Article
Biotechnology & Applied Microbiology
Nadine Frerker, Tommy A. A. Karlsen, Maria Stensland, Tuula A. A. Nyman, Simon Rayner, Jan E. E. Brinchmann
Summary: Focal lesions of articular cartilage can cause pain and reduced joint function, leading to osteoarthritis if untreated. In vitro generated, scaffold-free autologous cartilage discs made from articular chondrocytes were compared to those made from bone marrow-derived mesenchymal stromal cells. Articular chondrocytes produced more extracellular matrix and contained more articular cartilage proteins, while mesenchymal stromal cells had more proteins associated with cartilage hypertrophy and bone formation. MicroRNA analysis suggested that differential expression of microRNAs played a key role in the differential synthesis of proteins. Therefore, articular chondrocytes are preferred for engineering articular cartilage.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Zhengjie Zhou, Jingtong Zheng, Xiaoting Meng, Fang Wang
Summary: There is increasing evidence that chondrocytes within articular cartilage are affected by endogenous force-related electrical potentials. Furthermore, electrical stimulation (ES) promotes the proliferation of chondrocytes and the synthesis of extracellular matrix (ECM) molecules, which accelerate the healing of cartilage defects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biotechnology & Applied Microbiology
Qian Zhang, Yixin Hu, Xuan Long, Lingling Hu, Yu Wu, Ji Wu, Xiaobing Shi, Runqi Xie, Yu Bi, Fangyuan Yu, Pinxue Li, Yu Yang
Summary: Cartilage regeneration relies on cellular-ECM interactions. Traditional tissue engineering methods have not been able to replicate the physiological structure of natural cartilage, but recent advances in ECM-based scaffolds show promise in improving cartilage regeneration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Engineering, Biomedical
Xiao Xu, Limei Xu, Jiang Xia, Caining Wen, Yujie Liang, Yuanmin Zhang
Summary: Osteoarthritis (OA) is a common disease characterized by cartilage degeneration in middle-aged and elderly people. Currently, there is no effective treatment for OA apart from total joint replacement in advanced stages. Mesenchymal stem cells (MSCs) have shown great potential in regulating the cartilage microenvironment, promoting cartilage regeneration, and alleviating OA symptoms, making them a promising source of cells for OA therapy.
ACTA BIOMATERIALIA
(2023)
Review
Chemistry, Medicinal
Gollahalli Shivashankar Prajwal, Naveen Jeyaraman, Krishna Kanth, Madhan Jeyaraman, Sathish Muthu, Sree Naga Sowndary Rajendran, Ramya Lakshmi Rajendran, Manish Khanna, Eun Jung Oh, Kang Young Choi, Ho Yun Chung, Byeong-Cheol Ahn, Prakash Gangadaran
Summary: Tissue engineering and regenerative medicine provide a new approach for treating musculoskeletal diseases, utilizing MSCs and growth factors to improve the regenerative environment. However, the selection of MSCs source and the long-term and short-term effects of MSCs treatment remain uncertain.
Review
Biochemistry & Molecular Biology
Parviz Vahedi, Rana Moghaddamshahabi, Thomas J. Webster, Ayse Ceren Calikoglu Koyuncu, Elham Ahmadian, Wasim S. Khan, Ali Jimale Mohamed, Aziz Eftekhari
Summary: Cartilage damage is often difficult to repair, and current treatment strategies are inadequate. Autologous mesenchymal stem cells are considered a promising therapeutic approach for cartilage regeneration, as they possess robust cartilaginous production capacity and are easy to isolate and expand without causing morbidity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Vadym Burchak, Fritz Koch, Leonard Siebler, Sonja Haase, Verena K. Horner, Xenia Kempter, G. Bjoern Stark, Ute Schepers, Alisa Grimm, Stefan Zimmermann, Peter Koltay, Sandra Strassburg, Gunter Finkenzeller, Filip Simunovic, Florian Lampert
Summary: This study evaluated the printability and biocompatibility of a semi-synthetic hydrogel system (GelNB/GelS) for bioprinting of adipose-tissue-derived mesenchymal stem cells (ASCs). The results showed that GelNB/GelS hydrogels supported ASCs viability, proliferation and differentiation. The mechanical properties of GelNB/GelS system influenced the differentiation fate of ASCs. The hydrogel system may have potential applications in in vivo tissue engineering.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Biomedical
Cathal D. O'Connell, Serena Duchi, Carmine Onofrillo, Lilith M. Caballero-Aguilar, Anna Trengove, Stephanie E. Doyle, Wiktor J. Zywicki, Elena Pirogova, Claudia Di Bella
Summary: Human articular cartilage has limited self-repair ability, leading to the development of osteoarthritis. Tissue engineering solutions are being researched to regenerate cartilage. The approaches can be categorized as ex situ and in situ strategies, each with its own challenges, opportunities, and prospects for cartilage repair.
ADVANCED HEALTHCARE MATERIALS
(2022)
Review
Engineering, Biomedical
Wenying Wei, Honglian Dai
Summary: Osteochondral defect regeneration remains a challenging issue in the musculoskeletal system, with traditional clinical treatments showing limited efficacy. However, the development of tissue engineering has provided more promising results in regenerating damaged osteochondral tissues.
BIOACTIVE MATERIALS
(2021)
Article
Polymer Science
Esmaiel Jabbari, Azadeh Sepahvandi
Summary: This work presents a novel approach for expanding hMSCs on biomimetic carriers to support tissue regeneration without the need for cell detachment. The results demonstrate that hMSCs expanded using this method exhibited higher expression of chondrogenic markers and compressive moduli in chondrogenic medium compared to those directly encapsulated in gels.
Review
Emergency Medicine
Wenqian Zhang, Jungen Hu, Yizhou Huang, Chenyu Wu, Huiqi Xie
Summary: This review provides an up-to-date overview of the biological characteristics and applications of urine-derived stem cells (USCs) in tissue engineering and regenerative medicine, particularly in skin, bone, and articular cartilage repair. The underlying repair mechanisms and application modes of USCs are summarized, along with potential concerns and perspectives.
Review
Cell Biology
Timothy P. Liu, Pin Ha, Crystal Y. Xiao, Sang Yub Kim, Andrew R. Jensen, Jeremiah Easley, Qingqiang Yao, Xinli Zhang
Summary: There is a need for novel therapeutics for regenerating injured articular cartilage. Mesenchymal stem cells (MSCs) show promise in chondrogenic differentiation and healing, but further investigation in large animal models is needed. Large animal models play a critical role in studying human MSCs in an osteochondral microenvironment and identifying promising approaches for clinical investigation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Engineering, Multidisciplinary
Liangbin Zhou, Peng Guo, Matteo D'Este, Wenxue Tong, Jiankun Xu, Hao Yao, Martin J. Stoddart, Gerjo J. V. M. van Osch, Kevin Ki-Wai Ho, Zhen Li, Ling Qin
Summary: Articular cartilage (AC) is a flexible connective tissue located on the bone surface in the joints. AC defects are common in physically active individuals. Functionalized hydrogels have emerged as promising substitutes for damaged cartilage in AC repair due to their favorable properties. This article introduces the composition, structure, and function of AC and its defects, and reviews the design and fabrication of functionalized hydrogels for AC repair.
Article
Pharmacology & Pharmacy
Asma Sepahdar, Abolfazl Nazbar, Saeed Bahadorikhalili, Ghassem Rezaei, Mohammad Ali Shokrgozar, Mohammad Mehdi Dehghan, Hamid Akbari Javar, Shahin Bonakdar
Summary: This paper presents a novel composite scaffold for the regeneration of cartilage tissue. The scaffold is made of a combination of a hydrogel and a controlled release drug delivery system. The release of kartogenin, a chondrogenic-inducing drug, from the scaffold is successfully controlled. The scaffold also demonstrates a porous structure and shows good performance in inducing chondrogenic differentiation of stem cells.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2022)
Article
Engineering, Biomedical
Shuangpeng Jiang, Guangzhao Tian, Zhen Yang, Xiang Gao, Fuxin Wang, Juntan Li, Zhuang Tian, Bo Huang, Fu Wei, Xinyu Sang, Liuqi Shao, Jian Zhou, Zhenyong Wang, Shuyun Liu, Xiang Sui, Quanyi Guo, Weimin Guo, Xu Li
Summary: This study confirms that hWJMSC-Exos can enhance the effect of the ACECM scaffold and promote osteochondral regeneration. The promoting effect may be related to the polarization of macrophages and inhibition of inflammatory response by hWJMSC-Exos. Additionally, hWJMSC-Exos contain miRNAs that can promote hyaline cartilage regeneration.
BIOACTIVE MATERIALS
(2021)
Article
Cell & Tissue Engineering
Daniel J. Vail, Rodrigo A. Somoza, Arnold Caplan, Ahmad M. Khalil
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2020)
Article
Biochemistry & Molecular Biology
Daniel C. Factor, Anna M. Barbeau, Kevin C. Allan, Lucille R. Hu, Mayur Madhavan, An T. Hoang, Kathryn E. A. Hazel, Parker A. Hall, Sagar Nisraiyya, Fadi J. Najm, Tyler E. Miller, Zachary S. Nevin, Robert T. Karl, Bruna R. Lima, Yanwei Song, Alexandra G. Sibert, Gursimran K. Dhillon, Christina Volsko, Cynthia F. Bartels, Drew J. Adams, Ranjan Dutta, Michael D. Gallagher, William Phu, Alexey Kozlenkov, Stella Dracheva, Peter C. Scacheri, Paul J. Tesar, Olivia Corradin
Review
Cell & Tissue Engineering
Wenchun Qu, Zhen Wang, Joshua M. Hare, Guojun Bu, Jorge M. Mallea, Jorge M. Pascual, Arnold I. Caplan, Joanne Kurtzberg, Abba C. Zubair, Eva Kubrova, Erica Engelberg-Cook, Tarek Nayfeh, Vishal P. Shah, James C. Hill, Michael E. Wolf, Larry J. Prokop, M. Hassan Murad, Fred P. Sanfilippo
STEM CELLS TRANSLATIONAL MEDICINE
(2020)
Article
Multidisciplinary Sciences
Matthew S. Elitt, Lilianne Barbar, H. Elizabeth Shick, Berit E. Powers, Yuka Maeno-Hikichi, Mayur Madhavan, Kevin C. Allan, Baraa S. Nawash, Artur S. Gevorgyan, Stevephen Hung, Zachary S. Nevin, Hannah E. Olsen, Midori Hitomi, Daniela M. Schlatzer, Hien T. Zhao, Adam Swayze, David F. LePage, Weihong Jiang, Ronald A. Conlon, Frank Rigo, Paul J. Tesar
Article
Microbiology
Simote T. Foliaki, Bradley R. Groveman, Jue Yuan, Ryan Walters, Shulin Zhang, Paul Tesar, Wenquan Zou, Cathryn L. Haigh
Article
Cell & Tissue Engineering
Kevin C. Allan, Lucille R. Hu, Marissa A. Scavuzzo, Andrew R. Morton, Artur S. Gevorgyan, Erin F. Cohn, Benjamin L. L. Clayton, Ilya R. Bederman, Stevephen Hung, Cynthia F. Bartels, Mayur Madhavan, Paul J. Tesar
Summary: The study demonstrates that HIF1a impairs oligodendrocyte formation by activating non-canonical targets in OPCs, leading to the suppression of the oligodendrocyte regulator Sox10. Inhibition of MEK/ERK signaling can overcome this block in oligodendrocyte generation by restoring Sox10 expression without affecting canonical HIF1a activity. This research highlights the cell-type-specific HIF1a targets' role in perturbing cell function under low oxygen conditions.
Letter
Immunology
Arnold I. Caplan
ARCHIVUM IMMUNOLOGIAE ET THERAPIAE EXPERIMENTALIS
(2021)
Article
Cell & Tissue Engineering
Tracey L. Bonfield, Morgan T. Sutton, David R. Fletcher, Michael A. Folz, Vaishnavi Ragavapuram, Rodrigo A. Somoza, Arnold I. Caplan
Summary: Chronic nontuberculous mycobacterial infections with Mycobacterium avium and Mycobacterium intracellulare have significant impact on bronchiectasis, chronic obstructive airway disease, and the health of aging individuals. Current treatment regimens with antibiotic macrolides are often toxic and inefficient. This study demonstrates that human marrow-derived mesenchymal stem cells have antimicrobial and anti-inflammatory effects, suggesting a potential therapeutic approach.
STEM CELLS TRANSLATIONAL MEDICINE
(2021)
Review
Genetics & Heredity
Dana Larocca, Jieun Lee, Michael D. West, Ivan Labat, Hal Sternberg
Summary: Multicellular life evolved from replicating unicellular organisms into two different cell types: immortal germline and mortal soma controlled by DNA clocks. Aging is caused by the progressive limitation of growth and regeneration in somatic cells. Reprogramming may lead to therapeutic strategies to alter aging and treat degenerative diseases.
Letter
Multidisciplinary Sciences
Ronald M. Green, Michael D. West, Leonard Hayflick
Article
Cell Biology
Benjamin L. L. Clayton, Paul J. Tesar
Summary: Differentiation of oligodendrocyte progenitor cells into mature oligodendrocytes is crucial for the central nervous system, and disruptions in this process can lead to devastating myelin diseases. New research also suggests a link between perturbed OPC differentiation and neurodegenerative and psychiatric diseases.
CURRENT OPINION IN CELL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Matthew J. Pleshinger, Ryan M. Friedrich, Zita Hubler, Adrianna M. Rivera-Leon, Farrah Gao, David Yan, Joel L. Sax, Ramya Srinivasan, Ilya Bederman, H. Elizabeth Shick, Paul J. Tesar, Drew J. Adams
Summary: Recent studies have uncovered novel links between inhibition of specific sterol pathway enzymes and enhancement of oligodendrocyte formation, offering new insights into potential treatments for diseases such as multiple sclerosis. Genetic suppression of SC4MOL and HSD17B7, as well as the development of potent inhibitors, have been shown to promote the formation of oligodendrocytes, suggesting promising avenues for further research and therapeutic strategies.
RSC CHEMICAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Sophie Martin, Kevin C. Allan, Otis Pinkard, Thomas Sweet, Paul J. Tesar, Jeff Coller
Summary: Mutations in tRNA processing factors can result in myelin disorders, particularly in oligodendrocytes. This study investigates the differences in tRNA modifications and mRNA decay between differentiated oligodendrocytes and precursor cells. The findings suggest that tRNA biology may play a crucial role in oligodendrocyte function, and disruptions in tRNA metabolism can contribute to leukodystrophies and white matter disease.
NATURE COMMUNICATIONS
(2022)
Editorial Material
Medicine, Research & Experimental
Arnold Caplan
Summary: The Commentary introduces cell-based therapy and discusses the potential criteria that the FDA might establish for the approval of clinical trials and eventual market approval of these cell-based therapeutic products. It emphasizes that considering cell-based therapies as single action drugs is inappropriate given their complexity. The regulatory agencies are slowly reevaluating the criteria for allowing clinical trials using cell-based therapies, and this Commentary offers suggestions for modifying the standard criteria.
EXPERIMENTAL BIOLOGY AND MEDICINE
(2023)