Article
Plant Sciences
Qiang Lu, Zhenshan Zhang, Yifei Xu, Yujia Chen, Cailan Li
Summary: This study aimed to investigate the inhibitory effect and mechanism of Sanguinarine (a natural alkaloid from Zanthoxylum nitidum) on Helicobacter pylori urease (HPU) and jack bean urease (JBU). The results showed that Sanguinarine significantly suppressed the activities of HPU and JBU, and the mechanism involved thiol groups and Ni2+.
JOURNAL OF ETHNOPHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Hyun Jun Woo, Ji Yeong Yang, Pyeongjae Lee, Jong-Bae Kim, Sa-Hyun Kim
Summary: The natural compound zerumbone extracted from Zingiber zerumbet Smith was found to inhibit the urease activity of H. pylori by forming dimers, trimers, or tetramers, suggesting its potential as a therapeutic agent for treating H. pylori infections in humans.
Article
Medicine, General & Internal
Masafumi Nishino, Toshihiko Kakiuchi, Kayoko Fukuda, Masato Yoshiura
Summary: The urea breath test (UBT) is commonly used for diagnosing and eradicating Helicobacter pylori infection, but it can also produce positive results for urease-active bacteria other than H. pylori. This case study reports a 15-year-old boy who was diagnosed with H. pylori infection through UBT, but continue to have positive results even after treatment. Further tests indicated the presence of oral commensal bacteria with urease activity.
FRONTIERS IN MEDICINE
(2023)
Article
Infectious Diseases
Tomomi Kobatake, Keiki Ogino, Hiroyuki Sakae, Kazuyoshi Gotoh, Akari Watanabe, Osamu Matsushita, Hiroyuki Okada, Kenji Yokota
Summary: The study found that disulfiram has bactericidal effects on Helicobacter pylori at low concentrations, suggesting it can serve as a supplement to current H. pylori eradication drugs.
INFECTION AND DRUG RESISTANCE
(2021)
Review
Microbiology
Shamshul Ansari, Yoshio Yamaoka
Summary: Despite the decrease in H. pylori infection rates, gastric cancer morbidity and mortality rates remain high, fueled by antimicrobial resistance and treatment failure. Accurate diagnosis is crucial for effective treatment and eradication of infections. This article provides a comprehensive description of available diagnostic methods, aiding clinicians and researchers in selecting appropriate approaches and emphasizing the importance of clinical settings, patient factors, and clinical factors in diagnosis and evaluating eradication efficacy.
CLINICAL MICROBIOLOGY REVIEWS
(2022)
Article
Chemistry, Physical
Yafei Liu, Huixia Feng, Luyao Wang, Tiantian Yang, Jianhui Qiu
Summary: A corrosion inhibitor for the bis-thiophene Schiff base copper-metal complex Cu(II)@Thy-2 was investigated, which effectively reduces the self-corrosion current density and improves the corrosion resistance. After adding Cu(II)@Thy-2 corrosion inhibitor, a uniformly distributed dense corrosion inhibitor adsorption film formed on the metal surface, significantly improving its corrosion resistance. The adsorbed inhibitor film decreased the metal surface's hydrophilicity and increased its hydrophobicity.
Article
Oncology
Michael J. Maroney, Stefano Ciurli
Summary: Helicobacter pylori, a human pathogen causing peptic ulcers and classified as a Class I carcinogen, relies on nickel-urease and hydrogenase enzymes for survival and virulence. Nickel plays a crucial role in virulence, and proteins involved in nickel trafficking may be potential antibiotic targets.
SEMINARS IN CANCER BIOLOGY
(2021)
Article
Microbiology
Tran Thi Dieu Thuy, Pei-Yun Kuo, Sha-Ma Lin, Cheng-Yen Kao
Summary: This study evaluated and compared the anti-Helicobacter pylori activity of three potential probiotic strains and found that SLC13 showed the highest adhesion ability and inhibitory activity. It also alleviated the inflammatory response induced by H. pylori infection.
Article
Medicine, General & Internal
Natalia Garcia-Morales, Angeles Perez-Aisa, Giulia Fiorini, Bojan Tepes, Manuel Castro-Fernandez, Alfredo Lucendo, Irina Voynovan, Luis Bujanda, Ana Garre, Luis Rodrigo, Samuel Jesus Martinez Dominguez, Maja Denkovski, Jose M. Huguet Malaves, Laimas Jonaitis, Renate Bumane, Oleg Zaytsev, Pilar Mata Romero, Jesus Barrio, Luis Fernandez-Salazar, Aiman Silkanovna Sarsenbaeva, Inmaculada Ortiz Polo, Sergey Alekseenko, Ilaria Maria Saracino, Dino Vaira, Alma Keco-Huerga, Dmitry Bordin, Antonio Gasbarrini, Frode Lerang, Theodore Rokkas, Juozas Kupcinskas, Marcis Leja, Gulustan Babayeva, Ricardo Marcos Pinto, Ante Tonkic, Sinead Smith, Perminder Phull, Gyorgy Buzas, Halis Simsek, Doron Boltin, Oleksiy Gridnyev, Marino Venerito, Vladimir Milivojevic, Nuria Tora, Anna Cano-Catala, Leticia Moreira, Olga P. Nyssen, Francis Megraud, Colm O'Morain, Javier P. Gisbert, Ignasi Puig
Summary: This study evaluates the tests used for diagnosing Helicobacter pylori infection in Europe, including initial diagnosis and confirmation of eradication. The results show a great heterogeneity in the tests used among different countries, indicating a need to further explore the reasons for the lack of adherence to clinical guidelines.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Chemistry, Multidisciplinary
Se Yun Jeong, Myung Woo Na, Eon Chung Park, Jin-Chul Kim, Dong-Min Kang, Hamed Hamishehkar, Mi-Jeong Ahn, Jung Kyu Kim, Ki Hyun Kim
Summary: In this study, a phytochemical analysis of Pinus eldarica needles collected in Iran was conducted, resulting in the isolation and identification of three labdane-type diterpenes, including a new norlabdane-type diterpene. These diterpenes exhibited antibacterial activity against Helicobacter pylori, highlighting their potential as novel antibiotics for the treatment of gastric and duodenal ulcers.
Article
Chemistry, Multidisciplinary
Dong-Min Kang, Atif Ali Khan Khalil, Woo Sung Park, Hye-Jin Kim, Kazi-Marjahan Akter, Ji-Yeong Bae, Sultan Mehtap Buyuker, Jung-Hwan Kim, Kwon Kyoo Kang, Mi-Jeong Ahn
Summary: This study isolated six compounds from Rumex acetosa that showed anti-H. pylori activity, making them potential natural candidates for the treatment of H. pylori infection. Two of the compounds exhibited high antibacterial activity against H. pylori strains.
Review
Medicine, General & Internal
Dmitry S. Bordin, Irina N. Voynovan, Dmitrii N. Andreev, Igor Maev
Summary: This article reviews the various methods available for detecting Helicobacter pylori, including both invasive and non-invasive diagnostic tests, highlights the advancements and new developments in endoscopic examination techniques, and discusses the advantages and limitations of different diagnostic methods and their applications.
Article
Medicine, General & Internal
Peter Malfertheiner, M. Constanza Camargo, Emad El-Omar, Jyh-Ming Liou, Richard Peek, Christian Schulz, Stella I. Smith, Sebastian Suerbaum
Summary: Helicobacter pylori infection causes chronic gastritis and can lead to severe gastroduodenal pathologies, including peptic ulcer and gastric cancer. This Primer summarizes the epidemiology, pathophysiology, diagnosis and management of this infection, and discusses patient quality of life and open research questions.
NATURE REVIEWS DISEASE PRIMERS
(2023)
Article
Agronomy
Fernando Torralbo, Dara Boardman, James H. Houx, Felix B. Fritschi
Summary: This study compared the effectiveness of different enhanced efficiency fertilizer (EEF) urea technologies on maize yield and ammonia losses. The results showed that polymer-coated urea effectively reduced ammonia losses and maintained or increased maize yield.
Article
Food Science & Technology
Chrysoula (Chrysa) Voidarou, Georgios Rozos, Athanasios Alexopoulos, Stavros Plessas, Ioanna Mantzourani, Elisavet Stavropoulou, Athina Tzora, Eugenia Bezirtzoglou
Summary: Oregano honey, produced by bees in oregano fields of alpine altitudes at the mountainous area of Epirus, Greece, showed potential therapeutic properties against gastric ulcers and gastritis, particularly against Helicobacter pylori strains. The honey demonstrated significant inhibitory activity against urease secreted by the pathogen, suggesting multiple modes of action for its antibacterial effects. Furthermore, different organic solvent extracts of oregano honey exhibited potent antibacterial action, with N-hexane and chloroform extracts being the most effective.
Article
Chemistry, Organic
Wellington Alves de Barros, Marcelo Pereira Queiroz, Leonardo da Silva Neto, Graziele Martins Borges, Felipe Terra Martins, Angelo de Fatima
Summary: An expeditious method for the synthesis of NBOHs and NBOMes from salicylaldehyde and 2-methoxyaldehyde was reported, with overall yields ranging from 7% to 38%. The X-ray structures of the synthesized compounds were determined, showing potential applications in pharmacology and forensics.
TETRAHEDRON LETTERS
(2021)
Article
Multidisciplinary Sciences
Leonardo da Silva Neto, Angelica Faleiros da Silva Maia, Adriana Martins Godin, Paulo Sergio de Almeida Augusto, Raissa Lima Goncalves Pereira, Sordaini Maria Caligiorne, Rosemeire Brondi Alves, Simone Odilia Antunes Fernandes, Valbert Nascimento Cardoso, Gisele Assis Castro Goulart, Felipe Terra Martins, Maila de Castro Lourenco das Neves, Frederico Duarte Garcia, Angelo de Fatima
Summary: The study introduces two novel calix[n]arene-based anti-cocaine immunogens (V4N2 and V8N2) and evaluates their preclinical efficacy. The immunogens induce high titers of cocaine antibodies and modify the biodistribution of a cocaine analogue, reducing cocaine-induced behavior. These findings demonstrate the potential of V4N2 and V8N2 as immunogens for the treatment of cocaine use disorder.
JOURNAL OF ADVANCED RESEARCH
(2022)
Review
Multidisciplinary Sciences
Yuri de Freitas Rego, Nathalia Evelyn Morais Costa, Rodrigo Martins de Lacerda, Angelica Faleiros da Silva Maia, Cleiton Moreira da Silva, Angelo de Fatima
Summary: Cancer is a group of pathologies caused by cells dividing and multiplying uncontrollably, with chemotherapy being a main treatment approach. Arylchromenes and arylchromans, small molecules of natural or synthetic origin, are of great interest as potential therapeutic prototypes against cancer.
PHYSICAL SCIENCES REVIEWS
(2022)
Article
Agricultural Engineering
Maria Celia Tavares, Carolina Raquel Said Dau Goncalves Olimpio, Angelo de Fatima, Luzia Modolo, Jose Augusto Carneiro de Oliveira, Carlos Jose Alves da Silva-Filho, Francisco Geraldo Barbosa, Jair Mafezoli, Josue Carinhanha Caldas Santos
Summary: Urease catalyzes urea hydrolysis in the soil, leading to N-urea losses by volatilization. This study evaluated the mechanism of urease inhibition by MFC, a compound isolated from Moringa oleifera L. The results showed that MFC is a mixed inhibitor and interacts with urease, causing conformational changes in the protein structure. The interaction also leads to the formation of dithiocarbamates and thiourea, which are inhibitors of urease. MFC shows potential as a biotechnological application compound.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Mycology
Cristina de Castro Spadari, Thayna Lopes Barreto, Vagner Tebaldi de Queiroz, Walysson Ferreira de Paiva, Sergio Antonio Fernandes, Angelo de Fatima, Kelly Ishida
Summary: Water-soluble calix[n]arenes can be used as carrier systems for KTZ to improve its biopharmaceutical properties. CX6Na/KTZ showed superior inhibitory activity against azole-resistant Candida compared to free KTZ, and had no toxic effects on Galleria mellonella larvae.
JOURNAL DE MYCOLOGIE MEDICALE
(2022)
Review
Chemistry, Organic
Walysson Ferreira de Paiva, Yuri de Freitas Rego, Angelo de Fatima, Sergio Antonio Fernandes
Summary: The multicomponent Povarov reaction is a powerful method for synthesizing substances with N-heterocyclic frameworks, capable of forming multiple new bonds.
SYNTHESIS-STUTTGART
(2022)
Review
Chemistry, Organic
Juliana Baptista Simoes, Daniel Leite da Silva, Sergio Antonio Fernandes, Angelo de Fatima
Summary: Calix[n]arenes are considered as versatile organocatalysts due to their adjustable three-dimensional molecular structure, easy synthesis, and compatibility with various reaction systems.
EUROPEAN JOURNAL OF ORGANIC CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Priscila Goes Camargo, Marcieli Fabris, Matheus Yoshimitsu Tatsuta Nakamae, Breno Germano de Freitas Oliveira, Camilo Henrique da Silva Lima, Angelo de Fatima, Marcelle de Lima Ferreira Bispo, Fernando Macedo
Summary: This study investigated the inhibitory effect of hydantoins and thiohydantoins derived from L and n-amino acids on Canavalia ensiformis urease (CEU). The results showed that most thiohydantoins exhibited promising inhibitory potential, with six compounds showing higher inhibitory activity (%I) than the reference inhibitor thiourea (56.5%). Among them, thiohydantoin derived from L-valine (1b) and hydantoin derived from L-methionine (2d) were identified as the most potent inhibitors. The kinetic studies revealed that these compounds exhibited mixed and uncompetitive inhibition, with defined Ki values. Molecular docking studies suggested that the thiohydantoin and hydantoin rings can act as a pharmacophoric group, forming hydrogen bonding interactions with critical amino acid residues in the enzyme active or allosteric site. The stereochemistry of the compounds also influenced their inhibition, kinetics, and binding parameters.
CHEMICO-BIOLOGICAL INTERACTIONS
(2022)
Article
Chemistry, Physical
Werberson de Almeida Bezerra, Jorge Luiz Sonego Milani, Chris Hebert de Jesus Franco, Felipe Terra Martins, Angelo de Fatima, Alvaro Farias Arruda da Mata, Rafael Pavao das Chagas
Summary: A series of 1,2-bis(benzimidazolium)ethane dihydrohalide salts were synthesized and characterized for the cycloaddition reaction of CO2 with epoxides. The salts containing 1,2-bis(5-methyl-benzimidazolium)ethane cation showed the best activity, and the study of various parameters confirmed the stability and high selectivity of the catalyst. A catalytic mechanism was proposed based on H-1 NMR spectroscopy experiments. The simple, nontoxic, and low-cost synthesis of these catalysts, along with high conversions at low catalyst loading, makes them promising for the production of cyclic carbonates using carbon dioxide.
MOLECULAR CATALYSIS
(2022)
Article
Pharmacology & Pharmacy
Ivo S. F. Melo, Victor P. Ziviani, Barbara C. M. Barbosa, Felipe F. Rodrigues, Roger R. L. Silva, Leonardo da Silva Neto, Angelo de Fatima, Isabela C. Cesar, Renes R. Machado, Marcio M. Coelho
Summary: In this study, a novel H2S-releasing phthalimide hybrid was synthesized and demonstrated to have activity in models of inflammatory pain. The compound exerted its effects through the release of H2S and activation of ATP-sensitive potassium channels. This finding stimulates further research to evaluate H2S-releasing phthalimide hybrids as candidate analgesic drugs.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2023)
Article
Pharmacology & Pharmacy
Vagner T. de Queiroz, Bianca de O. Botelho, Natalia A. Guedes, Diana C. Cubides-Roman, Francisco de P. Careta, Jair C. C. Freitas, Daniel F. Cipriano, Adilson V. Costa, Angelo de Fatima, Sergio A. Fernandes
Summary: In this study, an inclusion complex (IC6HKTZ) of ketoconazole (KTZ) with p-sulfonic acid calix[6]arene (CX6SO3H) was prepared to improve the solubility and efficacy of KTZ against Leishmania amazonensis and Leishmania infantum promastigotes. The solubility enhancement of KTZ by 120.00 mu M CX6SO3H was about 95 times. IC6HKTZ exhibited lower IC50 values compared to free KTZ for both Leishmania species, indicating improved anti-leishmanial activities.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Immunology
Brian Sabato, Paulo Sergio de Almeida Augusto, Raissa Lima Gonsalves Pereira, Felipe Coutinho Batista Esteves, Sordaini M. Caligiorne, Bruna Rodrigues Dias Assis, Sostenes Apolo Correia Marcelino, Larissa Pires do Espirito Santo, Karine Dias dos Reis, Leonardo Da Silva Neto, Gisele Goulart, Angelo de Fatima, Felipe Pierezan, Ricardo Toshio Fujiwara, Maila Castro, Frederico Garcia
Summary: An effective method for treating cocaine addiction is the use of anti-drug vaccines. These vaccines stimulate the production of antibodies that can bind to cocaine and reduce its passage through the blood-brain barrier, thereby decreasing its activity in the brain. A new vaccine candidate, UFMG-V4N2, was developed by our research group using an innovative carrier based on calixarenes. Safety and immunogenicity of the anti-cocaine vaccine were assessed in non-human primates, and it was found that UFMG-VAC-V4N2 had minimal side effects and did not affect general health or other physiological parameters. This preclinical study provides important evidence for future clinical trials of the anti-cocaine vaccine.
Article
Chemistry, Analytical
Anne Alves Macedo, Luciano C. Arantes, Dilton Martins Pimentel, Tifany de Deus Melo, Larissa Magalhaes de Almeida Melo, Wellington Alves de Barros, Claudia Mancilha Rocha, Angelo de Fatima, Wallans Torres Pio dos Santos
Summary: This study presents an electrochemical screening method for detecting LSD in forensic samples using a multi-wall carbon nanotube screen-printed electrode. The method offers rapid, sensitive, selective, reproducible, and straightforward preliminary identification of LSD in seized samples.
ANALYTICAL METHODS
(2023)
Article
Chemistry, Medicinal
Taniris Cafiero Braga, Marina Magalhaes Silva, Eduarda O. O. Nascimento, Edjan Carlos Dantas da Silva, Yuri de Freitas Rego, Mullicka Mandal, Zaqueu Alves de Souza, Ana Lucia Tasca Gois Ruiz, Joao Ernesto Carvalho, Felipe Terra Martins, Isis Martins Figueiredo, Thiago Mendonca de Aquino, Cleiton Moreira da Silva, Bhagirath Mandal, Goutam Brahmachari, Josue Carinhanha Caldas Santos, Angelo de Fatima
Summary: Twenty chromenes were synthesized with good to excellent yields (70-96%). Compounds 14, 12, 15, 16, 17, 18, and 20 showed the strongest inhibitory effects on human cancer cell lines. The presence of para-position electron-withdrawing substituents on the phenyl ring favored potency and selectivity for cancer cells. Biophysical studies indicated that certain chromene derivatives preferentially interacted with ctDNA via intercalation, which was consistent with the in vitro biological results.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY REPORTS
(2022)
Article
Toxicology
Wellington Alves de Barros, Camila da Silva Nunes, Juliana Alves da Costa Ribeiro Souza, Igor Jose dos Santos Nascimento, Isis Martins Figueiredo, Thiago Mendonca de Aquino, Leonardo Vieira, Davi Farias, Josue Carinhanha Caldas Santos, Angelo de Fatima
Summary: The toxicological effects of 25H-NBOMe and 25H-NBOH on zebrafish embryos and larvae were demonstrated, including both lethal and non-lethal effects. These drugs not only caused high embryo mortality, but also various malformations and adverse effects. In vitro experiments showed that the drugs have an affinity for DNA, leading to conformational changes in the DNA structure.
CURRENT RESEARCH IN TOXICOLOGY
(2021)