Article
Chemistry, Analytical
Alan B. Moran, Richard A. Gardner, Manfred Wuhrer, Guinevere S. M. Lageveen-Kammeijer, Daniel I. R. Spencer
Summary: Sialic acids play diverse biological roles and linkage-specific analysis is important in glycomic studies. The combination of procainamide fluorescent labeling with sialic acid linkage-specific derivatization is presented, which allows for unambiguous assignment of sialic acid linkages and separation of sialylated isomers.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Sakshi Gautam, Alireza Banazadeh, Byeong Gwan Cho, Mona Goli, Jieqiang Zhong, Yehia Mechref
Summary: Researchers successfully achieved efficient separation of glycan isomers using mesoporous graphitized carbon (MGC) material in a 1 cm-long column, showing reproducibility and stability in both capillary columns and packed nanospray emitters.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Ting Huang, Mateusz Staniak, Felipe da Veiga Leprevost, Amanda M. Figueroa-Navedo, Alexander R. Ivanov, Alexey I. Nesvizhskii, Meena Choi, Olga Vitek
Summary: Repeated measures experimental designs are commonly used in mass spectrometry-based proteomics to quantify proteins in biological subjects repeatedly over multiple conditions or times. Combining repeated measures with tandem mass tag (TMT) labeling presents statistical challenges due to unique interplays of variation. This manuscript proposes a family of linear mixed-effects models for analyzing proteomics experiments with repeated measures and TMT multiplexing, which decompose the variation and enable statistical inference of differential protein abundance.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Chemistry, Analytical
Francis Berthias, Dale A. Cooper-Shepherd, Frederik H. V. Holck, James I. Langridge, Ole N. Jensen
Summary: The combination of cyclic ion mobility spectrometry (cIM) and electron capture dissociation (ECD) allows for the complete separation and sequencing of large isomeric peptides, providing a new method for identifying biologically important proteoforms in complex mixtures.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Ernesto de Jesus Zapata Flores, Nguyen Kim Ngan Bui, Sigrid Selberg, Koit Herodes, Ivo Leito
Summary: This study proposes derivatization reagents based on azobenzene, AzoB and AzoC, for LC-ESI-MS analysis of free amino acids in fermented beverages and juices. The results show that AzoB can be used for targeted and non-targeted analysis, while AzoC has advantages such as fast reaction and good reactivity with secondary amines but is affected by side products for quantitation.
Article
Chemistry, Multidisciplinary
Jyoti Kannoujia, Devendra Nagineni, Ramesh Rodda, Ramesh Chilukuri, Jagadeesh Babu Nanubolu, Parameswari Akshinthala, Soujanya Yarasi, Srinivas Kantevari, Prabhakar Sripadi
Summary: Identification and characterization of impurities in agrochemicals are crucial for their efficacy and public health safety. In this study, we identified and characterized an impurity, 5-chloro-2-cyano-N,N-dimethyl-4-p-tolylimidazole-1-sulfonamide (2), in the fungicide cyazofamid. HPLC analysis suggested that 2 is formed during the reaction of substituted imidazole (1) with N,N-dimethylsulfamoyl chloride. Preparative HPLC isolation and characterization by various techniques confirmed that 2 is an isomer of cyazofamid with the N,N-dimethyl sulfonamide group positioned on the other nitrogen of imidazole near the chloride group. Computational studies further supported the formation of 2 and ruled out other possible isomeric structures.
CHEMISTRY-AN ASIAN JOURNAL
(2023)
Article
Agriculture, Dairy & Animal Science
Jiale Sui, Jing Xiao, Xinping Chang, Yisha Xu, Jinqiu Wang, Fang Geng
Summary: Chicken egg yolk granules (EYG) are rich in proteins, phospholipids, and minerals compared to egg yolk. Proteomic analysis of EYG revealed the identification of phosphorylation and N-glycosylation sites on various proteins, providing new insights into the structure and function of EYG. This study contributes to the development and utilization of EYG.
Article
Plant Sciences
Mafalda Pinto, Cristiano Soares, Ruth Pereira, Jose Antonio Rodrigues, Fernanda Fidalgo, Ines Maria Valente
Summary: Aqueous extracts from Eucalyptus globulus leaves contain a wide variety of specialized metabolites with diverse biological activities. Previous studies have mainly focused on extracts from mature leaves, while the composition of extracts from young eucalyptus leaves is still unclear. There is also no consensus on whether fresh or dried leaves should be used for extraction to obtain the highest recovery of biologically active compounds.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Chemistry, Applied
Ozge Aksay, Serkan Selli, Hasim Kelebek
Summary: The study aimed to compare the bioactive compounds and quality parameters of fresh and fermented caper buds and berries, finding that the phenolic content and antioxidant capacity were higher in fresh and fermented buds than in berries. The amounts of isorhamnetin, quercetin, and kaempferol increased in fermented samples, while total phenolics and antioxidant properties were better preserved in dry-salted samples compared to brined samples.
Article
Food Science & Technology
Maria Eugenia Araujo Silva Oliveira, Luciana Ribeiro da Silva Lima, Millena Cristina Barros Santos, Nathalia Ferrari Fonseca de Sales, Renata Marenda Ferreira, Luiz Claudio Cameron, Jose Manoel Colombari Filho, Priscila Zaczuk Bassinello, Carlos Wanderlei Piler de Carvalho, Mariana Simoes Larraz Ferreira, Cristina Yoshie Takeiti
Summary: This study investigated the effects of short germination and milling on the physical properties, phenolic compounds, antioxidant activity, and amino acid composition of rice. The results showed that short germination and milling can improve the nutritional quality and antioxidant activity of rice. The husk + bran fraction had the highest level of total antioxidant activity, and the levels of glutamic acid and GABA were significantly increased. Short germination and milling can be an effective strategy to enhance the nutritional value of rice.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Pharmacology & Pharmacy
Hui Wang, Jian-Ying Dai, Yu-Zhen He, Zhe-Wei Xia, Xiao-Fei Chen, Zhan-Ying Hong, Yi-Feng Chai
Summary: This study examined the therapeutic effect and mechanism of Anemarrhenae Rhizoma (AR) on AD model rats using serum metabolomics. The results showed that AR significantly affected the levels of multiple metabolites involved in various metabolic pathways in the serum of AD model rats. These findings were consistent with the results of behavior study, histopathological observations, and biochemical analyses, providing a scientific basis for the application of AR in the prevention and treatment of AD.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Food Science & Technology
Yue Jia, Yu Lu, Xue Wang, Yuerong Yang, Meiyi Zou, Jie Liu, Wanjun Jin, Xiaoqin Wang, Guoliang Pang, Linjuan Huang, Zhongfu Wang
Summary: The study found that with prolonged lactation, the relative content of glycan derivatives of bovine lactoferrin changed, with the highest content observed in colostrum. Additionally, linkage-specific derivatization of sialic acid could distinguish between different sialylated N-glycan isomers.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Environmental Sciences
Zhiheng Wang, Jingyi Zhang, Lingzhi Zhang, Yongmei Liang, Quan Shi
Summary: The study collected PM2.5 samples in Beijing and characterized atmospheric NACs for the first time using LC-Orbitrap MS technology, identifying and quantifying 165 out of 190 NACs, as well as nitro-polyaromatic compounds. Nitrophenols and nitrocatechols were the most abundant NACs species, accounting for 31% and 32% of total NACs, respectively. The study also found that NACs had a significant contribution to organic matter and PM2.5 in winter, with major sources identified as coal combustion, biomass combustion, dust, and transportation.
ATMOSPHERIC ENVIRONMENT
(2021)
Article
Biochemistry & Molecular Biology
Zhilin Hao, Li Liang, He Liu, Yi Yan, Yuyu Zhang
Summary: Daylily is a valuable plant resource with various health benefits. In this study, four extraction methods were compared for their effectiveness in extracting phenolic compounds from daylily. The ultrasonic-assisted ethanol extraction method showed the highest total phenolic content and the best antioxidant activity. A total of 26 phenolic compounds were identified, with the ultrasonic-assisted ethanol extract showing a higher abundance of phenolic compounds. These results provide theoretical support for the extraction of phenolic compounds from daylily and its application as a functional food.
Article
Plant Sciences
Yingting Zhang, Liwei Yang, Junjie Yang, Hailiang Hu, Guangqian Wei, Jiebing Cui, Jin Xu
Summary: The metabolite contents of Chinese cedar needles vary across different seasons. This study performed integrated non-targeted metabolomic and transcriptomic analyses to uncover the biosynthetic mechanisms of differentially synthesized metabolites (DSMs) in Chinese cedar needles. The results provide valuable insights into the adaptive mechanisms and extraction of metabolites in Chinese cedar.
FRONTIERS IN PLANT SCIENCE
(2022)
Editorial Material
Plant Sciences
Ashwell Rungano Ndhlala, Ntakadzeni Edwin Madala, Bhekumthetho Ncube
SOUTH AFRICAN JOURNAL OF BOTANY
(2020)
Article
Multidisciplinary Sciences
Ndamulelo Nengovhela, Paul Anton Steenkamp, Ntakadzeni Edwin Madala
Summary: Flavonoids, plant metabolites, exhibit diverse chemical modifications such as glycoisomerization and sugar acylation in different plant species. These chemical modifications are utilized by plants as an evolutionary strategy to diversify their metabolome compositions.
NATIONAL ACADEMY SCIENCE LETTERS-INDIA
(2021)
Article
Plant Sciences
Anza-Tshilidzi Ramabulana, Paul A. Steenkamp, Ntakadzeni E. Madala, Ian A. Dubery
Article
Chemistry, Physical
Ntakadzeni E. Madala, Mwadham M. Kabanda
Summary: An experimental isolation of clovamide molecule from Vernonia fastigiata was conducted, and its E and Z geometrical isomers were characterized using liquid chromatography-mass spectrometry. The most thermodynamically stable isomer was determined to be the E isomer. Clovamide exhibited stronger (OH)• scavenging ability in non-polar medium compared to (OOH)•, and the preferred mechanism of radical scavenging was hydrogen atom transfer.
JOURNAL OF MOLECULAR STRUCTURE
(2021)
Article
Horticulture
Maanea L. Ramphinwa, Godwin R. A. Mchau, Ntakadzeni E. Madala, Ndamulelo Nengovhela, John B. O. Ogola, Fhatuwani N. Mudau
Summary: This study aims to determine the effects of selected shade nets and seasonal variation on plant growth and development, and hydroxycinnamic acid content of field-grown bush tea. The results showed that the use of shade nets resulted in reduced plant growth and yield, while the accumulation of hydroxycinnamic acid was higher in 80% white shade net plots.
Article
Biochemistry & Molecular Biology
Anza-Tshilidzi Ramabulana, Daniel Petras, Ntakadzeni E. Madala, Fidele Tugizimana
Summary: This study utilized molecular networking to explore the chemical composition of four Momordica plant species, revealing a variety of compound classes such as flavonoids, terpenoids, and lipids. Computational tools were used to analyze and classify the biologically active metabolites within these plants, demonstrating their phytochemical richness and differential distribution across species.
Article
Biochemical Research Methods
X. E. Mabasa, L. M. Mathomu, N. E. Madala, E. M. Musie, M. T. Sigidi
Summary: This study analyzed bioactive compounds in the methanolic extract of Momordica balsamina leaves using molecular spectroscopic and chromatographic techniques, revealing the presence of flavonoids, terpenoids, and other secondary metabolites. The extract showed anti-inflammatory activity and was non-toxic in vitro, indicating the potential health benefits of these compounds. This research provides scientific evidence for the anti-inflammatory properties of Momordica balsamina leaves.
BIOCHEMISTRY RESEARCH INTERNATIONAL
(2021)
Article
Biochemistry & Molecular Biology
Babra Moyo, Nikita Tawanda Tavengwa, Ntakadzeni Edwin Madala
Summary: This study revealed the effect of UV radiation on the CGA composition of Viscum combreticola Engl. (Santalacaeae) plants. The results showed that these plants have a diverse chemical composition of CGAs, including cis and trans isomers, which can help them withstand UV-induced oxidative stress and have potential pharmacological properties.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
(2022)
Article
Plant Sciences
Dakalo Terrence Mbedzi, Lutendo Michael Mathomu, Msizi Innocent Mhlongo, Ntakadzeni Edwin Madala
SOUTH AFRICAN JOURNAL OF BOTANY
(2022)
Article
Plant Sciences
Khuliso Mathatha, Adivhaho Khwathisi, Anza-Tshilidzi Ramabulana, Imah Mwaba, Lutendo Michael Mathomu, Ntakadzeni Edwin Madala
Summary: Bidens pilosa is an edible plant with highly sought-after nutraceutical properties. This study reports the putative identification of genes related to the accumulation of metabolites in B. pilosa, providing a basis for further research on its nutraceutical values.
SOUTH AFRICAN JOURNAL OF BOTANY
(2022)
Article
Engineering, Chemical
Tshimangadzo Nesane, Ntakadzeni E. Madala, Mwadham M. Kabanda, Lutendo C. Murulana
Summary: Electrochemical impedance spectroscopy (EIS), potentiodynamic polarization (PDP), and gravimetric analysis were used to evaluate the ability of Lippia javanica leaf extract to inhibit aluminum corrosion in acidic solution. The extract decreased corrosion current density and double-layer capacitance, and formed a protective adsorption layer on the aluminum surface. The maximum protective efficacy was 91.89% at an optimal concentration of 800 ppm. The extract changed the contact angle and revealed the involvement of various functional groups in the adsorption process. Density functional theory (DFT) analysis showed the preferred binding position of the extract on the aluminum surface.
JOURNAL OF ADHESION SCIENCE AND TECHNOLOGY
(2023)
Article
Biochemical Research Methods
Babra Moyo, Yolanda Novokoza, Nikita Tawanda Tavengwa, Nikolai Kuhnert, Kevin Lobb, Ntakadzeni Edwin Madala
Summary: In this study, computational tools and mass spectrometry analysis were used to characterize three viscutin molecules in Viscum combreticola Engl. The results revealed their unique fragmentation patterns and demonstrated that computational tools and theoretical modeling are important in metabolite identification.
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2023)
Article
Chemistry, Analytical
Dakalo Lorraine Ndou, Ashwell Rungano Ndhlala, Nikita Tawanda Tavengwa, Ntakadzeni Edwin Madala
Summary: This study utilized molecular networking and computational tools to investigate the chemical composition of Moringa oleifera leaf extracts, with a focus on flavonoids. The results revealed the presence of various molecular families, including flavonoids, carboxylic acids and derivatives, lignin glycosides, fatty acyls, and macrolactams. The use of computational tools further led to the discovery of structurally diverse flavonoid molecules, which can be attributed to the intricate glycosylation modifications in Moringa oleifera leaves.
JOURNAL OF ANALYTICAL METHODS IN CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Motseoa Mariam Lephatsi, Mpho Susan Choene, Abidemi Paul Kappo, Ntakadzeni Edwin Madala, Fidele Tugizimana
Summary: South Africa is rich in diverse medicinal plants, with over 35% of the global Helichrysum species. However, there are knowledge gaps regarding the size and diversity of the Helichrysum chemical space. This study aimed to comprehensively characterize the metabolic landscape of Helichrysum splendidum, a less studied medicinal herb. The results revealed a chemically diverse metabolic map, with potential bioactive compounds that showed strong interactions with anti-cancer targets. These findings suggest the potential of H. splendidum-derived compounds as anti-cancer agents.
Article
Biochemistry & Molecular Biology
Babra Moyo, Nikita T. Tavengwa, Ntakadzeni E. Madala
Summary: In this study, a metabolic profiling approach and classical molecular networking were used to investigate the differences in polyphenol profiles between Viscum combreticola Engl. and Viscum album L. The results showed that the two species had distinct polyphenol profiles, which could serve as chemotaxonomical markers. Additionally, molecular networking was shown to be a powerful tool for visualizing differences in chemical composition. The diverse polyphenol profiles of these species are likely influenced by their unique metabolism, which is influenced by their genetic makeup.
BIOCHEMICAL SYSTEMATICS AND ECOLOGY
(2023)