Article
Biochemical Research Methods
Ji Min Kim, Jung Soo Park, Tae Hwi Yoon, Junghun Park, Ki Soo Park
Summary: The PCR-based lateral flow assay developed in this study enables rapid and multiplex detection of hygiene indicator bacteria with high sensitivity and specificity, making it suitable for food hygiene monitoring in the food industry.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Mustafa Ture, Muhammed Baran Kilic, Ilhan Altinok
Summary: The study investigated the impact of heavy metal pollution on the levels of Cd, Co, Cu, Ni, and Zn in the muscle of whiting and mullet, as well as the presence of heavy metal resistance genes in bacteria. Results showed variations in metal levels in the fish muscle across different seasons, and differences in the distribution of heavy metal resistance genes in bacteria.
BIOLOGICAL TRACE ELEMENT RESEARCH
(2021)
Article
Environmental Sciences
Maria Jose Saavedra, Conceicao Fernandes, Amilcar Teixeira, Xana Alvarez, Simone Varandas
Summary: Freshwater mussels are highly endangered due to the overuse of antibiotics, which has led to the emergence of antibiotic-resistant bacteria. These resistant bacteria increase the risk associated with the use of surface water and the spread of resistance genes. A study on two endangered freshwater mussel species revealed that coliform bacteria were present in both the water samples and the mussels, with the lowest concentration found at site T1. One of the mussel species, P. littoralis, faced the highest concentration of bacterial toxins, primarily from treated wastewater effluents and population centers. The bacteria isolates from this species showed resistance to multiple antibiotics, highlighting the importance of investigating management measures and water quality for freshwater bivalves.
ENVIRONMENTAL POLLUTION
(2022)
Article
Microbiology
Michael Schalli, Sarah Maria Inwinkl, Sabine Platzer, Rita Baumert, Franz F. Reinthaler, Petra Ofner-Kopeinig, Doris Haas
Summary: This study developed an alternative method for the challenging growth of microorganisms on CCA. The alternative method using CV-supplemented CCA showed similar values in sensitivity, specificity, and selectivity compared to the reference method. Repeatability and reproducibility of the alternative method were acceptable, and it showed advantages in the exclusion study.
Article
Chemistry, Multidisciplinary
Hanyu Qian, Eric Mclamore, Nikolay Bliznyuk
Summary: Reuse of alternative water sources for irrigation is a sustainable approach to reduce water gaps and increase food production, but it poses a risk of bacterial contamination. This study developed a statistical machine-learning framework to accurately predict the concentration of pathogenic organisms, such as E. coli, in untreated surface water using an impedimetric electrochemical aptasensor. The framework improved prediction accuracy and can be used for rapid decision support in hydroponic irrigation.
Article
Biotechnology & Applied Microbiology
Laurel L. Dunn, Loretta M. Friedrich, Laura K. Strawn, Michelle D. Danyluk
Summary: During the 2013 cantaloupe season, inspections were conducted in response to the 2011 Listeria monocytogenes outbreak. Testing in Florida cantaloupe packinghouses revealed low prevalence of L. monocytogenes compared to other food environments. Traditional indicators such as TPC, coliforms, E. coli and Listeria spp. were not reliable for detecting L. monocytogenes contamination in these packinghouses.
Article
Chemistry, Multidisciplinary
Veronika Lehotova, Veronika Antalkova, Alzbeta Medved'ova, L'ubomir Valik
Summary: Microbiological analysis during stretched cheese production revealed significant increases in microbial populations during critical steps, followed by reductions in viable counts during steaming/stretching process. Yeasts and molds showed the best indicatory function during storage of vacuum-packaged cheeses at 6 degrees C.
APPLIED SCIENCES-BASEL
(2021)
Article
Multidisciplinary Sciences
Delia Tomoiaga, Jaclyn Bubnell, Liam Herndon, Paul Feinstein
Summary: Griffith proposed the concept of DNA transfer between bacteria in 1928. Researchers have demonstrated that E. coli can accept multiple plasmids simultaneously, resulting in widespread aclonality during colony development. Tracking clonally related bacteria allows for mosaic analysis of gene function. Additionally, it was discovered that clonally related bacterium rods self-organize fractally and can remain linked during colony growth, presenting a potential target for controlling microbiota growth.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Analytical
Haoran Li, Yubo Wang, Yaohua Li, Wei Wang
Summary: The new method successfully quantifies bacterial viability at a single-cell level using optical tweezers, holding potential in antibiotic susceptibility testing, drug screening, and rapid diagnostics.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Haoran Li, Yubo Wang, Yaohua Li, Wei Wang
Summary: This study presents a new label-free bacterial viability assay based on optical tweezers technology, which quantifies bacterial viability by monitoring bacterial nanomotion in the optical traps. The assay is characterized by short time consumption, simple sample preparations, and no cytotoxicity, making it a reliable method for assessing bacterial viability at the single-cell level.
ANALYTICAL CHEMISTRY
(2022)
Article
Biotechnology & Applied Microbiology
Etinosa Osemwowa, Iyekhoetin Matthew Omoruyi, Paula Kurittu, Annamari Heikinheimo, Maria Fredriksson-Ahomaa
Summary: The study reveals a significant difference in bacterial contamination levels on raw beef surfaces between Nigeria and Finland, with Nigerian samples showing higher bacterial counts than Finnish samples, and the majority of Nigerian samples being unacceptable according to EU standards. Furthermore, beef samples from Nigeria have higher contamination rates of Salmonella, STEC, and CREC compared to samples from Finland.
Review
Infectious Diseases
Fabio P. Sellera, Luciano C. B. A. Da Silva, Nilton Lincopan
Summary: The COVID-19 pandemic has deepened the relationships between humans and companion animals, leading to an increase in the use of antibiotics and biocides worldwide. Data from global surveillance studies show a linear trend in increasing carbapenem resistance among Gram-negative pathogens in companion animals, with humans potentially acquiring carbapenemase-producing bacteria through contact with infected animals or contaminated environments. The rapid spread of WHO critical priority carbapenemase-producing pathogens in companion animals presents a One Health challenge for the post-pandemic world.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Article
Environmental Sciences
Geethani Eragoda Arachchilage Amarawansha, Francis Zvomuya, Annemieke Farenhorst
Summary: The study found that homes on First Nations reserves in Manitoba, Canada, using cisterns for water supply had tap water more contaminated with coliform bacteria compared to homes receiving piped water from treatment plants. Particularly, belowground concrete and fiberglass cisterns had higher levels of Escherichia coli and total coliform contamination.
ENVIRONMENTAL MONITORING AND ASSESSMENT
(2021)
Article
Multidisciplinary Sciences
Muhammad Tanveer Munir, Nattar Maneewan, Julien Pichon, Mohammed Gharbia, Ismael Oumarou-Mahamane, Jessica Baude, Chantal Thorin, Didier Lepelletier, Patrice Le Pape, Matthieu Eveillard, Mark Irle, Helene Pailhories, Florence Aviat, Christophe Belloncle, Michel Federighi, Laurence Dubreil
Summary: The study used mCherry Staphylococcus aureus as a model bacterium and employed a confocal spectral laser microscope (CSLM) to characterize wood surfaces. It was found that more bacteria were present near the surface of the LT face wood, and this methodology shows promise as a tool for hygienic safety evaluation.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
James Y. Tan, Tatyana E. Saleski, Xiaoxia Nina Lin
Summary: Co-cultivation in microfluidic droplets is a versatile tool for studying microbial communities, and the droplet size is a critical parameter that affects various aspects of community dynamics.
