Journal
AMERICAN JOURNAL OF BOTANY
Volume 98, Issue 8, Pages E224-E225Publisher
BOTANICAL SOC AMER INC
DOI: 10.3732/ajb.1100052
Keywords
Incarvillea sinensis; microsatellite; polymorphism; self-fertilization; subcloning
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Funding
- Youth Foundation of Institute of Botany, CAS [1101166]
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Premise of the study: Microsatellite markers were developed for Incarvillea sinensis var. sinensis (Bignoniaceae), an annual herb endemic to Inner Mongolia, to study the degree to which delayed self-fertilization is favored. Methods and Results: Eight polymorphic primer sets were isolated and characterized in two Inner Mongolia populations of I. sinensis var. sinensis with a relatively simple and fast subcloning method. Numbers of alleles per locus ranged from 2 to 7, with observed and expected heterozygosities ranging from 0 to 0.261 and from 0 to 0.778, respectively. Conclusions: These markers will be useful for future studies of self-fertilization adaptability in I. sinensis var. sinensis.
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