Review
Veterinary Sciences
Savleen Kour, Neelesh Sharma, N. Balaji, Pavan Kumar, Jasvinder Singh Soodan, Marcos Veiga dos Santos, Young-Ok Son
Summary: This article discusses the clinical relevance, causative pathogens, economic factors, diagnostic techniques, and alternative therapeutic protocols of mastitis. The challenges of traditional antibiotic-based therapies require the application of novel therapeutic technologies. Mastitis causes significant economic losses and affects the quality and quantity of milk.
VETERINARY SCIENCES
(2023)
Article
Chemistry, Analytical
Soledad Carinelli, Inigo Fernandez, Jose Luis Gonzalez-Mora, Pedro A. Salazar-Carballo
Summary: This study reports on the fabrication and characterization of novel hemoglobin-modified magnetic nanoparticles for bovine mastitis detection. The nanoparticles were used as solid support for immoblization of the biomarker Haptoglobin, and an electrochemical detection method was applied for quantification. The strategy showed a wide detection range and excellent accuracy, offering a real alternative for on-farm screening of sub-clinical bovine mastitis.
MICROCHEMICAL JOURNAL
(2022)
Article
Agriculture, Dairy & Animal Science
Aarif Ali, Muneeb U. Rehman, Syed Mudasir Ahmad, Tabish Mehraj, Ishraq Hussain, Ahmed Nadeem, Manzoor Ur Rahman Mir, Showkat Ahmad Ganie
Summary: Dairy cattle with a high milk yield are susceptible to subclinical mastitis, and this study focused on the polymorphisms in the bovine transferrin gene for early detection and resistance. The sequencing results revealed nucleotide differences between susceptible cows and disease-free subjects, and in silico tools confirmed the impact on protein structure and function. Phylogenetic analysis showed close relations between the transferrin gene alleles and specific cattle breeds.
Article
Chemistry, Analytical
Soledad Carinelli, Inigo Fernandez, Jose Luis Gonzalez-Mora, Pedro A. Salazar-Carballo
Summary: This study proposes an excellent screening magneto-bioassay for the early detection of bovine subclinical mastitis using haptoglobin (Hp) as a promising biomarker. Chitosan-modified magnetic particles coated with haemoglobin-modified polyaniline were integrated in a sensitive electrochemical Hp bioassay. The optimized electrochemical biosensor showed excellent performance with a dynamic range of 0.001 to 0.32 μg/mL and a detection limit of 0.031 μg/mL, making it highly suitable for subclinical mastitis diagnosis in milk samples.
Article
Veterinary Sciences
Masaru Usui, Misaki Akiyoshi, Akira Fukuda, Hidetomo Iwano, Toshihide Kato
Summary: This study evaluated the use of nanopore sequencing for rapid identification of causative bacteria in bovine mastitis through 16S rRNA analysis. The efficacy of bacterial identification was verified by comparison with conventional culture methods. Nanopore sequencing accurately identified the causative bacteria within approximately 6 hours from the time of sample collection. When the major causative bacteria of bovine mastitis were detected by nanopore sequencing, 98.3% of the results were consistent with identification through conventional culturing methods. 16S rRNA gene analysis using a nanopore sequencer enabled the rapid and accurate identification of bacterial species in bovine mastitis.
RESEARCH IN VETERINARY SCIENCE
(2023)
Article
Microbiology
Verena Roetzer, Jasmin Wenderlein, Anna Wiesinger, Felix Versen, Elke Rauch, Reinhard K. Straubinger, Eva Zeiler
Summary: Bovine udder health is crucial for animal wellbeing and dairy farm economy. The conventional culturing of milk samples is the standard method for diagnosing mastitis, but molecular methods like sequencing offer a deeper insight into bacterial diversity. This study evaluated udder health using both standard methods and 16S rRNA gene amplicon sequencing and found consistent results in subclinical or latent mastitis cases. Molecular biological approaches can provide promising insights into udder pathologies and aid in understanding mastitis incidence.
Article
Medicine, General & Internal
Alexandra Ioana Cardos, Adriana Maghiar, Dana Carmen Zaha, Ovidiu Pop, Luminita Fritea, Florina Miere (Groza), Simona Cavalu
Summary: Rapid diagnosis and treatment in the early stages of H. pylori infection are crucial in preventing its transmission. This review provides an overview of current diagnostic methods for H. pylori and highlights the potential advantages of nanotechnology and lab-on-chip devices in detection.
Article
Biology
Shigenobu Kasai, Ankush Prasad, Ryoma Kumagai, Keita Takanohashi
Summary: In this study, a portable electrochemical device was developed as a novel method for diagnosing mastitis. By measuring the difference in oxygen concentration and the number of somatic cells, mastitis can be accurately diagnosed.
Article
Microbiology
Ajay Kumar Gautam, Rajnish Kumar Verma, Shubhi Avasthi, Sushma, Yogita Bohra, Bandarupalli Devadatha, Mekala Niranjan, Nakarin Suwannarach
Summary: Fungi play a crucial role in different ecosystems and accurately identifying them is essential for mycological research. This compilation provides detailed information on estimates of fungi and highlights the importance of classical and molecular methods. The study also introduces a polyphasic approach for investigating various fungi and discusses the significance of culture-based and culture-independent methods.
Article
Veterinary Sciences
Charlotte Langhorne, Suman Das Gupta, Sara Horsman, Caitlin Wood, Benjamin J. Wood, Leslie Barker, Ania Deutscher, Rochelle Price, Michael R. Mcgowan, Mark Humphris, Shahab Ranjbar, Joerg Henning, Justine S. Gibson
Summary: This study retrospectively analyzed the mastitis-causing organisms isolated from bovine milk samples in Australia. The study identified temporal, geographical, and seasonal patterns of occurrence for these organisms and reported their in vitro susceptibility. The findings emphasized the importance of further research into specific risk factors to improve mastitis control and treatment.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Review
Microbiology
Weicheng Zong, Tianying Zhang, Bing Chen, Qinyue Lu, Xiang Cao, Kun Wang, Zhangping Yang, Zhi Chen, Yi Yang
Summary: This review focuses on the importance and challenges of bovine mastitis, discusses the roles and potential applications of noncoding RNAs (microRNAs and circular RNAs) in this disease, and provides new insights for future research.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Preeti Yadav, Awadh Bihari Yadav, Preksha Gaur, Vartika Mishra, Zul- Huma, Neelesh Sharma, Young-Ok Son
Summary: Mastitis is a devastating economic disease in dairy cattle, commonly occurring during the dry period or early lactation. Escherichia coli and Staphylococcus aureus are the main causative agents of mastitis in India. The use of ciprofloxacin-encapsulated nanocarriers shows potential in improving drug availability and providing an effective treatment for mastitis.
Review
Pediatrics
Cristina Oana Marginean, Lorena Elena Melit, Maria Oana Sasaran
Summary: Helicobacter pylori is a common bacterial infection worldwide that is associated with gastric cancer. Early diagnosis and eradication are key in prevention, with invasive and non-invasive diagnostic methods available. Urea breath tests and stool antigens are particularly useful in clinical practice for monitoring infection and eradication. Factors such as bacterial virulence, environmental influences, and host characteristics must be considered for accurate diagnosis.
Article
Veterinary Sciences
Nikolina Rusenova, Nasko Vasilev, Anton Rusenov, Aneliya Milanova, Ivo Sirakov
Summary: The study aimed to assess the resistance of bovine mastitis S. aureus isolates in Bulgaria to different classes of chemotherapeutic drugs through comparison of phenotypic and genotypic methods. High levels of resistance to sulfadimethoxine were found, along with resistance to other antibiotics. A comprehensive evaluation through standard and modern molecular methods is recommended to address discrepancies in resistance patterns.
VETERINARY SCIENCES
(2022)
Review
Veterinary Sciences
Regitze Renee Pedersen, Volker Kromker, Thomas Bjarnsholt, Kirstin Dahl-Pedersen, Rikke Buhl, Elin Jorgensen
Summary: Bovine mastitis is a significant disease in the dairy industry, with unknown role of biofilm formation in unresolved cases. More in vivo research is needed to uncover the actual impact of biofilm in the pathogenesis of bovine mastitis.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Biotechnology & Applied Microbiology
Aqeela Ashraf, Muhammad Imran, Tahir Yaqub, Muhammad Tayyab, Wasim Shehzad, Claro N. Mingala, Yung-Fu Chang
FOLIA MICROBIOLOGICA
(2018)
Article
Biochemical Research Methods
Aqeela Ashraf, Muhammad Imran, Tahir Yaqub, Muhammad Tayyab, Wasim Shehzad, Peter C. Thomson
MOLECULAR AND CELLULAR PROBES
(2017)
Article
Zoology
Aqeela Ashraf, Muhammad Imran, Yung-Fu Chang
PAKISTAN JOURNAL OF ZOOLOGY
(2018)
Review
Veterinary Sciences
Aqeela Ashraf, Muhammad Imran
ANIMAL HEALTH RESEARCH REVIEWS
(2020)
Article
Veterinary Sciences
Muhammad Luqman Sohail, Muhammad Sarwar Khan, Muhammad Avais, Muhammad Yasir Zahoor, Irfan Khattak, Aqeela Ashraf, Omer Naseer
JOURNAL OF VETERINARY RESEARCH
(2016)