Article
Engineering, Environmental
Julian D. Munoz Sierra, Margreet J. Oosterkamp, Henri Spanjers, Jules B. van Lier
Summary: This study investigates the effects of high and fluctuating salinity on the performance of an anaerobic membrane bioreactor (AnMBR) in treating phenol-containing wastewater. The findings show that phenol and COD removal efficiencies are affected by salinity levels, with methanogenesis being more severely impacted. Changes in salinity also significantly reduce microbial richness and diversity, while the AnMBR remains effective for treating chemical process water characterized by high phenols concentrations and fluctuating salinity levels.
CHEMICAL ENGINEERING JOURNAL
(2021)
Article
Agricultural Engineering
Libing Zheng, Chun Zhang, Rui Gao, Liangchang Zhang, Weidang Ai, Mathias Ulbricht, Yuansong Wei
Summary: This work focuses on the treatment of hygiene wastewater using anaerobic membrane bioreactor (AnMBR), specifically surfactant degradation and microbial community succession. By implementing a urine source-separation strategy, the removal efficiency of COD and surfactants reached 90%-97% and 80% respectively. The microbial community gradually shifted from methane-producing microorganisms to sulfur-metabolizing and surfactant-degrading bacteria. This study provides insights into the degradation mechanism and presents an effective solution for hygiene wastewater treatment, supporting the future development of AnMBR technology.
BIORESOURCE TECHNOLOGY
(2023)
Article
Engineering, Environmental
Victor S. Garcia Rea, Beatriz Egerland Bueno, Daniel Cerqueda-Garcia, Julian D. Munoz Sierra, Henri Spanjers, Jules B. van Lier
Summary: The study demonstrated the simultaneous degradation of phenol, p-cresol, and resorcinol under anaerobic saline conditions. Using anaerobic phenol-degrading biomass, it was found that the degradation rates of p-cresol and resorcinol were feasible. This research provides new perspectives for high-rate anaerobic treatment of chemical wastewater.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Environmental Sciences
Kanming Wang, Haoliang Zhang, Yuxiang Shen, Jiale Li, Wu Zhou, Hualong Song, Min Liu, Hongyu Wang
Summary: This study evaluated the impact of salinity on the treatment performance of an upflow anaerobic sludge blanket (UASB) configured AnMBR. Results showed that increased salinity jeopardized the COD removal efficiency of AnMBR, but had a marginal effect on dye removal efficacy. Low salinity boosted biogas production, while high salinity had a negative impact. Additionally, the increase of salinity resulted in the concentration of soluble microbial production (SMP) soar and membrane fouling rate increase, peaking at a salinity of 10 g/L.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2023)
Article
Environmental Sciences
Zhiye Sun, Yi Li, Ming Li, Ning Wang, Jun Liu, Hong Guo, Baoan Li
Summary: A bench-scale two-stage membrane-aerated biofilm reactor system was successfully applied to treat steel pickling rinse wastewater with high salinity and refractory organic. The system achieved shortcut nitrification at low aeration and was not significantly affected by salinity below 4%. The key role of proteins in responding to environmental changes and the prominent salt-tolerance of EPS in the biofilm were demonstrated. The dominant salt-tolerant bacteria were identified as nitrifiers, denitrifiers, and aerobic denitrifiers.
Article
Agricultural Engineering
Di Luo, Jie Qian, Xing Jin, Li Zhang, Kun You, Peng-fei Yu, Jin-xiang Fu
Summary: Phenol has a minimal inhibitory effect on anammox process and even activates denitrification, increasing the nitrogen removal rate. High concentration of phenol significantly inhibits anammox and reduces the extracellular polymeric substance of granular sludge. Phenol promotes the proliferation of phenol-degrading denitrifying bacteria and prevents the first step of the anammox reaction.
BIORESOURCE TECHNOLOGY
(2022)
Article
Engineering, Environmental
Huaqiang Chu, Xiaoqian Liu, Jiaying Ma, Tian Li, Haifeng Fan, Xuefei Zhou, Yalei Zhang, Enchao Li, Xiwang Zhang
Summary: A full-scale coking wastewater treatment plant with a two-stage anoxic-oxic-anoxic-oxic (A1/O1/A2/O2) process was systematically investigated, showing excellent removal capacity for COD, ammonia, nitrate, and total nitrogen. The microbial community analysis revealed significant changes in bacterial communities during the AOAO process, with shifts in functional microorganisms responsible for organics and nitrogen removal. Metagenomic sequencing also indicated variations in amino acid and carbohydrate metabolism between the primary and secondary AO processes.
CHEMICAL ENGINEERING JOURNAL
(2021)
Article
Engineering, Environmental
Marie Riisgaard-Jensen, Giulia Dottorini, Marta Nierychlo, Per Halkjaer Nielsen
Summary: The immigration of bacteria in influent wastewater has a strong impact on the microbial community of activated sludge in wastewater treatment plants. The use of primary settlers can selectively increase or reduce the abundance of certain bacteria. The process of primary settling changes the microbial community of influent wastewater and can be a candidate for upstream control to optimize the assembly of microbial communities in activated sludge.
Article
Agricultural Engineering
Kaili Ma, Zhiguo Cao, Yanrui Cui, Tingting Chen, Shijie Shan, Yuyang Shi, Wei Wang, Jinghua Lv
Summary: The addition of magnetite at different salinity levels enhances methane production in anaerobic digestion, increases granule formation, and promotes partnerships between hydrogenotrophic methanogens and syntrophic bacteria.
BIORESOURCE TECHNOLOGY
(2021)
Article
Engineering, Environmental
Jing Zhu, Hong You, Zhipeng Li, Binghan Xie, Hongying Chen, Yi Ding, Shaojie Qi, Weirun Li, Binyu Ma, Xiankun Qu, Haili Tan, Feng Liu, How Yong Ng
Summary: The study investigated the application of photogranules in high saline mariculture wastewater treatment. The results showed that the internal circulating photogranular membrane bioreactor (IC-PMBR) exhibited better pollutant removal performance than the internal circulating photogranular sequencing batch bioreactor (IC-PSBR). The study also identified the key players in photogranules and demonstrated the role of different microorganisms in nitrogen and phosphate removal.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Engineering, Environmental
Hui Chen, Yangyang Wang, Zhu Chen, Zhiqiang Wu, Xueyan Chu, Shengqiang Qing, Longqi Xu, Kang Yang, Qingyuanhao Meng, Haina Cheng, Wenhao Zhan, Yuguang Wang, Hongbo Zhou
Summary: Salinity is a major concern in the biological treatment of high-salinity wastewater. Understanding how microorganisms respond to salinity stress and their impact on ecosystem function and community stability is crucial. However, little is known about the relationships between microorganisms under salinity stress. Therefore, a study was conducted using an anoxic-oxic reactor to investigate the effects of elevated salinity on system performance, microbial community dynamics, and co-occurrence networks during urine wastewater treatment. The results showed that the reactor achieved high removal rates of urea and COD across a wide range of salinity levels. Increased salinity resulted in decreased microbial alpha diversity and significant changes in beta diversity. The co-occurrence networks showed a greater proportion of negative correlations under elevated salinity, suggesting a survival strategy for microorganisms. Clostridiales and Oceanospirillales played important roles in maintaining system stability, with the oxic communities being more stable than the anoxic communities under salinity stress. These findings provide new insights into microbial relationships in response to salinity and offer a reference basis for the efficient treatment of high-salinity wastewater.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Biotechnology & Applied Microbiology
Syeed Md Iskander, Yamrot M. Amha, Phillip Wang, Qin Dong, Juhe Liu, Michael Corbett, Adam L. Smith
Summary: Co-digestion of fats, oils, and grease (FOG) with food waste (FW) can improve energy recovery in anaerobic membrane bioreactors (AnMBRs), especially by introducing more biogas production through a two-phase (TP-MP) reactor. Co-digestion of FW with FOG can also significantly increase daily biogas production and have a positive impact on microbial communities.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Engineering, Environmental
Victor S. Garcia Rea, Julian D. Munoz Sierra, Amer S. El-Kalliny, Daniel Cerqueda-Garcia, Ralph E. F. Lindeboom, Henri Spanjers, Jules B. van Lier
Summary: The conversion of phenol under saline thermophilic anaerobic conditions requires a specialized microbial community. A research conducted using an anaerobic membrane bioreactor showed that phenol degradation was limited when it was the sole substrate, but significantly increased when acetate was provided simultaneously. Isotopic analysis revealed that acetate was first oxidized to hydrogen and CO2, leading to an increased abundance of hydrogenotrophic methanogens, which played a key role in enhancing thermophilic phenol degradation.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Engineering, Environmental
Huimin Wang, Xiaoyan Qi, Siyu Chen, Xia Wang
Summary: In this study, breeding wastewater was efficiently treated using an electroactive microbial community screened from the native microorganisms of the wastewater in microbial fuel cells. The results showed good power generation and high pollutants removal efficiency. The morphology characterization demonstrated the formation of a thick biofilm by the screened electroactive microbial community. Microbial community analysis revealed an increase in microorganisms associated with electricity generation and pollutant degradation, while pathogenic microorganisms were greatly decreased. These findings suggest that the MFC constructed using the screened electroactive microbial community can be a sustainable method for breeding wastewater treatment.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2022)
Article
Microbiology
Xiaonan Liu, Yong Nie, Xiao-Lei Wu
Summary: In this study, artificial neural networks were used to predict the microbial compositions of activated sludge systems in wastewater treatment plants worldwide, achieving a prediction accuracy of 60.42%. The predictability of the microbial community was found to be significantly correlated with relative abundance and occurrence frequency, but negatively correlated with potential migration rate. The study also successfully predicted the major functional groups in the activated sludge systems using the neural network models. The prediction of microbial community in activated sludge systems provides insights for improving operating parameters and controlling community structure.
Article
Engineering, Environmental
Julian D. Munoz Sierra, Margreet J. Oosterkamp, Henri Spanjers, Jules B. van Lier
Summary: This study investigates the effects of high and fluctuating salinity on the performance of an anaerobic membrane bioreactor (AnMBR) in treating phenol-containing wastewater. The findings show that phenol and COD removal efficiencies are affected by salinity levels, with methanogenesis being more severely impacted. Changes in salinity also significantly reduce microbial richness and diversity, while the AnMBR remains effective for treating chemical process water characterized by high phenols concentrations and fluctuating salinity levels.
CHEMICAL ENGINEERING JOURNAL
(2021)
Article
Engineering, Environmental
Niels van Linden, Henri Spanjers, Jules B. van Lier
Summary: Gaseous ammonia recovered from water can be used in solid oxide fuel cells for electricity generation. Increasing feed water temperature and NH3 concentration leads to higher NH3 concentrations in gaseous membrane stripping permeate. Additionally, combining VMS and SOFC-O can result in electricity generation with higher efficiency than VMS energy consumption.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Engineering, Environmental
Victor S. Garcia Rea, Beatriz Egerland Bueno, Daniel Cerqueda-Garcia, Julian D. Munoz Sierra, Henri Spanjers, Jules B. van Lier
Summary: The study demonstrated the simultaneous degradation of phenol, p-cresol, and resorcinol under anaerobic saline conditions. Using anaerobic phenol-degrading biomass, it was found that the degradation rates of p-cresol and resorcinol were feasible. This research provides new perspectives for high-rate anaerobic treatment of chemical wastewater.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Engineering, Chemical
Niels van Linden, Yundan Wang, Ernst Sudholter, Henri Spanjers, Jules B. van Lier
Summary: The study assessed the selectivity of NH3 over H2O transfer for different types of membranes, finding that the PV membranes did not show selectivity in NH3 transfer. Compared to porous PTFE membranes, dense silica-based PV membranes did not allow for the recovery of gaseous NH3 with lower H2O content in the recovered gas.
JOURNAL OF MEMBRANE SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Magela Odriozola, Jules B. van Lier, Henri Spanjers
Summary: This research aims to develop an optimized control strategy for flux enhancer dosing in an AnMBR by creating a comprehensive integrated mathematical model. The results suggest that a feedback sludge filterability controller, which continuously doses flux enhancers, is the most appropriate control tool.
Editorial Material
Environmental Sciences
Yongjun Zhang, Jiangyong Hu, Philiswa Nosizo Nomngongo, Qilin Wang, Henri Spanjers
FRONTIERS IN ENVIRONMENTAL SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Zhe Deng, Ana Lucia Morgado Ferreira, Henri Spanjers, Jules B. van Lier
Summary: Protein-rich agro-industrial waste streams are a major environmental problem. Anaerobic digestion is commonly used to treat these waste streams, but the presence of carbohydrates can slow down protein degradation. This study investigated the mechanism behind this retardation and found that the presence of lactose shifted the microbial community and reduced protein degradation. The microorganisms in the community preferred to consume carbohydrates before proteins, leading to decreased protease and deamination activity.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Engineering, Environmental
Victor S. Garcia Rea, Julian D. Munoz Sierra, Amer S. El-Kalliny, Daniel Cerqueda-Garcia, Ralph E. F. Lindeboom, Henri Spanjers, Jules B. van Lier
Summary: The conversion of phenol under saline thermophilic anaerobic conditions requires a specialized microbial community. A research conducted using an anaerobic membrane bioreactor showed that phenol degradation was limited when it was the sole substrate, but significantly increased when acetate was provided simultaneously. Isotopic analysis revealed that acetate was first oxidized to hydrogen and CO2, leading to an increased abundance of hydrogenotrophic methanogens, which played a key role in enhancing thermophilic phenol degradation.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Green & Sustainable Science & Technology
Yu Chen, Nuttapon Toosumran, Noura Chehab, Henri Spanjers, Oguzhan Copurogu
Summary: This study proposes the use of desalination brine as a setting and hydration activator in 3D concrete printing and conducts a series of tests. The results show that adding desalination brine reduces the flowability and initial setting time of the concrete, enhances buildability, stimulates stiffness development and hydration process, thus improving the strength of the concrete.
JOURNAL OF CLEANER PRODUCTION
(2022)
Article
Water Resources
Marc Arpad Boncz, Niels van Linden, Amir Haidari, Yundan Wang, Henri Spanjers
Summary: Traditionally, industrial processes generate wastes that are often discarded, leading to environmental pollution and resource depletion. This study proposes a process for the recovery of valuable materials from waste brines, specifically magnesium and calcium salts, through nanofiltration and chemical precipitation. The results from simulations indicate that high recovery rates can be achieved, with calcium salts having higher purity than magnesium salts.
WATER RESOURCES AND INDUSTRY
(2022)
Article
Engineering, Environmental
Victor S. Garcia Rea, Beatriz Egerland Bueno, Julian D. Munoz Sierra, Athira Nair, Israel J. Lopez Prieto, Daniel Cerqueda-Garcia, Jules B. van Lier, Henri Spanjers
Summary: In this study, the chemical characteristics of bitumen fume condensate (BFC) were analyzed, and its degradation under anaerobic conditions was evaluated. The results showed that BFC contains a wide range of potentially toxic organic pollutants that negatively affect anaerobic processes. Methanogenic biomass exhibited high adaptability to the constituents of BFC. Additionally, an AnMBR reactor was successfully operated for the treatment of BFC, achieving a maximum COD removal efficiency of 87.7%.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Engineering, Environmental
Peng Wei, Wim Uijttewaal, Henri Spanjers, Jules B. van Lier, Merle de Kreuk
Summary: This study used computational fluid dynamics (CFD) to assess the impact of treated sludge rheology on flow and mixing characterisation in a full-scale biogas-mixed digester. The results showed that the applied gas-sparging induced insufficient flow and mixing, but contributed to the well-functioning of the digester. To correctly assess flow and mixing, the applied rheological data should be in agreement with the type of sludge that is treated in the digester.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Engineering, Environmental
Agha Zeeshan Ali, Sadhna Jagannathan, Yasmina-Doekhi Bennani, Jan Peter van der Hoek, Henri Spanjers
Summary: This research studied the application of photoelectrocatalytic (PEC) based advanced oxidation process (AOP) for the removal of multiple organic micro-pollutants (OMPs). It validated the simultaneous removal of acetaminophen (ACT), benzotriazole (BTA), and propranolol (PRO) using BiVO4 photoanodes under solar simulated light.
JOURNAL OF WATER PROCESS ENGINEERING
(2023)
Article
Engineering, Environmental
Rong-Gui Zhu, Chang-Gui Pan, Feng-Jiao Peng, Chao-Yang Zhou, Jun-Jie Hu, Kefu Yu
Summary: This comprehensive survey investigated the occurrence, bioaccumulation, and trophic magnification of parabens and their metabolite 4-HB in a marine food web. Results showed that parabens were the predominant pollutants in marine organisms, with significant bioaccumulation from sediments. The estimated trophic magnification factor indicated biomagnification for MeP and trophic dilution for 4-HB. Overall, the risks for humans consuming marine organisms were found to be low.
Article
Engineering, Environmental
Andres F. Torres-Franco, Deborah Leroy-Freitas, Cristina Martinez-Fraile, Elisa Rodriguez, Pedro A. Garcia-Encina, Raul Munoz
Summary: Anaerobic and microalgae-based technologies have emerged as sustainable alternatives for municipal wastewater treatment. However, the presence of viruses in the treated wastewater is a major concern for reuse applications. This study assessed the ability of these technologies to reduce viruses during secondary wastewater treatment. The results showed that all technologies were effective in reducing the concentration of viruses, with microalgae-based treatment exhibiting the highest potential for reducing the disinfection requirements of treated wastewater.
Article
Engineering, Environmental
Young Gwang Kim, Sae Yun Kwon, Spencer J. Washburn, Scott C. Brooks, Ji Won Yoon, Lucien Besnard
Summary: The study uses Hg isotope ratios to identify the sources and exposure pathways of mercury in bivalves, finding that dissolved Hg phases in the water column are the primary source and exposure pathway to bivalves. This provides new insights into using bivalves as bioindicators for sediment quality monitoring.
Article
Engineering, Environmental
Hui Geng, Ying Xu, Rui Liu, Dianhai Yang, Xiaohu Dai
Summary: This study investigates the effect of cation exchange resin (CER) on the sequential recovery of hydrogen and methane from anaerobic digestion (AD) and the corresponding mechanisms. The results show that CER can simultaneously enhance the production of hydrogen and methane by promoting the solubilisation, hydrolysis, and acidification of organic matter. Additionally, CER facilitates effective contact between bacteria and organic particulates and reduces the energy barrier for mass transfer during methane production. The study also reveals changes in the microbial community structure and metagenomics during the AD process.
Article
Engineering, Environmental
Xiaojing Lin, Zhan Jin, Shunfeng Jiang, Zhiquan Wang, Suqing Wu, Ke Bei, Min Zhao, Xiangyong Zheng
Summary: Dehumidification combined with addition of absorbent resin supplement (ARS) was used to achieve rapid evaporation of non-pretreated urine, resulting in high water evaporation efficiency and nutrient recovery.
Article
Engineering, Environmental
Yangli Che, Chaoran Lin, Shen Li, Jiao Liu, Longhai Zhu, Shilei Yu, Nan Wang, Haoshuai Li, Mutai Bao, Yang Zhou, Tonghao Si, Rui Bao
Summary: Hydrodynamic processes play a crucial role in the transmission of sediments, microbial assembly, and organic carbon redistribution in the ocean. Through experiments and analysis, we found that hydrodynamics shape the assembly of microbial communities and control the redistribution of different sourced organic carbon, thereby influencing microbial-mediated biogeochemical transformation.
Article
Engineering, Environmental
Chao Chen, Yu Yang, Nigel J. D. Graham, Zhenyu Li, Xingtao Yang, Zhining Wang, Nadia Farhat, Johannes S. Vrouwenvelder, Li -an Hou
Summary: The fouling of seawater reverse osmosis membranes is a persistent challenge in desalination. This study monitored the operational performance of a desalination plant for 7 years and the fouling development in different areas of membrane modules. The findings showed that operational performance declined over time and fouling mainly occurred at the feed side of the modules, with the highest microbial diversity. Keystone species like Chloroflexi and Planctomycetes played an important role in maintaining community structure and biofilm maturation. Polysaccharides, soluble microbial products, marine humic acid-like substances, and inorganic substances contributed to fouling. Overall, biofouling had a significant impact on membrane fouling after 7 years of operation.
Article
Engineering, Environmental
Dan Li, Jieyi Sun, Yibo Fu, Wentao Hong, Heli Wang, Qian Yang, Junhong Wu, Sen Yang, Jianhui Xu, Yunfei Zhang, Yirong Deng, Yin Zhong, Ping'an Peng
Summary: Sulfidation-oxidation treatment of magnetite (Fe3O4) enhances the production of dark center dot OH, which can efficiently degrade dissolved organic matter (DOM) and accelerate carbon cycling.
Article
Engineering, Environmental
Cheng Yu, Kaijun Wang, Kaiyuan Zhang, Ruiyang Liu, Pingping Zheng
Summary: This study implemented a microaerobic-aerobic configuration in a full-scale municipal wastewater treatment facility and investigated the effects on sludge characteristics, pollutant removal, microbial community, and granulation mechanisms. The results showed successful transition from flocculent-activated sludge to well-defined AGS after two months of operation. The primary pathways for pollutant removal were simultaneous nitrification, denitrification, and phosphorus removal. Moreover, the incorporation of internal separators induced shifts in the flow pattern, which promoted granulation.
Article
Engineering, Environmental
Zhe Zhang, Shaoyang Hu, Guangrong Sun, Wei Wang
Summary: Halogenated aromatic disinfection byproducts (DBPs), such as halogenated phenols, have garnered widespread attention due to their high toxicity and prevalence. This study reports on the analysis, occurrence, and cytotoxicity of a group of emerging halogenated aromatic DBPs, known as halogenated polyhydroxyphenols (HPPs), in drinking water.
Article
Engineering, Environmental
Shengyue Chen, Jinliang Huang, Peng Wang, Xi Tang, Zhenyu Zhang
Summary: Accurate prediction of river water quality is crucial for sustainable water management. This study introduces wavelet analysis and transfer learning techniques to assist LSTM modeling, proposing a newly coupled modeling approach that improves short-term prediction of river water quality.
Article
Engineering, Environmental
Bang Du, Xinmin Zhan, Piet N. L. Lens, Yifeng Zhang, Guangxue Wu
Summary: Efficient anaerobic digestion relies on the cooperation of different microorganisms with different metabolic pathways. This study investigated the effects of different operational modes and the addition of powdered activated carbon (PAC) on ethanol metabolic pathways. The results showed that the SBR mode and the presence of CO2 facilitated ethanol metabolism towards propionate production, while the CFR mode with extended solids retention time enriched Geobacter. Adjusting operational modes and PAC addition can modulate anaerobic ethanol metabolism and enrich Geobacter.
Article
Engineering, Environmental
Wanfa Wang, Si-Liang Li, Jun Zhong, Yuanbi Yi, Fujun Yue, Zenglei Han, Qixin Wu, Ding He, Cong-Qiang Liu
Summary: This study compares the carbon biogeochemical processes in karst and non-karst regions within large thermal stratified river-reservoir systems. The results demonstrate that karst reservoirs have a reduced potential for carbon emissions and highlight the importance of considering geologic settings to improve accuracy in regional and global CO2 emission estimates.
Article
Engineering, Environmental
Chunxia Jiang, Zelong Zhao, Dong Zhu, Xiong Pan, Yuyi Yang
Summary: This study analyzed the occurrence and distribution of antibiotic resistance genes (ARGs) in different environmental media of the Yangtze River using metagenomics. Core resistome dominated by multidrug resistance genes was found in all samples, while rare resistome dominated by various resistance genes was more prevalent in plasmids. Specific bacteria were identified as hosts for both core and rare resistomes, with high clinical concern ARGs found in the rare resistome. Particle-associated environment provided the most ideal conditions for resistome hosts. This study provided insights into the genetic locations of ARGs and the community assembly mechanisms of ARG hosts in freshwater environments.
Article
Engineering, Environmental
Yu Zhang, Yongtao He, Linchun Jia, Lei Xu, Zheng Wang, Yueling He, Ling Xiong, Xumeng Lin, Hong Chen, Gang Xue
Summary: By synergizing organic carbon source, thiosulfate, and zero-valent iron, efficient mixotrophic denitrification of oligotrophic secondary effluent can be achieved. Thiosulfate plays a vital role in promoting TN removal efficiency, while corrosion of Fe0 releases OH- to neutralize H+ from thiosulfate-driven denitrification, creating a suitable environment for denitrification. The coordination of thiosulfate and Fe0 maintains the dominance of Thiobacillus for denitrification.