Article
Engineering, Biomedical
Robert J. Elkington, Richard M. Hall, Andrew R. Beadling, Hemant Pandit, Michael G. Bryant
Summary: To preserve the function of synovial joint counter-face during osteochondral defect repairs, it is important to develop surfaces optimized for healthy cartilage function. In this study, PEEK surfaces were modified with a hydrophilic coating (SPMK-g-PEEK) to provide a low friction and compliant sliding interface. The results showed that SPMK-g-PEEK maintained low friction and minimized forces on the cartilage interface, providing an enhanced cartilage counter-surface.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2023)
Article
Chemistry, Physical
Hui Yuan, Laura L. E. Mears, Yuefei Wang, Rongxin Su, Wei Qi, Zhimin He, Markus Valtiner
Summary: Osteoarthritis, a common degenerative joint disease, causes serious pain due to the progressive loss and destruction of articular cartilage. Restoring joint lubrication is crucial for its treatment, and various lubricants have been developed to reduce friction and promote cartilage repair. This review discusses the recent progress in natural, bioinspired, and alternative lubricants for osteoarthritis treatment, as well as their structural characterization and lubrication properties.
ADVANCES IN COLLOID AND INTERFACE SCIENCE
(2023)
Article
Engineering, Mechanical
Matus Ranusa, Martin Ondra, David Rebenda, Martin Vrbka, Jiri Gallo, Ivan Krupka
Summary: Lubrication of cartilage is crucial in slowing down the progression of osteoarthritis, and viscosupplementation is a potential therapeutic intervention that can supplement the lubrication ability of cartilage with hyaluronic acid. However, selecting a proper viscosupplement remains a challenge, and its molecular weight may influence the development of the coefficient of friction.
Review
Immunology
James Orman Early, Lauren E. Fagan, Annie M. Curtis, Oran D. Kennedy
Summary: Inflammation is an important biological response to tissue damage, but dysregulation of the process can lead to tissue damage. Mitochondria play a key role in orchestrating the inflammatory response, beyond their traditional function in energy production. Osteoarthritis, previously thought of as non-inflammatory, now appears to involve chronic inflammation as well.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Yanxi Liu, Karan M. Shah, Jian Luo
Summary: Articular cartilage is avascular and has limited ability to repair itself, leading to degenerative joint diseases. Current clinical treatments often result in mechanically inferior cartilage. Understanding factors affecting cartilage homeostasis and exploring emerging regenerative approaches are crucial for future treatment options.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ke Li, Xing Ji, Rebecca Seeley, Wen-Chih Lee, Yu Shi, Fangfang Song, Xueyang Liao, Chao Song, Xiaobin Huang, Danielle Rux, Ju Cao, Xiaoji Luo, Steven M. Anderson, Wei Huang, Fanxin Long
Summary: This study found that the expression of glucose transporter Glut1 is reduced in articular cartilage in osteoarthritis (OA). Inducible deletion or forced expression of Glut1 can accelerate or protect against cartilage destruction, respectively. Moreover, in diabetic mice, both Glut1 expression and glycolysis rate are diminished in articular cartilage, resulting in more severe cartilage destruction.
Article
Biochemistry & Molecular Biology
Phaedra Winstanley-Zarach, Gregor Rot, Shweta Kuba, Aibek Smagul, Mandy J. J. Peffers, Simon R. R. Tew
Summary: Polyadenylation (polyA) defines the 3' boundary of a transcript's genetic information. Alternative polyadenylation (APA) transcripts can exist for a gene, causing variance in regulatory domains and potential coding sequence changes. This study analyzed the polyA site distribution in healthy and osteoarthritic knee articular chondrocytes and found modest differential use of polyadenylation sites between the two conditions, suggesting potential functional relevance in affected genes. This characterization of polyadenylation landscape contributes to understanding the role of polyA in knee osteoarthritis, distinguishing it from other pathologies such as cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Immunology
Taku Saito
Summary: The superficial zone of articular cartilage produces PRG4, which contributes to smooth joint motion. PRG4, besides being an extracellular matrix protein, has novel signaling effects. Various molecules and signaling pathways regulate the expression of PRG4 and the superficial zone. Further research on PRG4-related pathways and cells may uncover the mechanisms underlying joint homeostasis.
INFLAMMATION AND REGENERATION
(2022)
Article
Orthopedics
M. Jarraya, A. Guermazi, J. W. Liew, I. Tolstykh, J. A. Lynch, P. Aliabadi, D. T. Felson, M. Clancy, M. Nevitt, C. E. Lewis, J. Torner, T. Neogi
Summary: The aim of this study was to report the prevalence of CT-detected intra-articular mineralization. It was found that 9.8% of knees had intra-articular mineralization based on CT imaging. Mineralization predominantly occurred in the three compartments of the joint, especially the patellofemoral compartment.
OSTEOARTHRITIS AND CARTILAGE
(2023)
Article
Engineering, Biomedical
Mathaeus Tschaikowsky, Tanja Neumann, Sofia Brander, Heiko Haschke, Bernd Rolauffs, Bizan N. Balzer, Thorsten Hugel
Summary: Understanding the function and dysfunction of hierarchically organized biomaterials or tissues requires studying different length scales. Atomic force microscopy (AFM) combined with fluorescence microscopy can provide detailed information from millimeter to nanometer scales. This approach is not only applicable to cartilage but also useful for characterizing native biological tissues from macro- to nanoscale.
ACTA BIOMATERIALIA
(2021)
Article
Biotechnology & Applied Microbiology
Tianyu Gao, Alexander J. Boys, Crystal Zhao, Kiara Chan, Lara A. Estroff, Lawrence J. Bonassar
Summary: This study utilized confocal Raman microspectroscopy to investigate the degradation of articular cartilage, specifically focusing on spatially mapping the loss of glycosaminoglycans (GAGs), providing a technique with high spatial resolution to measure cartilage degradation without tissue sections.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Orthopedics
Shannon K. Walsh, Rikin Soni, Lisa M. Arendt, Melissa C. Skala, Corinne R. Henak
Summary: The study revealed age-dependent metabolic activity in cartilage, with metabolic dysfunction impacting gene expression related to tissue maintenance. Glycolysis, oxidative phosphorylation activity, and optical redox ratio were positively correlated with severity of cartilage damage.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2022)
Review
Nanoscience & Nanotechnology
Xu Li, Bingyang Dai, Jiaxin Guo, Lizhen Zheng, Quanyi Guo, Jiang Peng, Jiankun Xu, Ling Qin
Summary: The review discusses the pathological features of osteoarthritis, the design concept for accurate drug localization within cartilage, interactions of nanoparticles with cartilage microenvironment, and advances in therapeutic schemes. The emphasis is on developing smart bioresponsive and multiple modality nanoparticles to interact with pathological signals.
NANO-MICRO LETTERS
(2021)
Article
Biochemistry & Molecular Biology
Moon-Chang Choi, Jiwon Jo, Myeongjin Lee, Jonggwan Park, Yoonkyung Park
Summary: The study found that the antimicrobial peptide Cramp induces chondrocyte catabolism via the NF-kappa B signaling pathway, accelerating the progression of OA and leading to cartilage degeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Biomedical
Guillermo Bauza-Mayol, Marcos Quintela, Ava Brozovich, Michael Hopson, Shazad Shaikh, Fernando Cabrera, Aaron Shi, Federica Banche Niclot, Francesca Paradiso, Emma Combellak, Tom Jovic, Paul Rees, Ennio Tasciotti, Lewis W. Francis, Patrick Mcculloch, Francesca Taraballi
Summary: The use of biomimetic scaffolds in cartilage tissue engineering shows promising results in enhancing cartilaginous tissue formation and suppressing host cartilage degeneration. This approach may provide a clinical solution for cartilage tissue repair by modulating the immune environment to favor regenerative processes and suppress cartilage degeneration.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Chemistry, Multidisciplinary
Matteo Romio, Benjamin Grob, Lucca Trachsel, Andrea Mattarei, Giulia Morgese, Shivaprakash N. Ramakrishna, Francesca Niccolai, Elisa Guazzelli, Cristina Paradisi, Elisa Martinelli, Nicholas D. Spencer, Edmondo M. Benetti
Summary: The heterogeneity of side chains in graft polymers significantly influences hydration and hydrophobic interactions, thus impacting fundamental interfacial properties. Side-chain dispersity leads to assemblies that are more hydrated, less adhesive, and more lubricious and biopassive compared to homogeneous structures in graft polymers.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Engineering, Biomedical
Killian Flegeau, Anna Puiggali-Jou, Marcy Zenobi-Wong
Summary: This study evaluated the ability of enzymatically crosslinked hyaluronic acid (HA) microgel bioinks to form mature cartilage in vivo, with promising results achieved through mechanically sizing bulk HA-tyramine hydrogels through meshes of different aperture diameters to form the microgel bioinks.
Article
Materials Science, Biomaterials
Riccardo Rizzo, Angela Bonato, Parth Chansoria, Marcy Zenobi-Wong
Summary: This study presents a simple and scalable method to generate highly porous constructs with aligned guidance cues. By deconstructing a precross-linked hydrogel through a grid, an array of aligned microgels is formed and stabilized by a second photoclick cross-linking step. The method has been optimized both in silico and in vitro, demonstrating excellent viability and organized cellular alignment. Furthermore, proof of concept experiments show that the method can direct aligned muscle tissue maturation.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2022)
Article
Chemistry, Multidisciplinary
Hao Liu, Parth Chansoria, Paul Delrot, Emmanouil Angelidakis, Riccardo Rizzo, Dominic Rutsche, Lee Ann Applegate, Damien Loterie, Marcy Zenobi-Wong
Summary: The FLight biofabrication strategy rapidly creates hydrogels with unidirectional microfilament networks, which can be tuned in diameter and spacing and show outstanding cell instructive properties. This approach allows for the fabrication of complex tissue-engineered constructs with excellent cell guidance.
ADVANCED MATERIALS
(2022)
Review
Biotechnology & Applied Microbiology
Parth Chansoria, Hao Liu, Michael G. Christiansen, Simone Schurle-Finke, Marcy Zenobi-Wong
Summary: Magnetic fields are increasingly being used for remote and noncontact manipulation of cells and biomaterials in regenerative medicine. They can directly affect biological systems or be used in combination with magnetic materials or magnetically tagged cells for targeted therapy. This work highlights the recent trends in using magnetic fields for cell homing, tissue fabrication, and cell fate control. It also discusses the design and control principles of magnetic manipulation systems, guiding future research in developing effective magnetic field-based regenerative strategies.
TRENDS IN BIOTECHNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Dominic Rutsche, Monica Nanni, Simon Rudisser, Thomas Biedermann, Marcy Zenobi-Wong
Summary: This study introduces a new collagen derivative with multiple recognition peptides for enzyme-mediated crosslinking, enabling the co-engineering of human blood and lymphatic microcapillaries. The study further demonstrates the formation of luminal structures and the maturation markers of bioengineered capillaries both in vitro and in vivo. The versatile collagen derivative is also utilized for spatially defined polymer compositions with pro- and anti-angiogenic properties, as well as the formation of vascularization independent from fibroblasts.
ADVANCED MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Riccardo Rizzo, Nika Petelinsek, Angela Bonato, Marcy Zenobi-Wong
Summary: In recent years, the development of novel photocrosslinking strategies and photoactivatable materials has led to widespread use of light-mediated biofabrication techniques. However, current photoresins still rely on photoinitiators (PIs) that produce radicals, potentially causing cytotoxicity. This study presents a radical-free (RF) photocrosslinking strategy based on RF uncaging mechanisms and Michael addition, allowing for high biocompatibility and stability of the constructs. The optimized RF photoresin enables high-resolution two-photon stereolithography (2P-SL) with low polymer concentration, promoting a shift towards radical-free light-based bioprinting.
Article
Engineering, Biomedical
Anna Puiggali-Jou, Maryam Asadikorayem, Katharina Maniura-Weber, Marcy Zenobi-Wong
Summary: Acellular heterogeneous granular hydrogels were developed for promoting cell migration and cartilage tissue maturation. The hydrogels were prepared by mechanical sizing of methacrylated hyaluronic acid and sulfated hyaluronic acid. The addition of growth factor-loaded sulfated microislands guided cell migration and enhanced chondrogenesis.
ACTA BIOMATERIALIA
(2023)
Article
Engineering, Biomedical
Enrico Tosoratti, Angela Bonato, Benjamin Kessel, Patrick Weber, Marcy Zenobi-Wong
Summary: A novel approach for the 3D culture of soft hydrogels is presented, using shape-defining, semi-permeable hydrogel shells to provide mechanical stability. This technique allows for the culture of previously inaccessible materials for tissue engineering and has the potential to change material property requirements in the field.
Editorial Material
Engineering, Biomedical
Daniel J. Kelly, Marcy Zenobi-Wong
Article
Engineering, Biomedical
Angela Bonato, Philipp Fisch, Simone Ponta, David Fercher, Mikko Manninen, Daniel Weber, Kari K. Eklund, Goncalo Barreto, Marcy Zenobi-Wong
Summary: Articular cartilage defects from traumatic injuries are difficult to heal naturally, leading to post-traumatic osteoarthritis. Current cell-based treatments are hindered by limited regenerative capacity and inflammation. Researchers developed a tissue-engineered cartilage that is more resistant to inflammation by using CRISPR-Cas9 to knock out the TAK1 gene in chondrocytes. The engineered cartilage shows successful integration and produces less inflammation-related macrophages, suggesting a promising option for regenerative medicine.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Materials Science, Multidisciplinary
Riccardo Rizzo, Dominic Ruetsche, Hao Liu, Parth Chansoria, Anny Wang, Amelia Hasenauer, Marcy Zenobi-Wong
Summary: Multiscale printing of 3D perfusable geometries is achieved by combining volumetric printing (VP) and high-resolution two-photon ablation (2PA), overcoming the challenge of generating freeform designs with features ranging from centimeter to micrometer scales. Optical tuning of the photoresin's refractive index is used to eliminate micrometer-size defects generated during VP, allowing for defect-free printing that can be combined with 2PA. A protein-based photoclick photoresin is introduced to meet the requirements of the 2PA process and facilitate VP. This hybrid strategy enables the generation of complex organotypic 3D vasculature-like constructs with multiscale features.
ADVANCED MATERIALS TECHNOLOGIES
(2023)
Article
Chemistry, Multidisciplinary
Enrico Tosoratti, Dominic Rutsche, Maryam Asadikorayem, Simone Ponta, Philipp Fisch, Killian Flegeau, Thomas Linder, Pierre Guillon, Marcy Zenobi-Wong
Summary: A novel casting technique using agarose molds was developed for fabricating complex tissue grafts. The technique allows for high-resolution, multi-layered, and human-sized tissue transplants. Different materials were used for each layer and bonded together using crosslinkers. The technique was successfully demonstrated by creating bi-layered human-sized ears.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Engineering, Biomedical
Anna Puiggali-Jou, Riccardo Rizzo, Angela Bonato, Philipp Fisch, Simone Ponta, Daniel M. Weber, Marcy Zenobi-Wong
Summary: Tissue engineering methods have potential for restoring cartilage function, but reproducing cartilage zonal architecture is still a challenge. This study demonstrates that Filamented Light biofabrication can significantly improve cartilage tissue maturity by generating constructs with zonal architecture and native-like mechanical properties.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Engineering, Biomedical
Maryam Asadikorayem, Frantisek Surman, Patrick Weber, Daniel Weber, Marcy Zenobi-Wong
Summary: A novel strategy is developed to engineer cartilage using injectable, self-healing, in situ crosslinkable zwitterionic granular hydrogels that allow for direct encapsulation of cells. The granular hydrogel is produced by mechanical fragmentation of bulk photocrosslinked hydrogels and can be enzymatically crosslinked to stabilize the construct. Encapsulated chondrocytes are viable, proliferative, and capable of producing cartilaginous extracellular matrix in the zwitterionic granular hydrogel.
ADVANCED HEALTHCARE MATERIALS
(2023)
