Article
Engineering, Biomedical
Kanika Suri, Anisha D'Souza, Di Huang, Aashray Bhavsar, Mansoor Amiji
Summary: Cancer therapy is shifting towards immunotherapy that activates the host immune system. Bacterial extracellular vesicles (BEVs) are being studied as a potential tool for controlling immune responses and for drug delivery. However, there are still unanswered questions regarding the use of BEVs, especially for long-term immunotherapies.
BIOACTIVE MATERIALS
(2023)
Review
Microbiology
Jianwei Chen, Hongfang Zhang, Siqi Wang, Yujie Du, Bin Wei, Qiang Wu, Hong Wang
Summary: Bacterial extracellular vesicles (EVs) play a significant role in bacterial infections by influencing the infection process through various mechanisms. With the increasing problem of antibiotic resistance, researching and developing novel therapeutic approaches is of high importance.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Kubra Erdogan Gover, Melis Isik, Dilan Barut, Ceyda Tuba Sengel-Turk, Gulin Amasya, Burak Derkus, Ahmet Cabuk, Pinar Aytar Celik
Summary: This study aimed to investigate the cytotoxic and anti-apoptotic effects of vesicles released from Enterococcus faecalis on colon cancer cells. The results demonstrated that these vesicles have cytotoxic and anti-proliferative effects on colon cancer cells and enhance apoptosis. This suggests that membrane vesicles have potential as nano-drug delivery systems.
BIOCHEMICAL ENGINEERING JOURNAL
(2022)
Article
Immunology
Sutonuka Bhar, Guanqi Zhao, Julia D. Bartel, Heather Sterchele, Alexa Del Mazo, Lisa E. Emerson, Mariola J. Edelmann, Melissa K. Jones
Summary: Human norovirus is a major cause of non-bacterial gastroenteritis worldwide and is the second leading cause of diarrheal deaths in children in developing countries. This study shows that bacterial extracellular vesicles may play a role in controlling murine norovirus infection through modulation of the antiviral immune response.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Microbiology
Yanzhen Wang, Xinghong Luo, Xiaozhen Xiang, Chunbo Hao, Dandan Ma
Summary: Accumulating evidence suggests that not all bidirectional microbiota-host interactions involve direct cell contact. Bacterial extracellular vesicles (BEVs) mediate microbiota functions by delivering effector molecules that modulate host signaling pathways, potentially driving or inhibiting disease pathogenesis in different systems within the host.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Thorria R. Marzoog, Majid S. Jabir, Sumayah Ibraheem, Sabrean F. Jawad, Sawsan S. Hamzah, Ghassan M. Sulaiman, Hamdoon A. Mohammed, Riaz A. Khan
Summary: Bacterial-extracellular-vesicles (BEVs) derived from Escherichia coli, strain-A5922, were used to treat colon cancer cells, HT-29. The BEVs induced oxidative stress and mitophagy in HT-29 cells, causing adenocarcinomic cytotoxicity and inhibiting cell growth. The cytotoxicity and mitophagy were mediated through the Akt/mTOR pathway, triggered by BEVs-induced cellular oxidative stress, ultimately leading to cell death.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2023)
Review
Biochemistry & Molecular Biology
Felix Behrens, Teresa C. Funk-Hilsdorf, Wolfgang M. Kuebler, Szandor Simmons
Summary: Respiratory infection-induced pneumonia is the leading cause of death in global infectious diseases, with antibiotics and vaccines helping to reduce the burden. However, lack or refusal of vaccines and antibiotics in some regions, the rise of multidrug-resistant pathogens, and high fatality rates even in patients treated appropriately pose a global threat. Bacteria's secretome contains extracellular vesicles that can trigger inflammatory responses and serve as promising vaccine candidates.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Lily A. Charpentier, Emily F. Dolben, Matthew R. Hendricks, Deborah A. Hogan, Jennifer M. Bomberger, Bruce A. Stanton
Summary: This article reviews the role of outer membrane vesicles (OMVs) in mediating the interaction between Gram-negative bacteria and their human hosts. OMVs play a critical role in facilitating host-pathogen interactions without direct cell-to-cell contact. The article describes the mechanisms of OMV formation and their interaction with host cells, including the transport of microbial protein virulence factors and sRNAs, affecting host signaling pathways. Specifically, the review highlights how OMVs can promote chronic infection through epigenetic modifications of the host immune response. The article also identifies gaps in knowledge and suggests future research directions in OMV isolation and characterization methods.
Article
Multidisciplinary Sciences
Vera Bettenworth, Simon van Vliet, Bartosz Turkowyd, Annika Bamberger, Heiko Wendt, Matthew McIntosh, Wieland Steinchen, Ulrike Endesfelder, Anke Becker
Summary: In quorum sensing, bacteria use frequency-modulated pulsing mechanism to make collective decisions. Each cell's physiological state and behavioral needs are encoded in the pulse frequency of autoinducer synthase gene expression, and the integration of pulse frequencies with other cells determines the initiation of collective behavior.
NATURE COMMUNICATIONS
(2022)
Review
Pharmacology & Pharmacy
Jin Gao, Yujie Su, Zhenjia Wang
Summary: This review provides an overview of the current research on bacterial membrane vesicles (BMVs), including their production, types, and therapeutic applications. The authors discuss various platforms of BMVs and how they can be engineered using chemical, physical, and genetic methods. The review also examines the methods of drug loading in BMVs and their limitations. Several therapeutic platforms of BMVs for improving the treatment of infectious diseases and cancers are reviewed. The authors emphasize the need for rigorous approaches and methods to develop reproducible and scalable drug delivery systems for translation.
ADVANCED DRUG DELIVERY REVIEWS
(2022)
Review
Biochemistry & Molecular Biology
Ella L. Johnston, Begona Heras, Thomas A. Kufer, Maria Kaparakis-Liaskos
Summary: BMVs produced by both Gram-negative and Gram-positive bacteria contain immune stimulating molecules that can modulate host immune response through NLR-dependent mechanisms. Commensal derived BMVs are believed to shape immunity and maintain gut homeostasis, highlighting their roles in limiting inflammatory diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Microbiology
Nikola Zlatkov, Aftab Nadeem, Bernt Eric Uhlin, Sun Nyunt Wai
Summary: Bacterial membrane vesicles (BMVs) are extracellular organelles that play a crucial role in bacterial eco-evolutionary dynamics by delivering cargo in a protected and concentrated manner. They contribute to ecosystem stability and can help delay negative effects of evolutionary trade-offs, benefiting both bacterial populations and the ecosystem as a whole.
FEMS MICROBIOLOGY REVIEWS
(2021)
Article
Nutrition & Dietetics
Natalia Diaz-Garrido, Josefa Badia, Laura Baldoma
Summary: This study aimed to understand the mechanisms of extracellular vesicles (BEVs) in driving specific immune responses. The production of cytokines and exosomes by dendritic cells (DCs) challenged with BEVs from different strains of E. coli was analyzed. The results showed that the strains had differential effects on the production of specific cytokines and the composition of immune-related miRNA in the exosomes.
Article
Biochemistry & Molecular Biology
Hongsen Liu, Tao Wei, Lin Sun, Tingting Wu, Fuqiang Li, Jianlei Zhao, Jinmeng Chu, Fei Wang, Yong Cai, Jingji Jin
Summary: The NSL HAT complex plays a role in regulating gene expression through histone acetylation, particularly affecting the transcription factor YY1, which is involved in cell proliferation, biological adhesion, and metabolic processes. MOF and NSL3 are involved in the regulation of YY1 and the downstream target gene cell division cycle 6. The NSL HAT complex may recognize specific DNA-binding sites in the promoter region of target genes for transcriptional regulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Fisheries
Md Afsar Ahmed Sumon, Mohammad Habibur Rahman Molla, Israa J. Hakeem, Foysal Ahammad, Ramzi H. Amran, Mamdoh T. Jamal, Mohamed Hosny Gabr, Md Shafiqul Islam, Md Tariqul Alam, Christopher L. Brown, Eun-Woo Lee, Mohammed Moulay, Amer H. Asseri, F. A. Dain Md Opo, Ahad Amer Alsaiari, Md Tawheed Hasan
Summary: Fish reproduction can be improved through epigenetics and probiotics, which can enhance reproductive performance and fry quality. Epigenetics involves changes in chromatin structure and function, DNA methylation, and modification of non-coding RNA molecules to transfer desired information. Probiotics induce responsiveness of follicles and change oocyte chemical composition to promote reproduction. This review provides an overview of the effects and mechanisms of epigenetics and probiotics in fish reproduction, as well as causal factors and current techniques in addressing reproductive dysfunctions.
Article
Microbiology
Henrik Seibt, Kyaw Min Aung, Takahiko Ishikawa, Annika Sjostrom, Martin Gullberg, Gemma Catherine Atkinson, Sun Nyunt Wai, Victoria Shingler
ENVIRONMENTAL MICROBIOLOGY
(2020)
Review
Microbiology
Nikola Zlatkov, Aftab Nadeem, Bernt Eric Uhlin, Sun Nyunt Wai
Summary: Bacterial membrane vesicles (BMVs) are extracellular organelles that play a crucial role in bacterial eco-evolutionary dynamics by delivering cargo in a protected and concentrated manner. They contribute to ecosystem stability and can help delay negative effects of evolutionary trade-offs, benefiting both bacterial populations and the ecosystem as a whole.
FEMS MICROBIOLOGY REVIEWS
(2021)
Article
Multidisciplinary Sciences
Thyra Loewenmark, Anna Loefgren-Burstroem, Carl Zingmark, Vincy Ekloef, Michael Dahlberg, Sun Nyunt Wai, Paer Larsson, Ingrid Ljuslinder, Sofia Edin, Richard Palmqvist
SCIENTIFIC REPORTS
(2020)
Article
Biochemistry & Molecular Biology
Bishnu Joshi, Bhupender Singh, Aftab Nadeem, Fatemeh Askarian, Sun Nyunt Wai, Mona Johannessen, Kristin Hegstad
Summary: The study revealed the presence of small RNAs associated with bacterial extracellular vesicles (EVs) released by the opportunistic pathogen Staphylococcus aureus, including potential gene regulatory small RNAs and high levels of tRNAs. Notable EV-associated small RNAs identified were SsrA, RsaC, and RNAIII, offering new insights into the role of EV-associated RNA as a modulator of host-pathogen interaction.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Oncology
Aftab Nadeem, Kyaw Min Aung, Tanusree Ray, Athar Alam, Karina Persson, Amit Pal, Bernt Eric Uhlin, Sun Nyunt Wai
Summary: Colorectal cancer is a major cause of cancer-related deaths worldwide, with APC gene mutations playing a key role in tumor development. The novel cytotoxin MakA induces apoptosis in cancer cells, with potent cytotoxic activity against cancer cell lines while sparing nontransformed cells. MakA inhibits beta-catenin-mediated tumor cell proliferation, making it a promising candidate for therapeutic development against colon cancer.
INTERNATIONAL JOURNAL OF CANCER
(2021)
Article
Microbiology
Aftab Nadeem, Athar Alam, Eric Toh, Si Lhyam Myint, Zia Ur Rehman, Tao Liu, Marta Bally, Anna Arnqvist, Hui Wang, Jun Zhu, Karina Persson, Bernt Eric Uhlin, Sun Nyunt Wai
Summary: Vibrio cholerae is the causative agent of cholera, with recent findings identifying MakA as a potent virulence factor. MakA interacts with mammalian cells through phosphatidic acid, promoting macropinocytosis and leading to autophagy activation. This discovery sheds light on the potential role of MakA as an anti-colonization factor during late stages of cholera infection.
Article
Biochemistry & Molecular Biology
Si Lhyam Myint, Nikola Zlatkov, Kyaw Min Aung, Eric Toh, Annika Sjostrom, Aftab Nadeem, Marylise Duperthuy, Bernt Eric Uhlin, Sun Nyunt Wai
Summary: This study identified two proteins in E. coli, Ecotin and LamB, that influenced E. coli susceptibility to T6SS-mediated killing by V. cholerae. Manipulating the expression of these proteins could potentially modulate inter-bacterial competition in vivo, such as in the gastrointestinal microbiome.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2021)
Article
Infectious Diseases
Nabil Karah, Sun Nyunt Wai, Bernt Eric Uhlin
Summary: The study identified different lineages of A. baumannii GC1 with distinct geographical distribution patterns, with CST2 cluster being the most prevalent subtype and another group of CSTs corresponding to the second lineage, associated with the Middle East and military healthcare facilities.
INFECTION GENETICS AND EVOLUTION
(2021)
Article
Multidisciplinary Sciences
Aftab Nadeem, Raghavendra Nagampalli, Eric Toh, Athar Alam, Si Lhyam Myint, Thomas Heidler, Mitesh Dongre, Nikola Zlatkov, Hudson Pace, Fouzia Bano, Anders Sjostedt, Marta Bally, Bernt Eric Uhlin, Sun Nyunt Wai, Karina Persson
Summary: MakA protein is part of a gene cluster in Vibrio cholerae, along with other proteins forming a tripartite cytolytic toxin that is toxic to both red blood cells and human colon carcinoma cells. This previously unrecognized toxin may contribute to fitness and virulence potential in different host environments and organisms.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biology
Aftab Nadeem, Alexandra Berg, Hudson Pace, Athar Alam, Eric Toh, Jorgen Aden, Nikola Zlatkov, Si Lhyam Myint, Karina Persson, Gerhard Grobner, Anders Sjostedt, Marta Bally, Jonas Barandun, Bernt Eric Uhlin, Sun Nyunt Wai
Summary: This study reveals a novel tubulation mechanism of an alpha-pore-forming toxin protein and a new mode of action by a secreted bacterial toxin. This protein is able to disrupt host cell membranes by forming tube-like structures under acidic conditions.
Article
Oncology
Thyra Lowenmark, Xingru Li, Anna Lofgren-Burstrom, Carl Zingmark, Agnes Ling, Therese G. Kellgren, Par Larsson, Ingrid Ljuslinder, Sun Nyunt Wai, Sofia Edin, Richard Palmqvist
Summary: This study found significant associations between Parvimonas micra and high-grade tumors as well as tumors of CMS1 subtype, and significant associations between Fusobacterium nucleatum and right-sided tumors, microsatellite instability, and CMS1 tumors. Parvimonas micra was associated with activated T lymphocytes and antigen-presenting B lymphocytes, while no associations were found between Fusobacterium nucleatum and immune profiles in this study.
CANCER IMMUNOLOGY IMMUNOTHERAPY
(2022)
Article
Immunology
Saskia F. Erttmann, Patrycja Swacha, Kyaw Min Aung, Bjorn Brindefalk, Hui Jiang, Anetta Hartova, Bernt Eric Uhlin, Sun N. Wai, Nelson O. Gekara
Summary: This study revealed the modulation of systemic immunity and response to viral infection by gut commensals. Peripheral cGAS-STING activation plays a crucial role in promoting host resistance to systemic viral infections.
Article
Biotechnology & Applied Microbiology
Dharmender K. Gahlot, Sun N. Wai, David L. Erickson, Matthew S. Francis
Summary: The study shows that the CpxA-CpxR system influences the formation of biofilms by enteropathogenic Yersinia pseudotuberculosis. Mutant bacteria with active CpxR similar to P isoform failed to form biofilms on plastic or on the surface of C. elegans nematodes. The production of exopolysaccharides, driven by the hms loci, is a major factor in biofilm formation. Active Cpx-signalling represses exopolysaccharide production, leading to a loss of biofilm formation on multiple surfaces.
NPJ BIOFILMS AND MICROBIOMES
(2022)
Article
Engineering, Biomedical
Yang Liu, Aftab Nadeem, Sujeesh Sebastian, Martin A. Olsson, Sun N. Wai, Emelie Styring, Jacob Engellau, Hanna Isaksson, Magnus Tagil, Lars Lidgren, Deepak Bushan Raina
Summary: This study demonstrates that doxorubicin (DOX) modified hydroxyapatite (HA) nanoparticles can release DOX in osteosarcoma cells, inhibiting cell migration and inducing cell apoptosis. In a mouse osteosarcoma model, locally delivered DOX via HA particles has a stronger tumor eradication effect. Additionally, it is shown that HA nanoparticles play a pivotal role in this approach, and the combination of nano and micro HA can increase the safety of particulate nanomaterials.
MATERIALS TODAY BIO
(2022)
Article
Cell Biology
Eric Toh, Palwasha Baryalai, Aftab Nadeem, Kyaw Min Aung, Sa Chen, Karina Persson, Jenny L. Persson, Bernt Eric Uhlin, Sun Nyunt Wai
Summary: The bacterial cytotoxin MakA released by Vibrio cholerae inhibits cell growth and tumor cell proliferation by targeting the PIP5K1 alpha lipid signaling pathway and inducing G2/M cell cycle arrest.
CELL DEATH & DISEASE
(2022)
