equine synovial stem cells and autologous serum in the treatment of tendonitis and desmitis.
Article
Orthopedics
Sushmitha S. Durgam, Allison A. Stewart, Mayandi Sivaguru, Amy J. Wagoner Johnson, Matthew C. Stewart
JOURNAL OF ORTHOPAEDIC RESEARCH
(2016)
Article
Veterinary Sciences
M. L. Byrum, H. C. Pondenis, R. L. Fredrickson, K. L. Wycislo, T. M. Fan
JOURNAL OF VETERINARY INTERNAL MEDICINE
(2016)
Article
Cell & Tissue Engineering
Sushmitha Durgam, Brooke Schuster, Anna Cymerman, Allison Stewart, Matthew Stewart
TISSUE ENGINEERING PART C-METHODS
(2016)
Article
Veterinary Sciences
Alastair T. Kay, Sushmitha Durgam, Matthew Stewart, Stephen Joslyn, David J. Schaeffer, Gavin Horn, Richard Kesler, Peter Chew
VETERINARY SURGERY
(2016)
Article
Cell & Tissue Engineering
Marta Bianchessi, Yuwen Chen, Sushmitha Durgam, Holly Pondenis, Matthew Stewart
STEM CELLS INTERNATIONAL
(2016)
Article
Cell & Tissue Engineering
Sushmitha Durgam, Matthew Stewart
TISSUE ENGINEERING PART B-REVIEWS
(2017)
Article
Multidisciplinary Sciences
Jahnabi Roy, Kathryn L. Wycislo, Holly Pondenis, Timothy M. Fan, Aditi Das
Article
Multidisciplinary Sciences
Matthew Dowling, Jonathan Samuelson, Bahaa Fadl-Alla, Holly C. Pondenis, Mark Byrum, Anne M. Barger, Timothy M. Fan
Article
Orthopedics
Michael Baria, W. Kelton Vasileff, Meghan Miller, James Borchers, David C. Flanigan, Sushmitha S. Durgam
AMERICAN JOURNAL OF SPORTS MEDICINE
(2019)
Article
Oncology
Lisa J. Schlein, Bahaa Fadl-Alla, Holly C. Pondenis, Stephane Lezmi, Charles G. Eberhart, Amy K. LeBlanc, Peter J. Dickinson, Paul J. Hergenrother, Timothy M. Fan
FRONTIERS IN ONCOLOGY
(2019)
Article
Veterinary Sciences
R. E. Ellerbrock, I. F. Canisso, P. J. Roady, A. Litsky, S. Durgam, G. Podico, Z. Li, F. S. Lima
EQUINE VETERINARY JOURNAL
(2020)
Article
Veterinary Sciences
Joseph W. Lozier, Andrew J. Niehaus, C. Austin Hinds, Sushmitha S. Durgam, Stephen C. Jones, Jeffrey Lakritz
AMERICAN JOURNAL OF VETERINARY RESEARCH
(2019)
Article
Cell & Tissue Engineering
Sushmitha S. Durgam, Nadine N. Altmann, Haley E. Coughlin, Audrey Rollins, Laura D. Hostnik
STEM CELLS INTERNATIONAL
(2019)
Article
Veterinary Sciences
Vivian G. Quam, Nadine N. Altmann, Matthew T. Brokken, Sushmitha S. Durgam
Summary: This study aimed to isolate and characterize tendon-derived cells from DDFT in horses, identifying potential cells in the podotrochlear bursa that may enhance healing of DDFT injuries. Promoting chondrogenic properties in cells administered for intrasynovial tendon regeneration could be beneficial.
BMC VETERINARY RESEARCH
(2021)