Review
Biochemistry & Molecular Biology
Rachel L. Washburn, Jannette M. Dufour
Summary: Sertoli cells play a crucial role in creating an immunoregulatory environment for spermatogenesis and protecting germ cells from immune destruction. This review focuses on the understudied complement system and its regulation by Sertoli cells. Studies in transplantation models have shown that Sertoli cells can survive activated complement, express complement inhibitors, and modulate immune cell infiltration. Understanding the mechanism of Sertoli cell-mediated complement regulation is important for male reproduction, autoimmunity, and transplantation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biology
David Fleck, Lina Kenzler, Nadine Mundt, Martin Strauch, Naofumi Uesaka, Robert Moosmann, Felicitas Bruentgens, Annika Missel, Artur Mayerhofer, Dorit Merhof, Jennifer Spehr, Marc Spehr
Summary: The study reveals that coordinated contractions of smooth muscle-like testicular peritubular cells provide the propulsive force for luminal sperm transport towards the rete testis. Spontaneous tubular contractions and peristaltic transport are shown to be causally related to peritubular Ca2+ waves. Purinergic signaling pathways are identified as physiological triggers of tubular contractions both in vitro and in vivo.
Review
Pathology
Xu You, Qian Chen, Ding Yuan, Changcheng Zhang, Haixia Zhao
Summary: By detailing the characteristics and applications of more than 20 Sertoli cell markers, this review provides novel Sertoli cell markers for the more accurate diagnosis and mechanistic evaluation of male reproductive disorders.
EXPERT REVIEW OF MOLECULAR DIAGNOSTICS
(2021)
Article
Medicine, General & Internal
O. Sena Aydos, Yunus Yukselten, Tulin Ozkan, Sinan Ozkavukcu, Meltem Tuten Erdogan, Asuman Sunguroglu, Kaan Aydos
Summary: This study found that adding FSH/testosterone to the culture medium can promote the differentiation and maturation of germ cells, and co-culturing with healthy SCs does not provide additional benefits. Additionally, the freezing-thawing process does not affect the viability and proliferation of SCs.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Agriculture, Dairy & Animal Science
Jahaira Cortez, Barbara Leiva, Cristian G. Torres, Victor H. Parraguez, Monica De los Reyes, Albert Carrasco, Oscar A. Peralta
Summary: This study presents the first report on the generation and characterization of bovine testicular organoids (TOs), which could be useful tools for evaluating the impact of exogenous factors on reproductive physiology in domestic and wild cattle.
Article
Reproductive Biology
Feng Chen, Yingjie Wu, Lu Ke, Xinyi Lin, Fengchao Wang, Yinghe Qin
Summary: This study revealed that specific deletion of Arhgef15 in Sertoli cells of mice leads to sperm abnormality, disruption of BTB integrity, and potentially affects testicular immune homeostasis.
BIOLOGY OF REPRODUCTION
(2022)
Article
Developmental Biology
Annika Missel, Lena Walenta, Katja Eubler, Nadine Mundt, Hanna Heikela, Ulrich Pickl, Matthias Trottmann, Bastian Popper, Matti Poutanen, Leena Strauss, Frank-Michael Koehn, Lars Kunz, Marc Spehr, Artur Mayerhofer
Summary: The presence of ATP metabolites and adenosine receptors in human testicular peritubular cells suggests their involvement in cytokine production and potential impact on fertility and male infertility.
MOLECULAR HUMAN REPRODUCTION
(2021)
Article
Endocrinology & Metabolism
Wiwat Rodprasert, Jaakko J. Koskenniemi, Helena E. Virtanen, Sergey Sadov, Antti Perheentupa, Helena Ollila, Jakob Albrethsen, Anna Maria Andersson, Anders Juul, Niels E. Skakkebaek, Katharina M. Main, Jorma Toppari
Summary: There are differences in pubertal hormone levels between boys with a history of cryptorchidism who experienced spontaneous testicular descent or underwent orchiopexy and boys without a history of cryptorchidism.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2022)
Article
Developmental Biology
Annika Missel, Lena Walenta, Katja Eubler, Nadine Mundt, Hanna Heikela, Ulrich Pickl, Matthias Trottmann, Bastian Popper, Matti Poutanen, Leena Strauss, Frank-Michael Koehn, Lars Kunz, Marc Spehr, Artur Mayerhofer
Summary: Extracellular ATP has been found to play a role in cytokine production in human testicular cells, with adenosine possibly involved as a metabolite. Pharmacological inhibition of ENTPD1 reduced pro-inflammatory cytokines, suggesting a link to ATP metabolites like adenosine. Targeting ATP metabolites or adenosine receptors could potentially reduce inflammatory events in the testis, offering novel therapeutic avenues for male infertility treatment.
MOLECULAR HUMAN REPRODUCTION
(2021)
Article
Cell Biology
Gerald R. Cunha, Mei Cao, Sena Aksel, Amber Derpinghaus, Laurence S. Baskin
Summary: It has been assumed that mouse organogenesis can serve as a model for human organogenesis, but there are significant anatomical, morphogenetic, and molecular differences between mice and humans. This study compares the development of testicles in mice and humans, focusing on differences in the formation of different cell types. It provides a detailed comparison of early testicular development in mice and humans.
Article
Immunology
Anneke Wilharm, Helena C. Brigas, Inga Sandrock, Miguel Ribeiro, Tiago Amado, Annika Reinhardt, Abdi Demera, Lisa Hoenicke, Till Strowig, Tania Carvalho, Immo Prinz, Julie C. Ribot
Summary: This study identified a previously underappreciated fetal-derived subset of γδ17 cells that infiltrate the testis at steady state, expand upon puberty, and play a crucial role in local tissue immune surveillance.
MUCOSAL IMMUNOLOGY
(2021)
Article
Cell Biology
Annika Liebich, Nina Schmid, Christina Koupourtidou, Carola Herrmann, Kim-Gwendolyn Dietrich, Harald Welter, Jovica Ninkovic, Artur Mayerhofer
Summary: Peritubular cells of the human testis play important roles in sperm transport and spermatogonial stem cell niche. In this study, single-cell RNA sequencing was used to study cultured human testicular peritubular cells (HTPCs) isolated from donors with normal spermatogenesis. The expression profile of HTPCs revealed cell plasticity and identified four cellular states. The findings provide insights into the functions of human testicular peritubular cells and serve as a resource for further understanding male (in)fertility.
Article
Environmental Sciences
Weiqi Sun, Fang Tian, Hongjie Pan, Xiuli Chang, Minjie Xia, Jingying Hu, Yuzhu Wang, Runsheng Li, Weihua Li, Mingjun Yang, Zhijun Zhou
Summary: This study systematically explored the male reproductive toxicity of FLC and found that it caused testicular dysfunction and damage in mice, leading to abnormal spermatogenesis and decreased sperm quality. The potential mechanisms include interference with spermatogonial proliferation and meiosis, as well as apoptosis and mitochondrial damage in testicular Sertoli cells.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Biochemistry & Molecular Biology
Xiaomei Xie, Longjie Sun, Yixi Duan, Zheng Lv, Xiaohong Yao, Chaofan Wang, Xuexue Chen, Shuang Tian, Lu Yan, Yujing Shao, Haoshu Luo, Jiali Liu
Summary: SRSF2 plays a crucial role in supporting testis development and normal spermatogenesis. Its deficiency impairs fetal Sertoli cell proliferation, induces abnormal apoptosis and severe DNA damage, resulting in testicular dysplasia, seminiferous tubule atrophy, and infertility.
Article
Biochemistry & Molecular Biology
Amitabha Sengupta, Manveet Nanda, Shanza Baseer Tariq, Tania Kiesel, Kayla Perlmutter, Margarita Vigodner
Summary: Sumoylation plays a crucial role in regulating survival and signaling pathways in Sertoli cells, with KAP1 potentially acting as a major regulator of sumoylation in these cells.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Cardiac & Cardiovascular Systems
Jan-Per Wenzel, Ramona Bei der Kellen, Christina Magnussen, Stefan Blankenberg, Benedikt Schrage, Renate Schnabel, Julius Nikorowitsch
Summary: The study evaluated the prevalence and correlates of left ventricular diastolic dysfunction (DD) in a middle-aged sample of the general population. The results showed that DD without HFpEF (ALVDD) was associated with hypertension and HbA1c, while DD with HFpEF (DDwHFpEF) was associated with factors like female sex, atrial fibrillation, and coronary artery disease.
CLINICAL RESEARCH IN CARDIOLOGY
(2022)
Article
Dentistry, Oral Surgery & Medicine
Julia Struppek, Carolin Walther, Kubra Bunte, Birgit-Christiane Zyriax, Jan-Per Wenzel, Juliana Senftinger, Julius Nikorowitsch, Guido Heydecke, Udo Seedorf, Thomas Beikler, Katrin Borof, Carola Mayer, Ghazal Aarabi
Summary: This study examined the association between coffee consumption and periodontitis in a population in Hamburg. The results showed a significant link between heavy coffee consumption and periodontitis, suggesting that high coffee intake may be a potential risk factor for the disease.
CLINICAL ORAL INVESTIGATIONS
(2022)
Article
Agriculture, Dairy & Animal Science
Hassan A. Hussein, Gerhard Schuler, Theresa Conze, Axel Wehrend
Summary: This study compared the consistency of two commercial on-site methods and a radioimmunoassay in measuring blood progesterone concentration. The results showed that the two commercial on-site methods exhibited high consistency with the radioimmunoassay reference method and can be beneficially used in a clinical setting.
Review
Endocrinology & Metabolism
Hiba Hasan, Sudhanshu Bhushan, Monika Fijak, Andreas Meinhardt
Summary: Infection and inflammation are relevant factors that can lead to male reproductive disorders, causing damage to the testis and affecting spermatogenesis and steroidogenesis. Increased levels of oxidative stress and pro-inflammatory cytokines negatively impact germ cells. The activation of inflammatory pathways is a common feature in male testicular disorders beyond established infectious/inflammatory cues.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Wei Peng, Artem Kepsch, Till O. Kracht, Hiba Hasan, Rukmali Wijayarathna, Eva Wahle, Christiane Pleuger, Sudhanshu Bhushan, Stefan Guenther, A. Christine Kauerhof, Ana Planinic, Daniela Fietz, Hans-Christian Schuppe, Malgorzata Wygrecka, Kate L. Loveland, Davor Jezek, Andreas Meinhardt, Mark P. Hedger, Monika Fijak
Summary: Experimental autoimmune-orchitis (EAO), a rodent model of chronic testicular inflammation and fibrosis, replicates pathogenic changes seen in some cases of human spermatogenic disturbances. CCR2 and activin A promote fibrosis during testicular inflammation by regulating macrophage function.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Cardiac & Cardiovascular Systems
Nina Fluschnik, Enver Tahir, Jennifer Erley, Kai Muellerleile, Andreas Metzner, Jan-Per Wenzel, Helena Guerreiro, Gerhard Adam, Stefan Blankenberg, Paulus Kirchhof, Tobias Toennis, Julius Nikorowitsch
Summary: Pending verification in independent series, clinically indicated MRI scans at 3T field strength should not be withheld from patients with cardiac pacemakers or defibrillators, as our data suggest that it is safe and does not result in adverse events in most cases.
Article
Nutrition & Dietetics
Carola Mayer, Felix L. L. Naegele, Marvin Petersen, Maximilian Schell, Ghazal Aarabi, Thomas Beikler, Katrin Borof, Benedikt M. M. Frey, Julius Nikorowitsch, Juliana Senftinger, Carolin Walther, Jan-Per Wenzel, Birgit-Christiane Zyriax, Bastian Cheng, Goetz Thomalla
Summary: The study found that consuming 3-4 cups of coffee per day is associated with fewer neurodegenerative disorders and improved microstructural brain integrity. However, the relationship between coffee consumption and vascular brain damage and neurodegeneration remains unclear. Further research is needed to determine the long-term effects of coffee on brain microstructure.
Article
Biology
Christiane Pleuger, Dingding Ai, Minea L. Hoppe, Laura T. Winter, Daniel Bohnert, Dominik Karl, Stefan Guenther, Slava Epelman, Crystal Kantores, Monika Fijak, Sarina Ravens, Ralf Middendorff, Johannes U. Mayer, Kate L. Loveland, Mark Hedger, Sudhanshu Bhushan, Andreas Meinhardt, Wei Yan
Summary: The epididymis exhibits region-specific immune responses, with inflammation being more pronounced in the distal regions compared to the proximal regions. Through studies using a mouse model and analysis of human cells, it was found that resident immune cells in the epididymis show significant heterogeneity in distribution, potentially providing different immunological environments to address immunological challenges.
Article
Cardiac & Cardiovascular Systems
Kishore Surendra, Sylvia Nuernberg, Jan P. Bremer, Marius S. Knorr, Frank Ueckert, Jan Per Wenzel, Ramona Bei Der Kellen, Dirk Westermann, Renate B. Schnabel, Raphael Twerenbold, Christina Magnussen, Paulus Kirchhof, Stefan Blankenberg, Johannes Neumann, Benedikt Schrage
Summary: The study aims to develop a practical screening tool for heart failure at the general population level. By using a convolutional neural network (CNN) built on digital electrocardiograms (ECGs), the researchers achieved comparable predictive accuracy for heart failure as the logistic regression model and the random forest classifier. The study suggests that this screening tool could be integrated into the clinical routine of general practitioners, alleviating the underdiagnosis of heart failure.
Letter
Cardiac & Cardiovascular Systems
Marc D. Lemoine, Celine Mencke, Moritz Nies, Julius Obergassel, Katharina Scherschel, Hartwig Wieboldt, Ruben Schleberger, Ilaria My, Laura Rottner, Julia Moser, Shinwan Kany, Jan-Per Wenzel, Fabian Moser, Leon Dinshaw, Paula Muenkler, Bruno Reissmann, Feifan Ouyang, Christian Meyer, Stefan Blankenberg, Tanja Zeller, Larissa Fabritz, Andreas Rillig, Andreas Metzner, Paulus Kirchhof
CIRCULATION-ARRHYTHMIA AND ELECTROPHYSIOLOGY
(2023)
Article
Multidisciplinary Sciences
Juliana Senftinger, Julius Nikorowitsch, Katrin Borof, Francisco Ojeda, Ghazal Aarabi, Thomas Beikler, Carola Mayer, Christian-Alexander Behrendt, Carolin Walther, Birgit-Christiane Zyriax, Raphael Twerenbold, Stefan Blankenberg, Jan-Per Wenzel
Summary: This study aimed to evaluate the associations between coffee consumption and cardiac biomarkers, echocardiographic, electrocardiographic parameters, and major cardiovascular diseases. The results showed that high coffee consumption was associated with higher LDL-cholesterol levels and lower systolic and diastolic blood pressure, but not with major cardiovascular diseases.
SCIENTIFIC REPORTS
(2023)
Article
Cardiac & Cardiovascular Systems
Laura Rottner, Julius Obergassel, Katrin Borof, Ilaria My, Fabian Moser, Marc Lemoine, Jan-Per Wenzel, Paulus Kirchhof, Feifan Ouyang, Bruno Reissmann, Andreas Metzner, Andreas Rillig
Summary: This study compared the efficiency, fluoroscopy exposure, and dye volume of KODEX-EPD guided cryoballoon pulmonary vein isolation (CB-PVI) using a novel saline injection-based workflow with conventional cryoablation. It was found that KODEX-EPD guided CB-PVI without the need for dye injection resulted in reduced fluoroscopy exposure compared to conventional cryoablation, with no differences in acute efficacy or procedure duration.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2023)
Letter
Cardiac & Cardiovascular Systems
Jan-Per Wenzel, Marc D. Lemoine, Laura Rottner, Ilaria My, Fabian Moser, Julius Obergassel, Moritz Nies, Jan Riess, Djemail Ismaili, Julius Nikorowitsch, Feifan Ouyang, Paulus Kirchhof, Andreas Rillig, Andreas Metzner, Bruno Reissmann
CIRCULATION-ARRHYTHMIA AND ELECTROPHYSIOLOGY
(2023)
Article
Cardiac & Cardiovascular Systems
Ilaria My, Marc D. Lemoine, Mahi Butt, Celine Mencke, Fabian W. Loeck, Julius Obergassel, Laura Rottner, Jan-Per Wenzel, Ruben Schleberger, Julia Moser, Fabian Moser, Paulus Kirchhof, Bruno Reissmann, Feifan Ouyang, Andreas Rillig, Andreas Metzner
Summary: This study compared the acute lesion extent after pulmonary vein isolation (PVI) induced by pulsed-field ablation (PFA) and multielectrode radiofrequency balloon (RFB). The results showed that PFA resulted in larger ablation areas and higher release of cardiac injury biomarkers compared to RFB.
JOURNAL OF CARDIOVASCULAR ELECTROPHYSIOLOGY
(2023)
Meeting Abstract
Cardiac & Cardiovascular Systems
S. Camen, L. Nagel, R. Bei Der Kellen, E. Barow, J. N. Schneider, S. Blankenberg, P. Kirchhof, J. P. Wenzel, G. Thomalla, R. B. Schnabel
EUROPEAN HEART JOURNAL
(2022)