Article
Chemistry, Multidisciplinary
Jing Huang, Mingang Lin, Fang Zeng, Shuizhu Wu
Summary: The use of the biomarker-responsive nanoprobe DHXI@HSA enables accurate localization of deep-seated tumors and imaging-guided tumor resection surgery. This nanoprobe specifically responds to the biomarker NQO1 overexpressed in cancerous tumors, providing fluorescence and optoacoustic signals for precise tumor localization and differentiation during surgery.
MATERIALS CHEMISTRY FRONTIERS
(2021)
Article
Chemistry, Multidisciplinary
Zhuo Zeng, Junjie Chen, Lihe Sun, Fang Zeng, Shuizhu Wu
Summary: A nanoprobe has been created to detect hepatic ischemia-reperfusion injury. The nanoprobe emits noticeable optoacoustic and NIR-II fluorescent signals in response to the in situ biomarker H2O2 in the liver during ischemia-reperfusion injury.
CHEMICAL COMMUNICATIONS
(2023)
Article
Chemistry, Physical
Yixiao Li, Shaohua Qu, Yumeng Xue, Lianbing Zhang, Li Shang
Summary: A thorough understanding of antimicrobial mechanism is crucial for developing novel antibacterial agents. In this study, cationic bovine serum albumin-protected AuAgNCs were synthesized and their antimicrobial process was investigated in real-time using fluorescence imaging. The cBSA-AuAgNCs exerted their antimicrobial effects by attaching on the outer membrane of bacteria without obvious internalization. Mechanistically, the cationic NCs caused disruption to the bacterial membrane due to strong electrostatic interactions, leading to the accumulation of reactive oxygen species (ROS) and eventually bactericidal action.
Article
Chemistry, Applied
Jia-Qi Xu, Jin-Shuang Guo, Ke-Feng Xie, Meng-Jiao Gao, Ran Wei, Zhen-Hui Xin, Yan-Fei Kang
Summary: In this work, a turn-on fluorescent probe JQ-1 was designed and synthesized for detecting H2O2. The probe exhibited high selectivity, excellent sensitivity, and a fast response time. It mainly targeted mitochondria and can be used for imaging exogenous and endogenous H2O2. Furthermore, it was used to investigate the change of H2O2 level during immunogenic cell death for the first time, with negligible change observed. Overall, the probe is a reliable tool for monitoring both endogenous and exogenous H2O2.
Article
Chemistry, Analytical
Yi Qu, Zhongsheng Xu, Jiemen Wang, Wei Liu, Anam Iqbal, Kanwal Iqbal, Yaling Su, Yuping Cao, Jilu Yang, Wenwu Qin, Yun Liu
Summary: A novel fluorescent probe NBD-3 was developed to detect endogenous chymotrypsin. Through computation and spectral testing, the best probe NBD-3 with rare bright red fluorescence was screened. The presence of endogenous chymotrypsin was discovered for the first time in the liver of mice through confocal imaging, in vivo imaging, and in vitro imaging of organs. NBD-3's strong performance allows real-time detection of chymotrypsin medications.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Multidisciplinary Sciences
Yinglong Wu, Lihe Sun, Xiaokai Chen, Jiawei Liu, Juan Ouyang, Xiaodong Zhang, Yi Guo, Yun Chen, Wei Yuan, Dongdong Wang, Ting He, Fang Zeng, Hongzhong Chen, Shuizhu Wu, Yanli Zhao
Summary: The authors address the challenges in using organic small-molecule contrast agents for multispectral optoacoustic imaging by constructing water-dispersible supramolecular optoacoustic assemblies based on cucurbit[8]uril. The constructed assemblies show enhanced optoacoustic performance due to increased absorption and decreased fluorescence. The formulated assemblies can effectively detect and diagnose various tumors and kidney injury in mouse models using multispectral optoacoustic imaging.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Applied
Yin Ai, Haichang Ding, Congbin Fan, Gang Liu, Shouzhi Pu
Summary: In this study, a fluorescent probe named DTRN was synthesized for the detection and imaging of Cysteine and Homocysteine in living systems. DTRN exhibited excellent sensitivity, specificity, and fast response, and it was also capable of detecting Cysteine in HeLa cells.
Review
Biochemistry & Molecular Biology
Adrian P. Regensburger, Emma Brown, Gerhard Kronke, Maximilian J. Waldner, Ferdinand Knieling
Summary: Optoacoustic or photoacoustic imaging technology allows non-invasive visualization of laser-illuminated tissue with high resolution and penetration depth, making it a versatile tool for biomedical research. When it comes to imaging inflammation, this technology offers advantages over current clinical methods in diagnosing and monitoring physiological and pathophysiological processes.
Review
Biotechnology & Applied Microbiology
Xue-feng Shi, Bin Ji, Yanyan Kong, Yihui Guan, Ruiqing Ni
Summary: Optoacoustic imaging has versatile applications in biomedical research, visualizing disease pathophysiology and monitoring treatment effects in animal models, as well as in clinical settings. Multimodal imaging provides insights into the complex disease mechanisms with different molecular, structural, and functional readouts in vivo. Rapid development in molecular imaging contrast agents employing a multimodal imaging strategy for pathological targets involved in brain diseases is ongoing.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Jatinder Kaur, Atul Bhardwaj, Frank Wuest
Summary: The study introduces a new chemical toolbox for precise visualization and tracking of COX-2 in live cells with superior sensitivity in the visible range. By in cellulo assembly of fluorescent probes, COX-2 imaging was successfully achieved and protein-ligand interactions were investigated.
CHEMISTRY-A EUROPEAN JOURNAL
(2021)
Review
Chemistry, Inorganic & Nuclear
Juan Ouyang, Lihe Sun, Fang Zeng, Shuizhu Wu
Summary: Fluorophores with aggregation-induced emission (AIE) have many advantageous features for biomedical applications, especially for early diagnosis of diseases. Probes play a crucial role in fluorescent and optoacoustic imaging, and biomarker-activatable probes based on smart AIEgens have been widely researched and applied in this field.
COORDINATION CHEMISTRY REVIEWS
(2022)
Article
Chemistry, Inorganic & Nuclear
Camille Gosee, Juliette Moreau, Cyril Cadiou, Maite Callewaert, Celine Henoumont, Lionel Larbanoix, Michael Molinari, Sorina N. Voicu, Christophe Portefaix, Sophie Laurent, Francoise Chuburu
Summary: The challenge of imaging is to combine resolution and sensitivity in order to gain accuracy in diagnosis. No single modality can provide comprehensive information. Then, the solution is to design probes that are able to gather on a single platform the best features of the different imaging modalities. To achieve this objective, we have combined two types of probes, one associated with photoacoustic imaging (PAI) and the other with magnetic resonance imaging (MRI), within polysaccharide-based nanohydrogels.
INORGANIC CHEMISTRY FRONTIERS
(2023)
Article
Chemistry, Multidisciplinary
Zunpan She, Junjie Chen, Lihe Sun, Fang Zeng, Shuizhu Wu
Summary: An NO-responsive probe has been developed for imaging acute inflammation, which exhibits turn-on NIR-II fluorescence and optoacoustic signals in situ NO at acute inflammation sites such as LPS-induced acute dermatitis and MIA-induced acute joint inflammation.
CHEMICAL COMMUNICATIONS
(2022)
Article
Chemistry, Inorganic & Nuclear
Camille Gosee, Juliette Moreau, Cyril Cadiou, Maite Callewaert, Celine Henoumont, Lionel Larbanoix, Michael Molinari, Sorina N. Voicu, Christophe Portefaix, Sophie Laurent, Francoise Chuburu
Summary: The challenge in imaging is to achieve accurate diagnosis by combining resolution and sensitivity. To overcome the limitations of single modality imaging, a solution is to design probes that combine the best features of different imaging modalities. In this study, we successfully combined photoacoustic imaging and magnetic resonance imaging within polysaccharide-based nanohydrogels to create an 'all in one' bimodal imaging system. The nanohydrogels showed significant signals in both MSOT and MRI experiments, demonstrating their potential for accurate imaging.
INORGANIC CHEMISTRY FRONTIERS
(2023)
Article
Neurosciences
Marta Ramos-Vega, Pontus Kjellman, Mihail Ivilinov Todorov, Tekla Maria Kylkilahti, B. Thomas Backstrom, Ali Erturk, Chris Denis Madsen, Iben Lundgaard
Summary: Recent studies have revealed that metabolic changes and oxygen deficiency in the central nervous system are important factors in the development of multiple sclerosis (MS). In this study, researchers investigated the changes in oxygenation and blood flow in the spinal cord of a mouse model of MS. They used multispectral optoacoustic tomography to analyze the spinal cord before and after oxygen administration in mice with experimental autoimmune encephalomyelitis (EAE), a model for MS. Additionally, they utilized a novel deep learning-based framework called VesSAP to analyze the vascular structure of the intact spine. The study found that EAE mice had lower oxygen saturation and hemoglobin concentration in the spinal cord, indicating compromised blood flow. However, oxygen administration improved the hypoxic condition in the spinal cord, although the ventral region remained hypoxic. Despite increased vascular density, the researchers observed a reduction in the length and complexity of the perfused vascular network in EAE. Overall, these findings highlight the importance of considering hypoxia and spinal vascular perfusion in the neuroinflammatory pathology of EAE and MS. The study also introduces optoacoustic imaging as a potential technique for further understanding the role of hypoxia in EAE and monitoring it in MS patients.
ACTA NEUROPATHOLOGICA COMMUNICATIONS
(2022)
Article
Computer Science, Interdisciplinary Applications
Justine Robin, Ali Ozbek, Michael Reiss, Xose Luis Dean-Ben, Daniel Razansky
Summary: Spherical matrix arrays, combined with clinically-approved microbubble injection, enable multimodal imaging for non-invasive mapping of vascular networks and oxygenation in deep tissues. In experiments, this method achieved high spatial resolution and signal to noise ratio, while allowing for flow velocity mapping.
IEEE TRANSACTIONS ON MEDICAL IMAGING
(2022)
Article
Physics, Applied
Urs A. T. Hofmann, Sergio Perez-Lopez, Hector Estrada, Daniel Razansky
Summary: HOPE ultrasound leverages high-order reflections to improve traditional ultrasound imaging, demonstrating the ability to resolve submicrometer features, enhance contrast, and improve accuracy without additional hardware, making it easy to implement.
PHYSICAL REVIEW APPLIED
(2022)
Article
Oncology
Anna Orlova, Ksenia Pavlova, Aleksey Kurnikov, Anna Maslennikova, Marina Myagcheva, Evgeniy Zakharov, Dmitry Skamnitskiy, Valeria Perekatova, Alexander Khilov, Andrey Kovalchuk, Alexander Moiseev, Ilya Turchin, Daniel Razansky, Pavel Subochev
Summary: This study demonstrates the size-dependent nature of vascular responses to radiation treatments at varying doses in vivo using scanning optoacoustic (OA) microangiography. The OA approach enables the study of radiation-induced vascular changes in an unperturbed in vivo environment while allowing for high-resolution observations of deep tissue at the whole tumor scale.
Article
Multidisciplinary Sciences
Paul Wrede, Oleksiy Degtyaruk, Sandeep Kumar Kalva, Xose Luis Dean-Ben, Ugur Bozuyuk, Amirreza Aghakhani, Birgul Akolpoglu, Metin Sitti, Daniel Razansky
Summary: This study proposed a method to detect and track circulating microrobots in real-time using optoacoustic imaging, by designing cell-sized nickel-based spherical Janus magnetic microrobots and enhancing their near-infrared optoacoustic signature through gold conjugation. The microrobots of different diameters were successfully detected in both laboratory and blood-containing environments, demonstrating the ability to track and manipulate them in real-time in murine cerebral vasculature.
Article
Chemistry, Multidisciplinary
Zhenyue Chen, Quanyu Zhou, Xose Luis Dean-Ben, Irmak Gezginer, Ruiqing Ni, Michael Reiss, Shy Shoham, Daniel Razansky
Summary: Modern optical neuroimaging approaches have expanded our ability to study complex brain functions by developing a hybrid imaging platform that allows simultaneous observation of calcium and hemodynamic activation patterns. This research provides valuable insights into neurovascular and neurometabolic coupling mechanisms and related diseases.
Article
Optics
Ruiqing Ni, Xose Luis Dean-Ben, Valerie Treyer, Anton Gietl, Christoph Hock, Jan Klohs, Roger M. Nitsch, Daniel Razansky
Summary: Imaging modalities, such as multi-spectral optoacoustic tomography (MSOT), have greatly advanced neurology and brain research. Non-invasive transcranial MSOT angiography has been demonstrated to visualize pial veins in the human brain, which can be beneficial for clinical brain imaging.
Article
Biochemical Research Methods
Wuwei Ren, Xose Luis Dean-ben, Zhiva Skachokova, Mark-Aurel Augath, Ruiqing Ni, Zhenyue Chen, Daniel Razansky
Summary: Progress in brain research relies on next-generation multi-modal imaging tools capable of capturing transient functional events and multiplexed contrasts noninvasively. This study presents a hybrid magnetic resonance and optoacoustic tomography system for murine brain imaging, enabling simultaneous dual-modality visualization of contrast agent dynamics. The system combines MR-compatible spherical matrix array transducer, fiber-based light illumination, and an optimized radiofrequency coil for whole-brain interrogation.
BIOMEDICAL OPTICS EXPRESS
(2023)
Article
Chemistry, Multidisciplinary
Quanyu Zhou, Daniil Nozdriukhin, Zhenyue Chen, Lukas Glandorf, Urs A. T. Hofmann, Michael Reiss, Lin Tang, Xose Luis Dean-Ben, Daniel Razansky
Summary: This study introduces a new method for volumetric deep-tissue microangiography that overcomes the limitations of light diffusion and optical diffraction in wide-field imaging configurations. The proposed method combines stereovision and super-resolution localization imaging to achieve high-resolution 3D imaging and accurate blood flow quantification, enabling detailed visualization and analysis of microvascular networks.
Article
Chemistry, Multidisciplinary
Zhenyue Chen, Irmak Gezginer, Mark-Aurel Augath, Yu-Hang Liu, Ruiqing Ni, Xose Luis Dean-Ben, Daniel Razansky
Summary: This article reports on the development of a fully hybridized system enabling concurrent functional magnetic resonance optoacoustic tomography (MROT) measurements of stimulus-evoked brain-wide sensory responses in mice. The results indicate that the BOLD signals are highly correlated, both spatially and temporally, with the total hemoglobin readings resolved with volumetric multi-spectral optoacoustic tomography. Furthermore, the differential oxygenated and deoxygenated hemoglobin optoacoustic readings exhibit superior sensitivity as compared to the BOLD signals when detecting stimulus-evoked hemodynamic responses.
Article
Biochemical Research Methods
Sandeep Kumar Kalva, Xose Luis Dean-Ben, Michael Reiss, Daniel Razansky
Summary: The authors propose spiral volumetric optoacoustic tomography (SVOT) for visualizing contrast agent perfusion and biodistribution in mice. The method overcomes the limitations of current whole-body imaging by providing optical contrast and unprecedented spatial and temporal resolution. SVOT allows visualization of deep-seated structures in living tissues and real-time imaging of biodynamics at the whole-organ level.
Article
Multidisciplinary Sciences
Xose Luis Dean-Ben, Justine Robin, Daniil Nozdriukhin, Ruiqing Ni, Jim Zhao, Chaim Gluck, Jeanne Droux, Juan Sendon-Lago, Zhenyue Chen, Quanyu Zhou, Bruno Weber, Susanne Wegener, Anxo Vidal, Michael Arand, Mohamad El Amki, Daniel Razansky
Summary: By using dichloromethane microdroplets with high optical absorption, the authors demonstrate 3D microangiography of the mouse brain, overcoming the issue of strong background absorption from red blood cells.
NATURE COMMUNICATIONS
(2023)
Proceedings Paper
Acoustics
Sandeep Kumar Kalva, Xose Luis Dean-Ben, Michael Reiss, Daniel Razansky
Summary: sSVOT is a novel approach for whole-body three-dimensional imaging, which can be completed in a short period of time. It utilizes a spherical matrix array transducer and a multibeam illumination approach to achieve high imaging speed and large field of view. sSVOT enables the visualization of intricate vascular and organ anatomy, opening new avenues for studying large-scale biological dynamics.
PHOTONS PLUS ULTRASOUND: IMAGING AND SENSING 2022
(2022)
Proceedings Paper
Acoustics
Xose Luis Dean-Ben, Daniel Razansky
Summary: This study investigates the preservation of acoustic distortion induced by the skull when optoacoustic waves are generated at neighboring point sources, introducing the concept of the "optoacoustic memory effect" for building a three-dimensional optoacoustic model. Model-based inversion using this approximation accurately recovers absorption distribution with comparable spatial resolution to that obtained without the presence of the skull.
PHOTONS PLUS ULTRASOUND: IMAGING AND SENSING 2022
(2022)
