Article
Biochemistry & Molecular Biology
Danyu Yao, Waqas Ijaz, Yi Liu, Jinghuang Hu, Wentao Peng, Bowen Zhang, Xiaolan Wen, Juan Wang, Dan Qiu, Hongjie Li, Shihe Xiao, Guozhong Sun
Summary: In this study, the powdery mildew resistance gene pmXMM was localized in Chinese landrace Xiaomaomai using bulked segregant RNA sequencing and comparative genomics analysis. Genetic analysis and SNP markers revealed that pmXMM is located at the distal end of chromosome 2AL. It was confirmed that Xiaomaomai carries a Pm4 allele and pmXMM is identical to Pm4d and Pm4e at the sequence level.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Meng Sun, Qi Liu, Yi Han, Guojun Liu, Jiajie Wu, Juan Qi, Fei Ni, Yinguang Bao
Summary: Powdery mildew, caused by Blumeria graminis f. sp. tritici, is a devastating fungal disease affecting wheat worldwide. Identifying new resistance genes and breeding resistant varieties are effective strategies to combat this disease. Genetic and molecular analysis of the resistant breeding line SN15218 led to the discovery of the new resistance gene Pm-SN15218 located on chromosome 2AL.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Pengtao Ma, Liru Wu, Yufei Xu, Hongxing Xu, Xu Zhang, Wenrui Wang, Cheng Liu, Bo Wang
Summary: Through genetic analysis and RNA-Seq technology, this study identified a dominant gene PmYD588 related to powdery mildew resistance in a wheat genotype, located on chromosome 2B. By using genomic approaches, candidate regions of this gene were determined and potential regulatory genes were revealed. These findings contribute to the understanding of wheat resistance mechanisms against powdery mildew and the improvement of disease resistance.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Agronomy
Liru Wu, Tong Zhu, Huagang He, Xinyou Cao, Haosheng Li, Hongxing Xu, Mengshu Jia, Lipei Zhang, Jiancheng Song, Ghader Mirzaghaderi, Cheng Liu, Pengtao Ma
Summary: This study identified a new resistance gene PmLS5082 against powdery mildew in wheat through genetic analysis and RNA-Seq technology. By mapping and validating associated genes, important information for marker-assisted selection was provided.
Article
Plant Sciences
Xiao Liang, Hongxing Xu, Shanying Zhu, Yongshen Zheng, Wen Zhong, Haosheng Li, Liping Niu, Liru Wu, Lipei Zhang, Jiancheng Song, Huagang He, Cheng Liu, Pengtao Ma
Summary: In this study, a new dominant gene PmPBDH was identified as the source of high resistance to powdery mildew in wheat breeding line PBDH1607. Genetic analysis and mapping revealed that PmPBDH is located on chromosome arm 4AL within a specific interval. Candidate genes associated with PmPBDH were also identified, and linked markers were developed for marker-assisted breeding.
Article
Plant Sciences
Xiaohan Shi, Peipei Wu, Jinghuang Hu, Dan Qiu, Yunfeng Qu, Yahui Li, Yi Liu, Tesfay Gebrekirstos Gebremariam, Jingzhong Xie, Qiuhong Wu, Hongjun Zhang, Hongwei Liu, Li Yang, Guozhong Sun, Yang Zhou, Zhiyong Liu, Hongjie Li
Summary: The study identified the resistant genes Pm52 and Qaprpm.caas.2B, Qaprpm.caas.7A in winter wheat cultivar Liangxing 99 against powdery mildew through genetic analysis and gene mapping, providing important clues for further breeding efforts.
Article
Agronomy
Zhaohui Zhang, Kuanhong Wang, Chunhong Chen, Shoubo Tian, Jue Wu, Jianyong Li, Lingjuan Kong, Xiaofeng Yang, Changwei Zhang, Ying Li, Hongfang Zhu, Dong Xiao
Summary: RNA sequencing technology was used to investigate the changes in leaf transcriptome profiles of bottle gourd after inoculation with powdery mildew. Several metabolic pathways related to disease resistance were upregulated in the resistant plants, including phenylpropanoid biosynthesis, starch and sucrose metabolism, and plant-pathogen interaction. Enhancing these pathways can improve the resistance and tolerance of bottle gourd to powdery mildew, providing a basis for further breeding for resistance.
Article
Plant Sciences
Jinghuang Hu, Tesfay Gebrekirstos Gebremariam, Peng Zhang, Yunfeng Qu, Dan Qiu, Xiaohan Shi, Yahui Li, Qiuhong Wu, Ming Luo, Lijian Yang, Hongjun Zhang, Li Yang, Hongwei Liu, Yang Zhou, Zhiyong Liu, Baotong Wang, Hongjie Li
Summary: Winter wheat line Tianmin 668 was crossed with susceptible cultivar Jingshuang 16 to develop 216 recombinant inbred lines (RILs) for analyzing its adult-plant resistance (APR) and all-stage resistance (ASR) against powdery mildew. Three loci-QPmtj.caas-2BL, QPmtj.caas-2AS, and QPmtj.caas-5AL-conferring APR to powdery mildew were detected on chromosomes 2BL, 2AS, and 5AL, respectively, of Tianmin 668. A Kompetitive allele-specific PCR marker specific for QPmtj.caas-2BL was developed and verified on 402 wheat cultivars or breeding lines.
Article
Agriculture, Dairy & Animal Science
Ju Qiu, Rui Guo, Yidan Li, Yuyao Zhang, Kangsheng Jia, Yinghu Lei, Linsen Zan, Anning Li
Summary: The golden takin, an endemic ruminant species, has been listed as endangered and vulnerable. This study used RNA sequencing and microsatellite prediction to assess the transcriptome of its muscles, providing valuable genetic information for conservation efforts and further studies on takins.
Article
Horticulture
Yanyan Cao, Qiannan Diao, Shijun Lu, Yongping Zhang, Dongwei Yao
Summary: This study conducted RNA-seq analysis of resistant and susceptible melons to powdery mildew (PM) infection and identified significant differentially expressed genes (DEGs) related to PM resistance. The study revealed the different response and regulatory network between resistant and susceptible melons to PM infection. The study also found that genes related to phenylpropanoid biosynthesis and encoding glucan endo-1,3-beta- glucosidases and cytochrome P450 proteins may play important roles in the resistance to P. xanthii infection.
SCIENTIA HORTICULTURAE
(2022)
Article
Agronomy
Qiuhong Wu, Yongxing Chen, Jingzhong Xie, Lingli Dong, Zhenzhong Wang, Ping Lu, Rongge Wang, Chengguo Yuan, Yan Zhang, Zhiyong Liu
Summary: Reduced plant height is an important trait related to lodging resistance and crop yield, and the use of reduced height genes in breeding can lead to modern high-yielding wheat varieties with less lodging. A mutant DD399 with a reduced height phenotype was found to have a 36 Mb terminal deletion in chromosome 2BL.
Article
Plant Sciences
Yang Liu, Xiaomei Fang, Tian Tang, Yudong Wang, Yinhuan Wu, Jinyu Luo, Haotian Wu, Yingqian Wang, Jian Zhang, Renwu Ruan, Meiliang Zhou, Kaixuan Zhang, Zelin Yi
Summary: This study sequenced the transcriptome of common buckwheat and Ukrainian daliqiao using high-throughput sequencing technology, obtaining a large amount of gene information and differentially expressed genes. SSR and EST-SSR loci were also detected. The clustering results verified the genetic relationship between common buckwheat and Ukrainian daliqiao. These findings are important for enriching the genetic information and marker-assisted selection breeding of buckwheat.
Article
Entomology
Sachi Kimura, Yusuke Shibata, Takao Oi, Kazuhito Kawakita, Daigo Takemoto
Summary: Research suggests that flutianil primarily affects haustoria, impacting the morphology of powdery mildew fungi.
JOURNAL OF PESTICIDE SCIENCE
(2021)
Article
Agronomy
Habteab Goitom Gebremedhin, Yahui Li, Jinghuang Hu, Dan Qiu, Qiuhong Wu, Hongjun Zhang, Li Yang, Yang Zhou, Yijun Zhou, Zhiyong Liu, Peng Zhang, Hongjie Li
Summary: This study developed molecular markers for assisted breeding of wheat with powdery mildew resistance. These markers have important applications in wheat breeding and can enhance the efficiency of selecting disease-resistant wheat cultivars.
Article
Plant Sciences
Minghui Zhao, Bei Su, Xiaoxu Zhang, Xiaomei Zhang, Ruobing Li, Peng Cheng, Baotong Wang, Qiang Li
Summary: Wheat variety Changfeng 75 shows high resistance to wheat stripe rust caused by Puccinia striiformis f. sp. tritici. The resistance is controlled by a single recessive gene, YrCf75, located on wheat chromosome 2AL. This finding contributes to better understanding the genetic mechanisms of resistance to wheat stripe rust.
Letter
Biochemistry & Molecular Biology
Xiaocui Yan, Miaomiao Li, Peipei Zhang, Guihong Yin, Huaizhi Zhang, Takele Weldu Gebrewahid, Jianping Zhang, Lingli Dong, Daqun Liu, Zhiyong Liu, Zaifeng Li
Article
Agronomy
Yinghui Li, Zhen-Zhen Wei, Andrii Fatiukha, Samidha Jaiwar, Hanchao Wang, Samiha Hasan, Zhiyong Liu, Hanan Sela, Tamar Krugman, Tzion Fahima
Summary: This study identified the TdPm60 alleles from wild emmer wheat as a strong candidate for PmG16 mildew resistance, which showed improved resistance in Israeli modern bread wheat. The findings suggest that TdPm60 could also be a candidate for other powdery mildew resistance genes.
THEORETICAL AND APPLIED GENETICS
(2021)
Article
Biochemistry & Molecular Biology
Qiuhong Wu, Yongxing Chen, Beibei Li, Jing Li, Panpan Zhang, Jingzhong Xie, Huaizhi Zhang, Guanghao Guo, Ping Lu, Miaomiao Li, Keyu Zhu, Wenling Li, Tzion Fahima, Eviatar Nevo, Hongjie Li, Lingli Dong, Zhiyong Liu
Summary: A new allele of the powdery mildew resistance gene Pm60 was identified in wild emmer wheat, which provides valuable genetic resources for breeding resistant wheat cultivars.
JOURNAL OF GENETICS AND GENOMICS
(2022)
Article
Multidisciplinary Sciences
Shengnan Li, Dexing Lin, Yunwei Zhang, Min Deng, Yongxing Chen, Bin Lv, Boshu Li, Yuan Lei, Yanpeng Wang, Long Zhao, Yueting Liang, Jinxing Liu, Kunling Chen, Zhiyong Liu, Jun Xiao, Jin-Long Qiu, Caixia Gao
Summary: Disruption of susceptibility genes in crops can confer disease resistance, but often leads to undesired effects. This study identifies a mutant wheat variety with a targeted deletion in a specific gene that retains crop growth and yields while conferring robust resistance to powdery mildew.
Article
Agronomy
Keyu Zhu, Miaomiao Li, Haibin Wu, Deyun Zhang, Lingli Dong, Qiuhong Wu, Yongxing Chen, Jingzhong Xie, Ping Lu, Guanghao Guo, Huaizhi Zhang, Panpan Zhang, Beibei Li, Wenling Li, Lei Dong, Qifei Wang, Jinghuan Zhu, Wenli Hu, Liqiao Guo, Rongge Wang, Chengguo Yuan, Hongjie Li, Zhiyong Liu, Wei Hua
Summary: In this study, the powdery mildew resistance gene MlWE74 from wild emmer wheat accession G-748-M was mapped in an NBS-LRR gene cluster on chromosome 2BS. The study revealed that the resistance in wheat line WE74 is controlled by a single dominant gene, MlWE74, and identified molecular markers linked to this gene. Additionally, several candidate genes were identified for further investigation in map-based cloning of MlWE74.
THEORETICAL AND APPLIED GENETICS
(2022)
Article
Multidisciplinary Sciences
Xiaoyu Zhang, Haiyan Jia, Tian Li, Jizhong Wu, Ragupathi Nagarajan, Lei Lei, Carol Powers, Chia-Cheng Kan, Wei Hua, Zhiyong Liu, Charles Chen, Brett F. Carver, Liuling Yan
Summary: This study reports the influence of spike architecture on grain yield in wheat and successfully cloned the gene TaCOL-B5 that determines the number of spikelet nodes per spike in common wheat. The overexpression of TaCol-B5 increases the spikelet nodes, tillers, and spikes, thereby enhancing grain yield. The allelic variation in TaCOL-B5 affects protein phosphorylation by TaK4.
Article
Plant Sciences
Elena Salina, Alexander Muterko, Antonina Kiseleva, Zhiyong Liu, Abraham Korol
Summary: This research identified two regions on wheat chromosome 5B that cause recombination suppression, located on the short arm and long arm. Comparative analysis of different genetic maps revealed that chromosomal rearrangements and the activity of genetic elements contribute to the recombination suppression, which may be related to speciation and genetic adaptation.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Miaomiao Li, Lei Dong, Keyu Zhu, Qiuhong Wu, Yongxing Chen, Ping Lu, Guanghao Guo, Huaizhi Zhang, Panpan Zhang, Beibei Li, Wenling Li, Yijun Yang, Yikun Hou, Xuejia Cui, Hongjie Li, Lingli Dong, Yusheng Zhao, Zhiyong Liu
PLANT BIOTECHNOLOGY JOURNAL
(2022)
Article
Plant Sciences
Dan Qiu, Jiang Huang, Guanghao Guo, Jinghuang Hu, Yahui Li, Hongjun Zhang, Hongwei Liu, Li Yang, Yang Zhou, Benzhou Yang, Yudan Zhang, Zhiyong Liu, Hongjie Li
Summary: Wheat genotypes resistant to powdery mildew have been developed and characterized in this study. Two near-isogenic lines were created, one with resistance to the disease and the other susceptible. Genetic and molecular marker analysis revealed that the resistance was associated with a specific gene locus, Pm5e. The two lines showed similar genomic compositions and performed similarly in yield-related and plant growth traits, indicating that Pm5e is a promising disease resistance gene in wheat breeding.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Qiuhong Wu, Yu Cui, Xuejiao Jin, Guoxin Wang, Lijie Yan, Chenchen Zhong, Meihua Yu, Wenli Li, Yong Wang, Ling Wang, Hao Wang, Chen Dang, Xinyu Zhang, Yongxing Chen, Panpan Zhang, Xiaofei Zhao, Jiajie Wu, Daolin Fu, Lanqin Xia, Eviatar Nevo, John Vogel, Naxin Huo, Dawei Li, Yong Q. Gu, Andrew O. Jackson, Yongliang Zhang, Zhiyong Liu
Summary: In this study, researchers isolated a resistance gene called barley stripe resistance 1 (BSR1) from the Brachypodium distachyon Bd3-1 inbred line using map-based cloning. They found that BSR1, a member of the nucleotide binding, leucine-rich repeat immune receptor (NLR) protein family, confers resistance to Barley stripe mosaic virus (BSMV) in monocots. Protein domain swaps revealed that the intact leucine-rich repeat (LRR) domain and the C-terminus of BSR1 are essential for resistance. BSR1 interacts with BSMV ND18 TGB1 protein in plant and exhibits temperature-sensitive antiviral resistance.
Review
Biology
Jun Xiao, Bao Liu, Yingyin Yao, Zifeng Guo, Haiyan Jia, Lingrang Kong, Aimin Zhang, Wujun Ma, Zhongfu Ni, Shengbao Xu, Fei Lu, Yuannian Jiao, Wuyun Yang, Xuelei Lin, Silong Sun, Zefu Lu, Lifeng Gao, Guangyao Zhao, Shuanghe Cao, Qian Chen, Kunpu Zhang, Mengcheng Wang, Meng Wang, Zhaorong Hu, Weilong Guo, Guoqiang Li, Xin Ma, Junming Li, Fangpu Han, Xiangdong Fu, Zhengqiang Ma, Daowen Wang, Xueyong Zhang, Hong-Qing Ling, Guangmin Xia, Yiping Tong, Zhiyong Liu, Zhonghu He, Jizeng Jia, Kang Chong
Summary: This review summarizes recent advances in genomic research and genetic improvement of bread wheat, with a focus on the contributions made by Chinese scientists. The availability of multiple reference wheat genome assemblies and cutting-edge technologies such as precise genome editing tools has opened up new possibilities for functional genomic research in wheat. Insights gained from these advances will enhance our understanding of the molecular mechanisms and regulatory networks underlying agronomic traits, and facilitate the development of modern breeding techniques for more sustainable agriculture worldwide.
SCIENCE CHINA-LIFE SCIENCES
(2022)
Article
Agronomy
Habteab Goitom Gebremedhin, Yahui Li, Jinghuang Hu, Dan Qiu, Qiuhong Wu, Hongjun Zhang, Li Yang, Yang Zhou, Yijun Zhou, Zhiyong Liu, Peng Zhang, Hongjie Li
Summary: This study developed molecular markers for assisted breeding of wheat with powdery mildew resistance. These markers have important applications in wheat breeding and can enhance the efficiency of selecting disease-resistant wheat cultivars.
Article
Biochemistry & Molecular Biology
Shanying Zhu, Cheng Liu, Shuangjun Gong, Zhaozhao Chen, Rong Chen, Tianlei Liu, Renkang Liu, Haonan Du, Rui Guo, Genying Li, Miaomiao Li, Renchun Fan, Zhiyong Liu, Qian-Hua Shen, Anli Gao, Pengtao Ma, Huagang He
Summary: Wheat powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is a devastating disease that threatens wheat production worldwide. Pm12, originated from Aegilops speltoides, confers strong resistance to powdery mildew and has potential use in wheat breeding. The study demonstrated the physical mapping, isolation, and functional validation of Pm12, which is orthologous to Pm21 from Dasypyrum villosum.
PLANT COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Fei Zhao, Shilong Tian, Qiuhong Wu, Zijuan Li, Luhuan Ye, Yili Zhuang, Meiyue Wang, Yilin Xie, Shenghao Zou, Wan Teng, Yiping Tong, Dingzhong Tang, Ajay Kumar Mahato, Moussa Benhamed, Zhiyong Liu, Yijing Zhang
Summary: This study presents Triti-Map, a computational package and web interface specifically designed for gene mapping in Triticeae. The authors successfully located a wheat disease-resistance gene and clarified its evolutionary process using Triti-Map. This tool has the potential to facilitate gene isolation and accelerate Triticeae breeding.
PLANT COMMUNICATIONS
(2022)
Article
Agronomy
Yahui Li, Ruiming Lin, Jinghuang Hu, Xiaohan Shi, Dan Qiu, Peipei Wu, Gebremedhin Habteab Goitom, Siqi Wang, Hongjun Zhang, Li Yang, Hongwei Liu, Qiuhong Wu, Jingzhong Xie, Yang Zhou, Zhiyong Liu, Hongjie Li
Summary: A new stripe rust resistance gene called Yr041133 was identified in winter wheat line 041133, which exhibited resistance to multiple pathotypes of the disease.