Article
Biochemistry & Molecular Biology
Rita Pancsa, Erzsebet Ficho, Daniel Molnar, Eva Viola Suranyi, Tamas Trombitas, Dora Fuzesi, Hanna Loczi, Peter Szijjarto, Rita Hirmondo, Judit E. Szabo, Judit Toth
Summary: Stimulated by the increasing interest in the role of dNTP pools in physiological and malignant processes, dNTPpoolDB is established to provide quantitative data on dNTP pools and promote research in fields such as cancer progression, development, ageing, and viral infections. The database offers advanced search functions and control-treatment correlations for users to quickly recognize and graphically analyze changes in dNTP pools.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Multidisciplinary Sciences
Fumiaki Ito, Ana L. Alvarez-Cabrera, Kyumin Kim, Z. Hong Zhou, Xiaojiang S. Chen
Summary: The study reveals the mechanism by which HIV-1 virus evades the antiviral response of human APOBEC3 (A3) cytidine deaminases. The cryo-EM structures of the viral infectivity factor (Vif) in complex with A3H, CBF-ss, and components of CUL5 ubiquitin ligase shed light on the interplay between HIV and humans.
NATURE COMMUNICATIONS
(2023)
Article
Microbiology
Xuezhang Tian, Yaru Zhou, Shaowei Wang, Ming Gao, Yanlin Xia, Yangyang Li, Yunhong Zhong, Wenhao Xu, Lei Bai, Bishi Fu, Yu Zhou, Hye-Ra Lee, Hongyu Deng, Ke Lan, Pinghui Feng, Junjie Zhang
Summary: In this study, SMCHD1 was identified as a cell-intrinsic restriction factor that controls the replication of KSHV and a wide range of herpesviruses by targeting the origins of viral DNA replication. SMCHD1 deficiency facilitated the replication of a murine herpesvirus in vivo. This study helps us to better understand intrinsic antiviral immunity and could contribute to the development of new therapies for herpesvirus infection and related diseases.
Article
Cell Biology
Jeffrey R. Johnson, David C. Crosby, Judd F. Hultquist, Andrew P. Kurland, Prithy Adhikary, Donna Li, John Marlett, Justine Swann, Ruth Huttenhain, Erik Verschueren, Tasha L. Johnson, Billy W. Newton, Michael Shales, Viviana A. Simon, Pedro Beltrao, Alan D. Frankel, Alexander Marson, Jeffery S. Cox, Oliver Fregoso, John A. T. Young, Nevan J. Krogan
Summary: Viruses remodel host cellular pathways to replicate and evade immune defenses and they do so with limited genomic coding capacity. This study used mass spectrometry-based proteomics to analyze protein abundance and post-translational modifications in response to HIV-1 infection. The results revealed the importance of Aurora kinase activity in HIV-1 infection and identified potential substrates of a degraded phosphatase during infection. Additionally, the study showed that the HIV-1 Vpr protein inhibits histone H1 ubiquitination, leading to DNA repair defects.
Article
Multidisciplinary Sciences
Guangai Xue, Hyun Jae Yu, Cindy Buffone, Szu-Wei Huang, KyeongEun Lee, Shih Lin Goh, Anna T. T. Gres, Mehmet Hakan Guney, Stefan G. G. Sarafianos, Jeremy Luban, Felipe Diaz-Griffero, Vineet N. N. KewalRamani
Summary: The entry of HIV-1 into the nucleus is regulated by the interaction between the viral capsid protein CA and nuclear pore complex proteins Nup35 and POM121. This interaction is also influenced by soluble host factors such as Cyclophilin A. These findings highlight the role of CA as a macromolecular nuclear transport receptor and provide insights into the mechanism of HIV-1 nuclear invasion.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Qi Peng, Yufeng Xie, Lu Kuai, Han Wang, Jianxun Qi, George F. Gao, Yi Shi
Summary: The World Health Organization declared monkeypox a global public health emergency in July 2022 and urgent measures are needed. Using cryo-electron microscopy, the structure of the monkeypox virus polymerase holoenzyme in complex with DNA was determined. It was found that the holoenzyme utilizes a forward sliding clamp processivity mechanism for viral DNA replication, similar to other B-family DNA polymerases. These findings provide insight into the replication mechanism of the monkeypox virus and may aid in the development of anti-poxvirus drugs.
Article
Virology
Isaura Vanessa Gutierrez, Poulomee Sarkar, Cyprian C. Rossetto
Summary: Kaposi's sarcoma-associated herpesvirus (KSHV) is an oncogenic virus that causes serious diseases in humans. During lytic replication, the virus produces new virions through a cascade of gene expression. ORF59, a viral factor expressed during this process, plays a crucial role in DNA synthesis and interacts with histones and cellular methyltransferases. Studies on ORF59 deletions show specific amino acid regions are required for histone interaction and nuclear localization but not for DNA synthesis, demonstrating its essential role during lytic replication.
JOURNAL OF VIROLOGY
(2021)
Article
Biochemistry & Molecular Biology
Sabrina X. Van Ravenstein, Kavi P. Mehta, Tamar Kavlashvili, Jo Ann W. Byl, Runxiang Zhao, Neil Osheroff, David Cortez, James M. Dewar
Summary: Using Xenopus egg extracts, this study demonstrates that the TOP2 poisons etoposide and doxorubicin inhibit DNA replication through different mechanisms. Etoposide induces TOP2-dependent DNA breaks and TOP2-dependent fork stalling, while doxorubicin stalls replication forks independently of TOP2 by intercalating into parental DNA.
Article
Biochemical Research Methods
Xionghao Lin, Asrar Ahmad, Andrey I. Ivanov, Jyothirmai Simhadri, Songping Wang, Namita Kumari, Tatiana Ammosova, Sergei Nekhai
Summary: The HIV-1 transcription inhibitor 1E7-03 reprogrammed the PPAR alpha/RXR alpha and TGF-6 pathways, leading to the inhibition of HIV-1 transcription. The reduced phosphorylation of nucleophosmin and transforming growth factor-beta 2 played important roles in this process. Additionally, phosphorylation of NPM1 was identified as a crucial mechanism for HIV-1 transcription.
MOLECULAR & CELLULAR PROTEOMICS
(2023)
Review
Immunology
Kumaraswami Chintala, Krishnaveni Mohareer, Sharmistha Banerjee
Summary: Host restriction factors have a significant impact on the viral life cycle, particularly through interferon induction, but HIV-1 can counteract these mechanisms through its encoded components.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Plant Sciences
Zhengjing Zhang, Wenjie Zeng, Wenxin Zhang, Jing Li, Dali Kong, Lei Zhang, Rui Wang, Fangnan Peng, Zhe Kong, Yongping Ke, Heng Zhang, Chanhong Kim, Huiming Zhang, Jose Ramon Botella, Jian-Kang Zhu, Daisuke Miki
Summary: The study established a CRISPR/Cas9-mediated technique for heritable gene targeting in Arabidopsis, achieving precise GT using the sequential transformation method and obtaining vector-free GT plants. The research sheds light on the molecular mechanisms of CRISPR/Cas9-mediated GT in Arabidopsis and provides a broadly applicable technology for precise genome manipulation.
Article
Genetics & Heredity
William Mannherz, Suneet Agarwal
Summary: Genome-wide CRISPR screening identified thymidine nucleotide metabolism as a key regulator of human telomere length. Thymidine supplementation promoted telomere elongation in cells from patients with telomere biology disorders. Our findings highlight the critical role of thymidine metabolism in controlling human telomerase and telomere length.
Article
Immunology
Mingyuan Bi, Wen Kang, Yongtao Sun
Summary: In this study, it was found that HIV infection leads to changes in the transcriptional level of various HSPA subtypes, and HSPA14 interacts with HIV transcriptional inhibitor HspBP1. HIV infection inhibits the expression of HSPA14 in Jurkat and CD4+T cells, while overexpression of HSPA14 inhibits HIV replication and knocking down HSPA14 promotes HIV replication. The expression level of HSPA14 is lower in peripheral blood CD4+T cells of untreated acute HIV infection patients with low viral load. HSPA14 is a potential HIV replication inhibitor that may restrict HIV replication by regulating the transcriptional inhibitor HspBP1. Further studies are needed to determine the specific mechanism by which HSPA14 regulates viral replication.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Yaning Li, Yaping Shen, Ziwei Hu, Renhong Yan
Summary: The ongoing global pandemic caused by a variant of the monkeypox virus has raised concerns. The structure and mechanism of the DNA polymerase holoenzyme of the virus remains elusive, but recent research has provided insights into its assembly and functioning. These findings are important for developing targeted antiviral therapies.
Article
Microbiology
Hibet Errahmanec Chergui, Takfarinas Idres, Chloe Chaudesaigues, Diana Noueihed, Jean Gagnon, Yahia Chebloune
Summary: The study demonstrates that primate lentiviruses are unable to replicate in small ruminants cells due to the lack of functional receptors on the cell surface. However, transfection of primate lentivirus DNA into small ruminant cells can produce infectious viruses, which mainly infect human cells rather than small ruminant cells.
Article
Biochemistry & Molecular Biology
Yuqing Feng, Robin P. Love, Anjuman Ara, Tayyba T. Baig, Madison B. Adolph, Linda Chelico
JOURNAL OF BIOLOGICAL CHEMISTRY
(2015)
Article
Virology
Anjuman Ara, Robin P. Love, Tyson B. Follack, Khawaja A. Ahmed, Madison B. Adolph, Linda Chelico
JOURNAL OF VIROLOGY
(2017)
Article
Multidisciplinary Sciences
Gabriel J. Starrett, Elizabeth M. Luengas, Jennifer L. McCann, Diako Ebrahimi, Nuri A. Temiz, Robin P. Love, Yuqing Feng, Madison B. Adolph, Linda Chelico, Emily K. Law, Michael A. Carpenter, Reuben S. Harris
NATURE COMMUNICATIONS
(2016)
Article
Biochemistry & Molecular Biology
Madison B. Adolph, Robin P. Love, Yuqing Feng, Linda Chelico
NUCLEIC ACIDS RESEARCH
(2017)
Article
Biochemistry & Molecular Biology
Robin P. Love, Huixin Xu, Linda Chelico
JOURNAL OF BIOLOGICAL CHEMISTRY
(2012)
Article
Biochemistry & Molecular Biology
Yuqing Feng, Robin P. Love, Linda Chelico
JOURNAL OF BIOLOGICAL CHEMISTRY
(2013)
Article
Microbiology
Anjuman Ara, Robin P. Love, Linda Chelico
Review
Microbiology
Yuqing Feng, Tayyba T. Baig, Robin P. Love, Linda Chelico
FRONTIERS IN MICROBIOLOGY
(2014)
Meeting Abstract
Biophysics
Michael Morse, Yuqing Feng, Robin P. Love, Ioulia Rouzina, Linda Chelico, Mark C. Williams
BIOPHYSICAL JOURNAL
(2018)