Article
Parasitology
Shih-Che Weng, Po-Nien Tsao, Shin-Hong Shiao
Summary: This study found that blood glucose enhances DENV replication in mosquitoes, leading to increased virus transmission. The activation of AKT and TOR signaling pathways plays a crucial role in facilitating this process. This insight may contribute to understanding the relationship between dengue fever and diabetes mellitus and could potentially offer new targets for antiviral therapies.
PARASITES & VECTORS
(2021)
Article
Parasitology
Lie Cheng, Wei-Liang Liu, Matthew P. Su, Shu-Chen Huang, Jen-Ren Wang, Chun-Hong Chen
Summary: In this study, we found that prohemocytes were the major type of hemocyte infected by DENV in both Ae. aegypti and Ae. albopictus. The infection rate of prohemocytes in Ae. albopictus was lower than that in Ae. aegypti, which may explain why systemic DENV infection in Ae. albopictus is less efficient than in Ae. aegypti and why Ae. albopictus is less correlated to dengue fever outbreaks.
PARASITES & VECTORS
(2022)
Article
Biochemistry & Molecular Biology
Chun -Ting Yeh, Shih-Che Weng, Po-Nien Tsao, Shin -Hong Shiao
Summary: Binding immunoglobulin protein (BiP) plays a crucial role in both dengue virus replication and mosquito reproduction. Silencing BiP expression inhibits viral replication and reduces mosquito fertility and fecundity.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2023)
Article
Medicine, General & Internal
Triwibowo Ambar Garjito, Lulus Susanti, Mujiyono Mujiyono, Mega Tyas Prihatin, Dwi Susilo, Sidiq Setyo Nugroho, Mujiyanto Mujiyanto, Raden Ajeng Wigati, Tri Baskoro Tunggul Satoto, Sylvie Manguin, Laurent Gavotte, Roger Frutos
Summary: There are various methods to collect and assess the abundance of dengue vector mosquitoes, but the lack of long-term sampling methods may result in variability and lack of statistical significance in the results. There is no correlation between the collection methods and the presence of dengue virus in mosquitoes or the incidence of dengue, and the efficacy of a given method is inconsistent across different locations.
FRONTIERS IN MEDICINE
(2021)
Article
Parasitology
Daniel Chieh-Ding Lin, Shih-Che Weng, Po-Nien Tsao, Justin Jang Hann Chu, Shin-Hong Shiao
Summary: Co-infection of dengue virus and Zika virus in Aedes aegypti mosquitoes enhance viral replication and suggest that the NS5 protein is a potential target for vector control.
PARASITES & VECTORS
(2023)
Article
Microbiology
Michael Leitner, Cameron Bishop, Sassan Asgari
Summary: The study revealed the transcriptional response of Wolbachia in A. aegypti cells infected with dengue virus, indicating changes in the expression of certain functionally important Wolbachia genes. This provides insights into the endosymbiont's reaction to virus infection.
Article
Microbiology
Shih-Che Weng, Shin-Hong Shiao
Summary: This study investigated the impact of protein SUMOylation on dengue virus replication in A. aegypti. The findings suggest that protein SUMOylation plays an important role in dengue virus replication and transmission.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Medicine, General & Internal
A. Utarini, C. Indriani, R. A. Ahmad, W. Tantowijoyo, E. Arguni, M. R. Ansari, E. Supriyati, D. S. Wardana, Y. Meitika, I. Ernesia, I. Nurhayati, E. Prabowo, B. Andari, B. R. Green, L. Hodgson, Z. Cutcher, E. Rances, P. A. Ryan, S. L. O'Neill, S. M. Dufault, S. K. Tanamas, N. P. Jewell, K. L. Anders, C. P. Simmons
Summary: The cluster-randomized trial in Indonesia showed that deploying mosquitoes infected with the wMel strain of Wolbachia pipientis led to a reduction in symptomatic, virologically confirmed dengue infections and hospitalizations among residents.
NEW ENGLAND JOURNAL OF MEDICINE
(2021)
Article
Biotechnology & Applied Microbiology
Michael Leitner, Kayvan Etebari, Sassan Asgari
Summary: This study examines the transcriptional response of Wolbachia-transinfected Aedes aegypti cells to dengue virus (DENV) infection. Differential expression of immune genes, as well as genes related to post-transcriptional modifications, antioxidant enzymes, and heat-shock response, are identified. Additionally, several long non-coding RNAs show differential regulation. These findings provide insights into the initial virus recognition and transcriptional response of Wolbachia-transinfected Aedes aegypti to DENV infection.
JOURNAL OF GENERAL VIROLOGY
(2022)
Article
Multidisciplinary Sciences
Shailabh Kumar, Felix J. H. Hol, Sujit Pujhari, Clayton Ellington, Haripriya Vaidehi Narayanan, Hongquan Li, Jason L. Rasgon, Manu Prakash
Summary: The Vectorchip is a low-cost microfluidic platform that enables high-throughput analysis of individual mosquito bites, allowing detection of mosquito-carried DNA, RNA, and viral particles. This technology provides a promising approach for laboratory and field characterization of vector-pathogen communities.
NATURE COMMUNICATIONS
(2021)
Article
Entomology
Maria E. Santana-Roman, Paola Maycotte, Salvador Uribe-Carvajal, Cristina Uribe-Alvarez, Nayeli Alvarado-Medina, Mohsin Khan, Aleem Siddiqui, Victoria Pando-Robles
Summary: Dengue is a growing public health concern without specific treatment, and vector control remains crucial for prevention. Studies show that DENV infection alters mitochondrial physiology in mosquito cells, affecting redox metabolism and membrane potential. The maintenance of mitochondrial function through fusion may play a vital role in the DENV-infected cells.
Review
Immunology
Zhuanzhuan Liu, Qingxin Zhang, Liya Li, Junjie He, Jinyang Guo, Zichen Wang, Yige Huang, Zimeng Xi, Fei Yuan, Yiji Li, Tingting Li
Summary: This review explores the correlations between temperature and dengue epidemics, including the distribution and development of Aedes mosquitoes, the structure of DENV, and the vector competence of Aedes mosquitoes. Additionally, it discusses the immune and metabolic mechanism through which temperature affects the vector competence of Aedes mosquitoes to transmit DENV.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
L. L. Lima, A. P. F. Atman
Summary: In an increasingly interconnected society, preventing epidemics has become a major challenge. Using an agent-based model, the authors investigated the network morphology of a dengue endemic region considering different serotypes and a small population. The simulation results indicate that the spread of dengue is primarily driven by human-to-human and human-to-mosquito interaction, reinforcing the importance of controlling the vector to prevent episodes of epidemic outbreaks.
Article
Virology
Mazhar Hussain, Kayvan Etebari, Sassan Asgari
Summary: Wolbachia pipientis can block replication of positive sense RNA viruses. A study on Aedes aegypti cell lines revealed that dengue virus (DENV) was blocked in cells carrying the wAlbB strain of Wolbachia, but significantly inhibited in tetracycline-cured cells. RNA-Seq analysis showed the removal of Wolbachia and absence of Wolbachia gene expression in tetracycline-cured cells, while the abundance of phasi charoen-like virus (PCLV) was increased. Decreasing PCLV levels through RNAi resulted in increased DENV replication, indicating an antagonistic interaction between DENV and PCLV.
Article
Multidisciplinary Sciences
Shinji Kasai, Kentaro Itokawa, Nozomi Uemura, Aki Takaoka, Shogo Furutani, Yoshihide Maekawa, Daisuke Kobayashi, Nozomi Imanishi-Kobayashi, Michael Amoa-Bosompem, Katsunori Murota, Yukiko Higa, Hitoshi Kawada, Noboru Minakawa, Tran Chi Cuong, Nguyen Thi Yen, Tran Vu Phong, Sath Keo, Kroesna Kang, Kozue Miura, Lee Ching Ng, Hwa-Jen Teng, Samuel Dadzie, Sri Subekti, Kris Cahyo Mulyatno, Kyoko Sawabe, Takashi Tomita, Osamu Komagata
Summary: Aedes aegypti, the main mosquito vector for dengue and other arboviral infectious diseases, has developed high levels of pyrethroid resistance in populations collected from Vietnam and Cambodia. This resistance could pose a serious threat to the control of dengue fever and other Aedes-borne infectious diseases.
Article
Chemistry, Multidisciplinary
Lifu Xiao, Chuanqi Wang, Chen Dai, Laurie E. Littlepage, Jun Li, Zachary D. Schultz
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2020)
Article
Biochemical Research Methods
Chuanqi Wang, Jun Li
Article
Biology
David Kang, Angela E. Douglas
Article
Multidisciplinary Sciences
Chuanqi Wang, Lifu Xiao, Chen Dai, Anh H. Nguyen, Laurie E. Littlepage, Zachary D. Schultz, Jun Li
SCIENTIFIC REPORTS
(2020)
Article
Multidisciplinary Sciences
Gabriel Wright, Anabel Rodriguez, Jun Li, Patricia L. Clark, Tijana Milenkovic, Scott J. Emrich
Article
Entomology
David S. Kang, Joanne M. Cunningham, Diane D. Lovin, Dave D. Chadee, David W. Severson
JOURNAL OF MEDICAL ENTOMOLOGY
(2020)
Article
Cell Biology
Hao Wang, Wei Hou, Aldeb Perera, Carlee Bettler, Jordan R. Beach, Xianzhong Ding, Jun Li, Mitchell F. Denning, Asha Dhanarajan, Scott J. Cotler, Cara Joyce, Jun Yin, Fowsiyo Ahmed, Lewis R. Roberts, Wei Qiu
Summary: EphA2 expression is enriched in human HCC and loss of EphA2 can suppress the initiation and growth of HCC. Targeting EphA2 inhibits both AKT and JAK1/STAT3 signaling pathways in HCC, and a small molecule kinase inhibitor of EphA2 shows promising results in suppressing tumor progression in a murine model.
Article
Pharmacology & Pharmacy
Md Sazzad Hassan, Nicholas Cwidak, Chloe Johnson, Silvio Daester, Serenella Eppenberger-Castori, Niranjan Awasthi, Jun Li, Margaret A. Schwarz, Urs von Holzen
Summary: Elevated c-Myc expression in esophageal cancer cells is associated with accelerated tumor growth, and targeting c-Myc with the CDK inhibitor flavopiridol can selectively kill these aggressive cancer cells. Combination therapy with flavopiridol and other agents shows promise in inhibiting cancer cell proliferation and improving overall survival in c-Myc overexpressing esophageal cancer.
FRONTIERS IN PHARMACOLOGY
(2021)
Review
Entomology
Cynthia L. Goodman, David S. Kang, David Stanley
Summary: Cell lines are valuable tools for studying insect and tick immunity, allowing researchers to investigate how these organisms recognize and respond to infections, as well as develop strategies for future research. In particular, immortal arthropod cell lines provide a cost-effective and consistent platform for understanding and controlling important insects and ticks.
Article
Cell Biology
Shant M. Mahserejian, Jared P. Scripture, Ava J. Mauro, Elizabeth J. Lawrence, Erin M. Jonasson, Kristopher S. Murray, Jun Li, Melissa Gardner, Mark Alber, Marija Zanic, Holly V. Goodson
Summary: This article introduces a statistical tool called STADIA for automated dynamic instability analysis of microtubules (MTs). It demonstrates that stutters, which are intermediate behaviors during MT growth and shortening, precede most catastrophes, suggesting their mechanistic involvement in catastrophes. The study also reveals that the anticatastrophe factor CLASP2 promotes the return of stuttering MTs to growth. STADIA enables a more comprehensive and data-driven analysis of MT dynamics and provides new opportunities for studying the mechanisms and regulation of MT behavior.
MOLECULAR BIOLOGY OF THE CELL
(2022)
Article
Biochemistry & Molecular Biology
Gabriel Wright, Anabel Rodriguez, Jun Li, Tijana Milenkovic, Scott J. Emrich, Patricia L. Clark
Summary: Research has shown that synonymous codon usage can affect various mechanisms related to protein production, especially in co-translational protein folding. Conservation of synonymous codon usage patterns across evolution highlights the potential benefits of matching codon usage patterns from the original organism in heterologous gene expression.
Article
Biochemistry & Molecular Biology
Khalique Newaz, Jacob Piland, Patricia L. Clark, Scott J. Emrich, Jun Li, Tijana Milenkovic
Summary: This study presents a novel approach for protein structural classification by modeling protein 3D structures as multi-layer sequential PSNs to capture 3D sub-structures. The results show that this approach outperforms existing methods.
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS
(2022)
Article
Biochemical Research Methods
Dailin Gan, Jun Li
Summary: SCIBER is a new batch-effect-removal method that can remove batch effects before integrating multiple single-cell RNA-sequencing datasets to avoid misleading interpretations. It has better scalability and tunability compared to existing methods, and it can output expression data of individual genes for downstream analyses.
Article
Computer Science, Artificial Intelligence
Zixuan Song, Jun Li
Summary: SurvNet is a backward elimination procedure for variable selection in deep neural networks, with the ability to estimate and control the false discovery rate of selected variables. It adaptively determines how many variables to eliminate at each step to maximize selection efficiency.
NATURE MACHINE INTELLIGENCE
(2021)
Article
Biotechnology & Applied Microbiology
Hongyu Guo, Jun Li
Summary: scSorter is a new method developed for assigning cells to known cell types on single-cell RNA-sequencing data, showing much higher power compared to existing methods on simulated and real data.