Review
Endocrinology & Metabolism
Fritz Cayabyab, Lina R. Nih, Eiji Yoshihara
Summary: Diabetes is a complex global disease affecting over 400 million people, with pancreatic islet transplantation showing promise in treating type 1 diabetes but facing challenges such as immune rejection. Current research focuses on finding optimal transplantation sites and methods to improve islet graft survival, with the aim of translating these studies into successful clinical therapies.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Cell Biology
Diletta Overi, Guido Carpino, Marta Moretti, Antonio Franchitto, Lorenzo Nevi, Paolo Onori, Enrico De Smaele, Luca Federici, Daniele Santorelli, Marella Maroder, Lola M. Reid, Vincenzo Cardinale, Domenico Alvaro, Eugenio Gaudio
Summary: There is contrasting evidence on the contribution of stem/progenitor cell populations to pancreatic regeneration in diabetes, however, the PDG compartment may play a role in this process. T2DM patients show signs of pancreatic islet regeneration and involvement of the PDG niche, and PDX1 administration could support increased endocrine pancreatic regeneration in experimental diabetes. These findings contribute to defining the role and participation of stem/progenitor cell compartments within the pancreas.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Endocrinology & Metabolism
Kevin Verhoeff, Braulio A. Marfil-Garza, Zofia Czarnecka, Nerea Cuesta-Gomez, Ila Tewari Jasra, Nidheesh Dadheech, Peter A. Senior, A. M. James Shapiro
Summary: Historically, only patients with certain types of diabetes have been considered for islet transplantation. However, with the development of stem cell-derived transplantation and the reclassification of diabetes subtypes, it may soon be justifiable to consider a broader patient population for this procedure. This review evaluates the potential of islet transplantation for different diabetes subtypes and explores unique transplant features that need to be taken into account.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2023)
Review
Endocrinology & Metabolism
Meghan Tahbaz, Eiji Yoshihara
Summary: Insulin injection and human pancreatic islet transplantation are current main therapies for T1D or late-stage severe T2D, but limitations such as islet shortage and immunosuppression side effects call for the need to develop novel methods for generating protected functional human islets. Human pluripotent stem cells offer a potentially limitless cell source, and studying immune tolerance induction in hPSC-derived human pancreatic islets contributes to the goal of curing insulin-dependent diabetes.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Endocrinology & Metabolism
Pia Montanucci, Teresa Pescara, Alessia Greco, Giulia Leonardi, Luigi Marini, Giuseppe Basta, Riccardo Calafiore
Summary: The study found that hUCMS inhibited proliferation of allogeneic polymorphonuclear blood cells from patients with T1D, while promoting expansion of FoxP3(+)Tregs. Through co-microencapsulation, hUCMS and hIDC synergized anatomically and functionally, maintaining tracer insulin output by hIDC and utilizing the immunoregulatory properties of hUCMS to help decrease blood glucose levels.
DIABETES-METABOLISM RESEARCH AND REVIEWS
(2021)
Article
Cell & Tissue Engineering
Yasaman Aghazadeh, Frankie Poon, Farida Sarangi, Frances T. M. Wong, Safwat T. Khan, Xuetao Sun, Rupal Hatkar, Brian J. Cox, Sara S. Nunes, M. Cristina Nostro
Summary: This study enhanced islet transplantation by using microvessels from adipose tissue, resulting in improved cell survival and glucose response in both human islets and hESC-derived pancreatic cells, ameliorating preexisting diabetes in three mouse models of T1D.
Article
Cell & Tissue Engineering
Naoki Ishiuchi, Ayumu Nakashima, Satoshi Maeda, Yoshie Miura, Kisho Miyasako, Kensuke Sasaki, Toshio Uchiki, Ayano Sasaki, Shogo Nagamatsu, Naoki Nakao, Masataka Nagao, Takao Masaki
Summary: Fibrosis is a common histological feature in chronic organ injury, leading to organ failure. Adipose-derived mesenchymal stem cells (ASCs) have been studied as a therapeutic tool to prevent organ failure. This study compared the therapeutic effects of ASCs from superficial and deep layers and found that both types of ASCs are equally effective and safe for treating organ injury.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Multidisciplinary Sciences
Xiaojie Ma, Yunkun Lu, Ziyu Zhou, Qin Li, Xi Chen, Weiyun Wang, Yan Jin, Zhensheng Hu, Guo Chen, Qian Deng, Weina Shang, Hao Wang, Hongxing Fu, Xiangwei He, Xin-Hua Feng, Saiyong Zhu
Summary: Through chemical screening, the BET inhibitor I-BET151 is found to promote the expansion of pancreatic progenitors (PPs) and facilitate their differentiation into functional beta cells. Transplantation of these cells shows promising results in ameliorating diabetes. The study highlights the significance of epigenetic and transcriptional modulations in lineage-specific progenitor self-renewal.
Review
Multidisciplinary Sciences
Anneliese J. S. Flatt, Carla J. Greenbaum, James A. M. Shaw, Michael R. Rickels
Summary: Type 1 diabetes is characterized by loss and dysfunction of pancreatic islet beta cells, leading to insulin deficiency and hyperglycemia. Assessment of beta cell secretory capacity can detect the loss of beta cells during a presymptomatic phase of autoimmune attack. Islet transplantation can restore physiologic reserve capacity for insulin secretion.
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES
(2021)
Editorial Material
Cell Biology
Laura Crisa, Vincenzo Cirulli
Summary: In their recent paper, Chang and colleagues successfully achieved durable mixed hematopoietic chimerism that promotes the engraftment and long-term function of pancreatic islet allotransplants in fully immunocompetent mice without the need for immunosuppression.
Review
Pharmacology & Pharmacy
Feiyu Cai, Wenjiao Chen, Ruomei Zhao, Yi Liu
Summary: This paper provides an academic review of the therapeutic effects of adipose-derived stem cell-EXOs (ADSC-EXOs) in diabetic wound healing. As cell-free therapy, exosomes (EXOs) contain proteins and growth factors that can promote wound healing and mitigate potential risks associated with stem cell therapy.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Engineering, Biomedical
Sean M. Kinney, Krystal Ortaleza, Alexander E. Vlahos, Michael Sefton
Summary: This study demonstrates that islet transplantation can be achieved in the subcutaneous space using MAA-PEG hydrogel, without the need for vascularizing cells or growth factors. The survival of islet grafts through the inflammatory events of hydrogel degradation and islet revascularization highlights the benefits of the MAA biomaterial.
Review
Biochemistry & Molecular Biology
Serena Barachini, Letizia Biso, Shivakumar Kolachalam, Lacopo Petrini, Roberto Maggio, Marco Scarselli, Biancamaria Longoni
Summary: Pancreatic islet transplantation is a treatment for Type 1 diabetic patients, and mesenchymal stem cells (MSCs) have shown potential in controlling graft rejection. MSCs can interact with immune system cells through direct contact or their secretome. This review discusses the role of MSCs in controlling the immune system and promoting repair in pancreatic islet transplantation, as well as challenges such as oxidative stress and impaired vasculogenesis. Preconditioning MSCs with hypoxia exposure or using stem cells with angiogenic potential in organoids may help improve transplant outcomes.
Article
Endocrinology & Metabolism
Hiroo Sasaki, Hidetaka Suga, Kazuhito Takeuchi, Yuichi Nagata, Hideyuki Harada, Tatsuma Kondo, Eiji Ito, Sachi Maeda, Mayu Sakakibara, Mika Soen, Tsutomu Miwata, Tomoyoshi Asano, Hajime Ozaki, Shiori Taga, Atsushi Kuwahara, Tokushige Nakano, Hiroshi Arima, Ryuta Saito
Summary: We have successfully developed pituitary organoids (POs) derived from human embryonic stem cells (hESC). Transplantation of these POs into hypopituitary severe combined immunodeficient (SCID) mice was able to effectively restore ACTH secretion, thus providing a potential treatment for hypopituitarism.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Endocrinology & Metabolism
Yao Xie, Yongli Ji, Yunrui Lu, Yuankun Ma, Hui Ni, Jian Shen, Hong Ma, Chunna Jin, Yuwen Chen, Yan Lin, Meixiang Xiang
Summary: This study used single-cell analysis to compare the heterogeneity, functions, and cell fates of subcutaneous adipose-derived stem cells (SUB-ADSCs) and perivascular ADSCs (PV-ADSCs). Four subpopulations of PV-ADSCs were identified, with the Clec11a+ subpopulation potentially regulating the differentiation of PV-ADSCs towards a smooth muscle cell phenotype. The study revealed distinct characteristics between PV-ADSCs and SUB-ADSCs.
Article
Endocrinology & Metabolism
Subhadra C. Gunawardana, David W. Piston
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
(2015)
Article
Biochemistry & Molecular Biology
Nikki L. Farnsworth, Rachelle L. Walter, Alireza Hemmati, Matthew J. Westacott, Richard K. P. Benninger
JOURNAL OF BIOLOGICAL CHEMISTRY
(2016)
Article
Endocrinology & Metabolism
Richard K. P. Benninger, David J. Hodson
Article
Endocrinology & Metabolism
Linda M. Nguyen, Marina Pozzoli, Thomas H. Hraha, Richard K. P. Benninger
Article
Neurosciences
Nikki L. Farnsworth, Rachelle Walter, Robert A. Piscopio, Wolfgang E. Schleicher, Richard K. P. Benninger
JOURNAL OF PHYSIOLOGY-LONDON
(2019)
Article
Multidisciplinary Sciences
Shintaro Yamaguchi, Michael P. Franczyk, Maria Chondronikola, Nathan Qi, Subhadra C. Gunawardana, Kelly L. Stromsdorfer, Lane C. Porter, David F. Wozniak, Yo Sasaki, Nicholas Rensing, Michael Wong, David W. Piston, Samuel Klein, Jun Yoshino
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Article
Multidisciplinary Sciences
David G. Ramirez, Eric Abenojar, Christopher Hernandez, David S. Lorberbaum, Lucine A. Papazian, Samantha Passman, Vinh Pham, Agata A. Exner, Richard K. P. Benninger
NATURE COMMUNICATIONS
(2020)
Article
Cell Biology
Caryn Carson, Juan F. Macias-Velasco, Subhadra Gunawardana, Mario A. Miranda, Sakura Oyama, Celine L. St Pierre, Heather Schmidt, Jessica P. Wayhart, Heather A. Lawson
Article
Biochemical Research Methods
JaeAnn M. Dwulet, Jennifer K. Briggs, Richard K. P. Benninger
Summary: The study found that small populations of highly functional beta-cells may control islet-wide [Ca2+] dynamics, but through gap junction coupling, these small cell populations are unable to disproportionately affect the widespread [Ca2+] oscillations in the islet.
PLOS COMPUTATIONAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
David G. Ramirez, Mark Ciccaglione, Awaneesh K. Upadhyay, Vinh T. Pham, Mark A. Borden, Richard K. P. Benninger
Summary: Research shows that contrast ultrasound imaging with submicron nanodroplet phase-change agents can detect signs of insulitis before the onset of diabetes, and this technology is important for monitoring disease progression, guiding, and assessing preventative therapeutic interventions.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Endocrinology & Metabolism
Jose G. Miranda, Wolfgang E. Schleicher, Kristen L. Wells, David G. Ramirez, Samantha P. Landgrave, Richard K. P. Benninger
Summary: This study examines how patterns of electrical activity regulate NFAT activation, gene transcription, and islet function, and provides insights into how these factors are disrupted in diabetes.
MOLECULAR METABOLISM
(2022)
Article
Endocrinology & Metabolism
Jennifer K. Briggs, Anat Schonblum, Limor Landsman, Richard K. P. Benninger
Article
Acoustics
Vinh T. Pham, M. A. R. K. Ciccaglione, David G. Ramirez, Richard K. P. Benninger
Summary: The researchers previously found that measurements of pancreas perfusion dynamics can predict disease progression in type 1 diabetes (T1D). Now they tested whether these measurements can predict the success of therapeutic prevention of T1D.
ULTRASOUND IN MEDICINE AND BIOLOGY
(2022)
Article
Transplantation
Subhadra C. Gunawardana, David W. Piston
TRANSPLANTATION DIRECT
(2019)
Article
Endocrinology & Metabolism
Matthew J. Westacott, Nikki L. Farnsworth, Joshua R. St Clair, Greg Poffenberger, Audrey Heintz, Nurin W. Ludin, Nathaniel J. Hart, Alvin C. Powers, Richard K. P. Benninger