Journal
RSC ADVANCES
Volume 8, Issue 5, Pages 2365-2372Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c7ra11507e
Keywords
-
Categories
Funding
- Villum Fonden, Denmark [13153]
- Villum Fonden [00013153] Funding Source: researchfish
Ask authors/readers for more resources
Histamine is a biogenic amine naturally present in many body cells. It is also a contaminant that is mostly found in spoiled food. The consumption of foods containing high levels of histamine may lead to an allergy-like food poisoning. Analytical methods that can routinely screen histamine are thus urgently needed. In this paper, we developed a facile and cost-effective molecularly imprinted polymer (MIP)-based fluorometric assay to directly quantify histamine. Histamine-specific MIP nanoparticles (nanoMIPs) were synthesized using a modified solid-phase synthesis method. They were then immobilized in the wells of a microplate to bind the histamine in aqueous samples. After binding, o-phthaldialdehyde (OPA) was used to label the bound histamine, which converted the binding events into fluorescent signals. The obtained calibration curve of histamine showed a linear correlation ranging from 1.80 to 44.98 mM with the limit of detection of 1.80 mM. This method was successfully used to detect histamine in spiked diary milk with a recovery rate of more than 85%.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available