Article
Medicine, General & Internal
Skye R. MacGregor, Donald P. McManus, Haran Sivakumaran, Thomas G. Egwang, Moses Adriko, Pengfei Cai, Catherine A. Gordon, Mary G. Duke, Juliet D. French, Natasha Collinson, Remigio M. Olveda, Gunter Hartel, Carlos Graeff-Teixeira, Malcolm K. Jones, Hong You
Summary: The study developed a CRISPR-based diagnostic platform called SHERLOCK for the detection of Schistosoma japonicum and S. mansoni. This platform has the characteristics of high sensitivity, specificity, cost-effectiveness, and portability. By testing animal and human samples, it demonstrated the potential of this platform for rapid and accurate diagnosis and surveillance of parasitic neglected tropical diseases.
Article
Chemistry, Analytical
Xinfa Cai, Meijuan Liang, Fei Ma, Sherif Ramzy Mohamed, Amira Abdel Goda, Dawood H. Dawood, Li Yu, Peiwu Li
Summary: A direct competitive nanozyme-linked immunosorbent assay based on MnO2 nanosheets as a nanozyme label was developed for highly sensitive determination of fumonisin B-1. The method showed a wide linear range, low detection limit, high accuracy, and good consistency with the reference method for screening FB1 in cereals.
ANALYTICAL METHODS
(2021)
Review
Immunology
Estelle M. Mewamba, Oscar A. Nyangiri, Harry A. Noyes, Moses Egesa, Enock Matovu, Gustave Simo
Summary: This review focuses on how genetic factors may influence parasite load, hepatic fibrosis and portal hypertension in schistosomiasis. Studies have shown a potential role for the T(h)17 pathway genes in controlling infection intensity and pathology. Future studies should prioritize investigating the effects of variants in this pathway on regulating these phenotypes.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Medical Laboratory Technology
E. Karakece, M. Koroglu, Huseyin A. Terzi, O. Aydemir, U. Erkorkmaz, Ferhat G. Aslan, M. Altindis
Summary: This study compared the role of the enzyme linked fluorescent assay (ELFA) method with enzyme-linked immunosorbent assay (ELISA) and chemiluminescent microplate immunoassay (CMIA) methods in the diagnosis of HIV infection. It was found that ELFA method had a lower false positive rate and high agreement with confirmation tests, suggesting a potential role in the HIV diagnostic algorithm.
CLINICAL LABORATORY
(2022)
Article
Infectious Diseases
Pengfei Cai, Yi Mu, Kosala G. Weerakoon, Remigio M. Olveda, Allen G. Ross, Donald P. McManus
Summary: The POC-CCA test showed sensitivity/specificity values of 63.3%/93.3% for detecting individuals infected with S. japonicum with high egg burden, but its performance was limited in diagnosing infections with low egg burden. Therefore, caution should be exercised when applying this test in populations with low intensity S. japonicum infections.
INFECTIOUS DISEASES OF POVERTY
(2021)
Article
Microbiology
Ching-Chi Lee, Jen-Chieh Lee, Chun-Wei Chiu, Pei-Jane Tsai, Wen-Chien Ko, Yuan-Pin Hung
Summary: The importance of detecting toxins or toxin genes when diagnosing Clostridioides difficile infections (CDIs) or predicting the severity and outcomes of CDI has been emphasized. Although the usefulness of stool cultures in CDI diagnoses is limited, the absence of C. difficile growth in stool may predict successful CDI therapy.
MICROBIOLOGY SPECTRUM
(2021)
Article
Nanoscience & Nanotechnology
Wenhao Li, Tiange Gao, Chenxi Lou, Haifang Wang, Yuanfang Liu, Aoneng Cao
Summary: This study presents a novel artificial antibody called Goldbody, which is based on gold nanoparticles and exhibits comparable specificity and improved stability compared to monoclonal antibodies. The Goldbody was successfully utilized in competitive LFIA and ELISA assays for the detection of lysozyme, demonstrating its potential as a substitute for monoclonal antibodies in immunoassays.
ACS APPLIED NANO MATERIALS
(2022)
Article
Immunology
Yi Mu, Donald P. McManus, Catherine A. Gordon, Hong You, Allen G. Ross, Remigio M. Olveda, Pengfei Cai
Summary: A novel gold immunochromatographic assay (GICA) was developed for the detection of Schistosoma japonicum infection and showed high sensitivity and specificity. It can serve as a rapid, easy-to-use, and field-friendly diagnostic tool for on-site surveillance/screening of S. japonicum infection.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Chemistry, Analytical
Xue Liu, Xiuzhai Chen, Xu Zhu, Qing Lin, Xi Pan, Xiaolei Tan, Yongfeng Guo, Jun Qiu, Song Fang
Summary: In this study, ELISA and TRFIA methods were established for the detection and monitoring of quinclorac residue in the environment. The results of both methods were consistent with those of the referenced UPLC-MS/MS method, and they showed good detection performance that met the requirements of quinclorac quantitative determination.
Article
Medical Laboratory Technology
Ying Xu, Chenguang Hu, Jiayu Liu, Haoran Guo, Jingkai Gao, Chi Wang, Chengbin Wang
Summary: Synovial fluid lactoferrin (LTF) and S100 calcium-binding protein A8 (S100A8) have been considered as potential biomarkers for the diagnosis of periprosthetic joint infection (PJI). In this study, we developed chemiluminescent immunoassays (CLIA) for their automated detection and evaluated their analytical performance. The established CLIA methods showed excellent diagnostic efficiency for PJI and have the advantages of automation, high throughput, and low cost, making them promising for clinical applications.
CLINICA CHIMICA ACTA
(2023)
Article
Chemistry, Medicinal
Michalis Platis, Dimitrios Vlachakis, Ahmed Ibrahim Foudah, Magdy Mohamed Muharram, Mohamed Hamed Alqarni, Anastassios C. Papageorgiou, Nikolaos E. Labrou
Summary: The study demonstrated that CB3GA is a potent inhibitor of SjGST, providing insights for potential drug design efforts targeting SjGST.
MEDICINAL CHEMISTRY
(2021)
Article
Immunology
Esther Gomez-Vicente, Rafael Garcia, Elizabeth Calatrava, Maria Jose Olivares Duran, Juan Francisco Gutierrez-Bautista, Javier Rodriguez-Granger, Fernando Cobo, Jose Maria Navarro Mari, Antonio Sampedro-Martinez
Summary: This study compared the agreement, sensitivity, and specificity of two different methods for detecting West Nile virus (WNV) infection. The results showed that the WNV VIRCLIA IgM and IgG monotest offers accurate detection of WNV in serum and cerebrospinal fluid (CSF) specimens.
Article
Chemistry, Analytical
Fatema Kaladari, Mahmoud El-Maghrabey, Naoya Kishikawa, Naotaka Kuroda
Summary: A sensitive and stable signal multiplied quinone-linked immunosorbent assay (Multi-QuLISA) was developed using a compound called Bio8mer-NQ as a signal-generating label. This compound showed better stability than the commonly used enzyme, biotin-HRP, and demonstrated higher sensitivity in both colorimetric and chemiluminescence assays.
Article
Chemistry, Analytical
Mahmoud El-Maghrabey, Yudai Sato, Fatema Kaladari, Naoya Kishikawa, Naotaka Kuroda
Summary: Enzyme-labeled immunoassays are widely used for trace analysis of clinically significant compounds. However, utilizing enzymes for labeling antibodies can lead to problems such as low reproducibility and inhibition of antigen-antibody reaction. In this study, we used quinones as stable non-enzymatic tags and developed a simple, stable, sensitive, and cost-effective approach for labeling immunoassays. We successfully developed a quinone-linked immunosorbent assay (QuLISA) for detecting food allergens, which showed good linearity and high sensitivity.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Izumi Morita, Yuki Kiguchi, Hiroyuki Oyama, Atsuko Takeuchi, Chisato Tode, Rie Tanaka, Jun Ogata, Ruri Kikura-Hanajiri, Norihiro Kobayashi
Summary: A novel ELISA method was developed using a higher affinity monoclonal antibody against TBS/Psi instead of Psi to accurately identify hallucinogenic mushrooms, achieving the desired sensitivity and overcoming previous limitations.
ANALYTICAL METHODS
(2021)
Article
Engineering, Environmental
Dingwen Shi, Hongsen Zhang, Haining Zhang, Ling Li, Suping Li, Yanbing Zhao, Chuansheng Zheng, Guangjun Nie, Xiangliang Yang
Summary: In this study, TF-Nanogels, formed by entrapping tTF-pHLIPs into PIB nanogels, exhibited a synergistic effect, enhancing the anti-tumor efficacy of trans-arterial embolization.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Chemistry, Multidisciplinary
Xiaoqiang Zhu, Sihan Chen, Xiuwen Hu, Lijun Zhao, Yiqian Wang, Jinzhao Huang, Jiawen Chen, Yuzhi Qiu, Xuefei Zhang, Mengdie Wang, Xiangliang Yang, Yan Zhang, Yanhong Zhu
Summary: Researchers have developed a bacteria-based cancer immunotherapy using engineered Escherichia coli and lanthanide upconversion nanoparticles. By combining optogenetic tools with the natural behavior of certain bacteria, this novel therapy achieved significant tumor regression with no side effects.
ADVANCED MATERIALS
(2023)
Article
Chemistry, Physical
Yiming Liu, Dingwen Shi, Yanqiao Ren, Ling Li, Yanbing Zhao, Chuansheng Zheng, Xiangliang Yang
Summary: In this study, gold nanoparticles modified temperature sensitive polymer was successfully used to encapsulate doxorubicin and developed into gold nanomedicines. The results showed that these gold nanomedicines exhibited excellent antitumor efficacy and improved the tumor immunosuppressive microenvironment.
Article
Chemistry, Multidisciplinary
Qingqing Zhang, Dingwen Shi, Mengqin Guo, Hao Zhao, Yanbing Zhao, Xiangliang Yang
Summary: Pyroptosis of tumor cells was induced by biGC@PNA with a precisely stoichiometric ratio of Au(I) ion/Au(0) atom through its radiofrequency (RF)-heating effect. The activated pyroptosis resulted in a robust immunogenic cell death (ICD) effect, enhancing the antitumor immune responses and suppressing tumor metastasis and relapse. The noninvasive RF field provides a promising strategy for improving cancer immunotherapy.
Article
Chemistry, Multidisciplinary
Wen Qin, Jinzhao Huang, Chunsheng Yang, Quer Yue, Shizhen Chen, Mengdie Wang, Shangbang Gao, Xin Zhou, Xiangliang Yang, Yan Zhang
Summary: This study presents a novel activatable ferrous nanotheranostics for spatial-temporal control over antitumor ferroptosis responses. The nanotherapeutics can be guided by photoacoustic and magnetic resonance imaging (MRI), and it can effectively induce tumor cell death through photothermal therapy and Fenton reaction. This treatment not only suppresses tumor growth, but also promotes immune response against the tumor.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Engineering, Environmental
Xiaoqiong Zhang, Zhaohan Wei, Ziqiao Ding, Weilin Lv, Jianye Li, Xin Li, Haojie Liu, Panli Yu, Xiangliang Yang, Lu Gan
Summary: Mannose-engineered macrophage-derived microparticles can efficiently repolarize M2-like TAMs enriched with Doxil, improving the immunotherapy and increasing tumor accumulation and penetration of Doxil.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Chemistry, Multidisciplinary
Shinan Geng, Mengqin Guo, Guiting Zhan, Dingwen Shi, Liyun Shi, Lu Gan, Yanbing Zhao, Xiangliang Yang
Summary: A NIR light-triggered dePEGylation/ligand-presenting strategy based on thermal decomposition of azo bonds is developed in this study. It overcomes the PEG dilemma and improves tumor targeting and drug delivery by enhancing cellular uptake and tumor accumulation.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Chemistry, Multidisciplinary
Chong Wang, Qiang Wang, Huimin Wang, Zheng Li, Jitang Chen, Zhijie Zhang, Haowen Zeng, Ximiao Yu, Xiaoquan Yang, Xiangliang Yang, Zifu Li
Summary: This study developed a small molecular prodrug-based nanomedicine with high drug-loading efficiency and tumor selectivity for the treatment of solid tumors, especially triple negative breast cancers. The nanomedicine actively targeted TNBC 4T1 tumor tissues and achieved reduction-responsive drug release. It significantly suppressed tumor growth and eliminated cancer stem cells by disrupting the tumor microenvironment and consuming intracellular glutathione. This research provides valuable insights for the rational design of prodrug nanomedicines for the treatment of stroma- and CSCs-rich solid malignancies.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Chemistry, Physical
Chong Wang, Huimin Wang, Hai Yang, Chen Xu, Qiang Wang, Zheng Li, Zhijie Zhang, Jiankun Guan, Ximiao Yu, Xiaoquan Yang, Xiangliang Yang, Zifu Li
Summary: Researchers designed a nanomedicine that specifically targets cancer-associated fibroblasts (CAFs) in triple-negative breast cancer (TNBC) by conjugating doxorubicin-loaded hydroxyethyl starch-IR780 nanoparticles with a CREKA peptide. The nanomedicine effectively targeted CAFs and generated hyperthermia upon light irradiation, leading to a reduction in CAFs through chemo- and photothermal-therapies. By modulating the tumor mechanical microenvironment (TMME) and eliminating cancer stem cells (CSCs), the growth of the 4T1 tumor was significantly inhibited. This strategy provides a novel therapeutic treatment for desmoplastic solid tumors.
Article
Chemistry, Analytical
Jinzhao Huang, Xuefei Zhang, Shiyu Li, Fei Qu, Biao Huang, Ran Cui, Yijing Liu, Wenbo Hu, Xiangliang Yang, Yan Zhang
Summary: We report a synthesis method for dye-sensitized fluorescent probes, which enhance the fluorescence performance of lanthanide nanoparticles at high doping concentration of Nd3+ by adding indocyanine green (ICG), enabling activatable second near-infrared (NIR-II) fluorescent imaging.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Yuxuan Xiong, Wei Wang, Qingyuan Deng, Zhijie Zhang, Qiang Wang, Zhengtao Yong, Chaoyang Sun, Xiangliang Yang, Zifu Li
Summary: Various PARPi have been approved for the treatment of breast cancers, but they are only effective for HR-deficient breast cancers. The abnormal micro-environment of breast cancers hinders drug delivery. This study used mesoporous polydopamine to deliver PARPi and a DNA-damaging drug, and demonstrated that local mild photothermal therapy could inhibit the HR repair pathway and repress cancer-associated fibroblasts, thereby enhancing the antitumor effect and improving drug delivery.
Article
Pharmacology & Pharmacy
Qiang Wang, Shiyou Li, Chen Xu, Ao Hua, Chong Wang, Yuxuan Xiong, Qingyuan Deng, Xiang Chen, Tian Yang, Jiangling Wan, Ze-yang Ding, Bi-xiang Zhang, Xiangliang Yang, Zifu Li
Summary: In this study, a novel derivative of lonidamine (LND) called HYL001 was found to selectively and potently inhibit cancer stem cells (CSCs) by targeting mitochondria, with significantly lower IC50 values compared to LND. Mechanistically, HYL001 downregulates glutaminase expression to block glutamine metabolism, leading to apoptotic cell death. HYL001 also displays significant antitumor activity in vivo and represses CSCs in fresh tumor tissues from liver cancer patients.
PHARMACOLOGICAL RESEARCH
(2023)
Article
Multidisciplinary Sciences
Zheng Li, Yabo Zhu, Haowen Zeng, Chong Wang, Chen Xu, Qiang Wang, Huimin Wang, Shiyou Li, Jitang Chen, Chen Xiao, Xiangliang Yang, Zifu Li
Summary: The authors designed a series of nanogels with different stiffness and investigated how the mechanical properties of the nanogels can overcome the limitations of RES. Stiff nanogels were able to block uptake capacity of macrophages and temporarily block RES, while soft nanogels efficiently delivered drugs. Combining the advantages of stiff and soft nanogels led to the optimum tumor inhibition effect.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Physical
Xi Tan, Hong Zhou, Chenhui Wang, Xuhan Liu, Xiangliang Yang, Wei Liu
Summary: Combinational chemo-immunotherapy using RNA interference (RNAi) to target PD-L1 has been developed to enhance antitumor immune response. A GSH-responsive camptothecin prodrug-based micellar nanoparticle was designed for the co-delivery of siPD-L1 and CPT, showing high loading efficiency and controlled drug release. This study provides a perspective on the design of co-delivery nanoparticles for antitumor chemo-immunotherapy.
Article
Chemistry, Physical
Yiming Liu, Dingwen Shi, Yanqiao Ren, Ling Li, Yanbing Zhao, Chuansheng Zheng, Xiangliang Yang
Summary: In this study, a temperature sensitive polymer modified with gold nanoparticles was used to encapsulate doxorubicin, resulting in gold nanomedicines with improved antitumor efficacy and enhanced post-operative tumor immunosuppressive microenvironment.
