Article
Microbiology
Chen Yuan, Lidan Sun, Ligong Chen, Limin Li, Zuojun Yao, Yawen Wang, Haiyong Guo, Tanqing Li, Qinye Song
Summary: In this study, Vero cells and IPEC-J2 cells were compared as in vitro models for studying PEDV infection. The results showed that IPEC-J2 cells were more suitable for studying PEDV infection based on their closer cytokine expression profile to in vivo target cells.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Genetics & Heredity
Shiqin Wang, Chao Xu, Jing Shi, Haifei Wang, Shenglong Wu, Wenbin Bao
Summary: This study showed that the expression of APN was upregulated after PEDV infection, and the knockout of APN affected cell proliferation and anti-PEDV activity. Additionally, the SNP rs326030589 was involved in the regulation of APN gene transcription.
Article
Microbiology
Yue Zhang, Huijie Chen, Jia Yu, Rui Feng, Zhao Chen, Xiaolin Zhang, Yudong Ren, Guijun Yang, Xiaodan Huang, Guangxing Li
Summary: This study isolated and characterized an epidemic strain of porcine epidemic diarrhea virus (PEDV) from China and investigated its genomic expression profile in infected pig intestinal cells. The study found that the isolated strain could activate anti-viral innate immune response and inflammation more intensively than a classical strain, suggesting its high pathogenicity. The results contribute to understanding the pathogenesis mechanism of PEDV variants and can aid in the prevention and control of PEDV infection.
VETERINARY MICROBIOLOGY
(2022)
Article
Veterinary Sciences
Qiyuan Luo, Chenglong Zhang, Yun Chen, Huricha Chen, Yuhui Yang
Summary: This study revealed the antiviral mechanism of Alpiniae oxyphyllae fructus polysaccharide 3 (AOFP3) against Porcine epidemic diarrhea virus (PEDV). The results showed that AOFP3 competitively inhibited PEDV adsorption on host cells by blocking the binding of PEDV spike protein to porcine aminopeptidase. Furthermore, AOFP3 decreased PEDV penetration into host cells by reducing cellular cholesterol level.
RESEARCH IN VETERINARY SCIENCE
(2022)
Article
Immunology
Lina Song, Jing Chen, Pengfei Hao, Yuhang Jiang, Wang Xu, Letian Li, Si Chen, Zihan Gao, Ningyi Jin, Linzhu Ren, Chang Li
Summary: This study found that PEDV and TGEV infections can induce differentially expressed genes, especially interferon-stimulated genes. Swine interferon-induced transmembrane protein 3 (sIFITM3) can inhibit PEDV and TGEV infections and has broad-spectrum antiviral activity.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Jiaojiao Yang, Juanli Zhang, Xiaoli Gao, Ruirui Luo, Kaihui Xie, Wei Wang, Jie Li, Qiaoli Yang, Xiaoyu Huang, Zunqiang Yan, Pengfei Wang, Shuangbao Gun
Summary: This study demonstrated the critical role of FTO in infectious diarrhea caused by Clostridium perfringens type C, showing that FTO deficiency promotes apoptosis and inflammation. FTO was found to target Caspase 3, affecting the apoptosis of IPEC-J2 cells. Moreover, m6A modification of mRNA was shown to play a significant role in this process.
Article
Immunology
Jiwen Yang, Gang Tian, Daiwen Chen, Xiangbing Mao, Jun He, Ping Zheng, Jie Yu, Yuheng Luo, Junqiu Luo, Zhiqing Huang, Aimin Wu, Hui Yan, Bing Yu
Summary: Research showed that 1,25(OH)(2)D-3 can inhibit PEDV replication and exert antiviral effects by regulating cell cycle progression through the ERK1/2 signaling pathway.
MICROBIAL PATHOGENESIS
(2021)
Article
Biochemistry & Molecular Biology
Isel Pascual Alonso, Laura Rivera Mendez, Fabiola Almeida Garcia, Mario Ernesto Valdes-Tresanco, Roberto Alonso Bosch, Wilmer H. Perera, Yarini Arrebola Sanchez, Gretchen Bergado, Belinda Sanchez Ramirez, Jean -Louis Charli
Summary: Bufadienolides can selectively inhibit metallo-aminopeptidases, particularly pAPN and pAPA. Telocinobufagin, bufalin, cinobufagin, and bufogenin inhibit the activity of pAPN in a noncompetitive manner, while psi-bufarenogin, cinobufagin, and bufogenin inhibit pAPA in a competitive manner. Despite inducing cell death in MeWo APN+ human melanoma cells, the inhibition of APN does not seem to be the main contributor to the antiproliferative properties of bufadienolides.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Multidisciplinary Sciences
Jie Li, Xiaoyu Huang, Kaihui Xie, Juanli Zhang, Jiaojiao Yang, Zunqiang Yan, Shuangbao Gun
Summary: S100A9 plays an important role in C. perfringens type C-induced infectious diarrhea. Overexpression of S100A9 exacerbates cell damage and inflammatory response, inhibits cell proliferation, and disrupts cell-cell tight junctions. In contrast, inhibition of S100A9 expression alleviates inflammatory injury caused by infectious diarrhea.
Article
Biotechnology & Applied Microbiology
Jie Li, Kaihui Xie, Jiaojiao Yang, Juanli Zhang, Qiaoli Yang, Pengfei Wang, Shuangbao Gun, Xiaoyu Huang
Summary: This study identified downstream target genes regulated by S100A9 in Clostridium perfringens beta2 (CPB2) toxin-induced IPEC-J2 cell injury using RNA-Seq technique. The results showed that S100A9 was involved in CPB2-induced inflammatory response by regulating the expression of downstream target genes, promoting apoptosis, and aggravating oxidative cell damage. This study laid the foundation for further understanding the regulatory mechanism underlying piglet diarrhea.
Article
Biochemistry & Molecular Biology
Chisato Sakuma, Tsuyoshi Sekizuka, Makoto Kuroda, Kentaro Hanada, Toshiyuki Yamaji
Summary: Screening in African green monkey cells identified SYS1 as a crucial gene for STx-induced cell death, impacting glycan biosynthesis through regulation of intra-Golgi and endosome-TGN retrograde transports. This indicates the applicability of the human CRISPR KO library in Vero cell lines.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Genetics & Heredity
Kaihui Xie, Qiaoli Yang, Zunqiang Yan, Xiaoyu Huang, Pengfei Wang, Xiaoli Gao, Shuangbao Gun
Summary: In this study, we identified the involvement of LNC_001186 in CpC-induced diarrhea in piglets, showing its potential as a regulatory factor. Further analyses revealed the coding ability, chromosomal location, and subcellular localization of LNC_001186, as well as its role in regulating CPB2 toxin-induced apoptosis in porcine small intestinal epithelial cells. Overexpression of LNC_001186 was found to inhibit cell apoptosis and promote cell viability.
Article
Microbiology
Jiaojiao Yang, Juanli Zhang, Qiaoli Yang, Xiaoyu Huang, Zunqiang Yan, Pengfei Wang, Xiaoli Gao, Jie Li, Na Li, Yi Gao, Shuangbao Gun
Summary: In this study, a lncRNA called EN-90756 was identified in a CPB2 toxin-treated intestinal porcine epithelial cell line. Functional enrichment analysis revealed that EN-90756 regulated genes associated with antiviral defense and negative regulation of apoptosis. EN-90756 was up-regulated in cells at different time points after CPB2 treatment and its knockdown affected cell proliferation and apoptosis through the JAK-STAT signaling pathway. Moreover, EN-90756 may be involved in CPB2 toxin-induced piglet diarrhea by regulating the expression of the target gene MX1.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Jiwen Yang, Daiwen Chen, Gang Tian, Xiangbing Mao, Jun He, Ping Zheng, Jie Yu, Yuheng Luo, Junqiu Luo, Zhiqing Huang, Aimin Wu, Hui Yan, Bing Yu
Summary: In this study, it was found that 1,25-dihydroxyvitamin D3 (1,25(OH)(2)D-3) can alleviate the inflammatory response induced by PEDV infection by regulating the NF-kappa B and JAK/STAT signaling pathways and inhibiting the expression of inflammatory genes and proteins induced by PEDV infection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Zhenzhen Guo, Chenxin Zhang, Jiajun Dong, Yabin Wang, Hui Hu, Liying Chen
Summary: The interactions between transmissible gastroenteritis virus (TGEV) and Enterococcus faecalis (E. faecalis) were investigated using an in vitro infection model. The results showed that TGEV infection induced epithelial-mesenchymal transition (EMT) of swine intestinal epithelial cells, leading to enhanced cell motility and invasion potential. Additionally, E. faecalis adhered to the cells through the fibronectin (FN) receptor and invaded the cells by binding with the integrin-alpha 5, indicating the critical role of these molecules in the adhesion and invasion of E. faecalis to the cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Infectious Diseases
Pan Qin, Yong-Le Yang, Zhang-Min Hu, Yu-Qi Zhang, Xiao-Qiang Mei, Qi-Zhang Liang, Zongji Lu, Bin Wang, Ruiai Chen, Yao-Wei Huang
Summary: In this study, a novel serological detection method based on PToV envelope spike subunit 1 (S1) protein was developed for the first time, leading to the first seroepidemiological survey of PToV in China. The study revealed a relatively high incidence of PToV seropositivity in pigs of different ages, providing the first serological evidence for PToV infection in pigs from China.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Review
Biochemistry & Molecular Biology
Wan Lu, Zhuangzhuang Zhao, Yao-Wei Huang, Bin Wang
Summary: This review summarizes the research progress on VLPs from different known CoVs, mainly focusing on their characteristics, generation methods, chimerism, and applications in basic research and clinical settings. These studies may contribute to the development of novel VLP vaccines against emerging coronaviruses.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Infectious Diseases
Xiao-Qiang Mei, Pan Qin, Yong-Le Yang, Min Liao, Qi-Zhang Liang, Zhuangzhuang Zhao, Fang-Shu Shi, Bin Wang, Yao-Wei Huang
Summary: This study provides experimental evidence that a mammalian alphacoronavirus, swine acute diarrhea syndrome coronavirus (SADS-CoV), is capable of infecting an avian species, namely chickens.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Microbiology
Ling-Dong Xu, Fei Zhang, Chu Chen, Lei Peng, Wen-Ting Luo, Ruiai Chen, Pinglong Xu, Yao-Wei Huang
Summary: This study revisited the Mongolian gerbil model for human HEV and demonstrated its effectiveness in testing antiviral drugs and assessing the mechanism of host immune response. The availability of this convenient rodent model will greatly aid in HEV-specific antiviral development.
MICROBIOLOGY SPECTRUM
(2022)
Article
Virology
Jin-Hui Zhu, Gaurav Rawal, Ethan Aljets, Wannarat Yim-Im, Yong-Le Yang, Yao-Wei Huang, Karen Krueger, Phillip Gauger, Rodger Main, Jianqiang Zhang
Summary: A 5-plex real-time RT-PCR method was developed for simultaneous detection and differentiation of four swine enteric coronaviruses. The method had high specificity, sensitivity, and repeatability. SADS-CoV was not detected in the tested samples, but PEDV was frequently detected.
Article
Microbiology
Qi-Yue Yang, Yong-Le Yang, Yi-Xin Tang, Pan Qin, Gan Wang, Jin-Yan Xie, Shu-Xian Chen, Chan Ding, Yao-Wei Huang, Shu Jeffrey Zhu
Summary: Bile acids (BAs) play an important role in the infection and replication of swine enteric coronaviruses. Specifically, certain bile acids, such as cholic acid (CA), enhance viral replication by inducing caveolae-mediated endocytosis and endosomal acidification, altering the dynamics of the endosomal/lysosomal system. This study sheds light on the mechanism by which SECoVs exploit microbiome-derived metabolite BAs to establish viral infection rapidly within the intestinal microenvironment.
Article
Immunology
Chu Chen, Ya-Li Li, Fang-Li Lv, Ling-Dong Xu, Yao-Wei Huang
Summary: Although feline coronavirus (FCoV) infection is common in cats, effective treatments are limited. This study developed a recombinant Bacillus subtilis (rBS(CotB-HR2P)) expressing a peptide derived from the heptad repeat 2 domain of the coronavirus spike protein. The study also established a mouse model for FCoV infection and evaluated the protective effects of the oral vaccine candidate. The results suggest that rBS(CotB-HR2P) could be a promising treatment for FECV/FCoV infection in cats.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Microbiology
Fang-Shu Shi, Yin Yu, Ya-Li Li, Lilan Cui, Zhuangzhuang Zhao, Mi Wang, Bin Wang, Rong Zhang, Yao-Wei Huang
Summary: This study systematically analyzed the expression and subcellular localization of 11 distinct viral nonstructural proteins (nsp) during SARS-CoV-2 infection. The data revealed highly regulated expression of nsp genes in space and time, which could provide insights into the function of viral replicases and aid in the development of diagnostics and potential antiviral strategies against SARS-CoV-2.
MICROBIOLOGY SPECTRUM
(2022)
Review
Medicine, Research & Experimental
Tanja Opriessnig, Yao-Wei Huang
Summary: This review article summarizes the latest research findings on whether pigs can be infected with SARS-CoV-2. It suggests that pigs are currently considered a low-risk species, posing no immediate risk to the human population or xenotransplantation recipients. However, monitoring the ever-changing variants of the virus is crucial.
XENOTRANSPLANTATION
(2022)
Article
Gastroenterology & Hepatology
Fei Zhang, Ling-Dong Xu, Qian Zhang, Ailian Wang, Xinyuan Yu, Shengduo Liu, Chu Chen, Shiying Wu, Jianping Jin, Aifu Lin, Dante Neculai, Bin Zhao, Xin-Hua Feng, Tingbo Liang, Pinglong Xu, Yao-Wei Huang
Summary: We identified 17 efficient anti-hepatitis E virus (HEV) drugs through high-throughput screening, which showed promising results in vitro and in vivo. These drugs target the proteostasis of the HEV replicase and exhibited superior efficacy compared to conventional antivirals. This study provides insights into the critical host-HEV interactions and lays the foundation for the development of clinically promising antivirals.
JOURNAL OF HEPATOLOGY
(2023)
Article
Virology
Qi-Zhang Liang, Bin Wang, Chun-Miao Ji, Feifan Hu, Pan Qin, Yaoyu Feng, Yan-Dong Tang, Yao-Wei Huang
Summary: The receptor usage of avian deltacoronaviruses (DCoVs) has been investigated and it has been shown that chicken or porcine aminopeptidase N (APN) serves as a cell receptor for three avian DCoVs. The S1 subunit of these avian DCoVs binds to APN in vitro and on avian and porcine cells. This study provides crucial information on the receptor usage of avian DCoVs and suggests a potential interspecies transmission.
JOURNAL OF VIROLOGY
(2023)
Article
Biochemistry & Molecular Biology
Qian Zhang, Shengduo Liu, Chen-Song Zhang, Qirou Wu, Xinyuan Yu, Ruyuan Zhou, Fansen Meng, Ailian Wang, Fei Zhang, Shasha Chen, Xiaojian Wang, Lei Li, Jun Huang, Yao-Wei Huang, Jian Zou, Jun Qin, Tingbo Liang, Xin-Hua Feng, Sheng-Cai Lin, Pinglong Xu
Summary: This study found that viral infection leads to a rapid decrease in blood glucose levels, resulting in the activation of AMPK. The activated AMPK directly phosphorylates TBK1, triggering the recruitment of IRF3 and the assembly of MAVS or STING signalosomes. However, depletion or inhibition of AMPK and increased glucose levels impair nucleic acid sensing, while enhancing the AMPK-TBK1 cascade significantly improves antiviral immunity.
Article
Biochemistry & Molecular Biology
Zhuangzhuang Zhao, Ling-Dong Xu, Fei Zhang, Qi-Zhang Liang, Yajuan Jiao, Fang-Shu Shi, Biao He, Pinglong Xu, Yao-Wei Huang
Summary: Inhibition of heat shock protein 90 (Hsp90) effectively limits SARS-CoV-2 infection, but little is known about Hsp90 interaction with SARS-CoV-2 proteins. This study analyzed the effects of Hsp90 alpha and Hsp9011 on individual SARSCoV-2 viral proteins and found that five SARS-CoV-2 proteins are novel clients of Hsp9011. Hsp90 depletion-induced N protein degradation is independent of CHIP but alleviated by FBXO10.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Virology
De Yu, Zhuang-Yan Zhao, Yong-Le Yang, Yu Qin, Dan Pan, Li-Xia Yuan, Yao-Wei Huang, Bin Wang
Summary: SADS-CoV is a newly discovered alphacoronavirus that causes diarrhea and vomiting in piglets and has the potential for zoonotic transmission. It is believed to have originated from the bat coronavirus HKU2 and underwent interspecies transmission through an intermediate host. The evolutionary analysis suggests that SADS-CoV is slowly evolving, providing valuable insights for predicting future cross-species transmission and monitoring zoonotic outbreaks.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Veterinary Sciences
Zhuang-Yan Zhao, De Yu, Chun-Miao Ji, Qiankun Zheng, Yao-Wei Huang, Bin Wang
Summary: New analysis suggests that seven arteriviruses isolated from rodents may be ancestors of PRRSV. These viruses share genetic similarities, genome organization, and other characteristics with PRRSV. The evolutionary analysis reveals the close relationship between four rodent arteriviruses and PRRSV, shedding light on the origin and evolution of PRRSV and other arteriviruses.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Virology
Naiqing Xu, Xinen Tang, Xin Wang, Miao Cai, Xiaowen Liu, Xiaolong Lu, Shunlin Hu, Min Gu, Jiao Hu, Ruyi Gao, Kaituo Liu, Yu Chen, Xiufan Liu, Xiaoquan Wang
Summary: This study found that the H9N2 subtype avian influenza virus has a high airborne transmissibility, while the H7N9 virus does not. The Hemagglutinin protein of the H9N2 virus was found to play a key role in replication, stability, and airborne transmission.
Article
Virology
Samar S. Ewies, Sabry M. Tamam, Ahmed S. Abdel-Moneim, Sherin R. Rouby
Summary: Contagious ecthyma (CE) is a highly contagious viral disease of sheep and goats worldwide. The study provided a clinical description of CE and screened for genetic variation in the B2L gene. Infected sheep exhibited anorexia and oral lesions, while inoculated chicken embryos showed pock lesions. The B2L gene was successfully amplified and found to be highly conserved.
Article
Virology
Yigal Farnoushi, Dan Heller, Avishai Lublin
Summary: In recent years, new variants of avian reovirus (ARV) have caused a variety of symptoms in chickens worldwide, including viral arthritis/tenosynovitis. This study analyzed emerging ARV variants in Israel and found significant genetic diversity. Most ARV isolates in Israel belonged to genotypic cluster 5 (GC5). The study suggests that Israel has not experienced the emergence of new ARV variants since the introduction of the live vaccine (ISR-7585), but ongoing monitoring is needed due to the continuous emergence of ARV variants.
Article
Virology
Shigeru Tajima, Michiyo Kataoka, Yuki Takamatsu, Hideki Ebihara, Chang-Kweng Lim
Summary: Yokose virus (YOKV), a bat-associated flavivirus, was found to replicate at a slower rate in mosquito cells compared to other mosquito-borne flaviviruses. Specific nucleotide mutations in the virus were identified to enhance its proliferation ability in mosquito cells.
Article
Virology
Alejandra Borjabad, Baojun Dong, Wei Chao, David J. Volsky, Mary Jane Potash
Summary: This study investigated HIV brain disease using a mouse model, and found that poly I:C can reverse associated cognitive impairment and reduce virus burden. The results also revealed transcriptional changes related to neuronal function and innate immune responses.
Article
Virology
Ching-Hung Lin, Feng-Cheng Hsieh, Meilin Wang, Chieh Hsu, Hsuan-Wei Hsu, Chun-Chun Yang, Cheng-Yao Yang, Hung-Yi Wu
Summary: This study demonstrates that the synthesis of coronavirus subgenomic mRNA is not solely determined by the sequence homology between the leader TRS and TRS-B, but also by the disassociation of the coronavirus polymerase from the viral genome. This finding provides a new insight into the transcription mechanism of coronaviruses.
Article
Virology
Nicholas S. Kron, Benjamin W. Neuman, Sathish Kumar, Patricia L. Blackwelder, Dayana Vidal, Delphina Z. Walker-Phelan, Patrick D. I. Gibbs, Lynne A. Fieber, Michael C. Schmale
Summary: Two recent studies documented the genome of a novel virus in marine animals, finding that the virus is widespread in apparently healthy animals but not highly expressed in neurons. The studies also identified viral replication factories and high levels of defective genomes in chronically infected animals.
Article
Virology
Andrew M. Ramey, Laura C. Scott, Christina A. Ahlstrom, Evan J. Buck, Alison R. Williams, Mia Kim Torchetti, David E. Stallknecht, Rebecca L. Poulson
Summary: We successfully detected and characterized highly pathogenic avian influenza viruses in hunter-harvested wild waterfowl samples from western Alaska. Genomic analysis revealed three independent viral introductions into Alaska. Our findings demonstrate the utility and potential limitations of using molecular processing approaches directly on original swab samples for viral research and monitoring.
Article
Virology
Ting Gong, Dongdong Wu, Yongzhi Feng, Xing Liu, Qi Gao, Xiaoyu Zheng, Zebu Song, Heng Wang, Guihong Zhang, Lang Gong
Summary: This study discovered that quercetin can inhibit PEDV replication both in vivo and in vitro, and alleviate the clinical symptoms and intestinal injury caused by the virus. This provides a new direction for the development of PED antiviral drugs.
Article
Virology
Min Zhu, Hao Zeng, Jianqiao He, Yaohui Zhu, Pingping Wang, Jianing Guo, Jinfan Guo, Huabo Zhou, Yifeng Qin, Kang Ouyang, Zuzhang Wei, Weijian Huang, Ying Chen
Summary: The reassortment between avian H9N2 and Eurasian avian-like (EA) H1N1 viruses may have potentially changed from avian-to-mammals adaptation. This study found that the introduction of EA H1N1 internal genes into H9N2 virus restored the replication capability and resulted in extreme virulence in some cases. This raises new concerns for public health due to the possible coexistence of H9N2 and EA H1N1 viruses in dogs.
