Article
Medicine, General & Internal
Jose Ma. M. Angeles, Atcharaphan Wanlop, Minh-Anh Dang-Trinh, Masashi Kirinoki, Shin-ichiro Kawazu, Aya Yajima
Summary: Asian schistosomiasis caused by Schistosoma mekongi is mainly diagnosed by stool microscopy. However, serodiagnosis using ELISA with soluble egg antigen (SEA) has shown better sensitivity. This study aims to evaluate recombinant S. japonicum antigens for the detection of S. mekongi infection.
Article
Medicine, Research & Experimental
Pei Shen, Tianyu Zhang, Guo Chen, Bei Zhang, Ailong Huang, Lian Duan, Dandan Zhu, Jinling Chen, Jianxin Wang, Yinong Duan
Summary: In this study, the expression of TREM-1 in peritoneal macrophages from mice infected with Schistosoma japonicum was observed. It was found that TREM-1 expression reached its peak at 6 weeks after infection. Furthermore, the study revealed that rSjP40 inhibited TREM-1 expression by down-regulating FOXO3a expression in macrophages.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Immunology
Christelle Kassis, Michelle Durkin, Eric Holbrook, Robert Myers, Lawrence Wheat
Summary: Combining antibody and antigen detection can improve sensitivity and specificity in the diagnosis of coccidioidomycosis, particularly with higher sensitivity in immunocompromised patients. Antigen detection can help prevent missed diagnoses.
CLINICAL INFECTIOUS DISEASES
(2021)
Article
Microbiology
Zhongqiu Mei, Shan Lv, Liguang Tian, Wei Wang, Tiewu Jia
Summary: This study comprehensively evaluated the diagnostic performance of IHA, ELISA, and DDIA for human infections of Schistosoma japonicum in China. DDIA demonstrated the highest pooled sensitivity, while IHA showed the highest pooled specificity. The efficiency and effectiveness of these diagnostic methods vary across different geographical regions.
Article
Chemistry, Analytical
Xinfa Cai, Meijuan Liang, Fei Ma, Sherif Ramzy Mohamed, Amira Abdel Goda, Dawood H. Dawood, Li Yu, Peiwu Li
Summary: A direct competitive nanozyme-linked immunosorbent assay based on MnO2 nanosheets as a nanozyme label was developed for highly sensitive determination of fumonisin B-1. The method showed a wide linear range, low detection limit, high accuracy, and good consistency with the reference method for screening FB1 in cereals.
ANALYTICAL METHODS
(2021)
Article
Immunology
Jie Jiang, Junhui Li, Yu Zhang, Chen Zhou, Chen Guo, Zhaoqin Zhou, Yingzi Ming
Summary: The soluble egg antigen (SEA) of Schistosoma japonicum has been found to suppress rejection and prolong the survival of skin grafts in mouse transplantation models. It achieves this by regulating immune responses, specifically affecting the cellular response to interferon-gamma, IL-17 signaling, chemokine signaling pathways, and cytokine-cytokine receptor interaction. These findings suggest that SEA treatment could be a potential new therapeutic strategy for anti-rejection therapy and inducing tolerance.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Mycology
Diego H. Caceres, Beatriz L. Gomez, Angela M. Tobon, Tom M. Chiller, Mark D. Lindsley
Summary: The sandwich enzyme immunoassay developed by OIDx for Histoplasma urinary Ag showed high sensitivity, but had a high rate of false-positive results in non-histoplasmosis cases.
Article
Medical Laboratory Technology
E. Karakece, M. Koroglu, Huseyin A. Terzi, O. Aydemir, U. Erkorkmaz, Ferhat G. Aslan, M. Altindis
Summary: This study compared the role of the enzyme linked fluorescent assay (ELFA) method with enzyme-linked immunosorbent assay (ELISA) and chemiluminescent microplate immunoassay (CMIA) methods in the diagnosis of HIV infection. It was found that ELFA method had a lower false positive rate and high agreement with confirmation tests, suggesting a potential role in the HIV diagnostic algorithm.
CLINICAL LABORATORY
(2022)
Review
Microbiology
Mateusz Plucinski, Michael Aidoo, Eric Rogier
Summary: The research discusses the significance of identifying and characterizing proteins produced during human infection with Plasmodium spp. in malaria research, diagnosis, and epidemiology. It highlights the development of laboratory methods for protein detection and the potential implications for malaria control strategies.
CLINICAL MICROBIOLOGY REVIEWS
(2021)
Article
Chemistry, Medicinal
Michalis Platis, Dimitrios Vlachakis, Ahmed Ibrahim Foudah, Magdy Mohamed Muharram, Mohamed Hamed Alqarni, Anastassios C. Papageorgiou, Nikolaos E. Labrou
Summary: The study demonstrated that CB3GA is a potent inhibitor of SjGST, providing insights for potential drug design efforts targeting SjGST.
MEDICINAL CHEMISTRY
(2021)
Article
Gastroenterology & Hepatology
Li-Chao Zhang, Xiao-Ying Wu, Rui-Bing Yang, Fang Chen, Jia-Hua Liu, Yun-Yi Hu, Zhong-Dao Wu, Li-Fu Wang, Xi Sun
Summary: IBD affects millions worldwide and lacks therapeutic targets. Studies show that parasitic nematode infections can improve colitis. rSj16 has protective effects on colitis and may involve regulating apoptosis. MiRNA-217-5p could be a potential therapeutic target for IBD.
WORLD JOURNAL OF GASTROENTEROLOGY
(2021)
Article
Nanoscience & Nanotechnology
Wenhao Li, Tiange Gao, Chenxi Lou, Haifang Wang, Yuanfang Liu, Aoneng Cao
Summary: This study presents a novel artificial antibody called Goldbody, which is based on gold nanoparticles and exhibits comparable specificity and improved stability compared to monoclonal antibodies. The Goldbody was successfully utilized in competitive LFIA and ELISA assays for the detection of lysozyme, demonstrating its potential as a substitute for monoclonal antibodies in immunoassays.
ACS APPLIED NANO MATERIALS
(2022)
Article
Chemistry, Analytical
Liping Gao, Yong Li, Zhen-Zhong Huang, Hongliang Tan
Summary: This work demonstrates the potential of stimuli-responsive coordination polymers (CPs) as a host for integrating enzymes to construct a portable immunoassay. By using terbium ion as a metal node and guanine monophosphate as a bridge ligand, an alkaline phosphatase-responsive CPs was fabricated and allowed the integration of an enzyme. The research has been successfully applied in the quantitative analysis of carcinoembryonic antigen.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Xue Liu, Xiuzhai Chen, Xu Zhu, Qing Lin, Xi Pan, Xiaolei Tan, Yongfeng Guo, Jun Qiu, Song Fang
Summary: In this study, ELISA and TRFIA methods were established for the detection and monitoring of quinclorac residue in the environment. The results of both methods were consistent with those of the referenced UPLC-MS/MS method, and they showed good detection performance that met the requirements of quinclorac quantitative determination.
Article
Multidisciplinary Sciences
Kaori Saito, Tomohiko Ai, Akinori Kawai, Jun Matsui, Yoshiyuki Fukushima, Norihiro Kikukawa, Takuya Kyoutou, Masayoshi Chonan, Takeaki Kawakami, Yoshie Hosaka, Shigeki Misawa, Haruhi Takagi, Yasushi Matsushita, Makoto Hiki, Atsushi Okuzawa, Satoshi Hori, Toshio Naito, Takashi Miida, Kazuhisa Takahashi, Yoko Tabe
Summary: The study evaluated the clinical performance of a novel automated immunoassay kit for detecting the nucleocapsid protein of SARS-CoV-2. The kit showed high sensitivity in samples from COVID-19 patients, avoided cross-reactivity with seasonal common cold and influenza coronaviruses, and had high accuracy in detecting samples with low viral load.
SCIENTIFIC REPORTS
(2021)
Article
Medicine, Research & Experimental
Yuxuan Xiong, Zibing Wang, Qiang Wang, Qingyuan Deng, Jitang Chen, Jianshuang Wei, Xiaoquan Yang, Xiangliang Yang, Zifu Li
Summary: This study developed an intelligent nanoplatform that can activate chemodynamic therapy specifically in tumor tissues and provide photoacoustic imaging and amplified combined therapy. The nanoplatform releases therapeutic drugs through interactions with the tumor microenvironment and induces intratumoral oxidative stress. Additionally, the nanoplatform exhibits good biosafety.
Article
Chemistry, Multidisciplinary
Xiaoqiang Zhu, Sihan Chen, Xiuwen Hu, Lijun Zhao, Yiqian Wang, Jinzhao Huang, Jiawen Chen, Yuzhi Qiu, Xuefei Zhang, Mengdie Wang, Xiangliang Yang, Yan Zhang, Yanhong Zhu
Summary: Researchers have developed a bacteria-based cancer immunotherapy using engineered Escherichia coli and lanthanide upconversion nanoparticles. By combining optogenetic tools with the natural behavior of certain bacteria, this novel therapy achieved significant tumor regression with no side effects.
ADVANCED MATERIALS
(2023)
Article
Chemistry, Physical
Yiming Liu, Dingwen Shi, Yanqiao Ren, Ling Li, Yanbing Zhao, Chuansheng Zheng, Xiangliang Yang
Summary: In this study, gold nanoparticles modified temperature sensitive polymer was successfully used to encapsulate doxorubicin and developed into gold nanomedicines. The results showed that these gold nanomedicines exhibited excellent antitumor efficacy and improved the tumor immunosuppressive microenvironment.
Article
Chemistry, Multidisciplinary
Qingqing Zhang, Dingwen Shi, Mengqin Guo, Hao Zhao, Yanbing Zhao, Xiangliang Yang
Summary: Pyroptosis of tumor cells was induced by biGC@PNA with a precisely stoichiometric ratio of Au(I) ion/Au(0) atom through its radiofrequency (RF)-heating effect. The activated pyroptosis resulted in a robust immunogenic cell death (ICD) effect, enhancing the antitumor immune responses and suppressing tumor metastasis and relapse. The noninvasive RF field provides a promising strategy for improving cancer immunotherapy.
Article
Chemistry, Multidisciplinary
Wen Qin, Jinzhao Huang, Chunsheng Yang, Quer Yue, Shizhen Chen, Mengdie Wang, Shangbang Gao, Xin Zhou, Xiangliang Yang, Yan Zhang
Summary: This study presents a novel activatable ferrous nanotheranostics for spatial-temporal control over antitumor ferroptosis responses. The nanotherapeutics can be guided by photoacoustic and magnetic resonance imaging (MRI), and it can effectively induce tumor cell death through photothermal therapy and Fenton reaction. This treatment not only suppresses tumor growth, but also promotes immune response against the tumor.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Engineering, Environmental
Xiaoqiong Zhang, Zhaohan Wei, Ziqiao Ding, Weilin Lv, Jianye Li, Xin Li, Haojie Liu, Panli Yu, Xiangliang Yang, Lu Gan
Summary: Mannose-engineered macrophage-derived microparticles can efficiently repolarize M2-like TAMs enriched with Doxil, improving the immunotherapy and increasing tumor accumulation and penetration of Doxil.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Chemistry, Multidisciplinary
Shinan Geng, Mengqin Guo, Guiting Zhan, Dingwen Shi, Liyun Shi, Lu Gan, Yanbing Zhao, Xiangliang Yang
Summary: A NIR light-triggered dePEGylation/ligand-presenting strategy based on thermal decomposition of azo bonds is developed in this study. It overcomes the PEG dilemma and improves tumor targeting and drug delivery by enhancing cellular uptake and tumor accumulation.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Chemistry, Multidisciplinary
Chong Wang, Qiang Wang, Huimin Wang, Zheng Li, Jitang Chen, Zhijie Zhang, Haowen Zeng, Ximiao Yu, Xiaoquan Yang, Xiangliang Yang, Zifu Li
Summary: This study developed a small molecular prodrug-based nanomedicine with high drug-loading efficiency and tumor selectivity for the treatment of solid tumors, especially triple negative breast cancers. The nanomedicine actively targeted TNBC 4T1 tumor tissues and achieved reduction-responsive drug release. It significantly suppressed tumor growth and eliminated cancer stem cells by disrupting the tumor microenvironment and consuming intracellular glutathione. This research provides valuable insights for the rational design of prodrug nanomedicines for the treatment of stroma- and CSCs-rich solid malignancies.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Chemistry, Physical
Chong Wang, Huimin Wang, Hai Yang, Chen Xu, Qiang Wang, Zheng Li, Zhijie Zhang, Jiankun Guan, Ximiao Yu, Xiaoquan Yang, Xiangliang Yang, Zifu Li
Summary: Researchers designed a nanomedicine that specifically targets cancer-associated fibroblasts (CAFs) in triple-negative breast cancer (TNBC) by conjugating doxorubicin-loaded hydroxyethyl starch-IR780 nanoparticles with a CREKA peptide. The nanomedicine effectively targeted CAFs and generated hyperthermia upon light irradiation, leading to a reduction in CAFs through chemo- and photothermal-therapies. By modulating the tumor mechanical microenvironment (TMME) and eliminating cancer stem cells (CSCs), the growth of the 4T1 tumor was significantly inhibited. This strategy provides a novel therapeutic treatment for desmoplastic solid tumors.
Article
Chemistry, Analytical
Jinzhao Huang, Xuefei Zhang, Shiyu Li, Fei Qu, Biao Huang, Ran Cui, Yijing Liu, Wenbo Hu, Xiangliang Yang, Yan Zhang
Summary: We report a synthesis method for dye-sensitized fluorescent probes, which enhance the fluorescence performance of lanthanide nanoparticles at high doping concentration of Nd3+ by adding indocyanine green (ICG), enabling activatable second near-infrared (NIR-II) fluorescent imaging.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Yuxuan Xiong, Wei Wang, Qingyuan Deng, Zhijie Zhang, Qiang Wang, Zhengtao Yong, Chaoyang Sun, Xiangliang Yang, Zifu Li
Summary: Various PARPi have been approved for the treatment of breast cancers, but they are only effective for HR-deficient breast cancers. The abnormal micro-environment of breast cancers hinders drug delivery. This study used mesoporous polydopamine to deliver PARPi and a DNA-damaging drug, and demonstrated that local mild photothermal therapy could inhibit the HR repair pathway and repress cancer-associated fibroblasts, thereby enhancing the antitumor effect and improving drug delivery.
Article
Pharmacology & Pharmacy
Qiang Wang, Shiyou Li, Chen Xu, Ao Hua, Chong Wang, Yuxuan Xiong, Qingyuan Deng, Xiang Chen, Tian Yang, Jiangling Wan, Ze-yang Ding, Bi-xiang Zhang, Xiangliang Yang, Zifu Li
Summary: In this study, a novel derivative of lonidamine (LND) called HYL001 was found to selectively and potently inhibit cancer stem cells (CSCs) by targeting mitochondria, with significantly lower IC50 values compared to LND. Mechanistically, HYL001 downregulates glutaminase expression to block glutamine metabolism, leading to apoptotic cell death. HYL001 also displays significant antitumor activity in vivo and represses CSCs in fresh tumor tissues from liver cancer patients.
PHARMACOLOGICAL RESEARCH
(2023)
Article
Multidisciplinary Sciences
Zheng Li, Yabo Zhu, Haowen Zeng, Chong Wang, Chen Xu, Qiang Wang, Huimin Wang, Shiyou Li, Jitang Chen, Chen Xiao, Xiangliang Yang, Zifu Li
Summary: The authors designed a series of nanogels with different stiffness and investigated how the mechanical properties of the nanogels can overcome the limitations of RES. Stiff nanogels were able to block uptake capacity of macrophages and temporarily block RES, while soft nanogels efficiently delivered drugs. Combining the advantages of stiff and soft nanogels led to the optimum tumor inhibition effect.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Physical
Xi Tan, Hong Zhou, Chenhui Wang, Xuhan Liu, Xiangliang Yang, Wei Liu
Summary: Combinational chemo-immunotherapy using RNA interference (RNAi) to target PD-L1 has been developed to enhance antitumor immune response. A GSH-responsive camptothecin prodrug-based micellar nanoparticle was designed for the co-delivery of siPD-L1 and CPT, showing high loading efficiency and controlled drug release. This study provides a perspective on the design of co-delivery nanoparticles for antitumor chemo-immunotherapy.
Article
Chemistry, Physical
Yiming Liu, Dingwen Shi, Yanqiao Ren, Ling Li, Yanbing Zhao, Chuansheng Zheng, Xiangliang Yang
Summary: In this study, a temperature sensitive polymer modified with gold nanoparticles was used to encapsulate doxorubicin, resulting in gold nanomedicines with improved antitumor efficacy and enhanced post-operative tumor immunosuppressive microenvironment.
Article
Parasitology
Gabriela Martinez Moura Tavares, Hannar Angelica de Melo Alverga, Estela Moraes Felix, Giovana de Oliveira Dichman, Gabriel Monteiro Pinheiro, Yara Juliano, Priscila Paruci, Dennis Minoru Fujita, Lucas Melo Neves, Marina Tiemi Shio, Luiz Henrique da Silva Nali
Summary: This study investigated the frequency of N. gonorrhoeae infection in women in a poor area of São Paulo and found that most of the strains detected showed resistance to one or more antimicrobials.
Article
Parasitology
Qian Yao, Ying-Ying Fan, Shuang Huang, Gui-Rong Hu, Jun-Ke Song, Xin Yang, Guang-Hui Zhao
Summary: This study found that the expression of miR-4521 was upregulated during C. parvum infection, and this upregulation was related to the TLR/NF-kappa B signal pathway. Additionally, miR-4521 promoted the propagation of C. parvum in HCT-8 cells by regulating BCL2-mediated cell apoptosis through targeting foxm1.
Article
Parasitology
Cho Naing, Maxine A. Whittaker, Wong Siew Tung, Htar Aung, Joon Wah Mak
Summary: This study aimed to estimate the prevalence of zoonotic brugian filariasis in animals and compare it with the prevalence in humans in the same area. The findings suggest that cats, dogs, and leaf-eating monkeys may serve as potential reservoirs for human brugian filariasis.
Review
Parasitology
Raissa Nogueira de Brito, Susan Tanner, Julie Velasquez Runk, Juliana Hoyos
Summary: This study conducted a scoping review to identify major research themes and knowledge gaps in the social science literature on leishmaniases or Chagas disease. The findings showed that research in this field mainly focuses on the sociocultural, economic, historical, and political dimensions of the diseases. Future research should pay more attention to how social institutions and economic factors shape disease education, control measures, healthcare access, and quality of life of affected individuals.
Article
Parasitology
Minghao Zheng, Mengyao Zhang, Hao Li, Shipo Wu, Yuee Zhao, Jinlong Zhang, Yunyue Zhou, Mohamed Boie Jalloh, Kun Zhang, Lina Chen, Zhiqiang Mi, Yong Cui, Lihua Hou
Summary: Rapid and convenient detection of Plasmodium falciparum, the deadliest malaria parasite in Africa, is crucial for malaria eradication efforts in medical resource-limited settings. A CRISPR-based diagnostic method that can detect P. falciparum without nucleic acid extraction was developed and showed high sensitivity and consistency with qPCR test. It can also detect artemisinin-resistant strains and other laboratory-cultured strains. This method has great potential for clinical testing and large-scale community screening in Africa and other regions.
Article
Parasitology
Fabian Correa-Morales, Cassandra Gonzalez-Acosta, David Ibarra-Ojeda, Miguel Moreno-Garcia
Summary: Since 2002, West Nile Virus (WNV) has been reported in 18 states in Mexico. This paper discusses the proposed hypotheses for the absence of WNV outbreaks in Latin America and provides a context-specific explanation for Mexico.
Article
Parasitology
Umberto Molini, Lauren M. Coetzee, Vernon Christians, Maria Y. Hemberger, Bernard Chiwome, Maria Amukwaya, Siegfried Khaiseb, Giovanni Cattoli, William G. Dundon, Giovanni Franzo
Summary: Porcine circovirus 3 (PCV-3) has been reported globally since its first identification in 2015. Limited data on PCV-3 in Africa led to a study in Namibia, which found a high prevalence of PCV-3 in backyard piggeries, but not in commercial farms with higher biosecurity measures. The source of viral introduction and factors such as semen importation, feeding backyard pigs with human food waste, pig exchanges, and wildlife movements may be involved in the dispersal of PCV-3 within Namibia. Further studies are needed to understand the epidemiology and impact of PCV-3 on pig productivity and wildlife health.
Article
Parasitology
Chutchai Piewbang, Panida Poonsin, Pattiya Lohavicharn, Panitnan Punyathi, Sawang Kesdangsakonwut, Tanit Kasantikul, Somporn Techangamsuwan
Summary: This study provides insights into the pathology of natural SARS-CoV-2 infection in dogs, revealing severe damage to the lungs and involvement of multiple organs. The findings suggest the potential utility of dogs as a model for studying SARS-CoV-2 infection in humans, highlighting the need for further investigation.
Article
Parasitology
Julian Avila-Jimenez, Juan David Gutierrez, Mariano Altamiranda-Saavedra
Summary: Leishmaniasis is a parasitic disease transmitted to humans by sandfly vectors and vertebrate hosts. This study used ecological niche modeling to investigate the potential distribution of host and vector species associated with cutaneous leishmaniasis transmission, revealing different distribution patterns under varying climatic conditions.
Article
Parasitology
Luis A. Gomez-Puerta, Juan Olazabal, Santiago Paredes, Juan Lopez, Miguel Anampa, Angelica Mondragon, Ana Vargas-Calla, Hugo Castillo
Summary: This study reports the first detection of Candidatus M. haemolamae infection in vicunas from the Pampa Galeras National Reserve in Peru, using molecular diagnosis. Tick-infested vicunas had a higher likelihood of being infected with Candidatus M. haemolamae compared to tick-free vicunas. Gender and age were not associated with infection.
Article
Parasitology
Berlin Londono-Renteria, Zakaria Seidu, Helena Lamptey, Michael F. Ofori, Lars Hviid, Mary Lopez-Perez
Summary: Heterozygous carriers of HbAS and HbAC have a reduced risk of severe malaria, but not immunity against Plasmodium falciparum infection. Antibody levels against gSG6-P1 peptide in Anopheles mosquito saliva can serve as a serological biomarker for exposure to Plasmodium infection. This study highlights the importance of studying the role of hemoglobinopathies in malaria transmission for improving control interventions.
Article
Parasitology
Imane Belbacha, Meryem El Azzouzi, Rajae Bensghir, Kamal Filali Marhoum, Khadija Hajjout, El Mir Elharti, Khalid Sadki, Hicham Oumzil
Summary: This study investigates the association of three SNPs in the APOBEC3G gene with disease outcomes in Moroccan HIV-1 infected patients. The rs2294367 CG genotype is strongly associated with protection against HIV-1 infection, particularly in older individuals.
Article
Parasitology
Tan Luong, Do Kien Tran, Anh Hung Pham, Thi Thu-Ha Hoang, Van Khang Pham, Quang Thai Pham, Thi Mai Hung Tran, Minh Hoa Luong, Thanh Long Pham, Jason K. Blackburn
Summary: This study examined the epidemiology of anthrax in Lai Chau province, Vietnam, using historical data. Spatial clusters of human and livestock anthrax were identified, and the characteristics of the disease were described. The study found that there were distinct differences between the high-risk areas for human and livestock anthrax, and most human cases occurred during specific seasons. Additionally, the decrease in livestock anthrax vaccine coverage was associated with an increase in disease incidence.
Article
Parasitology
Jin-tao Chen, Jian-bo Zhan, Ming-chao Zhu, Kai-ji Li, Man-qing Liu, Bin Hu, Kun Cai, Hai-rong Xiong, Shu-liang Chen, Wei-long Tan, Liang-jun Chen, Wei Hou
Summary: This study investigated the prevalence and variation of orthohantavirus in rodents and humans in Hubei province, China. It identified the diversity of orthohantaviruses circulating in the region, with the HV004-like subtype being the main genotype in rodents and patients. This highlights the importance of continued attention and research on orthohantaviruses, especially newly identified strains.
