Review
Biochemistry & Molecular Biology
Mathilde Fenelon, Paul Galvez, Daniel Kalbermatten, Paolo Scolozzi, Srinivas Madduri
Summary: The amniotic membrane (AM) is a widely available and cost-effective tissue with unique biological properties. Advanced hybrid materials based on AMs have been developed by combining them with secondary materials. This review focuses on the increasing development of new multilayer composite-based AMs and highlights the benefits of additive manufacturing technologies and electrospinning in expanding their use for tissue engineering and clinical applications. The use of AMs and multilayer composite-based AMs in nerve regeneration is particularly emphasized.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Materials Science, Multidisciplinary
Jing Wang, Chunxiang Feng, Yunpeng Zhu, Zhixian Wang, Xiang Ren, Xing Li, Yisheng Ying, Yiqun Tian, Kai Yu, Shiliang Liu, Chang Liu, Xiaoyong Zeng
Summary: Bladder tissue engineering using adipose tissue extract (ATE) shows potential for promoting pluripotent marker proteins in stem cells and enhancing proliferation, migration, and angiogenesis in endothelial cells. The integration of electrospun nanofibers and hyaluronic acid colloidal solution in a patch demonstrates feasibility and effectiveness in repairing bladder defects in a rat model, highlighting its potential for tissue engineering and regenerative medicine.
MATERIALS & DESIGN
(2022)
Article
Biochemistry & Molecular Biology
Katharina Peters, Nadine Wiesmann, Diana Heimes, Roxana Schwab, Peer W. Kammerer, Bilal Al-Nawas, Ronald E. Unger, Annette Hasenburg, Walburgis Brenner
Summary: Oral mucosa equivalents can be used as grafts for tissue reconstruction. Extracorporeal shock wave therapy (ESWT) has been shown to stimulate the formation and differentiation of epithelium in vitro, leading to a more distinct and differentiated multilayered epithelium and potentially improving the function of tissue equivalents.
Article
Nanoscience & Nanotechnology
Abdel H. Mahmoud, Yuanyuan Han, Renan Dal-Fabbro, Arwa Daghrery, Jinping Xu, Darnell Kaigler, Sarit B. Bhaduri, Jos Malda, Marco C. Bottino
Summary: In this study, highly innovative nanoscale beta-tricalcium phosphate (β-TCP)-laden gelatin methacryloyl/polycaprolactone (GelMA/PCL-TCP) photocrosslinkable composite fibrous membranes were fabricated via electrospinning for guided bone regeneration (GBR). The composite microfibers showed a uniform porous network and successful integration of β-TCP particles. The GelMA/PCL-TCP membranes exhibited enhanced cell attachment, proliferation, mineralization, and osteogenic gene expression in alveolar bone-derived mesenchymal stem cells (aBMSCs) compared to pure PCL and GelMA/PCL. Furthermore, the GelMA/PCL-TCP membrane promoted robust bone regeneration in rat calvarial critical-size defects, demonstrating remarkable osteogenesis compared to PCL and GelMA/PCL groups. Overall, the electrospun GelMA/PCL-TCP composite showed great potential as a promising membrane for guided bone regeneration.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Engineering, Biomedical
Elrade Rofaani, Yong He, Juan Peng, Yong Chen
Summary: This study developed a method to overcome the lack of in vivo-like basement membrane (BM) for in vitro modeling by culturing primordial lung progenitors (PLPs) on an artificial basement membrane (ABM) composed of collagen IV and laminin. The forces generated by the growing epithelial monolayer on the ABM led to geometry-dependent folding and enhanced expression of surfactant protein C and aquaporin 5 compared with a nanofiber-covered substrate. This work emphasizes the importance of recapitulating natural BM for advanced epithelial modeling.
ACTA BIOMATERIALIA
(2023)
Article
Engineering, Biomedical
Minglei Yang, Jiafei Chen, Yi Chen, Weikang Lin, Hai Tang, Ziwen Fan, Long Wang, Yunlang She, Feng Jin, Lei Zhang, Weiyan Sun, Chang Chen
Summary: A scaffold-free modular strategy for tracheal engineering is developed, which constructs a complex tracheal substitute by laminating a cartilage sheet and a temperature-sensitive epithelium sheet. This substitute exhibits native-like structure and function, showing great clinical potential for tracheal reconstruction.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Biotechnology & Applied Microbiology
Yuqing Niu, Massimiliano Galluzzi, Fuming Deng, Zhang Zhao, Ming Fu, Liang Su, Weitang Sun, Wei Jia, Huimin Xia
Summary: The study found that the hyaluronic acid-coating silk fibroin nanofiber scaffold can effectively regulate the epithelialization process of urethral regeneration, promoting luminal epithelialization, smooth muscle bundle structural remodeling, and capillary formation.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2022)
Article
Engineering, Biomedical
Yubo Shi, Lei Wang, Liguo Sun, Zhennan Qiu, Xiaoli Qu, Jingyi Dang, Zhao Zhang, Jiankang He, Hongbin Fan
Summary: Melt electrospinning writing (MEW) is a promising 3D printing technology for scaffold fabrication. This study investigated the use of NaOH treatment to enhance the cytocompatibility and osteoinductive properties of polycaprolactone (PCL) scaffolds. The NaOH-treated PCL scaffold exhibited nanopits and nanogrooves on the surface, which increased surface roughness and wettability, leading to improved cell proliferation and adhesion. In vitro experiments demonstrated that the NaOH-treated surface could induce osteogenic differentiation of bone marrow mesenchymal stem cells via the integrin alpha 2/beta 1-PI3K-Akt signaling pathway. Animal studies showed that the NaOH-treated PCL scaffold promoted new bone formation. The study concluded that NaOH treatment is a simple and effective method to enhance the cellular affinity and osteoinductive property of MEW PCL scaffolds.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2023)
Article
Biotechnology & Applied Microbiology
Allison E. Fetz, Shannon E. Wallace, Gary L. Bowlin
Summary: Chloroquine was found to suppress the release of NETs by neutrophils in the early stages, while also modulating the secretion of signaling molecules, potentially aiding in tissue regeneration and offering immunomodulatory effects.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Elrade Rofaani, Boxin Huang, Feng Liang, Juan Peng, Yong Chen
Summary: This study successfully generated airway epithelium using artificial basement membrane (ABM) and human-induced pluripotent stem cells (hiPSCs). The generated pseudostratified airway epithelium consists of multiple cell types and demonstrates advantages over other methods.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Review
Biochemistry & Molecular Biology
Nargess Khalilgharibi, Yanlan Mao
Summary: The basement membrane plays a crucial role in tissue development and diseases, with its complex mechanical properties changing through different molecular mechanisms, thereby affecting tissue form and function. Investigating the mechanical properties of the basement membrane is important for disease diagnosis and treatment, as well as the development of better in silico and in vitro models, which can also reduce the use of animals in research.
Article
Engineering, Environmental
Bing Wang, Tianwen Xin, Lang Shen, Kun Zhang, Dan Zhang, Hui Zhang, Jisheng Liu, Bing Chen, Wenguo Cui, Yilai Shu
Summary: The GelMA-TA double-cross-linked hydrogel acoustic transmitted electrospun fibrous membrane, constructed with Tannic acid and Gelatin methacryloyl, shows excellent acoustic effects and mechanical properties, promoting tympanic membrane regeneration and facilitating faster healing to form a structure similar to the natural TM. By adjusting the concentration of Tannic acid, the sound response sensitivity can be improved and sound loss reduced.
CHEMICAL ENGINEERING JOURNAL
(2021)
Article
Ophthalmology
Aruni K. Makuloluwa, Kevin J. Hamill, Saaeha Rauz, Lucy Bosworth, Atikah Haneef, Vito Romano, Rachel L. Williams, Darlene A. Dartt, Stephen B. Kaye
Summary: The conjunctiva, as the largest component of the ocular surface, may require grafts for restoration of function in cases of damage. Studies on biological and synthetic substrates for ex vivo culture of conjunctival epithelial cells have been conducted. This review discusses various types of substrates used for conjunctival transplantation with a focus on those based on the extracellular matrix.
Article
Ophthalmology
Aruni K. Makuloluwa, Kevin J. Hamill, Saaeha Rauz, Lucy Bosworth, Atikah Haneef, Vito Romano, Rachel L. Williams, Darlene A. Dartt, Stephen B. Kaye
Summary: The conjunctiva can be damaged by various diseases, requiring conjunctival grafts for restoration of function. Research on ex vivo culture and improvement of substrates is important for tissue grafts. Understanding the composition and structure of conjunctival extracellular matrix (ECM) is crucial for developing better biological substrates.
Review
Nanoscience & Nanotechnology
Ping Zhao, Wei Chen, Zhangbin Feng, Yukang Liu, Ping Liu, Yufeng Xie, Deng-Guang Yu
Summary: This article introduces the research progress and application of electrospun nanofibrous membranes for periodontitis treatment. Electrospun nanofibrous membranes have a large specific surface area and high porosity, which can improve the absorption capacity of proteins, promote the expression of specific genes, improve cell adhesion properties, and prevent fibroblasts from passing through the barrier membrane. Currently, various nanofibrous membranes with antibacterial, anti-inflammatory, and tissue regeneration properties have been prepared for periodontal treatment. Electrospinning has unique advantages in periodontal treatment and has potential for further development in the future.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2022)