Article
Cell & Tissue Engineering
Yong Tan, Xuewen Duan, Bo Wang, Xingguang Liu, Zhenzhen Zhan
Summary: Irreversible loss of cardiomyocytes following cardiac injury leads to adverse cardiac remodeling and dysfunction. The role of B cells in cardiomyocyte proliferation and heart regeneration is not clear. Our study found that B cell depletion in neonatal mice reduced cardiomyocyte proliferation, causing cardiac dysfunction and failure of heart regeneration. B cell depletion also impaired heart regeneration and function in neonatal mice with myocardial infarction. However, B cell depletion in adult mice suppressed inflammation, improved cardiac function, and inhibited myocardial fibrosis after myocardial infarction. Our study highlights the importance of cardiac B cells in heart regeneration and their divergent effects in neonatal and adult mice.
NPJ REGENERATIVE MEDICINE
(2023)
Review
Cell Biology
Toshiyuki Ko, Seitaro Nomura
Summary: This review summarizes recent studies on manipulating cardiomyocyte plasticity using various approaches such as stem cell differentiation, reprogramming, and reactivation of proliferation as potential treatments for heart disease.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Huixing Zhou, Fulei Zhang, Yahan Wu, Hongyu Liu, Ran Duan, Yuanyuan Liu, Yan Wang, Xiaoyu He, Yuemei Zhang, Xiue Ma, Yi Guan, Yi Liu, Dandan Liang, Liping Zhou, Yi-Han Chen
Summary: The study revealed that low-density lipoprotein receptor-related protein 5 (LRP5) plays a crucial role in neonatal heart regeneration by regulating cardiomyocyte proliferation. LRP5 expression gradually decreased after birth in mice, consistent with the withdrawal of cardiomyocytes from the cell cycle. LRP5 downregulation reduced the proliferation of neonatal cardiomyocytes, while LRP5 overexpression promoted cardiomyocyte proliferation. The AKT/P21 signaling pathway was identified as the key pathway regulating cardiomyocyte proliferation mediated by LRP5.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2022)
Article
Cardiac & Cardiovascular Systems
Alexandra L. Purdy, Samantha K. Swift, Henry M. Sucov, Michaela Patterson
Summary: We conducted a retrospective analysis of single cell ventricular suspensions to identify cardiomyocytes that have completed cell division. Our findings indicate that the ability of cardiomyocytes to re-enter the cell cycle and complete cell division after injury is independent and variable. Additionally, we provide evidence that Tnni3k influences both early and final stages of the cell cycle.
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
(2023)
Review
Cardiac & Cardiovascular Systems
John Auchampach, Lu Han, Guo N. Huang, Bernhard Kuhn, John W. Lough, Caitlin C. O'Meara, Alexander Y. Payumo, Nadia A. Rosenthal, Henry M. Sucov, Katherine E. Yutzey, Michaela Patterson
Summary: The field of mammalian myocardial regeneration has seen significant growth in the past two decades. However, there are limitations and challenges in characterizing cardiomyocyte proliferation. Claims of cardiomyocyte proliferation often lack sufficient evidence or proper controls, and the provided data may only indicate cell-cycle activation or DNA synthesis, without proving the generation of new cardiomyocytes. Rigorous experimental strategies and standards should be used to support claims of proliferation-based remuscularization.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2022)
Article
Developmental Biology
Daniel A. Zuppo, Maria A. Missinato, Lucas Santana-Santos, Guang Li, Panayiotis Benos, Michael Tsang
Summary: The poor regenerative capacity of the mammalian heart may be due to the inability of adult cardiomyocytes to proliferate at sufficient levels. However, research has shown that the transcription factor Foxm1 can induce cardiomyocyte proliferation after injury through regulation of cell cycle genes.
Article
Cell Biology
Michka Sharpe, Juan Manuel Gonzalez-Rosa, Felicia Wranitz, Spencer Jeffrey, Katherine Copenhaver, C. Geoffrey Burns, Caroline E. Burns
Summary: Cardiomyocyte proliferation is an important source of new myocardium during heart development and regeneration, and Ruvbl2 functions as a suppressor of cardiomyocyte proliferation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Medicine, Research & Experimental
Zhenbo Han, Xiuxiu Wang, Zihang Xu, Yang Cao, Rui Gong, Yang Yu, Ying Yu, Xiaofei Guo, Shenzhen Liu, Meixi Yu, Wenya Ma, Yiming Zhao, Juan Xu, Xingda Li, Shuainan Li, Yan Xu, Ruijie Song, Binbin Xu, Fan Yang, Djibril Bamba, Natalia Sukhareva, Hong Lei, Manqi Gao, Wenwen Zhang, Naufal Zagidullin, Ying Zhang, Baofeng Yang, Zhenwei Pan, Benzhi Cai
Summary: The study reveals the significant role of m(6)A RNA modification in heart regeneration, with the ALKBH5-m(6)A-YTHDF1-YAP axis regulating cardiomyocytes to re-enter the cell cycle. This finding offers a novel therapeutic strategy for cardiac regeneration.
Article
Biochemistry & Molecular Biology
Wenyuan Wang, Ye-Fan Hu, Meijun Pang, Nannan Chang, Chunxiao Yu, Qi Li, Jing-Wei Xiong, Yuanyuan Peng, Ruilin Zhang
Summary: The study demonstrated the crucial roles of BMP and Notch signaling pathways in zebrafish heart regeneration, but the interaction between them and the specific mechanisms in cardiomyocyte proliferation still require further exploration.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Article
Cardiac & Cardiovascular Systems
Chengzhen Huang, Tong Ding, Yuan Zhang, Xunkai Li, Xin Sun, Shuangjie Lv, Yanyan Hao, Lina Bai, Ning Liu, Yifan Xie, Houzao Chen, Yu Nie
Summary: The protein p66Shc plays a crucial role in heart regeneration by promoting neonatal mouse cardiomyocyte proliferation and facilitating heart regeneration. The study also reveals that p66Shc regulates cardiomyocyte proliferation through the activation of beta-catenin signaling. These findings provide new insights into senescence signaling in heart regeneration.
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
(2023)
Article
Cell Biology
Shijie Liu, Ke Li, Leonardo Wagner Florencio, Li Tang, Todd R. Heallen, John P. Leach, Yidan Wang, Francisco Grisanti, James T. Willerson, Emerson C. Perin, Sui Zhang, James F. Martin
Summary: The study demonstrates the potential therapeutic value of local knockdown of the Hippo pathway gene Salvador in pig hearts using AAV9 gene therapy, leading to tissue renewal and improved function in heart failure.
SCIENCE TRANSLATIONAL MEDICINE
(2021)
Article
Multidisciplinary Sciences
Phong D. Nguyen, Iris Gooijers, Giulia Campostrini, Arie O. Verkerk, Hessel Honkoop, Mara Bouwman, Dennis E. M. de Bakker, Tim Koopmans, Aryan Vink, Gerda E. M. Lamers, Avraham Shakked, Jonas Mars, Aat A. Mulder, Sonja Chocron, Kerstin Bartscherer, Eldad Tzahor, Christine L. Mummery, Teun P. de Boer, Milena Bellin, Jeroen Bakkers
Summary: Zebrafish hearts can regenerate by replacing damaged tissue with new cardiomyocytes. The cardiac dyad, a structure that regulates calcium handling and excitation-contraction coupling, plays a key role in the redifferentiation process.
Article
Cardiac & Cardiovascular Systems
Fabien Hubert, Sandy M. Payan, Edeline Pelce, Laetitia Bouchard, Rachel Sturny, Nicolas Lenfant, Giovanna Mottola, Frederic Collart, Robert G. Kelly, Francesca Rochais
Summary: This study demonstrates that FGF10 promotes cardiac regeneration and repair through elevating cardiomyocyte renewal and limiting fibrosis, suggesting its potential as a clinically relevant target for heart regeneration and repair.
CARDIOVASCULAR RESEARCH
(2022)
Review
Biochemistry & Molecular Biology
Abou Bakr M. Salama, Ahmad Gebreil, Tamer M. A. Mohamed, Riham R. E. Abouleisa
Summary: This article discusses the inability of the human heart to regenerate fully compared to some lower vertebrates, and explores the latest research progress on inducing cardiomyocyte cell cycle entry and heart repair through altering cardiomyocyte plasticity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Andrea Raso, Ellen Dirkx, Vasco Sampaio-Pinto, Hamid el Azzouzi, Ryan J. Cubero, Daniel W. Sorensen, Lara Ottaviani, Serve Olieslagers, Manon M. Huibers, Roel de Weger, Sailay Siddiqi, Silvia Moimas, Consuelo Torrini, Lorena Zentillin, Luca Braga, Diana S. Nascimento, Paula A. da Costa Martins, Jop H. van Berlo, Serena Zacchigna, Mauro Giacca, Leon J. De Windt
Summary: miR-106b similar to 25 cluster is highly expressed in early postnatal myocardium and plays a crucial role in transitioning cardiomyocyte hyperplasia to cell cycle arrest and hypertrophy. This cluster can enhance the regenerative capacity of the injured heart by targeting a network of negative cell cycle regulators.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Mirjam Brackhan, Giulio Calza, Kristiina Lundgren, Pablo Bascunana, Thomas Bruning, Rabah Soliymani, Rakesh Kumar, Axel Abelein, Marc Baumann, Maciej Lalowski, Jens Pahnke
Summary: Using stable isotope labeling and targeted mass spectrometry, the study shows that amyloid-beta does not reach the brain from the periphery, contradicting the theory of transmissibility in Alzheimer's disease.
Article
Biochemistry & Molecular Biology
Jussi Leinonen, Paivi Leinikka, Miikka Tarkia, Milla Lampinen, Avishag K. Emanuelov, Ronen Beeri, Esko Kankuri, Eero Mervaala
Summary: The left atrial appendage (LAA) of the adult heart contains progenitor cells and can be used as a regenerative transplant for myocardial infarction. Transplantation of LAA to mice with MI resulted in improved cardiac function and inhibition of deleterious remodeling. This method combines cell therapy and physical support.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Cell Biology
Annunziata Cascone, Maciej Lalowski, Dan Lindholm, Ove Eriksson
Summary: LACTB is a relatively unknown mitochondrial protein that may play important roles in lipid metabolism and tumorigenesis. Despite numerous studies, the precise biochemical and cell biological function of LACTB remains unclear. The recently published cryo-electron microscopy structure of LACTB's filamentous form provides valuable insights for further research. Resolving these questions will enhance our understanding of the physiological functions of LACTB in cells and organisms.
Article
Cell Biology
Stefano Doccini, Maria Marchese, Federica Morani, Nicola Gammaldi, Serena Mero, Francesco Pezzini, Rabah Soliymani, Melissa Santi, Giovanni Signore, Asahi Ogi, Silvia Rocchiccioli, Katja M. Kanninen, Alessandro Simonati, Maciej M. Lalowski, Filippo M. Santorelli
Summary: This study reveals the key role of lysosomes and lipid metabolism in CLN5 disease through proteomics analysis and functional validation. It also identifies NCL biomarkers with high lysosomal confidence. Furthermore, the findings are validated in a zebrafish model and the therapeutic effects of two FDA-approved drugs are evaluated.
Article
Pharmacology & Pharmacy
Venla Kurra, Arttu Eraranta, Timo Paavonen, Teemu Honkanen, Juhani Myllymaki, Asko Riutta, Ilkka Tikkanen, Paivi Lakkisto, Jukka Mustonen, Ilkka Porsti
Summary: Experimental hyperuricaemia had positive effects on the kidney of surgically 5/6 nephrectomized rats, showing improved kidney morphology and reduced markers of oxidative stress and inflammation.
BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY
(2023)
Review
Medical Laboratory Technology
Paivi Lakkisto, Louise Torp Dalgaard, Thalia Belmonte, Sara-Joan Pinto-Sietsma, Yvan Devaux, David De Gonzalo-Calvo
Summary: The implementation of cell-free microRNAs as biomarkers is limited due to lack of reproducibility. Technical variability and analytical caveats make the interpretation of miRNA data challenging. Standardization efforts and consideration of specific aspects in sample handling and data analysis are important for reproducibility. Providing a checklist can facilitate the control of miRNA quantification and analysis.
CRITICAL REVIEWS IN CLINICAL LABORATORY SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Kristina Maemets-Allas, Mariliis Klaas, Claudia Griselda Cardenas-Leon, Terje Arak, Esko Kankuri, Viljar Jaks
Summary: Similar to other mammalian tissues, the extracellular matrix (ECM) in the skin serves as a supportive and regulatory environment for cell behavior. It is primarily composed of structural and nonstructural proteins, with the latter, including thrombospondin-4 (THBS4), being dynamically regulated. Previous research showed that THBS4 promotes cutaneous wound healing, and this study reveals its additional role in stimulating the migration of primary keratinocytes in a 3D environment. Through proteotranscriptomic analysis, it was found that THBS4 regulates signaling pathways associated with proliferation, migration, inflammation, and differentiation in keratinocytes, some of which are implicated in the development of atopic dermatitis (AD). Overall, THBS4 shows promise as a candidate for novel wound healing therapies, and there may be overlap between the pathways involved in cutaneous wound healing and those active in inflammatory skin diseases.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Nanoscience & Nanotechnology
Ya Zhu, Esko Kankuri, Xue Zhang, Zhangmin Wan, Xin Wang, Siqi Huan, Long Bai, Shouxin Liu, Orlando J. Rojas
Summary: Direct ink writing (DIW) is used to engineer complex constructs from biobased colloids, with the challenges of strong water interactions and lack of interparticle connectivity overcome by using low-solid emulgel inks stabilized by chitin nanofibrils. Through complementary characterization platforms and molecular dynamics simulations, the structured three-dimensional materials with multiscale porosities are obtained, leading to excellent modulation of cell behavior on the scaffolds.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Obstetrics & Gynecology
Tea Kuittinen, Sari Tulokas, Paivi Rahkola-Soisalo, Tea Brummer, Jyrki Jalkanen, Eija Tomas, Juha Makinen, Jari Sjoberg, Paivi Harkki, Maarit Mentula
Summary: Hysterectomy may have a small impact on the risk of pelvic organ prolapse surgeries and visits among women without a preexisting prolapse. Laparoscopic-assisted vaginal hysterectomy, vaginal deliveries, and concomitant stress urinary incontinence operations were associated with increased risk of prolapse surgeries after hysterectomy. These findings can be useful for counseling women considering hysterectomy for benign indications.
ACTA OBSTETRICIA ET GYNECOLOGICA SCANDINAVICA
(2023)
Article
Behavioral Sciences
Silvia Poggini, Maria Banqueri Lopez, Naomi Ciano Albanese, Maria Teresa Golia, Fernando Gonzalez Ibanez, Cristina Limatola, Martin Furhmann, Maciej Lalowski, Marie -Eve Tremblay, Laura Maggi, Bozena Kaminska, Igor Branchi
Summary: Major depressive disorder (MDD) is a chronic and potentially life-threatening illness that affects millions of people worldwide. The currently available treatments for cognitive deficits in MDD are limited. However, minocycline, an antibiotic with anti-inflammatory properties, has shown potential as a treatment for cognitive impairment. This study explored the effects of minocycline on cognition in a mouse model of depression, and found that minocycline improved cognitive performance and hippocampal plasticity in the short term, but had no lasting effects on microglia.
BEHAVIOURAL BRAIN RESEARCH
(2023)
Editorial Material
Medicine, Research & Experimental
Esko Kankuri
MOLECULAR THERAPY-NUCLEIC ACIDS
(2023)
Article
Immunology
Fernando Gonzalez Ibanez, Torin Halvorson, Kaushik Sharma, Chloe Grace Mckeec, Micael Carrier, Katherine Picard, Nathalie Vernoux, Kanchan Bisht, Jessica Deslauriers, Maciej Lalowski, Marie-Eve Tremblay
Summary: Psychological stress increases the risk of various diseases, while the ketogenic diet (KD) has positive effects on mood and health. This study reveals that KD may improve resistance to psychological stress by its anti-inflammatory effects on the brain and behavior, as well as its impact on microglial morphology and function.
BRAIN BEHAVIOR AND IMMUNITY
(2023)
Article
Biochemistry & Molecular Biology
E. Kankuri, P. Finckenberg, J. Leinonen, M. Tarkia, S. Bjork, J. Purhonen, J. Kallijaervi, M. Kankainen, R. Soliymani, M. Lalowski, E. Mervaala
Summary: The regenerative capacity of myocardium declines in the first week after birth, and this decline is related to adaptation to oxidative metabolism. By studying mice, researchers found that promoting mitochondrial fatty acid transport and improving the beta-oxidation pathway can overcome metabolic barriers for repair and regeneration after myocardial infarction and heart failure in adult mammals.
EXPERIMENTAL AND MOLECULAR MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Hanna Lahteenmaki, Tommi Patila, Ismo T. Raisanen, Esko Kankuri, Taina Tervahartiala, Timo Sorsa
Summary: This pilot study suggests that regular dual-light aPDT treatment at home can be beneficial for peri-implantitis. Frequent repeated applications can reduce microbial burden and lower tissue destructive proteolytic and inflammatory load. Further large-scale studies are needed to demonstrate long-term benefits.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2022)