Article
Biochemistry & Molecular Biology
Ashley G. Boice, Karla E. Lopez, Raj K. Pandita, Melissa J. Parsons, Chloe Charendoff, Vijay Charaka, Alexandre F. Carisey, Tej K. Pandita, Lisa Bouchier-Hayes
Summary: The study demonstrates that caspase-2 is activated in dividing cells in G1 phase of the cell cycle and plays a crucial role in maintaining genomic stability by protecting cells from replication stress and promoting replication fork protection. These functions are independent of its pro-apoptotic role and involve regulating cell cycle and DNA repair events to prevent the accumulation of DNA damage.
Article
Biochemistry & Molecular Biology
Ignacio Rodriguez-Pastrana, Eleni Birli, Amanda S. Coutts
Summary: The tumor suppressor p53 plays a crucial role in the cellular response to DNA damage, including cell cycle arrest, apoptosis, and DNA repair. JMY, an actin nucleator and DNA damage-responsive protein, undergoes nuclear accumulation during DNA damage. Through transcriptomics, we identified that JMY is required for the regulation of key p53 target genes involved in DNA repair. Lack or knockout of JMY leads to increased DNA damage and JMY's actin nucleation function is necessary for promoting the clearance of DNA lesions. This study suggests a role for actin in JMY's nuclear activity during the DNA damage response.
CELL DEATH AND DIFFERENTIATION
(2023)
Review
Chemistry, Inorganic & Nuclear
Nahyun Kwon, Dayeh Kim, K. M. K. Swamy, Juyoung Yoon
Summary: Reactive oxygen species (ROS) and reactive nitrogen species (RNS) play crucial roles in human life, but overproduction can lead to cell damage and diseases. Researchers have developed fluorescent and luminescent probes based on metal-coordinated systems to detect and image these active species, providing valuable tools for studying their roles in signaling and pathology.
COORDINATION CHEMISTRY REVIEWS
(2021)
Review
Biochemistry & Molecular Biology
Xiaochen Xie, Ye Zhang, Zhuo Wang, Shanshan Wang, Xiaoyou Jiang, Hongyan Cui, Tingting Zhou, Zheng He, Hao Feng, Qiqiang Guo, Xiaoyu Song, Liu Cao
Summary: ROS, initially considered pathological, are now recognized as signaling intermediates that promote cellular adaptation to stress through autophagy regulation. ATM protein, activated by ROS, is involved in autophagy regulation, maintaining genome stability and triggering cytoplasmic autophagy as a ROS sensor.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Review
Immunology
Irene Gonzalez-Jimenez, David S. Perlin, Erika Shor
Summary: The production of reactive oxidant species (ROS) in fungal cells upon exposure to antifungal drugs is still not well understood. The chemical nature, molecular origins, and functional consequences of these ROS need to be further investigated. Studies have shown that ROS contribute to the fungicidal activities of antifungal drugs and may be involved in programmed cell death (PCD). However, the primary role of pro-death ROS in the antifungal mechanism of action is still unclear. ROS induction may also have an adaptive role in promoting antifungal drug tolerance and the evolution of drug resistance. Filling these knowledge gaps will provide new insights into fungal biology and potential strategies to enhance antifungal drug activity or prevent drug resistance.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Oncology
Hugh A. Nicholson, Lynne Sawers, Rosemary G. Clarke, Kevin J. Hiom, Michelle J. Ferguson, Gillian Smith
Summary: This study found that individuality in FGF1 expression influences chemotherapy response and survival in ovarian cancer patients. The inhibition of FGF signaling can reverse drug resistance, while FGF1 over-expression induces resistance. Combination therapy using inhibitors can enhance sensitivity to platinum drugs.
BRITISH JOURNAL OF CANCER
(2022)
Review
Chemistry, Multidisciplinary
Chenyang Zhang, Xin Wang, Jiangfeng Du, Zhanjun Gu, Yuliang Zhao
Summary: ROS play a vital role in physiological and pathological processes, and research on regulating ROS for disease treatments has gained wide attention. Advances in nanotechnology have led to the development of numerous nanomaterials with the ability to regulate ROS, aiming to find new and effective ROS-related nanotherapeutic modalities. Despite significant progress in ROS-based nanomedicines, there is a need to address fundamental principles for designing ROS-associated nanomedicines to minimize gaps in biomedical applications. This review summarizes current progress in ROS-associated nanomedicines in disease treatments and presents key principles for their design. Future perspectives in the development of ROS-associated nanomedicines are also discussed.
Article
Oncology
Matteo Lulli, Laura Del Coco, Tommaso Mello, Caecilia Sukowati, Stefania Madiai, Laura Gragnani, Paolo Forte, Francesco Paolo Fanizzi, Antonio Mazzocca, Krista Rombouts, Andrea Galli, Vinicio Carloni
Summary: This study reveals that CHK2, a central effector of DNA damage response, regulates cellular energy production by affecting glycolysis and mitochondrial functions. Patients with hepatocellular carcinoma (HCC) showed increased CHK2 mRNA levels in blood, which correlated with elevated TCA metabolites, indicating the role of CHK2 in controlling mitochondrial functions.
Review
Biotechnology & Applied Microbiology
Jiaxue Liu, Boyan Jia, Zhibo Li, Wenliang Li
Summary: Applying reactive polymer materials sensitive to biological stimuli has attracted extensive research interest. The application of reactive oxygen species (ROS)-responsive polymers as drug-delivery systems in organisms has received much attention due to the special physiological effects of ROS on tumors or inflammation. The research progress of ROS-responsive polymers and their application in recent years were summarized and analyzed in this paper. From the perspective of nanoparticle drug delivery systems, multi-responsive delivery systems, and ROS-responsive hydrogels, the future development trends in this field were discussed.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
George Rasti, Maximilian Becker, Berta N. Vazquez, Maria Espinosa-Alcantud, Irene Fernandez-Duran, Andres Gamez-Garcia, Alessandro Ianni, Jessica Gonzalez, Laia Bosch-Presegue, Anna Marazuela-Duque, Anna Guitart-Solanes, Sandra Segura-Bayona, Joan-Josep Bech-Serra, Michael Scher, Lourdes Serrano, Uma Shankavaram, Hediye Erdjument-Bromage, Paul Tempst, Danny Reinberg, Mireia Olivella, Travis H. Stracker, Carolina de la Torre, Alejandro Vaquero
Summary: SIRT1 plays a crucial role in regulating the DNA damage response by interacting with the PP4 phosphatase complex to control the signaling of DNA damage and repair.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Engineering, Environmental
Yizhou Feng, Meiyu Liu, Qiyu Shi, Yunan Song, Luni Yang, Jianfeng Zhang, Zhihua Li, Weihuang Zhu
Summary: In this study, sludge-derived biochar (BS900) was prepared as an effective peroxymonosulfate (PMS) activator, which demonstrated remarkable capability in removing tetracycline (TC). The presence of zero-valent iron (Fe0), elevated Fe2+/Fe3+ ratio, and the increased content of graphitic N were identified as the main factors contributing to the high PMS activation performance of BS900. The study provides a new perspective on the characteristics and mechanism of PMS-based advanced oxidation processes catalyzed by sludge-derived biochar.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2023)
Review
Biochemistry & Molecular Biology
Aikaterini Berdiaki, Monica Neagu, Ioanna Spyridaki, Andrey Kuskov, Serge Perez, Dragana Nikitovic
Summary: Hyaluronan (HA) is a natural compound that is found on the cell surface and tissue extracellular matrix. It is synthesized by enzymes and degraded by other substances. HA has different effects on the body depending on its molecular weight, with high molecular weight HA being anti-inflammatory and low molecular weight HA being pro-inflammatory. Reactive oxygen species (ROS) can degrade HA and affect vascular integrity, while HA can also play a role in wound healing. Understanding the interactions between ROS and HA is an important research topic.
Article
Biochemistry & Molecular Biology
Huan Liu, Wenchao Zhang, Lijie Jin, Shasha Liu, Liying Liang, Yanfei Wei
Summary: This study investigates the anticancer mechanism of Plumbagin (PLB) and its potential connections with oxidative stress, genotoxicity, and cell cycle arrest. The results indicate that PLB significantly inhibits HCC cell viability and colony formation, induces G2/M cell cycle arrest, oxidative stress, and DNA damage. These effects can be attenuated by NAC pretreatment. PLB triggers a DNA damage response by activating ATM, Chk1, Chk2, and p53. Furthermore, the key modulator of the G2/M transition factor, cdc25C, is downregulated in an ROS-dependent manner. Inhibition of the ATM-p53 pathway can reduce the occurrence of G2/M cell cycle arrest. Therefore, ROS-mediated oxidative stress plays a crucial role in PLB-induced G2/M cell cycle arrest mediated by the ATM-p53 pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Jonathan Cowan, Michael R. Longman, Andrew K. Snabaitis
Summary: The study investigates the impact of reduced expression of the alpha 4 protein on cardiomyocytes, showing that alpha 4 knockdown increases DNA damage and cell death, indicating an anti-apoptotic role of alpha 4 in the heart. Additionally, alpha 4 knockdown reduces the expression of anti-apoptotic proteins, increases phosphorylation of p53 and ATM/ATR substrates, and enhances sensitivity to cytotoxic drugs.
SCIENTIFIC REPORTS
(2021)
Article
Pathology
Jennifer Q. J. Zhang, Sayanthooran Saravanabavan, Ashley N. Chandra, Alexandra Munt, Annette T. Y. Wong, Peter C. Harris, David C. H. Harris, Paul McKenzie, Yiping Wang, Gopala K. Rangan
Summary: The study found that DDR signaling is dysregulated in human ADPKD and during the early phases of murine ADPKD, which may promote survival of PKD1-mutated cells and contribute to kidney cyst growth.
AMERICAN JOURNAL OF PATHOLOGY
(2021)
Article
Oncology
Elyse K. Hanly, Neha Y. Tuli, Robert B. Bednarczyk, Robert Suriano, Jan Geliebter, Augustine L. Moscatello, Zbigniew Darzynkiewicz, Raj K. Tiwari
Article
Biochemistry & Molecular Biology
H. Dorota Halicka, Jorge Garcia, Jiangwei Li, Hong Zhao, Zbigniew Darzynkiewicz
Article
Cell Biology
Agnieszka Pierzynska-Mach, Aleksander Szczurek, Francesca Cella Zanacchi, Francesca Pennacchietti, Justyna Drukala, Alberto Diaspro, Christoph Cremer, Zbigniew Darzynkiewicz, Jurek W. Dobrucki
Article
Cell & Tissue Engineering
Maayan Waldman, Lars Bellner, Luca Vanella, Joseph Schragenheim, Komal Sodhi, Shailendra P. Singh, Daohong Lin, Anand Lakhkar, Jiangwei Li, Edith Hochhauser, Michael Arad, Zbigniew Darzynkiewicz, Atallah Kappas, Nader G. Abraham
STEM CELLS AND DEVELOPMENT
(2016)
Article
Oncology
Xiaoxiao Wang, Sufang Zhang, Rong Zheng, Fu Yue, Szu Hua Sharon Lin, Amal A. Rahmeh, Ernest Y. C. Lee, Zhongtao Zhang, Marietta Y. W. T. Lee
Article
Oncology
Monika Podhorecka, Dorota Halicka, Agnieszka Szymczyk, Arkadiusz Macheta, Sylwia Chocholska, Marek Hus, Zbigniew Darzynkiewicz
Article
Oncology
Paulina Rybak, Agnieszka Hoang, Lukasz Bujnowicz, Tytus Bernas, Krzysztof Berniak, Miroslaw Zarebski, Zbigniew Darzynkiewicz, Jerzy Dobrucki
Article
Cell Biology
Laura Garcia-Exposito, Elodie Bournique, Valerie Bergoglio, Arindam Bose, Jonathan Barroso-Gonzalez, Sufang Zhang, Justin L. Roncaioli, Marietta Lee, Callen T. Wallace, Simon C. Watkins, Patricia L. Opresko, Jean-Sebastien Hoffmann, Roderick J. O'Sullivan
Article
Cell & Tissue Engineering
Dariusz Galkowski, Mariusz Z. Ratajczak, Janusz Kocki, Zbigniew Darzynkiewicz
STEM CELL REVIEWS AND REPORTS
(2017)
Article
Cell & Tissue Engineering
Dariusz Galkowski, Mariusz Z. Ratajczak, Janusz Kocki, Zbigniew Darzynkiewicz
STEM CELL REVIEWS AND REPORTS
(2017)
Review
Genetics & Heredity
Marietta Y. W. T. Lee, Xiaoxiao Wang, Sufang Zhang, Zhongtao Zhang, Ernest Y. C. Lee
Article
Oncology
Bhanvi Mishra, Sufang Zhang, Hong Zhao, Zbigniew Darzynkiewicz, Ernest Y. C. Lee, Marietta Y. W. T. Lee, Zhongtao Zhang
CANCER BIOLOGY & THERAPY
(2019)
Article
Genetics & Heredity
Sufang Zhang, Hsiao Hsiang Chao, Xiaoxiao Wang, Zhongtao Zhang, Ernest Y. C. Lee, Marietta Y. W. T. Lee
Review
Genetics & Heredity
Marietta Y. W. T. Lee, Sufang Zhang, Xiaoxiao Wang, Hsiao Hsiang Chao, Hong Zhao, Zbigniew Darzynkiewicz, Zhongtao Zhang, Ernest Y. C. Lee
Article
Multidisciplinary Sciences
Chandana Peddu, Sufang Zhang, Hong Zhao, Agnes Wong, Ernest Y. C. Lee, Marietta Y. W. T. Lee, Zhongtao Zhang