Article
Biochemistry & Molecular Biology
Eun-Jin Kim, Oh-Sang Kwon, Chang-Gi Hur, Marie Merci Nyiramana, Dong-Kun Lee, Seong-Geun Hong, Jaehee Han, Dawon Kang
Summary: This study identified the effect of GABAR agonists on K-2P channel expression and activity in neuroblastic B35 cells. Muscimol, a GABA(A)R agonist, was found to significantly increase TREK-2 expression and activity, potentially reducing cellular excitability in GABAergic neurons through activating the TREK-2 channel.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Electrochemistry
Yuanyuan Li, Hong Wei, Yan Chen, Jing Ma, Xinru Zhang-Peng, Wen Li, Fangdi Hu
Summary: In this study, a molecularly imprinted electrochemical sensor based on high-performance magnetically functionalized reduced graphene oxide (MRGO) was constructed for the sensitive detection of gamma-aminobutyric acid (GABA) in the serum of depressed mice. It provides a basis for the objective diagnosis of depression in clinic.
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
(2023)
Article
Plant Sciences
Zainab Waris, Zahra Noreen, Anis Ali Shah, Sheeraz Usman, Adnan Noor Shah, Muhammad Rizwan, Ryan Casini, Hosam O. O. Elansary
Summary: The research shows that cadmium can enter plants easily from the soil solution and cause harmful effects, even at low concentrations. The experiment conducted on lettuce (Lactuca sativa) demonstrates that the addition of γ-aminobutyric acid (GABA) can mitigate the toxicity of cadmium by promoting plant growth and enhancing antioxidant enzyme activity.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Article
Pharmacology & Pharmacy
Kenji Muroya, Kenta Ueda, Keiichi Wada, Masakazu Kotoda, Takashi Matsukawa
Summary: The study explores the potential of remimazolam in preventing shivering in hypothermic rabbits. Remimazolam at 1 mg/kg/h significantly lowered the core temperature change during shivering, which may act through the GABA pathway.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Zhen Wu, Pengyu Wang, Daodong Pan, Xiaoqun Zeng, Yuxing Guo, Guangsheng Zhao
Summary: The study demonstrated that GABA-enriched adzuki bean sprout fermented milk may alleviate and even prevent mild depression symptoms in mice by enhancing social interaction and pleasure derived from movement. The research also highlighted the involvement of the GABA(B)-cAMP-PKA-CREB signaling pathway and increased neurotransmitter levels in the hippocampus after treatment. This suggests a potential dietary therapy for chronic social stress.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Food Science & Technology
Weidan Guo, Zhengwen Li, Xiangjin Fu, Wenhua Zhou, Jiali Ren, Yue Wu
Summary: S. aureus contamination in sourdough has significant effects on metabolites, titratable acidity, and microbial diversity. It decreases the content of mannose, increases the content of sorbitol, reduces the number of lactic acid bacteria, decreases the acidity values, reduces the content of esters and acid flavor compounds, and increases the content of 2,4-decadienal. A PCA model using volatile metabolites data can be used to detect S. aureus contamination in sourdough.
Article
Biochemistry & Molecular Biology
Hyesook Lee, Seon Yeong Ji, Hyun Hwangbo, Min Yeong Kim, Da Hye Kim, Beom Su Park, Joung-Hyun Park, Bae-Jin Lee, Gi-Young Kim, You-Jin Jeon, Yung Hyun Choi
Summary: GABA-enriched salt has a protective effect against renal injury caused by high salt intake in cisplatin-induced nephrotoxicity mice, reducing hematological and biochemical toxicity, apoptosis, and inflammation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Environmental Sciences
Yunshi Liang, Yi Wu, Jianping Li, Ruibing Peng, Maowang Jiang, Xiamin Jiang, Siqing Chen, Jianyuan Lin
Summary: This study examined the effect of gamma-aminobutyric acid (GABA) as a supplement on the ammonia stress resistance of S. pharaonis. The results showed that the application of GABA significantly alleviated the adverse effects of ammonia poisoning, with the best effect observed at a dosage of 80-100 mg/kg.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Plant Sciences
Chujiang Zhou, Wanqi Dong, Shuwan Jin, Qingli Liu, Liyu Shi, Shifeng Cao, Saisai Li, Wei Chen, Zhenfeng Yang
Summary: The treatment of peach fruit with GABA can reduce chilling injury during cold storage by up-regulating the biosynthetic genes and candidate transcription factors related to ascorbic acid and glutathione.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Clinical Neurology
James Fasham, Antje K. Huebner, Lutz Liebmann, Reham Khalaf-Nazzal, Reza Maroofian, Nderim Kryeziu, Saskia B. Wortmann, Joseph S. Leslie, Nishanka Ubeyratna, Grazia M. S. Mancini, Marjon van Slegtenhorst, Martina Wilke, Tobias B. Haack, Hanan E. Shamseldin, Joseph G. Gleeson, Mohamed Almuhaizea, Imad Dweikat, Bassam Abu-Libdeh, Muhannad Daana, Maha S. Zaki, Matthew N. Wakeling, Lucy McGavin, Peter D. Turnpenny, Fowzan S. Alkuraya, Henry Houlden, Peter Schlattmann, Kai Kaila, Andrew H. Crosby, Emma L. Baple, Christian A. Huebner
Summary: A novel neurodevelopmental disorder associated with biallelic pathogenic variants in SLC4A10 has been identified, which exhibits learning difficulties, seizures, and characteristic brain imaging features. Dysfunction of GABAergic transmission may contribute to the disorder.
Article
Behavioral Sciences
Sum Yi Ma, Kin Ming Kwan
Summary: The study found that early prenatal exposure to VPA in mice led to tail deformation and decreased cerebellar weight and size, with reduced expression of GAD 65 and GAD 67 in the cerebellum of adult mice showing autistic behavior. Additionally, protein expressions of GAT-1 and GABAT were reduced in VPA mice, suggesting alterations in GABA production, recycling, and metabolism that could disrupt the excitatory-inhibitory balance in the autistic cerebellum.
BEHAVIOURAL BRAIN RESEARCH
(2022)
Article
Chemistry, Applied
Zahra Sadat Asgarian, Rouhollah Karimi, Mehdi Ghabooli, Masoomeh Maleki
Summary: The study found that using 40 mM concentration of GABA can effectively reduce weight loss, rachis browning, and decay incident in grapes during storage, while increasing antioxidant enzyme activity and decreasing membrane electrolyte leakage, leading to higher soluble sugars, organic acids, and phenolic compounds.
Article
Food Science & Technology
Wee Yin Koh, Xiao Xian Lim, Eva Sheue Wen Teoh, Rovina Kobun, Babak Rasti
Summary: This study aimed to develop GABA-enriched dark chocolate by partially replacing sugar syrup with pure GABA powder. The addition of 0.15% GABA significantly increased the GABA content and ACE inhibitory effect of chocolate. The quality of GABA-enriched chocolate was similar to regular chocolate in terms of nutritional, physical, shelf-life, and sensorial properties.
Article
Medicine, Research & Experimental
Nathaniel Miller, Michael Noller, Alex Yang, Edward D. McCoul, Anthony M. Tolisano, Charles A. Riley
Summary: This study comprehensively summarized and synthesized the existing evidence for lesser known uses of gabapentin and pregabalin in otolaryngology. The most promising potential uses identified were for improving swallowing, trismus, and narcotic overuse after radiation therapy. Additionally, GABA analogues showed potential in improving nonorganic voice disorders, but the benefit for globus pharyngeus related to laryngeal sensory neuropathy remains inconclusive.
Article
Biotechnology & Applied Microbiology
Lei Sun, Yingguo Bai, Xiu Zhang, Cheng Zhou, Jie Zhang, Xiaoyun Su, Huiying Luo, Bin Yao, Yuan Wang, Tao Tu
Summary: Three recombinant GAD genes from Bacillus strains were identified and successfully expressed heterologously in Escherichia coli. Using the engineered E. coli strain as a whole-cell biocatalyst, the biosynthesis of GABA from L-Glu was achieved, showing potential for industrial production of GABA.
MICROBIAL CELL FACTORIES
(2021)
Article
Physiology
Stefania Fozzato, Nicolo Baranzini, Elena Bossi, Raffaella Cinquetti, Annalisa Grimaldi, Paola Campomenosi, Michele Francesco Surace
Summary: The study aimed to investigate the presence of nervous fibers and expression of TRP channels in samples harvested during spinal surgeries from patients with chronic low back pain (CLBP). It found that TRPV4 and TRPM8 channels were consistently upregulated in pathological tissues, suggesting they may play a role in CLBP and should be further investigated as potential therapeutic targets.
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY
(2021)
Letter
Respiratory System
E. Zampogna, G. B. Migliori, R. Centis, F. Cherubino, C. Facchetti, D. Feci, G. Palmiotto, P. Pignatti, L. Saderi, G. Sotgiu, A. Spanevello, M. Zappa, D. Visca
Article
Infectious Diseases
E. Zampogna, N. Ambrosino, R. Centis, F. Cherubino, G. B. Migliori, P. Pignatti, G. Lo Bello, L. Saderi, G. Sotgiu, M. Zappa, A. Spanevello, D. Visca
Summary: The 6-min walking test (6MWT) is responsive to physiological changes and pulmonary rehabilitation in patients with asthma, with the minimal clinically important difference (MCID) determined to be 26m in this study.
INTERNATIONAL JOURNAL OF TUBERCULOSIS AND LUNG DISEASE
(2021)
Article
Critical Care Medicine
Elisabetta Zampogna, Patrizia Pignatti, Nicolino Ambrosino, Francesca Cherubino, Anna Maria Fadda, Martina Zappa, Antonio Spanevello, Dina Visca
Summary: The 5-repetition sit-to-stand test (5STS) has been shown to be effective in evaluating the outcomes of pulmonary rehabilitation in patients with asthma, indicating its potential usefulness in assessing the effects of treatment for this condition.
Article
Biochemical Research Methods
Raffaella Cinquetti, Francesca Guia Imperiali, Salvatore Bozzaro, Daniele Zanella, Francesca Vacca, Cristina Roseti, Barbara Peracino, Michela Castagna, Elena Bossi
Summary: A novel method using fluorescence quenching was developed to measure substrate uptake, allowing for simultaneous testing of multiple oocytes in different experimental conditions and collection of statistically significant data for each batch, ultimately saving time and animals.
Article
Biochemistry & Molecular Biology
Michela Castagna, Raffaella Cinquetti, Tiziano Verri, Francesca Vacca, Matteo Giovanola, Amilcare Barca, Tiziana Romanazzi, Cristina Roseti, Alessandra Galli, Elena Bossi
Summary: The SLC6 family includes 20 human transporters classified into neurotransmitter transporters and nutrient amino acid transporters. Insects' orthologs of SLC6 family transporters have served as important tools for investigating mammalian counterparts.
NEUROCHEMICAL RESEARCH
(2022)
Review
Cell Biology
Algerta Marku, Alessandra Galli, Paola Marciani, Nevia Dule, Carla Perego, Michela Castagna
Summary: Iron is crucial for insulin synthesis and ATP production in pancreatic beta-cells. Proper regulation of iron content is essential to prevent oxidative damage and cell dysfunction. Understanding and controlling iron metabolism in beta-cells could lead to new treatments for diseases such as diabetes.
Article
Clinical Neurology
Ludovica Iovino, Veronica Giusti, Francesca Pischedda, Elena Giusto, Nicoletta Plotegher, Antonella Marte, Ilaria Battisti, Angela Di Iacovo, Algerta Marku, Giovanni Piccoli, Rina Bandopadhyay, Carla Perego, Tiziana Bonifacino, Giambattista Bonanno, Cristina Roseti, Elena Bossi, Giorgio Arrigoni, Luigi Bubacco, Elisa Greggio, Sabine Hilfiker, Laura Civiero
Summary: This study demonstrates that the pathogenic variant G2019S of leucine-rich repeat kinase 2 (LRRK2) impairs the function of Excitatory Amino Acid Transporter 2 (EAAT2), resulting in glutamate overload in the brain, which may contribute to neurodegeneration in Parkinson's disease (PD).
ACTA NEUROPATHOLOGICA
(2022)
Article
Neurosciences
Francesca Vacca, Ana S. Gomes, Koji Murashita, Raffella Cinquetti, Cristina Roseti, Amilcare Barca, Ivar Ronnestad, Tiziano Verri, Elena Bossi
Summary: Two slc15a2-type genes were identified in the Atlantic salmon genome, encoding PepT2-type peptide transporters. While slc15a2a transcripts are widely distributed in fish tissues, abundant in the brain and gills, slc15a2b transcripts are mainly expressed in the kidney and distal gastrointestinal tract. Both PepT2a and PepT2b function as high-affinity transporters.
JOURNAL OF PHYSIOLOGY-LONDON
(2022)
Review
Biochemistry & Molecular Biology
Manan Bhatt, Angela Di Iacovo, Tiziana Romanazzi, Cristina Roseti, Raffaella Cinquetti, Elena Bossi
Summary: The heterologous expression of proteins in Xenopus laevis oocytes remains essential in many research fields. New approaches and revised protocols are applied to study membrane transporters, and both new and classic methods for investigating the activity and expression of Solute Carriers (SLC) are reviewed.
Review
Pharmacology & Pharmacy
Manan Bhatt, Angela Di Iacovo, Tiziana Romanazzi, Cristina Roseti, Elena Bossi
Summary: The role and function of betaine in the brain have been little studied and reviewed, despite its well-documented effects in the liver and kidney. This review analyzes the beneficial effects of betaine in neurodegeneration, excitatory and inhibitory imbalance, and oxidative stress in the central nervous system. However, there are still many unknown aspects regarding its transport and the actions beyond the known protein, betaine-GABA transporter 1. This article highlights the lack of detailed molecular and cellular information and the importance of studying the interaction of betaine with CNS components for its therapeutic use.
BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY
(2023)
Article
Neurosciences
Tiziana Romanazzi, Daniele Zanella, Manan Bhatt, Angela Di Iacovo, Aurelio Galli, Elena Bossi
Summary: Bile acids (BAs) synthesized from cholesterol in the liver play a key role in emulsifying fats for absorption. Recent evidence suggests that BAs can modulate the activity of neuronal receptors and transporters, including the dopamine transporter (DAT), involved in gut-brain signaling. This study investigated the effects of BAs on three transporters and found that obeticholic acid (OCA), a semi-synthetic BA, generated an inward current in DAT, GABA transporter 1 (GAT1), and glycine transporter 1 (GlyT1b), proportional to the current generated by their respective substrates. The findings support a molecular model suggesting the ability of BAs to occlude the transporter, avoiding small depolarizations and enhancing neurotransmitter action.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2023)
Review
Physiology
Manan Bhatt, Laure Gauthier-Manuel, Erika Lazzarin, Rocco Zerlotti, Christine Ziegler, Andre Bazzone, Thomas Stockner, Elena Bossi
Summary: γ-Aminobutyric acid (GABA) acts as the primary inhibitory neurotransmitter in the CNS, regulated by GABA transporters (GATs). Although GAT1 has been extensively studied, the roles of other GABA transporters, especially BGT-1, remain unclear. This review aims to compare GAT1 and BGT-1, leveraging the knowledge of GAT1 to shed light on the unanswered questions regarding BGT-1.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Physiology
Francesca Vacca, Ana S. Gomes, Marco De Gennaro, Ivar Ronnestad, Elena Bossi, Tiziano Verri
Summary: The transport capability of charged dipeptides in teleosts is related to the position of the charged amino acid. In Atlantic salmon, the transport currents of PepT1a and PepT1b are similar, while in zebrafish, PepT1a is highly dependent on the position of Asp and PepT1b has low transport currents.
FRONTIERS IN PHYSIOLOGY
(2023)
Meeting Abstract
Biochemistry & Molecular Biology
Ludovica Iovino, Veronica Giusti, Francesca Pischedda, Elena Giusto, Nicoletta Plotegher, Antonella Marte, Ilaria Battisti, Angela Di Iacovo, Algerta Marku, Giovanni Piccoli, Rina Bandopadhyay, Carla Perego, Tiziana Bonifacino, Giambattista Bonanno, Cristina Roseti, Elena Bossi, Giorgio Arrigono, Luigi Bubacco, Elisa Greggio, Sabine Hilfiker, Laura Civiero
JOURNAL OF NEUROCHEMISTRY
(2022)