Article
Multidisciplinary Sciences
Alyssa M. Klein, Lynn Biderman, David Tong, Bita Alaghebandan, Sakina A. Plumber, Helen S. Mueller, Anne van Vlimmeren, Chen Katz, Carol Prives
Summary: This study suggests that MDM2 and MDMX may play a p53-independent role in maintaining cell-cycle progression by affecting the activity of E2F family members and p73, making them a potential target of interest in cancers lacking wild-type p53.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Cell Biology
Hongchang Li, Chaonan Li, Wenjing Zhai, Xin Zhang, Lei Li, Bo Wu, Biyue Yu, Pengfei Zhang, Jie Li, Chun-Ping Cui, Lingqiang Zhang
Summary: Autophagy is essential for neonatal energy homeostasis and survival. The deubiquitylase ubiquitin-specific peptidase 10 (USP10) destabilizes TP53 to regulate neonatal autophagy and viability.
Article
Oncology
Quanzhu Chen, Qian Peng, Jing Cai, Ying Liu, Xingxing Lu, Wei Xiong, Zhaoyang Zeng, Guiyuan Li, Xiaoling Li, Xiayu Li, Bo Xiang, Mei Yi, Pan Chen
Summary: Nasopharyngeal carcinoma (NPC) is a malignant tumor with high metastatic and invasive nature. Super enhancers (SEs) control the expression of cell identity genes and oncogenes during tumorigenesis. Hyaluronan synthase 3 (HAS3) is overexpressed in NPC and promotes malignant features via the EGFR/AKT/ERK signaling pathway.
Article
Biochemistry & Molecular Biology
Li Yuan, Shibing Li, Qiuyan Chen, Tianliang Xia, Donghua Luo, Liangji Li, Sailan Liu, Shanshan Guo, Liting Liu, Chaochao Du, Guodong Jia, Xiaoyun Li, Zijian Lu, Zhenchong Yang, Huanliang Liu, Haiqiang Mai, Linquan Tang
Summary: This study found that Epstein-Barr virus (EBV) infection reduces the sensitivity of nasopharyngeal carcinoma (NPC) cells to ferroptosis by activating the p62-Keap1-NRF2 signaling pathway and upregulating SLC7A11 and GPX4 expression. Inhibition of GPX4 enhances the chemosensitivity of EBV-infected NPC cells. High GPX4 expression is associated with poor clinical outcomes in NPC and other cancer types.
CELL DEATH AND DIFFERENTIATION
(2022)
Article
Biochemistry & Molecular Biology
Yang Liu, Yuewen Luo, Shumei Yan, Yi-Fan Lian, Shiyu Wu, Miao Xu, Lin Feng, Xu Zhang, Rong Li, Xiantao Zhang, Qi-Sheng Feng, Yi-Xin Zeng, Hui Zhang
Summary: In this study, the role of KLHDC3 in non-small cell lung cancer (NSCLC) was investigated. Higher expression of KLHDC3 was associated with worse overall survival in NSCLC patients. KLHDC3 deficiency affected the proliferation of NSCLC cell lines and its interaction with CUL2 E3 ligase and tumor suppressor p14ARF. Gefitinib-resistant NSCLC cell lines displayed higher KLHDC3 protein levels. Furthermore, KLHDC3 shortage increased the sensitivity of lung cancer cells to EGFR-targeted drugs.
Article
Oncology
Xu Lu, Xing Chen, Xinke Wang, Jing Qing, Ji Li, Yunyun Pan
Summary: This study identifies independent prognostic factors for nasopharyngeal carcinoma and provides potential candidate biomarkers for diagnosis, prognosis, or precise treatment of this cancer through gene expression analysis and network construction.
FRONTIERS IN ONCOLOGY
(2022)
Article
Pathology
Cecilia Taverna, Abbas Agaimy, Alessandro Franchi
Summary: The MDM2 gene plays a significant role in the development of nasopharyngeal carcinoma. MDM2 could be a valuable diagnostic marker to support the diagnosis of nasopharyngeal EBV positive NKSCC.
PATHOLOGY RESEARCH AND PRACTICE
(2022)
Article
Cell Biology
Yajie Zhao, Chao Li, Yan Zhang, Zhanzhan Li
Summary: CircTMTC1 is highly expressed and miR-495 is downregulated in nasopharyngeal carcinoma (NPC), which are associated with poor prognosis. CircTMTC1 promotes NPC progression through targeting miR-495 and activating the MET-eIF4G1 translational regulation axis.
CELL DEATH & DISEASE
(2022)
Article
Cell Biology
Chen-Yan Wu, Lei Li, Shi-Lu Chen, Xia Yang, Chris Zhiyi Zhang, Yun Cao
Summary: Clear cell renal cell carcinoma (ccRCC) is a common malignancy with rapid growth and high metastasis and lacks effective therapeutic targets. This study demonstrates that the Zic2/Runx2/NOLC1 signaling axis plays a crucial role in promoting ccRCC progression, providing potential therapeutic targets and prognostic indicators. Patients with high Zic2/Runx2 and low NOLC1 expression have the worst outcomes according to Kaplan-Meier survival analyses.
CELL DEATH & DISEASE
(2021)
Article
Oncology
Rui Shi, Zirong Liu
Summary: RPL15 plays crucial roles in the progression and metastasis of hepatocellular carcinoma (HCC), serving as a promising candidate for targeted therapies.
CANCER CELL INTERNATIONAL
(2022)
Article
Immunology
Pan Chen, Dan Wang, Tengfei Xiao, Wangning Gu, Hongmin Yang, Minghua Yang, Hui Wang
Summary: This study explored the effect of acyl-CoA synthetase long-chain family member 4 (ACSL4) on the pathogenesis of nasopharyngeal carcinoma (NPC) through the crosstalk between ferroptosis and tumor-associated macrophages (TAMs). It was found that ACSL4 inhibited the development of NPC by activating ferroptosis and altering the phenotype of TAMs. These findings provide a new potential direction for NPC therapy.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Oncology
Haiwen Chen, Xiaobing Duan, Xiaohong Deng, Yingping Huang, Xiang Zhou, Shanshan Zhang, Xiao Zhang, Pingjuan Liu, Chaopin Yang, Guojun Liu, Qinqin Ren, Yan Xiong, Bo Zhu, Jiexia Zhang, Tong Xiang
Summary: Nasopharyngeal carcinoma is an EBV-associated epithelial malignancy characterized by infiltration of lymphocytes, including NK cells. EBV-positive NPC cells often develop mechanisms to evade NK cell immune surveillance. Understanding the mechanisms involved in EBV-induced NK cell dysfunction may lead to the development of novel immunotherapies for NPC.
CANCER IMMUNOLOGY RESEARCH
(2023)
Article
Cell Biology
Chun Cheng, Jun Yang, Si-Wei Li, Guofu Huang, Chenxi Li, Wei-Ping Min, Yi Sang
Summary: Histone deacetylases (HDACs) are involved in tumor progression, with HDAC4 playing a crucial role in promoting cell proliferation, migration, and invasion in nasopharyngeal carcinoma (NPC). High expression of HDAC4 in NPC tissues is associated with poor overall survival and progression-free survival. Inhibition of HDAC4 may present a potential therapeutic strategy for NPC patients.
CELL DEATH & DISEASE
(2021)
Article
Cell Biology
Md Wasim Khan, Alexander R. Terry, Medha Priyadarshini, Vladimir Ilievski, Zeenat Farooq, Grace Guzman, Jose Cordoba-Chacon, Issam Ben-Sahra, Barton Wicksteed, Brian T. Layden
Summary: HKDC1, highly expressed in liver cancer cells, plays a key role in the development and progression of liver cancer by promoting metabolic reprogramming and disrupting mitochondrial function. Targeting HKDC1, specifically its interaction with mitochondria, may provide a selective approach to target liver cancer cells.
CELL DEATH & DISEASE
(2022)
Article
Medicine, General & Internal
Shang-Xin Liu, Chong Wang, Ruo-Bin Lin, Wei-Yue Ding, Gaurab Roy, Hong-Bo Wang, Ting Yang, Qian Liu, Yi-Ling Luo, Shui-Lin Jin, Mu-Sheng Zeng, Bo Zhao, Qian Zhong
Summary: This study revealed the critical role of super-enhancers associated with SOX2 in the tumorigenesis of nasopharyngeal carcinoma (NPC). High expression of SOX2 was correlated with poor prognosis. Inhibition of SOX2 suppressed tumor growth, highlighting the potential of SOX2 as a therapeutic target for NPC patients.
Article
Environmental Sciences
Chia-Lung Shih, Han-Chung Wu
Summary: Increased concentrations of heavy metals in the southwestern coast of Taiwan were observed due to human activities and economic growth. However, there are limited studies on the seasonal variations of major chemical parameters and their associations in this region. This study investigated the seasonal variation and associations of major chemical parameters in the coastal waters and estuaries. The results showed that the Donggang River was slightly polluted based on the level of dissolved oxygen (DO), while the Pachang River and Gaoping River were slightly polluted based on suspended solids.
ENVIRONMENTAL FORENSICS
(2022)
Article
Biochemistry & Molecular Biology
Ruey-Herng Lee, Yu-Jen Wang, Ting-Yen Lai, Tsui-Ling Hsu, Po-Kai Chuang, Han-Chung Wu, Chi-Huey Wong
Summary: The combination of anti-SSEA4 and anti-Globo H antibodies can suppress tumor growth in breast cancer cells. SSEA4 is identified as a major target in breast cancer due to higher expression levels compared to Globo H. An antibody designed to maximize ADCC activity can enhance the killing efficiency of NK cells.
ACS CHEMICAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jia-Hsin Huang, You-Rou Liao, Tzu-Chieh Lin, Cheng-Hung Tsai, Wei-Yun Lai, Yang-Kai Chou, Jun-Yi Leu, Huai-Kuang Tsai, Cheng-Fu Kao
Summary: iTARGEX, a new association framework, can identify novel regulators involved in biological traits without prior functional annotations. Experimental validation of the novel candidates provides solid evidence for their roles in the respective traits, converting genome-wide observations into causal gene function predictions. Further application of iTARGEX is expected to facilitate the discovery of new regulators and provide observations for novel mechanistic hypotheses regarding different biological traits and phenotypes.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Microbiology
Cheng-Pu Sun, Jia-Tsrong Jan, I-Hsuan Wang, Hsiu-Hua Ma, Hui-Ying Ko, Ping-Yi Wu, Tzu-Jiun Kuo, Hsin-Ni Liao, Yu-Hua Lan, Zong-Lin Sie, Yen-Hui Chen, Yi-An Ko, Chun-Che Liao, Liang-Yu Chen, I-Jung Lee, Szu- Tsung, Yun-Ju Lai, Ming-Tsai Chiang, Jian-Jong Liang, Wen-Chun Liu, Jing-Rong Wang, Joyce Pei-Yi Yuan, Yin-Shiou Lin, Yi-Ching Tsai, Shie-Liang Hsieh, Chia-Wei Li, Han-Chung Wu, Tai-Ming Ko, Yi-Ling Lin, Mi-Hua Tao
Summary: This study introduces a versatile approach that enables rapid generation of mouse models for emerging infectious diseases within a month, aiding in the study of disease mechanisms and drug development. The created mouse model for COVID-19 accurately mimicked severe cases in patients and was successfully used in drug development.
Article
Oncology
Yi-Ping Wang, I-Ju Liu, Kai-Chi Chen, Han-Chung Wu
Summary: In squamous cell carcinoma of head and neck (SCCHN), high expression of HER3 and its ligand NRG1 is associated with increased regional lymphatic metastasis. HER3 is positively regulated by NOTCH1 through AKT pathway and USP8 deubiquitinating enzyme to inhibit protein degradation. Exclusion of patients with NOTCH1-inactivated SCCHN may benefit future clinical trials targeting HER3.
Article
Gastroenterology & Hepatology
Hsi-Chien Huang, Yun-Chieh Sung, Chung-Pin Li, Dehui Wan, Po-Han Chao, Yu-Ting Tseng, Bo-Wen Liao, Hui-Teng Cheng, Fu-Fei Hsu, Chieh-Cheng Huang, Yi-Ting Chen, Yu-Hui Liao, Hsin Tzu Hsieh, Yu-Chuan Shih, I-Ju Liu, Han-Chung Wu, Tsai-Te Lu, Jane Wang, Yunching Chen
Summary: This study aimed to develop a nanotherapy that delivers TRAIL and NO to PDAC to overcome stromal barriers and improve therapeutic efficacy. Results showed that delivering NO to the PDAC tumour stroma reprogrammed stellate cells, reduced desmoplasia, and enhanced TRAIL penetration, leading to significant antitumour effects.
Article
Biochemistry & Molecular Biology
Ruei-Min Lu, Shih-Han Ko, Wan-Yu Chen, Yu-Ling Chang, Hsiu-Ting Lin, Han-Chung Wu
Summary: Mitigation strategies for the COVID-19 pandemic have been hindered by the emergence of SARS-CoV-2 variants. A panel of 41 monoclonal antibodies against SARS-CoV-2 NP was generated, with 9 high-binding antibodies used in a new rapid diagnostic test showing high sensitivity and specificity for detecting viral variants. The developed LFIA strips have the capability to detect 10 NP mutants, including the alpha, beta, gamma, and delta variants.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Chih-Wei Lin, Yu-Jen Wang, Ting-Yen Lai, Tsui-Ling Hsu, Shin-Ying Han, Han-Chung Wu, Chia-Ning Shen, Van Dang, Ming-Wei Chen, Lan-Bo Chen, Chi-Huey Wong
Summary: The study reveals that SSEA-4 is overexpressed in pancreatic cancer cells and associated with survival rates; targeting SSEA-4 with antibodies or CAR-T cells shows promise in inhibiting cancer growth and could be a potential immunotherapy target for pancreatic disease treatment.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Microbiology
Shih-Chieh Su, Tzu-Jing Yang, Pei-Yu Yu, Kang-Hao Liang, Wan-Yu Chen, Chun-Wei Yang, Hsiu-Ting Lin, Mei-Jung Wang, Ruei-Min Lu, Hsien-Cheng Tso, Meng-Jhe Chung, Tzung-Yang Hsieh, Yu-Ling Chang, Shin-Chang Lin, Fang-Yu Hsu, Feng-Yi Ke, Yi-Hsuan Wu, Yu-Chyi Hwang, I-Ju Liu, Jian-Jong Liang, Chun-Che Liao, Hui-Ying Ko, Cheng-Pu Sun, Ping-Yi Wu, Jia-Tsrong Jan, Yuan-Chih Chang, Yi-Ling Lin, Mi-Hua Tao, Shang-Te Danny Hsu, Han-Chung Wu
Summary: The study developed a series of chimeric antibodies targeting the RBD of the SARS-CoV-2 spike protein, with some exhibiting exceptionally potent neutralizing activities. Cryo-electron microscopy analyses of two highly potent antibodies complexed with the SARS-CoV-2 spike protein suggested they may be particularly useful when combined in a cocktail therapy.
Article
Biology
Sarah G. Swygert, Dejun Lin, Stephanie Portillo-Ledesma, Po-Yen Lin, Dakota R. Hunt, Cheng-Fu Kao, Tamar Schlick, William S. Noble, Toshio Tsukiyama
Summary: The study found that an increase in the range of local inter-nucleosomal contacts in quiescent yeast drives genome-wide compaction of chromatin fibers, leading to global repression of transcription. Unlike actively dividing cells, inter-nucleosomal interactions in quiescent cells require a specific basic patch in the histone H4 tail.
Article
Biochemical Research Methods
Kai-Li Chang, Jia-Hong Chen, Tzu-Chieh Lin, Jun-Yi Leu, Cheng-Fu Kao, Jin Yung Wong, Huai-Kuang Tsai
Summary: This study uses the deep learning model "DeepCircle" to analyze the characteristics and predictability of short extrachromosomal circular DNAs (eccDNAs) in the genome. It finds that although eccDNAs do not show apparent specificity in genomic locations, they exhibit similarity in sequence features across different datasets, indicating a certain level of predictability for eccDNAs.
BRIEFINGS IN BIOINFORMATICS
(2023)
Review
Cell Biology
Yu-Chyi Hwang, Ruei-Min Lu, Shih-Chieh Su, Pao-Yin Chiang, Shih-Han Ko, Feng-Yi Ke, Kang-Hao Liang, Tzung-Yang Hsieh, Han-Chung Wu
Summary: The COVID-19 pandemic has created a public health crisis, leading to the urgent development of therapeutic treatments and virus detection methods. Monoclonal antibodies (mAbs) have emerged as powerful tools for treating and detecting diseases due to their high specificity and reliability. Researchers are urgently developing antibody-based kits for SARS-CoV-2 detection and antibody drugs for COVID-19 treatment. The spike protein of SARS-CoV-2, which is crucial for viral infection, has been extensively studied and its receptor-binding domain (RBD) has become a major target for therapeutic antibody development. Given the high mutation rate of SARS-CoV-2, especially under the pressure of prophylactic vaccines and neutralizing antibodies, the use of antibody cocktails is expected to be an important strategy for effective COVID-19 treatment. Additionally, antibodies against cytokine storms, which can be triggered by SARS-CoV-2 infection and drive severe disease progression, are also being developed as treatments for COVID-19. In addition to their use as drugs, antibodies are currently being used in SARS-CoV-2 detection tests, including antigen and immunoglobulin tests, which are crucial surveillance tools for preventing the spread of COVID-19.
JOURNAL OF BIOMEDICAL SCIENCE
(2022)
Article
Cell Biology
Kang-Hao Liang, Pao-Yin Chiang, Shih-Han Ko, Yu-Chi Chou, Ruei-Min Lu, Hsiu-Ting Lin, Wan-Yu Chen, Yi-Ling Lin, Mi-Hua Tao, Jia-Tsrong Jan, Han-Chung Wu
Summary: The study showed that six antibodies had varying binding and neutralizing abilities against SARS-CoV-2 variants, with antibody cocktails demonstrating potent neutralizing activities and potential therapeutic and prophylactic effects against multiple variants.
JOURNAL OF BIOMEDICAL SCIENCE
(2021)
Review
Genetics & Heredity
Chia-Ling Hsu, Yi-Chen Lo, Cheng-Fu Kao
Summary: During the aging process, H3K4 methylation (H3K4me) may be linked to metabolic pathways and play a conservative role in determining organism longevity across different species, but its influence is context-specific and requires further investigation.
Article
Cell Biology
Jing-You Guo, I-Ju Liu, Hsiu-Ting Lin, Mei-Jung Wang, Yu-Ling Chang, Shin-Chang Lin, Mei-Ying Liao, Wei-Chia Hsu, Yi-Ling Lin, James C. Liao, Han-Chung Wu
Summary: By using phage display technology and enzyme-linked immunosorbent assays, 36 enriched peptides were identified in the sera of two COVID-19 patients, with 4 motifs having consensus residues corresponding to two potential B-cell epitopes on SARS-CoV-2 viral proteins. These findings may aid in investigating COVID-19 pathogenesis and advancing the development of epitope-based serological diagnostics and vaccines.
JOURNAL OF BIOMEDICAL SCIENCE
(2021)