Article
Multidisciplinary Sciences
Xiaolan Liu, Xufeng Cen, Ronghai Wu, Ziyan Chen, Yanqi Xie, Fengqi Wang, Bing Shan, Linghui Zeng, Jichun Zhou, Bojian Xie, Yangjun Cai, Jinyan Huang, Yingjiqiong Liang, Youqian Wu, Chao Zhang, Dongrui Wang, Hongguang Xia
Summary: This study reveals that cisplatin enhances the anti-tumor effect of PD-L1 blockade and upregulates ARIH1 expression, promoting T cell infiltration and sensitizing tumors to immune checkpoint blockade. ARIH1 mediates activation of the STING pathway by ubiquitination and degradation of DNA-PKcs, which is a mechanism of tumor resistance to ICB. Activating ARIH1 is an effective strategy to improve the efficacy of cancer immunotherapy.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Yan Zhang, Xiaodong Lai, Siqing Yang, Huan Ren, Jingya Yuan, Huanchun Jin, Chengchen Shi, Zhibing Lai, Gengshou Xia
Summary: The SlCHIP gene in tomato plays a critical role in heat stress responses by targeting degradation of misfolded proteins. Silencing of SlCHIP reduces heat tolerance in plants, while its overexpression restores heat tolerance to wild type levels. This suggests that SlCHIP is important in regulating plant responses to high temperature stress.
SCIENTIFIC REPORTS
(2021)
Review
Immunology
Min Zou, Qi-Shan Zeng, Jiao Nie, Jia-Hui Yang, Zhen-Yi Luo, Hua-Tian Gan
Summary: Inflammatory bowel disease (IBD) is a complex disorder involving genetic susceptibility, gut microbiota imbalance, mucosal immune disorder, and environmental factors. Recent studies have shown associations between ubiquitination and deubiquitination in the pathogenesis of IBD, highlighting the importance of these post-translational modifications in inflammation development and potential targeted therapeutics for IBD.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Jing Song, Warren Anderson, Alex Hu, Kazushige Obata-Ninomiya, Steven F. Ziegler, David J. Rawlings, Jane H. Buckner
Summary: This study utilized gene editing to knock out the CBLB gene in human CD4+ T cells, revealing that CBLB knockout resulted in hyperproliferation and excessive IL-2 production in CD4+ T cells. The knockout cells showed resistance to Treg suppression and were able to overcome Treg inhibition by producing high levels of cytokines, promoting the proliferation and activation of T effector cells.
JOURNAL OF IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Isshin Shiiba, Keisuke Takeda, Shun Nagashima, Naoki Ito, Takeshi Tokuyama, Shun-Ichi Yamashita, Tomotake Kanki, Toru Komatsu, Yasuteru Urano, Yuuta Fujikawa, Ryoko Inatome, Shigeru Yanagi
Summary: MITOL regulates Parkin-mediated cell death by promoting ubiquitination of Parkin at lysine 220 residue, leading to its proteasomal degradation and controlling mitophagy. Deletion of MITOL results in accumulation of active phosphorylated Parkin in the ER, causing FKBP38 degradation and increased cell death.
Review
Cell Biology
Lei Yuan, Peiyao Li, Qian Zheng, Hui Wang, Hui Xiao
Summary: Ubiquitination and deubiquitination play important roles in apoptosis and apoptotic cell clearance, and are crucial for normal development and immune diseases.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Ying Wang, Jun Liu, Chizuru Akatsu, Runyun Zhang, Hai Zhang, Han Zhu, Kangwei Liu, Han-Ying Zhu, Qing Min, Xin Meng, Chaoqun Cui, Yue Tang, Meiping Yu, Yaxuan Li, Xiaoqian Feng, Hao Wei, Zichao Wen, Sihan Ji, Martin G. Weigert, Takeshi Tsubata, Ji-Yang Wang
Summary: BCR stimulation induces the upregulation of LAPTM5 expression, which triggers apoptosis of immature B cells through two pathways. LAPTM5 mediates BCR internalization and degradation of WWP2, leading to PTEN accumulation, AKT suppression, increased FOXO1 expression, and upregulation of cell cycle inhibitor p27Kip1 and proapoptotic molecule BIM, ultimately contributing to immature B cell apoptosis and B cell tolerance.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Ali Inayat Mallano, Zaib-un Nisa, Binish Khaliq, Naila Ali, Qurban Ali, Chen Chao, Zhu Yanming
Summary: This study isolated and characterized the GsPUB8 protein gene from wild soybean to investigate its response to abiotic stress. The GsPUB8 protein showed higher expression in roots under saline and drought stress, and was found to contain a U-box domain.
SCIENTIFIC REPORTS
(2022)
Article
Oncology
Laura Kristin Esser, Vittorio Branchi, Farhad Shakeri, Adrian Georg Simon, Carsten Stephan, Glen Kristiansen, Andreas Buness, Hubert Schorle, Marieta Ioana Toma
Summary: This study investigated the effects of PARK2 overexpression in ccRCC cell lines and found that it can suppress tumor invasion and migration by regulating the expression of CKS2. It also revealed that high CKS2 levels are associated with high tumor grading and lower patient survival, suggesting CKS2 as a potential biomarker and therapeutic target for ccRCC.
INTERNATIONAL JOURNAL OF ONCOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Orsolya Bilkei-Gorzo, Tiaan Heunis, Jose Luis Marin-Rubio, Francesca Romana Cianfanelli, Benjamin Bernard Armando Raymond, Joseph Inns, Daniela Fabrikova, Julien Peltier, Fiona Oakley, Ralf Schmid, Anetta Hartlova, Matthias Trost
Summary: This study reveals the importance of phagosomal ubiquitylation and the E3 ubiquitin ligase RNF115 in regulating innate immune functions during bacterial infections.
Article
Chemistry, Medicinal
Adam G. Bond, Conner Craigon, Kwok-Ho Chan, Andrea Testa, Athanasios Karapetsas, Rotimi Fasimoye, Thomas Macartney, J. Julian Blow, Dario R. Alessi, Alessio Ciulli
Summary: This study describes the design and development of a new protein degradation system utilizing a variant of the Brd4 bromodomain as a degradation tag. The system effectively degrades BromoTagged proteins in a fast, selective manner, showing favorable pharmacokinetic profile in mice. This system expands the arsenal of chemical genetic degradation tools for manipulating protein levels and exploring therapeutic potential in cells and in vivo.
JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Cell Biology
Martin P. Schwalm, Lena M. Berger, Maximilian N. Meuter, James D. Vasta, Cesear R. Corona, Sandra Roehm, Benedict-Tilman Berger, Frederic Farges, Sebastian M. Beinert, Franziska Preuss, Viktoria Morasch, Vladimir V. Rogov, Sebastian Mathea, Krishna Saxena, Matthew B. Robers, Susanne Mueller, Stefan Knapp
Summary: E3 ligases play a crucial role in regulating protein homeostasis by recruiting substrate proteins to the proteasomal degradation machinery. Recent research has focused on the Baculovirus IAP Repeat (BIR) family of E3 ligases, which contain a structurally conserved but diverse protein interaction domain. The Inhibitors of Apoptosis (IAP) family, which typically have three BIR domains, are promising drug targets. However, there is currently a lack of assay tools to evaluate the selectivity of inhibitors in this target area.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Rui Zhang, Shaoqing Shi
Summary: HECT-type E3 ubiquitin ligases play a vital role in controlling protein function and stability, and members of the NEDD4 family have critical roles in dysregulation of autophagy in cancer cells. This review focuses on the role of NEDD4 E3 ligases in defective autophagy in cancer cells, discussing their function, substrates, and signaling pathways, providing a basis for cancer treatment through modulation of these ligases.
MOLECULAR MEDICINE
(2023)
Review
Immunology
Haoran Cui, Yaxian Zhang, Leiliang Zhang
Summary: Poxviruses have evolved various mechanisms to evade innate immunity, some of which involve poxvirus-encoded E3 ubiquitin ligases and adaptor proteins. These proteins can be categorized into five groups based on their functional domains and ubiquitin transfer mechanisms. Most known substrates of poxvirus E3 ubiquitin ligases are components of the innate immune system. Current research progress provides mechanistic insights into the interaction between these viruses and their hosts.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Oncology
Jia-xin Chen, Da Xu, Jian-wei Cao, Li Zuo, Zhi-tao Han, Yi-jun Tian, Chuan-min Chu, Wang Zhou, Xiu-wu Pan, Xin-gang Cui
Summary: This study demonstrated the crucial role of TRIM47 in promoting malignant biological behavior in renal cell carcinoma, by interacting with P53 protein to increase its ubiquitination and degradation; The TRIM47-P53 axis might be a potential therapeutic target for RCC progression.
CANCER CELL INTERNATIONAL
(2021)
Article
Gastroenterology & Hepatology
Yuto Shiode, Takahiro Kodama, Satoshi Shigeno, Kazuhiro Murai, Satoshi Tanaka, Justin Y. Newberg, Jumpei Kondo, Shogo Kobayashi, Ryoko Yamada, Hayato Hikita, Ryotaro Sakamori, Hiroshi Suemizu, Tomohide Tatsumi, Hidetoshi Eguchi, Nancy A. Jenkins, Neal G. Copeland, Tetsuo Takehara
Summary: The loss of TRAF3 promotes the development of ICC through NIK-mediated cell transdifferentiation, suggesting the potential of the TRAF3-NIK axis as a therapeutic target for ICC.
Article
Gastroenterology & Hepatology
Ryo Sugio, Tsutomu Nishida, Kengo Matsumoto, Kosuke Kawamura, Miyoung Park, Tomoya Hamabe, Kana Hosokawa, Shinji Kuriki, Li-sa Chang, Yoshifumi Fujii, Naoto Osugi, Aya Sugimoto, Kaori Mukai, Dai Nakamatsu, Shiro Hayashi, Masashi Yamamoto, Sachiko Nakajima, Koji Fukui, Masami Inada
Summary: This study retrospectively investigated the risk factors for malignant transformation in pancreatic cyst patients and found that cyst diameter, main pancreatic duct diameter, presence of multilocular cysts, and inconsistent pancreatic duct caliber were significant predictive factors. Stratification based on risk factors may help predict the development of significant changes in pancreatic cyst patients.
DIGESTIVE DISEASES AND SCIENCES
(2023)
Article
Oncology
Kaori Mukai, Tsutomu Nishida, Kengo Matsumoto, Takao Kitanaka, Kana Hosokawa, Naohiro Sakamoto, Satoru Okabe, Yoshifumi Fujii, Naoto Osugi, Aya Sugimoto, Dai Nakamatsu, Masashi Yamamoto, Koji Fukui
Summary: This study aimed to explore predictive markers of oxaliplatin-induced hepatic sinusoidal obstruction syndrome (SOS). The study found that an increase in the FIB-4 index and spleen size can be significant predictive markers for collateral vessel development in patients undergoing oxaliplatin-based chemotherapy.
INTERNATIONAL JOURNAL OF CLINICAL ONCOLOGY
(2023)
Article
Surgery
Yoshiki Tsujii, Yoshito Hayashi, Ryu Ishihara, Shinjiro Yamaguchi, Masashi Yamamoto, Takuya Inoue, Kengo Nagai, Hideharu Ogiyama, Takuya Yamada, Masanori Nakahara, Takashi Kizu, Takashi Kanesaka, Noriko Matsuura, Takashi Ohta, Dai Nakamatsu, Shunsuke Yoshii, Shinichiro Shinzaki, Tsutomu Nishida, Hideki Iijima, Tetsuo Takehara
Summary: The study evaluates the validity of a diagnostic strategy combining conventional endoscopy (CE) and endoscopic ultrasonography (EUS) and determines the additional value of EUS for EGC suspected of submucosal (SM) invasion. The results show that the combination of CE and EUS has a higher diagnostic accuracy compared to CE alone, particularly in differentiating lesion types and low-confidence CE-SM2 lesions.
SURGICAL ENDOSCOPY AND OTHER INTERVENTIONAL TECHNIQUES
(2023)
Article
Oncology
Tatsuya Sakamoto, Tsutomu Nishida, Dai Nakamatsu, Asuka Watanabe, Takao Kitanaka, Yutaro Minoura, Kana Hosokawa, Satoru Okabe, Naohiro Sakamoto, Yoshifumi Fujii, Naoto Osugi, Aya Sugimoto, Kengo Matsumoto, Shiro Hayashi, Sachiko Nakajima, Masashi Yamamoto
Summary: This study aimed to investigate the effect of antithrombotic drugs (ATs) on colorectal cancer (CRC) screening using the fecal immunochemical test (FIT). The results showed that multiple AT use had a lower impact on the positive predictive value (PPV) of FIT, while warfarin use may have an impact on detecting invasive CRC.
ASIA-PACIFIC JOURNAL OF CLINICAL ONCOLOGY
(2023)
Article
Radiology, Nuclear Medicine & Medical Imaging
Koji Nagaike, Shiro Hayashi, Takayuki Yakushijin, Masashi Yamamoto, Tetsuya Sumiyoshi, Shinjiro Yamaguchi, Yuzuru Tamaru, Takuya Yamada, Hidetaka Tsumura, Yousuke Nakai, Hisashi Doyama, Iruru Maetani, Tadayuki Takagi, Satoshi Asai, Kosuke Matsubara, Mamoru Takenaka, Makoto Hosono, Tsutomu Nishida
Summary: This study aims to examine the factors contributing to radiation exposure exceeding the diagnostic reference level (DRL) for transnasal ileus tube placement. The authors analyzed data from the REX-GI study and found that longer procedure time and tube insertion length were associated with DRL exceedance.
BRITISH JOURNAL OF RADIOLOGY
(2023)
Article
Gastroenterology & Hepatology
Shinji Kuriki, Tsutomu Nishida, Li-sa Chang, Kana Hosokawa, Yoshifumi Fujii, Naoto Osugi, Dai Nakamatsu, Kengo Matsumoto, Masashi Yamamoto, Osamu Morimura, Kinya Abe, Yukiyoshi Okauchi, Hiromi Iwahashi, Masami Inada
Summary: This study investigated the gastrointestinal symptoms in Japanese COVID-19 patients and found that 36% of the patients had GI symptoms, with diarrhea being the most common. However, GI symptoms were not associated with the severity of COVID-19.
SCANDINAVIAN JOURNAL OF GASTROENTEROLOGY
(2023)
Article
Multidisciplinary Sciences
Kenji Ikezawa, Shiro Hayashi, Mamoru Takenaka, Takayuki Yakushijin, Koji Nagaike, Ryoji Takada, Takuo Yamai, Kengo Matsumoto, Masashi Yamamoto, Shunsuke Omoto, Kosuke Minaga, Shuji Ishii, Takeshi Shimizu, Kengo Nagai, Makoto Hosono, Tsutomu Nishida
Summary: This study examined occupational radiation exposure to the lens of the eyes during ERCP. Data was collected and radiation exposure was measured for both patients and medical staff. The study found significant correlation between eye dosimeter measurements and patients' radiation exposure, as well as the efficacy of lead glass in reducing radiation exposure. The findings provide valuable information for estimating occupational exposure to the lens of the eyes during ERCP.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Ayako Nakada, Ryota Niikura, Keita Otani, Yusuke Kurose, Yoshito Hayashi, Kazuya Kitamura, Hiroyoshi Nakanishi, Seiji Kawano, Testuya Honda, Kenkei Hasatani, Tetsuya Sumiyoshi, Tsutomu Nishida, Atsuo Yamada, Tomonori Aoki, Tatsuya Harada, Takashi Kawai, Mitsuhiro Fujishiro
Summary: The use of computer-aided detection models in wireless capsule endoscopy (WCE) for diagnosing lesions is a popular endoscopic solution. A revised artificial intelligence (AI) model, RetinaNet, was trained using data from 1234 patients and achieved high AUC values and accuracies for diagnosing erosions and ulcers, vascular lesions, and tumors in WCE images.
Article
Biochemistry & Molecular Biology
Koichi Morishita, Jumpei Kondo, Daisuke Sakon, Ayumu Hayashibara, Ikumi Tamura, Kayoko Shimizu, Shinji Takamatsu, Kohei Murata, Yoshihiro Kamada, Eiji Miyoshi
Summary: This study investigates whether prohaptoglobin (proHp) can serve as a biomarker for colorectal cancer (CRC) and explores its biological functions. The results show that serum proHp is correlated with the clinical stage and prognosis of CRC. Overexpressing proHp in CRC cells induces epithelial-mesenchymal transition-like changes and promotes cell migration.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Oncology
Shota Shimizu, Jumpei Kondo, Kunishige Onuma, Roberto Coppo, Kasumi Ota, Mayumi Kamada, Yohei Harada, Yoshihisa Tanaka, Mai Adachi Nakazawa, Yoshinori Tamada, Yasushi Okuno, Kenji Kawada, Kazutaka Obama, Robert J. Coffey, Yoshiyuki Fujiwara, Masahiro Inoue
Summary: The role of BMP in colorectal cancer is controversial and can act as a tumor promoter or suppressor. This study found that CRC cells reside in a BMP-rich environment, and inhibiting BMP suppresses the growth of CRC cells by inducing LRIG1 expression and decreasing epidermal growth factor receptor. Simultaneous inhibition of BMP and MEK shows cooperative growth inhibition in CRC cells, suggesting a potential treatment option for CRC.
Article
Oncology
Junya Arai, Ryota Niikura, Yoku Hayakawa, Nobumi Suzuki, Tetsuro Honda, Takuma Okamura, Kenkei Hasatani, Naohiro Yoshida, Tsutomu Nishida, Tetsuya Sumiyoshi, Shu Kiyotoki, Takashi Ikeya, Masahiro Arai, Narikazu Boku, Mitsuhiro Fujishiro
Summary: This multicenter retrospective cohort study found that probiotic use is not significantly associated with the duration of nivolumab treatment in all cancers, except for gastric cancer patients. Probiotic use was significantly linked to a longer duration of nivolumab treatment in gastric cancer patients. Thus, probiotics may improve the response to nivolumab and potentially prolong progression-free survival in patients with gastric cancer.
Article
Gastroenterology & Hepatology
Junki Tokura, Toshiyuki Yoshio, Shiro Hayashi, Masashi Yamamoto, Satoshi Asai, Takayuki Yakushijin, Kenji Ikezawa, Koji Nagaike, Tadayuki Takagi, Toshio Fujisawa, Takuya Yamada, Hidetaka Tsumura, Iruru Maetani, Yasuki Hori, Hideyuki Ihara, Kazuhiro Matsunaga, Toshio Kuwai, Yukiko Ito, Kenkei Hasatani, Yoriaki Komeda, Akira Kurita, Shinjiro Yamaguchi, Hirotsugu Maruyama, Takuji Iwashita, Mamoru Takenaka, Makoto Hosono, Tsutomu Nishida
Summary: This study aimed to establish diagnostic reference levels (DRLs) for gastrointestinal endoscopy procedures. By analyzing cases of gastrointestinal enteral metallic stent placement, the study established DRL values for different disease sites. The results showed that radiation exposure characteristics varied depending on the disease site during gastrointestinal enteral metallic stent placement.
Article
Gastroenterology & Hepatology
Shiro Hayashi, Mamoru Takenaka, Hirofumi Kogure, Takayuki Yakushijin, Yousuke Nakai, Kenji Ikezawa, Shinjiro Yamaguchi, Toshio Fujisawa, Yuzuru Tamaru, Iruru Maetani, Hirotsugu Maruyama, Satoshi Asai, Tadayuki Takagi, Koji Nagaike, Yasuki Hori, Tetsuya Sumiyoshi, Hidetaka Tsumura, Hisashi Doyama, Toshiyuki Yoshio, Kazuo Hara, Seiichiro Abe, Ichiro Oda, Motohiko Kato, Hiroko Nebiki, Tatsuya Mikami, Masanori Miyazaki, Kazuhiro Matsunaga, Makoto Hosono, Tsutomu Nishida
Summary: A follow-up survey conducted in Japan revealed some improvement in radiation protection equipment and education among endoscopy-fluoroscopy departments, although the progress was relatively limited compared to the previous survey in 2020.
Article
Oncology
Kana Hosokawa, Tsutomu Nishida, Daichi Hayashi, Miharu Kitazawa, Haruka Masuda, Katsuharu Tono, Yuhiko Katanosaka, Naohiro Sakamoto, Yoshifumi Fujii, Aya Sugimoto, Dai Nakamatsu, Kengo Matsumoto, Masashi Yamamoto, Koji Fukui
Summary: This study found no significant association between weight loss at diagnosis and prognosis in patients with advanced pancreatic cancer treated with best supportive care or chemotherapy.