Article
Cell Biology
Christine M. Sorenson, Shoujian Wang, Soesiawati R. Darjatmoko, Zafer Gurel, Bo Liu, Nader Sheibani
Summary: TSP1 is a vital regulator of angiogenesis and inflammation in the eye, with its loss contributing to increased retinal vascular density and pathological ocular neovascularization. The study found that while global knockout of TSP1 led to increased retinal vascular density, only lack of TSP1 expression in endothelial cells was sufficient to increase choroidal neovascularization. Individual cell type loss of TSP1 resulted in decreased retinal endothelial cell numbers in a cell type-specific manner, highlighting the unique role TSP1 plays in each cell type.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Ana C. Delgado, Angel R. Maldonado-Soto, Violeta Silva-Vargas, Dogukan Mizrak, Thomas von Kanel, Kelly R. Tan, Alex Paul, Aviv Madar, Henar Cuervo, Jan Kitajewski, Chyuan-Sheng Lin, Fiona Doetsch
Summary: Adult neural stem cells in the ventricular-subventricular zone of mice can generate glial cells in addition to neurons, with certain domains remaining quiescent under normal conditions. Our study also uncovers an intraventricular oligodendrocyte progenitor derived from NSCs that is recruited upon injury.
Review
Neurosciences
Jake M. Cashion, Kaylene M. Young, Brad A. Sutherland
Summary: Multiple sclerosis is a common non-traumatic cause of neurological disability in young adults, characterized by inflammatory demyelination in the central nervous system. Despite the availability of disease-modifying therapies, current treatments are unable to prevent neurodegeneration and disease progression, highlighting the need to understand the underlying cause of multiple sclerosis. Risk factors such as vitamin D deficiency, cigarette smoking, and youth obesity, which also affect vascular health, have been identified. The dysfunction of the neurovascular unit, which regulates neuroinflammation, blood-brain barrier integrity, and cerebral blood flow, may play a crucial role in multiple sclerosis development and progression.
NEUROBIOLOGY OF DISEASE
(2023)
Article
Cell Biology
Chiara Simone Middel, Nadine Dietrich, Hans-Peter Hammes, Jens Kroll
Summary: The retinal trypsin digest protocol provides novel insights into the zebrafish retinal vascular architecture. Quantification of the different cell types shows that zebrafish have higher numbers of mural cells and an increased mural cell to endothelial cell ratio compared to mammalian retina.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Nanoscience & Nanotechnology
Fang Yu, Nivasini Selva Kumar, Satnam Singh, Walter Hunziker, Sum Huan Ng, Deepak Choudhury
Summary: A microfluidic immunopanning chip (MIC) has been developed for simultaneous isolation and purification of multiple types of primary brain microvascular cells, achieving higher efficiency and purity compared to conventional methods. Incorporation of microcarriers in the MIC increases cell capturing efficiency and serves as a substrate for subsequent cell culture. With low reagents consumption, multiplexing capabilities, and high isolation efficiency, the MIC shows great potential for rare cell isolation and broader applications in cell therapy.
MICROFLUIDICS AND NANOFLUIDICS
(2021)
Review
Neurosciences
Tra-My Vu, Vincent Herve, Anosha Kiran Ulfat, Daniel Lamontagne-Kam, Jonathan Brouillette
Summary: The role of non-neuronal cells in Alzheimer's disease (AD) has been overlooked compared to neuronal cells. Recent advances in single-cell/nucleus RNA sequencing and ATAC sequencing have allowed for a better understanding of the function of non-neuronal cells in AD. However, further research is still needed to fully appreciate the interconnected roles of each cell type in the context of AD.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2023)
Article
Biochemical Research Methods
Daniel Spitzer, Maryam I. Khel, Tim Puetz, Jenny Zinke, Xiaoxiong Jia, Kathleen Sommer, Katharina Filipski, Frits Thorsen, Thomas M. Freiman, Stefan Guenther, Karl H. Plate, Patrick N. Harter, Stefan Liebner, Yvonne Reiss, Mariangela Di Tacchio, Sylvaine Guerit, Kavi Devraj
Summary: The neurovascular unit (NVU) is crucial for the proper functioning of the central nervous system and involves endothelial cells, pericytes, astrocytes, microglia, and neurons. Dysfunction of the blood-brain barrier (BBB), regulated by the NVU, is seen in various neurological diseases and is a major therapeutic target. In this study, the EPAM-ia method is introduced as a protocol for simultaneous isolation and analysis of NVU cells using flow cytometry. This method involves the differential processing and combination of NVU cell types followed by immunostaining and fluorescence-activated cell sorting. The EPAM-ia method has applications in transcriptomics, proteomics, and cell culture and can be employed for analyzing various cell types in neurological diseases associated with BBB dysfunction.
Article
Biology
Xiaoyu Xue, Muya Shu, Zhifeng Xiao, Yannan Zhao, Xing Li, Haipeng Zhang, Yongheng Fan, Xianming Wu, Bing Chen, Bai Xu, Yaming Yang, Weiyuan Liu, Sumei Liu, Jianwu Dai
Summary: Nestin is broadly expressed in neural stem/progenitor cells during neural development, but mainly restricted to ependymal cells in adult spinal cord. Following spinal cord injury, Nestin expression is reactivated and Nestin(+) cells aggregate at the injury site. Our study indicates that Nestin(+) cells in the lesion core and edge may be derived from various cell types, with ependymal cells contributing minimally.
SCIENCE CHINA-LIFE SCIENCES
(2022)
Article
Cell Biology
Jeffrey J. Lochhead, Erica I. Williams, Elizabeth S. Reddell, Emma Dorn, Patrick T. Ronaldson, Thomas P. Davis
Summary: The neurovascular unit (NVU) is a group of cells that establish the blood-brain barrier and coordinate cerebral blood flow. Dysfunction of the NVU is common in diseases like ischemic stroke. This study used confocal microscopy to simultaneously investigate associations between different cell types in healthy and ischemic brain tissue, providing a better understanding of NVU function and pathology.
Article
Pharmacology & Pharmacy
Verena Ledwig, Stephan Reichl
Summary: The blood-brain barrier (BBB) is a major challenge for drug development. Suitable in vitro BBB models are important for preclinical development to reduce animal testing and speed up drug discovery. This study aimed to isolate and expand cerebral endothelial cells, pericytes, and astrocytes from porcine brain to create a primary BBB model. Additionally, the study explored immortalized cells as a potential alternative for primary cells. The results showed that the isolated cells, particularly in a triple coculture model, exhibited enhanced barrier integrity, making them valuable for evaluating drug permeation.
Review
Neurosciences
Louis-Philippe Bernier, Clement Brunner, Azzurra Cottarelli, Matilde Balbi
Summary: The neurovascular unit (NVU) in the brain exhibits spatial heterogeneity, with variations in cellular composition and functional specialization across different brain regions. This diversity is crucial for brain function.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Mariela Puebla, Pablo J. Tapia, Hilda Espinoza
Summary: This review focuses on the role of astrocytes in angiogenesis, highlighting their function as scaffolds and stabilizing factors, as well as the phenotypic differences in glioblastoma. Understanding the interaction between endothelial cells and astrocytes is crucial for comprehending the process of angiogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Neurosciences
Fredy Sanchez-Cano, Luisa C. Hernandez-Kelly, Arturo Ortega
Summary: The blood-brain barrier, part of the neurovascular unit, is responsible for maintaining homeostasis within the brain and preventing harmful substances from entering the central nervous system. Dysfunction of the barrier is associated with various neurovascular pathologies. Physiological changes, environmental factors, dietary habits, and psychological stress can all affect the tightness of the barrier.
NEUROTOXICITY RESEARCH
(2021)
Article
Immunology
Alize Proust, Christophe J. J. Queval, Ruth Harvey, Lorin Adams, Michael Bennett, Robert J. J. Wilkinson
Summary: This study investigates the effect of different SARS-CoV-2 variants on brain cells and blood-brain barrier integrity. The results suggest that SARS-CoV-2 is neurotropic and can cause deleterious effects on the central nervous system. The findings provide insights into the potential neurological consequences of SARS-CoV-2 infection.
JOURNAL OF NEUROINFLAMMATION
(2023)
Article
Virology
Michele Bengue, Pauline Ferraris, Jonathan Barthelemy, Cheikh Tidiane Diagne, Rodolphe Hamel, Florian Liegeois, Antoine Nougairede, Xavier de Lamballerie, Yannick Simonin, Julien Pompon, Sara Salinas, Dorothee Misse
Summary: The study indicates that MAYV and CHIKV induce different antiviral responses in astrocytes, with MAYV being more susceptible to the antiviral effects of interferons.
Article
Ophthalmology
Anne-Eva van der Wijk, Paul Canning, Rutger P. van Heijningen, Ilse M. C. Vogels, Cornelis J. F. van Noorden, Ingeborg Klaassen, Reinier O. Schlingemann
ACTA OPHTHALMOLOGICA
(2019)
Article
Biochemistry & Molecular Biology
Anne-Eva van der Wijk, Joanna Wisniewska-Kruk, Ilse M. C. Vogels, Henk A. van Veen, Wing Fung Ip, Nicole N. van der Wel, Cornelis J. F. van Noorden, Reinier O. Schlingemann, Ingeborg Klaassen
Article
Oncology
Robert H. Berndsen, Cedric Castrogiovanni, Andrea Weiss, Magdalena Rausch, Marchien G. Dallinga, Marijana Miljkovic-Licina, Ingeborg Klaassen, Patrick Meraldi, Judy R. van Beijnum, Patrycja Nowak-Sliwinska
BRITISH JOURNAL OF CANCER
(2019)
Article
Clinical Neurology
Anne-Eva van der Wijk, Nadia Lachkar, Judith de Vos, Anita E. Grootemaat, Nicole N. van der Wel, Peter L. Hordijk, Erik N. T. P. Bakker, Ed vanBavel
Article
Multidisciplinary Sciences
Bahar Yetkin-Arik, Ilse M. C. Vogels, Patrycja Nowak-Sliwinska, Andrea Weiss, Riekelt H. Houtkooper, Cornelis J. F. Van Noorden, Ingeborg Klaassen, Reinier O. Schlingemann
SCIENTIFIC REPORTS
(2019)
Article
Biochemistry & Molecular Biology
Marchien G. Dallinga, Yasmin Habani, Richelle P. Kayser, Cornelis J. F. Van Noorden, Ingeborg Klaassen, Reinier O. Schlingemann
MOLECULAR BIOLOGY REPORTS
(2020)
Article
Cell Biology
Marchien G. Dallinga, Yasmin I. Habani, Alinda W. M. Schimmel, Geesje M. Dallinga-Thie, Cornelis J. F. van Noorden, Ingeborg Klaassen, Reinier O. Schlingemann
Summary: The study found that VEGFA(165) and VEGFA(121) have similar inducing effects on tip cells and sprouting in vitro. Co-binding of NRP2 appears to regulate VEGFA-VEGFR2 induced sprout initiation, but not tip cell formation. VLDL plays a role in sprout formation by providing biomass for stalk cell proliferation.
Article
Neurosciences
Theodosia Georgakopoulou, Anne-Eva van der Wijk, Erik N. T. P. Bakker, Ed vanBavel
Summary: The study found that microembolism can lead to ischemia, hypoxia, and infarction, but hypoxic regions can recover over time. This indicates that repair processes in the brain can rescue areas at risk.
JOURNAL OF STROKE & CEREBROVASCULAR DISEASES
(2021)
Article
Ophthalmology
Evy Lefevere, Inge Van Hove, Jurgen Sergeys, David H. W. Steel, Reinier Schlingemann, Lieve Moons, Ingeborg Klaassen
Summary: The study suggests that PDGF may play a role in retinal neurite outgrowth associated with the formation of FVMs in PDR, as indicated by the abundant expression of PDGF receptors in FVMs.
CURRENT EYE RESEARCH
(2022)
Article
Health Care Sciences & Services
Maartje J. C. Vader, Yasmin I. Habani, Reinier O. Schlingemann, Ingeborg Klaassen
Summary: The study found that miR-181a levels were negatively associated with CAT at baseline, but were not associated with the response to anti-VEGF therapy.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Article
Cell Biology
Esmeralda K. Bosma, Shahan Darwesh, Jia Y. Zheng, Cornelis J. F. van Noorden, Reinier O. Schlingemann, Ingeborg Klaassen
Summary: The distribution of VEGFR2 and NRP2 on membrane microdomains in endothelial cells is dynamically regulated depending on growth conditions and the polarity of VEGF-A stimulation.
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY
(2022)
Article
Biochemical Research Methods
Yidan Xue, Theodosia Georgakopoulou, Anne-Eva Van der Wijk, Tamas Jozsa, Ed van Bavel, Stephen J. Payne
Summary: The microvasculature is crucial for oxygen transport in the mammalian brain. Occlusions in cerebral penetrating arteriole trees can lead to unexpected distant tissue hypoxia and infarction. In vivo experiments and in silico simulations were used to investigate the spatial correlation between hypoxic regions and occlusion sites. Results showed that hypoxic regions were present in both experiments and simulations, mainly due to hypoperfusion downstream of the occlusion site. The spatial correlation between hypoxic regions and occlusion sites was reasonably consistent between experiments and simulations.
PLOS COMPUTATIONAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Ingeborg Klaassen, Peter Avery, Reinier O. Schlingemann, David H. W. Steel
Summary: This study investigated the levels of extracellular signaling proteins in patients with proliferative diabetic retinopathy (PDR) of varying severity and after anti-VEGF pre-treatment. The results showed significant differences in vitreous protein levels between PDR patients and controls, with a strong correlation between VEGF, PlGF, MCP1, and ANG2. The study also found that the levels of certain proteins were altered after AFB treatment. These findings suggest that differences in vitreous protein levels may contribute to differences in clinical outcomes in PDR patients.
SCIENTIFIC REPORTS
(2022)