Article
Biochemistry & Molecular Biology
Yan Liu, Fan Zhang, Ling Jiang, J. Jefferson P. Perry, Zhihe Zhao, Jiayu Liao
Summary: This study introduces a novel method to determine kinetics parameters of product inhibition using quantitative FRET(qFRET) assay, providing a convenient way to determine all kinetics parameters and a new approach to combine different measurements with mutually compatible results and errors for the first time.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Chemistry, Multidisciplinary
Qiong Liu, Jingjun Lin, Hui Cheng, Liling Wei, Fuxian Wang
Summary: A potassium and rubidium co-modified carbon nitride (CN-KRb) was reported for the simultaneous co-photocatalytic CO2 reduction and ethanol oxidation to produce the same product, CH3CHO. The CN-KRb showed record photocatalytic activity and high selectivity, with the involvement of rubidium and potassium. The study confirmed the participation of H+ from ethanol oxidation in the CO2 reduction process, achieving near ideal overall atomic economy. This work provides a new strategy for effective and sustainable conversion of CO2 into the same product through photoexcited electron and hole utilization.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Multidisciplinary
Daisuke Kawaguchi, Hitoshi Ogihara, Hideki Kurokawa
Summary: In this study, 1,1-diethoxyethane (DEE) was selectively synthesized by the electrolysis of ethanol using a proton-exchange membrane (PEM) reactor, achieving the direct acetalization of ethanol. High faradaic efficiency of DEE was obtained through sequential electrochemical and nonelectrochemical reactions without the need for chemical reagents. This electrochemical approach provides a sustainable process for upgrading bioethanol to chemicals by utilizing renewable electricity.
Article
Biology
Chong Sha, Qiang Wang, Hongcheng Wang, Yilan Duan, Chongmao Xu, Lian Wu, Kesen Ma, Weilan Shao, Yu Jiang
Summary: This study identified that Fe-AAdh and Fe-Adh2 are the main enzymes responsible for ethanol production in Tne, while Zn-Adh plays a major role in ethanol consumption. These findings could potentially facilitate the development of a novel hyperthermophilic strain for cellulosic ethanol production.
Article
Biochemistry & Molecular Biology
Ramazan Kalin, Zeynep Koksal, Songul Bayrak, Serpil Gerni, Isil Nihan Ozyurek, Hande Usanmaz, Muhammet Karaman, Ali Atasever, Hasan Ozdemir, Ilhami Gulcin
Summary: This study tested the inhibition effects of several antibiotics on lactoperoxidase (LPO) and found that cefazolin exhibited the strongest inhibitory efficacy. The study also identified the key role of the 5-methyl-1,3,4-thiadiazol-2-yl moiety in the inhibition mechanism of cefazolin.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Article
Biochemical Research Methods
Hezi Hayun, Valeria Arkadash, Amiram Sananes, Eyal Arbely, David Stepensky, Niv Papo
Summary: This study improved the pharmacokinetic profile of TIMP2 by PEGylation, achieving site-specific monoPEGylation. The result showed that PEGylated TIMP2 had an extended half-life without impairing its inhibitory activity against MMP-14.
BIOCONJUGATE CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Andrew E. Huckleby, Jhawn G. Saul, Hyunshun Shin, Staci Desmarais, Apparao Bokka, Junha Jeon, Sung-Kun Kim
Summary: This study demonstrated the inhibitory effect of two compounds with two hydroxamate functional groups on MBL from Bacillus anthracis. The inhibition was found to be competitive and the compounds showed potential as inhibitors to overcome bacterial resistance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Green & Sustainable Science & Technology
Yimin Deng, Shuo Li, Lise Appels, Huili Zhang, Nick Sweygers, Jan Baeyens, Raf Dewil
Summary: Catalytic steam reforming of ethanol (CSRE) is a promising method for hydrogen production. Ethanol can be derived from low-cost carbohydrates or biomass through fermentation. This study explores the use of novel non-noble catalysts, impregnated in alpha-Al2O3, for CSRE. The catalytic experiments demonstrate high hydrogen yields and negligible catalyst deactivation. Pilot-scale investigations have been initiated.
RENEWABLE & SUSTAINABLE ENERGY REVIEWS
(2023)
Article
Biochemistry & Molecular Biology
Lian Xu, Li-Chao Wang, Bing-Mei Su, Xin-Qi Xu, Juan Lin
Summary: By combining the enhancement of the acetaldehyde elimination system with enzyme self-assembly strategy, the biosynthesis of (2S, 3R)-MPS was accelerated, resulting in a significantly increased yield of 248.1 mM. This promising and green route offers a high potential for industrial production of this valuable compound.
BIOORGANIC CHEMISTRY
(2021)
Article
Chemistry, Medicinal
Daniel Kovner, Inn H. Yuk, Amy Shen, Hong Li, Tobias Graf, Sanjay Gupta, Wenqiang Liu, Anthony Tomlinson
Summary: Enzymatic hydrolysis of polysorbate in drug products poses a major challenge for the biopharmaceutical industry. This study aimed to identify and characterize host cell proteins (HCPs) responsible for polysorbate hydrolysis and their substrate specificity. Twenty recombinant HCPs were assessed for their hydrolytic activity against a fluorogenic esterase substrate and polysorbate in a representative monoclonal antibody formulation. The results provide valuable insights for the development of LC-MS/MS approaches to identify polysorbate-degrading enzymes and improve purification processes to remove such HCPs.
JOURNAL OF PHARMACEUTICAL SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Suresh Pal, Bryce Plapp
Summary: X-ray crystallography has revealed that the threonine residue (Thr-45) in the yeast alcohol dehydrogenase (ADH1) plays an important role in catalysis and its substitution results in a significant decrease in catalytic efficiency. Conformational changes of the enzyme and ligand exchanges on the catalytic zinc are severely impacted by the removal of the hydroxyethyl group of Thr-45.
CHEMICO-BIOLOGICAL INTERACTIONS
(2021)
Article
Multidisciplinary Sciences
Sani Muhammad Uzairu, Yahaya Tijani, Madu Adamu Gadaka, Babagana Modu, Miriam Watafua, Hadiza Ali Ahmad, Umar Abdullahi Zakariya, Aminu Ibrahim, Aliyu Daja, Hassan Zanna, Abdullahi Balarabe Sallau
Summary: The study reveals the potential of methylrosmarinate as a good inhibitor of BChE and its possible therapeutic application in Alzheimer's disease. Using molecular docking simulations and experimental validation, the research elucidates the mechanism and structural features of methylrosmarinate-BChE interactions.
Article
Chemistry, Physical
Franziska Stegemann, Martin Grininger
Summary: This study investigates the mechanisms of FASs and PKSs in synthesizing important products and the substrate selectivity and enzymatic properties of different ATs. Findings reveal that ATs in PKS systems occurring in bacteria are slower than ATs in mammalian FASs, reflecting the respective purposes and metabolic contexts within the organism.
Article
Microbiology
Miha Bahun, Natasa Poklar Ulrih
Summary: The propeptide of hyperthermophilic archaeal subtilase pernisine strongly inhibits pernisine with high selectivity, highlighting its significant role in regulating subtilase maturation.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Miha Bahun, Natasa Poklar Ulrih, Jing Han
Summary: The propeptide of pernisine strongly inhibits pernisine and shows high selectivity towards pernisine compared to homologous subtilisin Carlsberg and proteinase K.
MICROBIOLOGY SPECTRUM
(2023)