Journal
AMERICAN JOURNAL OF BOTANY
Volume 99, Issue 7, Pages E268-E273Publisher
BOTANICAL SOC AMER INC
DOI: 10.3732/ajb.1100569
Keywords
BAC end; Citrus clementina; genetic mapping; indels; phylogenetic analysis; Rutaceae
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Funding
- French ANR genomic 'Citrusseq,'
- Spanish Ministerio de Ciencia e Innovacion [PSE-060000-2009-8, IPT-010000-2010-43, AGL2008-00596-MCI]
- Generalidad Valenciana [2008/121]
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Premise of the study: Indel markers were developed from BAC-end sequences of Citrus clementina cv. Nules. Transferability and polymorphism were tested in the Citrus genus to estimate the potential of indel markers mined from a single genotype for use in genetic studies. Methods and Results: Using polyacrylamide gel electrophoresis and DNA silver staining, 89 indel markers were tested for their transferability and polymorphism. Thirty-eight markers were selected. Heterozygosity in C. clementina cv. Nules was confirmed for 33 of these indel pairs. A preliminary diversity study using a capillary electrophoresis fragment analyzer was conducted with 21 indels using 45 accessions representing Citrus genus diversity. Intraspecific and interspecific polymorphisms were observed. Conclusions: These results indicate the utility of indel markers developed from sequence data of a single genotype of interspecific origin. In Citrus, these markers will be useful for genetic mapping, germplasm characterization, and phylogenetic assignment of DNA fragments.
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