Review
Biochemistry & Molecular Biology
Yoshifumi Takahata, Hiromasa Hagino, Ayaka Kimura, Mitsuki Urushizaki, Shiori Yamamoto, Kanta Wakamori, Tomohiko Murakami, Kenji Hata, Riko Nishimura
Summary: This review article summarizes the research progress on the functions and regulatory mechanisms of lubricin and growth differentiation factor 5 (Gdf5) in osteoarthritis (OA), as well as their roles in maintaining articular cartilage homeostasis and the development of OA. Molecular targeting of Prg4 and Gdf5 is expected to provide new insights into the etiology, pathogenesis, and potential treatment of OA.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Mi Ra Chang, Patrick R. Griffin
Summary: Osteoarthritis (OA) is a common joint disease that affects the elderly population and is a major cause of disability and impaired mobility. Current treatments focus on pain relief and reducing joint swelling, but there are no effective methods to slow disease progression and prevent cartilage loss. Research suggests that ROR beta plays a critical role in chondrogenesis and identifying mechanisms that control its expression could lead to the development of new therapies for treating OA.
Article
Cell Biology
Anais Defois, Nina Bon, Alexandre Charpentier, Melina Georget, Nicolas Gaigeard, Frederic Blanchard, Antoine Hamel, Denis Waast, Jean Armengaud, Ophelie Renoult, Claire Pecqueur, Yves Maugars, Marie-Astrid Boutet, Jerome Guicheux, Claire Vinatier
Summary: This study reveals a strong association between inflammation and metabolic dysregulation in osteoarthritic chondrocytes. Inflammation affects metabolic-related genes and leads to increased glycolysis and decreased mitochondrial respiration in osteoarthritic chondrocytes. These findings provide important insights for further understanding the mechanisms of osteoarthritis.
CELL COMMUNICATION AND SIGNALING
(2023)
Article
Biochemistry & Molecular Biology
Elena Della Morte, Stefania Niada, Chiara Giannasi, Luigi Zagra, Anna Teresa Brini
Summary: This study found that TNF-α can rapidly induce a decrease in Cx43 expression in articular chondrocytes, mainly through the proteasomal pathway. The proteasome inhibitor MG132 and the synthetic factor TGF-β 1 can reverse this decrease. These results suggest a potentially important role of Cx43 perturbation in joint inflammation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Kohei Yamaura, Nicholas A. Sather, Anna Metlushko, Haruki Nishimura, Radoslav Z. Pavlovic, Sealy Hambright, Sudheer K. Ravuri, Marc J. Philippon, Samuel I. Stupp, Chelsea S. Bahney, Johnny Huard
Summary: This study designed an injectable peptide amphiphile (PA) nanofiber that can provide sustained and local losartan release, promoting cartilage regeneration. The results showed that the positively-charged nanofibers not only promoted cell proliferation but also significantly increased the production of glycosaminoglycans in cartilage. Moreover, gene expression analysis suggested that losartan-encapsulated nanofibers had significant anti-inflammatory, anti-degenerative, and cartilage regenerative effects by significantly blocking TGF-beta 1 in this in vitro system.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Hong-Xing Liao, Zhi-Hui Zhang, Hui-Lin Chen, Ying-Mei Huang, Zhan-Liang Liu, Jian Huang
Summary: A novel HA-related circRNA, circHYBID, was identified as a potential regulator of HA metabolism in chondrocytes, promoting HA synthesis and accumulation. The circHYBID-hsa-miR-29b-3p-TGF-beta 1 axis plays a crucial role in cartilage inflammation and OA progression, suggesting circHYBID as a promising target for OA therapy.
MOLECULAR MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Zhicheng Yang, Wei Jiang, Chenwei Xiong, Jingjing Shang, Yong Huang, Xindie Zhou, Su Zhang
Summary: Globally, tens of millions of individuals suffer from osteoarthritis (OA), a degenerative joint condition without a definitive cure. This study investigated the molecular mechanisms by which calcipotriol alleviates chondrocyte ferroptosis, a key process in OA. The results showed that calcipotriol can inhibit chondrocyte GPX4-mediated ferroptosis by suppressing the expression of transforming growth factor-beta 1, and intra-articular calcipotriol injection significantly reduces cartilage degradation in rats with OA. These findings suggest that calcipotriol may have a potential therapeutic effect on OA.
Article
Cell Biology
Arijita Sarkar, Nancy Q. Q. Liu, Jenny Magallanes, Jade Tassey, Siyoung Lee, Ruzanna Shkhyan, Youngjoo Lee, Jinxiu Lu, Yuxin Ouyang, Hanhan Tang, Fangzhou Bian, Litao Tao, Neil Segil, Jason Ernst, Karen Lyons, Steve Horvath, Denis Evseenko
Summary: This study establishes an epigenetic clock based on DNA methylation profiling of human chondrocytes and reveals an association between CpG methylation and chondrocyte age. It also demonstrates the role of STAT3 in regulating DNA methylation in chondrocyte development and osteoarthritis. Furthermore, functional assessment shows that a subset of chondrocytes acquire a progenitor-like phenotype.
Article
Cell Biology
Nathalie G. M. Thielen, Margot Neefjes, Elly L. Vitters, Henk M. van Beuningen, Arjen B. Blom, Marije I. Koenders, Peter L. E. M. van Lent, Fons A. J. van de Loo, Esmeralda N. Blaney Davidson, Arjan P. M. van Caam, Peter M. van der Kraan
Summary: During osteoarthritis, TGF-beta signaling pathways contribute to the hypertrophy-like phenotype in chondrocytes. This study found that ALK5 kinase activity is essential for the induction of crucial hypertrophy factors in TGF-beta-stimulated chondrocytes.
Article
Immunology
Junlai Wan, Ziqing Zhu, Zhiyi He, Hua Wu, Anmin Chen, Wentao Zhu, Peng Cheng
Summary: Stevioside (SVS) might alleviate cartilage degradation in mice with osteoarthritis (OA) by inhibiting the expression of inflammatory cytokines, reducing cell apoptosis and improving autophagy, as well as blocking the activation of signaling pathways. In vivo experiments demonstrated the potential therapeutic effect of SVS on OA.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Orthopedics
Earnest L. Taylor, John A. Collins, Pryia Gopalakrishnan, Susan Chubinskaya, Richard F. Loeser
Summary: This study investigated the effect of age and oxidative stress on the regulation of Nrf2 in young, old, and osteoarthritic human articular chondrocytes. The levels of Nrf2, antioxidant proteins, and anabolic response were evaluated, and it was found that Nrf2 protein levels were significantly lower in older adult and OA chondrocytes compared to younger cells. The findings suggest that maintaining Nrf2 activity could be useful in maintaining chondrocyte homeostasis.
OSTEOARTHRITIS AND CARTILAGE
(2023)
Article
Biochemistry & Molecular Biology
Masahiro Horita, Shun-Neng Hsu, Anna Raper, Colin Farquharson, Louise A. Stephen
Summary: The study found that miR-29b-3p plays an inhibitory role in the downregulation induced by TGF-beta 1, thereby affecting the cell cycle distribution of murine articular chondrocytes, suggesting that miR-29b-3p may contribute to reduced chondrocyte proliferation in OA.
BIOCHEMISTRY AND BIOPHYSICS REPORTS
(2022)
Article
Cell Biology
Xinmei Du, Mengmeng Duan, Shiyi Kan, Yueyi Yang, Siqun Xu, Jieya Wei, Jiazhou Li, Hao Chen, Xuedong Zhou, Jing Xie
Summary: Mitochondrial dynamics plays a crucial role in cartilage physiology, and perturbations in this process can lead to cartilage diseases. This study investigates the impact of TGF-beta 3, a growth factor present in OA joints, on mitochondrial dynamics in chondrocytes. The findings reveal that TGF-beta 3 increases the number of mitochondria by promoting their fission through AMPK signaling. This study enhances our understanding of the role of TGF-beta 3 in mitochondrial dynamics and offers potential therapeutic strategies for cartilage injury and OA disease.
CELL PROLIFERATION
(2023)
Article
Biochemistry & Molecular Biology
Kanyakorn Riewruja, Suphattra Phakham, Patlapa Sompolpong, Rangsima Reantragoon, Aree Tanavalee, Srihatach Ngarmukos, Wanvisa Udomsinprasert, Tanyawan Suantawee, Sinsuda Dechsupa, Sittisak Honsawek
Summary: This study examined the cytokine profile in PRP and PPP of knee OA patients and investigated the effects of PRP on OA chondrocytes and knee OA patients. The results showed that PRP contained a wide variety of cytokines, chemokines, and growth factors, and intra-articular PRP injection had favorable outcomes in knee OA patients. The findings suggest that PRP treatment may be a potential therapeutic strategy for alleviating knee pain and delaying the need for surgery.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Xiangguo Che, Xian Jin, Na Rae Park, Hee-June Kim, Hee-Soo Kyung, Hyun-Ju Kim, Jane B. Lian, Janet L. Stein, Gary S. Stein, Je-Yong Choi
Summary: TGF-beta signaling is regulated by Cbf beta, which plays a vital role in maintaining articular cartilage homeostasis. Deficiency of Cbfb leads to cartilage degeneration and enhances osteoarthritis progression. Cbf beta protects Runx1 from degradation and regulates the expression of Type II collagen, indicating its importance in cartilage integrity and TGF-beta signaling.
Article
Genetics & Heredity
Richard M. Silverman, Erin E. Cummings, Linda P. O'Reilly, Mark T. Miedel, Gary A. Silverman, Cliff J. Luke, David H. Perlmutter, Stephen C. Pak
Article
Multidisciplinary Sciences
Erin E. Cummings, Linda P. O'Reilly, Dale E. King, Richard M. Silverman, Mark T. Miedel, Cliff J. Luke, David H. Perlmutter, Gary A. Silverman, Stephen C. Pak
Editorial Material
Orthopedics
Richard M. Silverman, Matthew J. Matava
Summary: PROMIS, funded by NIH, enhances measurement of musculoskeletal function and predicts postoperative outcomes for patients undergoing arthroscopic meniscectomy. PROMIS scores can be used by surgeons to counsel patients considering the surgery.
ARTHROSCOPY-THE JOURNAL OF ARTHROSCOPIC AND RELATED SURGERY
(2021)
Article
Orthopedics
Robert H. Brophy, Richard M. Silverman, Kent Jason Lowry
Summary: The American Academy of Orthopaedic Surgeons (AAOS) has released a clinical practice guideline in 2022 to provide optimal management for anterior cruciate ligament (ACL) tears. The guideline was developed with input from various medical societies and is based on the best available evidence. The cases presented in this article serve as examples to demonstrate the clinical application of these guidelines.
JOURNAL OF THE AMERICAN ACADEMY OF ORTHOPAEDIC SURGEONS
(2023)