Review
Microbiology
Nazanin Jafari, Arezoo Khoradmehr, Reza Moghiminasr, Mina Seyed Habashi
Summary: The oral microbiome is a diverse microbial community in the oral cavity, including bacteria, fungi, viruses, and protozoa. Immunity plays a crucial role in defending against orodental infectious diseases. Exosomes, lipid bilayer vesicles secreted by immune cells, can modulate immune and inflammatory responses, potentially serving as a therapeutic strategy for orodental infectious diseases.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Immunology
Peishan Li, Qianmin Ou, Songtao Shi, Changshun Shao
Summary: Mesenchymal stem/stromal cells (MSCs) are widely distributed in the body and can be used for the treatment of autoimmune diseases and other chronic disorders. Dental stem cells (DSCs) have remarkable immunomodulatory properties and have therapeutic effects on systemic inflammatory diseases. MSCs derived from nondental tissues, such as the umbilical cord, also show benefits in the management of periodontitis in preclinical studies.
CELLULAR & MOLECULAR IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Veronika Smolinska, Michaela Debreova, Martina Culenova, Maria Csobonyeiova, Andrey Svec, Lubos Danisovic
Summary: Healing of articular cartilage defects is challenging and traditional reparative approaches are limited. Mesenchymal stromal cells have been extensively studied and proven to have therapeutic effects on articular cartilage.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Medicine, Research & Experimental
Mohammad Rudiansyah, Amr A. El-Sehrawy, Irfan Ahmad, Ermias Mergia Terefe, Walid Kamal Abdelbasset, Dmitry Olegovich Bokov, Aleli Salazar, Jasur Alimdjanovich Rizaev, Fares Mohammed Saeed Muthanna, Mohammed Nader Shalaby
Summary: Osteoporosis is a common and serious bone problem, especially in the elderly. Current treatments focus on pain relief but not on bone tissue recovery. However, stem cell therapy and exosomes are considered effective methods for treating and improving osteoporosis.
Review
Cell Biology
Maria I. Quinones-Vico, Raquel Sanabria de la Torre, Manuel Sanchez-Diaz, Alvaro Sierra-Sanchez, Trinidad Montero-Vilchez, Ana Fernandez-Gonzalez, Salvador Arias-Santiago
Summary: The skin, the largest organ of the human body, has important functions including protection and maintenance. MSCs and their secretome, particularly exosomes, are believed to play a significant role in skin repair and regeneration, indicating the need for further research for clinical translation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Dentistry, Oral Surgery & Medicine
L. Yao, F. Li, C. Yu, H. Wang, Y. Wang, L. Ye, F. Yu
Summary: The influence of chronological and replicative senescence on CD51(+)/PDGFR-alpha(+) hDPSCs was investigated using various experimental methods. Chronological senescence led to decreased CD51(+)/PDGFR-alpha(+) hDPSCs, impaired self-renewal, and higher ossificatory differentiation. Replicative senescence resulted in decreased CD51 expression, upregulated PDGFR-alpha expression, and weakened self-renewal and osteogenic differentiation. These findings highlight the distinct outcomes and core dysfunction of chronological and replicative senescence in CD51(+)/PDGFR-alpha(+) hDPSCs.
JOURNAL OF DENTAL RESEARCH
(2023)
Review
Biochemistry & Molecular Biology
Angelos Matheakakis, Aristea Batsali, Helen A. Papadaki, Charalampos G. Pontikoglou
Summary: Mesenchymal stromal cells (MSCs) are multipotent stem cells with potential applications in tissue repair and treatment of autoimmune disorders. MSC-EVs play a key role in intercellular communication by carrying various signaling factors, exhibiting immunomodulatory potential, and emerging as an appealing alternative therapeutic option.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Chenghai Li, Hua Zhao, Linna Cheng, Bin Wang
Summary: MSC-based therapeutics are available for a range of diseases, with autologous and allogeneic MSCs having their own advantages and disadvantages in medical practice. The interaction of transplanted MSCs with their microenvironment can be impacted by factors from different donors, potentially leading to uncertainty in safety and efficacy. Donor-controlled clinical practice is suggested to minimize potential harm in MSC-based therapies.
CELL AND BIOSCIENCE
(2021)
Article
Immunology
Pauline Rozier, Marie Maumus, Alexandre Thibault Jacques Maria, Karine Toupet, Josephine Lai-Kee-Him, Christian Jorgensen, Philippe Guilpain, Daniele Noel
Summary: This study demonstrates that EVs derived from MSCs and ASCs have a therapeutic effect on systemic sclerosis by slowing down the disease progression. Down-regulating miR-29a-3p in MSCs abolishes the therapeutic efficacy, highlighting the importance of this microRNA in the treatment. Furthermore, EVs from A29a-transfected ASCs failed to improve skin fibrosis, indicating the potential role of miR-29a-3p in regulating methylation and apoptosis pathways in SSc.
JOURNAL OF AUTOIMMUNITY
(2021)
Article
Biochemistry & Molecular Biology
Marta Clos-Sansalvador, Sergio G. Garcia, Miriam Moron-Font, Charles Williams, Niels-Christian Reichardt, Juan M. Falcon-Perez, Antoni Bayes-Genis, Santiago Roura, Marcella Franquesa, Marta Monguio-Tortajada, Francesc E. Borras
Summary: This study reveals the importance of N-glycosylation in MSC-EV for their interaction with endothelial cells and associated functions, such as cell uptake, migration, and angiogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Pharmacology & Pharmacy
Giulia Brunello, Federica Zanotti, Martina Trentini, Ilaria Zanolla, Elham Pishavar, Vittorio Favero, Riccardo Favero, Lorenzo Favero, Eriberto Bressan, Massimo Bonora, Stefano Sivolella, Barbara Zavan
Summary: This study found that EXOs derived from dental pulp stem cells of different age donors have varying effects on MSCs. The younger donor group showed a more significant influence on proliferation and commitment ability.
Review
Cell & Tissue Engineering
Xueqing Zhu, Dan Ma, Baoqi Yang, Qi An, Jingwen Zhao, Xinnan Gao, Liyun Zhang
Summary: Autoimmune/inflammatory diseases are a major global health issue. Mesenchymal stem cells (MSCs) have unique biological characteristics and can be used in the treatment of these diseases due to their immunoregulatory and tissue repairing functions. In addition, MSCs can secrete extracellular vesicles, particularly exosomes, which can be engineered to improve their homing and therapeutic effects. This review article summarizes the advanced technology in engineering MSCs or MSC-derived exosomes and their applications in autoimmune/inflammatory diseases based on recent literature.
STEM CELL RESEARCH & THERAPY
(2023)
Review
Cell & Tissue Engineering
Yuxiang Zhang, Wei Ju, Hong Zhang, Liu Mengyun, Weiliang Shen, Xiao Chen
Summary: Tendinopathy is a disease caused by the degeneration of tendon tissue, and currently, there is no treatment available to prevent or delay its progression. Recent research suggests that MSCs-derived exosomes (MSCs-exos) may be useful for promoting tendon healing and could be considered as a potential treatment for tendinopathy.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Cell Biology
Beverly Brooks, Dominique Ebedes, Ahsan Usmani, Joaquin Vega Gonzales-Portillo, Daniel Gonzales-Portillo, Cesario Borlongan
Summary: Ischemic brain injury is a major cause of death worldwide with limited treatment options. Stem cell-based regenerative therapies, specifically mesenchymal stromal cells, show promise in stroke treatment, but further research is needed to fully understand their cell function.
Article
Biochemistry & Molecular Biology
Agnieszka Krawczenko, Aleksandra Klimczak
Summary: MSCs, known for their regenerative and immunomodulatory properties, are typically isolated from bone marrow and adipose tissue. This review focuses on MSCs derived from adipose tissue and their secretome in regeneration processes, with a special emphasis on their contribution to angiogenic processes. Various clinical trials have been registered to explore the application of AT-MSCs and their secretome in treating different medical conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Ursula Altanerova, Jana Jakubechova, Katarina Benejova, Petra Priscakova, Martin Pesta, Pavel Pitule, Ondrej Topolcan, Juraj Kausitz, Martina Zduriencikova, Vanda Repiska, Cestmir Altaner
INTERNATIONAL JOURNAL OF CANCER
(2019)
Article
Oncology
Andrea Pastorakova, Jana Jakubechova, Ursula Altanerova, Cestmir Altaner
Article
Oncology
Ursula Altanerova, Jana Jakubechova, Katarina Benejova, Petra Priscakova, Vanda Repiska, Andrea Babelova, Bozena Smolkova, Cestmir Altaner
Summary: Exosomes containing suicide gene mRNA secreted from engineered MSCs have the potential to be a specific therapy for tumors and potentially for metastases by inducing cell death in tumor cells without inhibiting the growth of normal cells. This innovative approach utilizes exosomes as a delivery system for suicide genes, showing promising results in cancer treatment.
INTERNATIONAL JOURNAL OF CANCER
(2021)
Article
Genetics & Heredity
Katarina Fabisikova, Olivia Hamidova, Regina Lohajova Behulova, Katarina Zavodna, Petra Priscakova, Vanda Repiska
FRONTIERS IN GENETICS
(2020)
Article
Biochemistry & Molecular Biology
Dominika Sersenova, Zdenko Machala, Vanda Repiska, Helena Gbelcova
Summary: Plasma medicine is a new field focusing on biomedical and clinical applications of cold gas plasmas, including their anticancer effects. Effects of plasma-activated liquids (PAL) on cancer cells showed selectivity compared to normal fibroblasts, depending on correctly chosen activation time and PAL concentration. Plasma-generated hydrogen peroxide may play a role in the selectivity effect of PAL on cancer cells.
Article
Biochemistry & Molecular Biology
Jan Viglas, Simona Dobiasova, Jitka Viktorova, Tomas Ruml, Vanda Repiska, Petra Olejnikova, Helena Gbelcova
Summary: This study investigated the production of peptaibols by Trichoderma atroviride O1 and their antibacterial and anticancer activity against drug-sensitive and multi-drug resistant bacteria and cancer cell lines. The results showed that peptaibols exhibited significant inhibitory effects on the growth of methicillin-resistant Staphylococcus aureus and human breast cancer and ovarian cancer cell lines.
Article
Oncology
Miroslav Tibensky, Jana Jakubechova, Ursula Altanerova, Andrea Pastorakova, Boris Rychly, Ladislav Baciak, Boris Mravec, Cestmir Altaner
Summary: Extracellular vesicles secreted by human mesenchymal stem/stromal cells can be used to treat high-grade glioblastomas. These therapeutic vesicles, engineered with a gene, can inhibit the growth of glioblastoma cells in the presence of a specific drug. Animal experiments have shown the potential of this new cell-free therapy for tumor treatment.
Article
Obstetrics & Gynecology
L. Gergely, R. Petrovic, Z. Feketova, D. Klimova, L. Danihel, K. Mosna, H. Mrazova, M. Korbe, V Repiska, P. Priscakova
Summary: Genetic analysis can assist in accurate diagnosis of partial hydatidiform mole, leading to appropriate medical care for patients.
CESKA GYNEKOLOGIE-CZECH GYNAECOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Gergely Buglyo, Jakub Styk, Ondrej Pos, Adam Csok, Vanda Repiska, Beata Soltesz, Tomas Szemes, Balint Nagy
Summary: Lynch syndrome is an inherited cancer predisposition disorder that can lead to various types of cancers. Current diagnosis methods involve testing for MMR protein deficiency, mutation detection, microsatellite instability, and MLH1 promoter hypermethylation. Recent studies have shown the potential of using DNA and RNA markers extracted from liquid biopsies for diagnosing LS.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Oncology
Jakub Styk, Gergely Buglyo, Ondrej Pos, Adam Csok, Beata Soltesz, Peter Lukasz, Vanda Repiska, Balint Nagy, Tomas Szemes
Summary: This article provides an overview of the application of liquid biopsy in the diagnosis and management of colorectal cancer (CRC). It discusses the use of liquid biopsy-derived DNA and RNA biomarkers for early detection and monitoring of disease recurrence. The article also explores the genetic causes of CRC, the advantages and difficulties of liquid biopsy, and the potential for personalized management.
Article
Biochemical Research Methods
Daniela Klimova, Jana Jakubechova, Ursula Altanerova, Andreas Nicodemou, Jakub Styk, Tomas Szemes, Vanda Repiska, Cestmir Altaner
Summary: Extracellular vesicles (EVs) are utilized as a drug delivery tool in cancer therapy. We engineered mesenchymal stem/stromal cells (MSCs) to secrete EVs packed with chemotherapeutic agents, which were then delivered directly into tumor cells. The EVs inhibited the growth of pancreatic carcinoma cell lines in vitro, and the MSCs were also engineered to express a suicide gene that converted a non-toxic prodrug into a highly cytotoxic drug in cancer cells, further inhibiting their growth.
MOLECULAR AND CELLULAR PROBES
(2023)
Article
Medicine, General & Internal
V Repiska, J. Styk, H. Gbelcova, P. Priscakova, S. Humplikov, M. Bernadic, D. Klimova
Summary: This study compared the statistical calculations of DNA tweezers' reaction time using three different methods and found that different methods produced different results.
BRATISLAVA MEDICAL JOURNAL-BRATISLAVSKE LEKARSKE LISTY
(2021)
Article
Obstetrics & Gynecology
L. Gergely, H. Gbelcova, V Repiska, L. Danihel, M. Korbel, P. Priscakova
CESKA GYNEKOLOGIE-CZECH GYNAECOLOGY
(2020)
Article
Medicine, General & Internal
M. Skorvanova, T. Matakova, M. Skerenova, M. Sarlinova, H. Drobkova, M. Petras, M. Janickova, M. Halasa, V Repiska, E. Halasova
BRATISLAVA MEDICAL JOURNAL-BRATISLAVSKE LEKARSKE LISTY
(2020)
Review
Endocrinology & Metabolism
Katarina Fabisikova, Regina Lohajova Behulova, Vanda Repiska
NEUROENDOCRINOLOGY LETTERS
(2019)