Article
Pharmacology & Pharmacy
Suha M. Dadou, Yiwei Tian, Shu Li, David S. Jones, Gavin P. Andrews
Summary: This study developed predictive inline Process Analytical Technology (PAT) tools for simultaneous quantification of two antihypertensive drugs during continuous manufacture of Fixed-dose combinations (FDCs). The established models showed excellent linearity, selectivity, and accuracy, demonstrating the potential of chemometrics and Raman spectroscopy for in-process quality control of complex formulations.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Review
Engineering, Chemical
Yu Kiat Lin, Hui Yi Leong, Tau Chuan Ling, Dong-Qiang Lin, Shan-Jing Yao
Summary: Downstream processing or product recovery is crucial in bioprocess development, with continuous manufacturing making Process Analytical Technologies (PATs) essential. Spectroscopic methods, particularly Raman spectroscopy, are widely used for real-time monitoring of analytes with minimal sample processing. The innovation of enhanced Raman spectroscopy coupled with chemometrics provides a reliable alternative for downstream process analysis.
CHINESE JOURNAL OF CHEMICAL ENGINEERING
(2021)
Article
Chemistry, Applied
Desiree M. Dalligos, Michael J. Pilling, Georgios Dimitrakis, Liam T. Ball
Summary: The suitability of broadband dielectric spectroscopy (DS) for in-line reaction monitoring is demonstrated, showing high precision and accuracy in measuring reaction progress. A convenient method for rapidly assessing the applicability of DS to previously untested reactions or processes is also established. DS is seen as a valuable addition to the process chemist's analytical toolbox due to its orthogonality to other spectroscopic methods, low cost, and ease of implementation.
ORGANIC PROCESS RESEARCH & DEVELOPMENT
(2023)
Article
Chemistry, Analytical
Kevin Frenkel, Christian Opel, Reinhard Walter, Peter Imming
Summary: This study explored the applications of NIR and Raman spectroscopy in the pharmaceutical industry, with Raman technology found to be superior in determining the decomposition level of sodium hydrogen carbonate. The advantages of thermogravimetric analysis were demonstrated, and the impact of water content, powder density, and particle size on spectra and chemometric models were discussed.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2022)
Article
Engineering, Environmental
Poki Tse, Jenifer Shafer, Samuel A. Bryan, Amanda M. Lines
Summary: The Hanford site presents a complex environmental remediation challenge due to the production of nuclear weapons. Research suggests that the most efficient approach to accurately quantify analytes in the presence of interfering signals is through chemometric modeling, with a focus on partial least square-based models.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2021)
Article
Food Science & Technology
Jakob Forsberg, Per Munk Nielsen, Soren Balling Engelsen, Klavs Martin Sorensen
Summary: Enzymatic degumming is an established process in vegetable oil refinement that offers higher oil yield and more stable downstream processing compared to traditional methods. This study explores the use of Near-Infrared spectroscopy (NIRS) to monitor the enzymatic degumming reaction and proposes a new method to balance spectral noise and maintain chemical information. The optimal Partial Least Squares (PLS) model includes eight wavelength variables and yields an RMSECV of 0.05% free fatty acid using five latent variables, demonstrating the potential for accurate monitoring and prediction of the process.
Article
Biotechnology & Applied Microbiology
Dennis Weber, Juergen Hubbuch
Summary: This study demonstrates the use of Raman spectroscopy as a process analytical technology for monitoring freeze concentration profiles in model formulations containing mAbs or BSA. Freeze concentration was found to be dependent on freezing temperature and formulation, with convection currents observed at the bottom of the freezing container. The study also showed correlation between freeze concentration and sucrose concentration in the formulation, indicating diffusion from the bottom to the top of the container.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Review
Chemistry, Applied
E. J. Rifna, R. Pandiselvam, Anjineyulu Kothakota, K. V. Subba Rao, Madhuresh Dwivedi, Manoj Kumar, Rohit Thirumdas, S. V. Ramesh
Summary: The combination of process analytical tools with chemometrics provides a powerful technique for identifying adulterants in edible oils, with applications in quality monitoring, geographic origin assessment, process evaluation, and product categorization.
Article
Biotechnology & Applied Microbiology
Suyang Wu, Stephanie A. Ketcham, Claudia C. Corredor, Douglas Both, James K. Drennen, Carl A. Anderson
Summary: Cell death is a common failure mode in mammalian cell culture, and apoptosis is the main death process observed. Detecting apoptosis in a timely manner is crucial for preventive control and product quality. This study presents a quantification model using capacitance spectroscopy to measure the percentage of apoptotic cells in an at-line setup, which offers a rapid, simple, and less labor-intensive alternative to traditional methods.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Article
Engineering, Chemical
Lara Julia Lohmann, Jochen Strube
Summary: The research demonstrated the feasibility of online measurement techniques such as Raman spectroscopy within the framework of process analytical technology. By combining DAD and Raman, higher process robustness and accuracy were achieved.
Article
Chemistry, Multidisciplinary
Heather M. Felmy, Nathan P. Bessen, Hope E. Lackey, Samuel A. Bryan, Amanda M. Lines
Summary: In situ and real-time analysis, or online monitoring, of chemical systems has numerous benefits in all fields of chemistry. Matrix effects, where the addition of a new chemical species causes chemical interactions and changes the fingerprints of other chemical species in the system, can pose a common challenge. This study demonstrates this challenge by examining the Raman and visible spectra of the uranyl ion in a mixture of nitric acid and hydrofluoric acid. The application of optical spectroscopy is discussed, along with the use of multivariate curve resolution and HypSpec for speciation analysis. Additionally, chemometric modeling is employed to quantitatively analyze uranium within complex chemical systems with unknown matrix effects.
Article
Biochemistry & Molecular Biology
Hichem Kichou, Emilie Munnier, Yuri Dancik, Kamilia Kemel, Hugh J. Byrne, Ali Tfayli, Dominique Bertrand, Martin Souce, Igor Chourpa, Franck Bonnier
Summary: Confocal Raman microscopy (CRM) coupled with multivariate analysis was used to quantitatively measure active ingredients applied to ex vivo human stratum corneum. The method demonstrated reliability and accuracy by establishing key performance indicators and comparing them to established methodologies. Numerical criteria for analytical performance were determined, and Raman spectroscopy was shown to comply with international guidelines, making it a valuable tool for permeation studies.
Review
Chemistry, Analytical
Wenqiang Pei, Rui Hu, Hao Liu, Le Wang, Yongchao Lai
Summary: An analytical method with high sensitivity and selectivity is needed to understand the impact of nanoplastics. This review article discusses the challenges in nanoplastic analysis using Raman spectroscopy and provides an overview of recent progress in this field. Various techniques, such as hyphenated technology and surface enhanced Raman spectroscopy, are highlighted for their efforts to improve the sensitivity, accuracy, and feasibility of Raman spectroscopy for nanoplastic analysis. The article concludes with a summary of current challenges and future research opportunities in advanced Raman spectroscopy for nanoplastic analysis.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Shivani Choudhary, Deborah Herdt, Erik Spoor, Jose Fernando Garcia Molina, Marcel Nachtmann, Matthias Radle
Summary: This paper attempts to combine future Raman photometers with promising evaluation methods, presenting a new evaluation method that can be applied to measuring concentrations of various liquids with higher accuracy and lower cost.
Article
Biotechnology & Applied Microbiology
Anurag S. Rathore, Saxena Nikita, Garima Thakur, Navnath Deore
Summary: Continuous processing has advantages in high efficiency and cost savings for production of biopharmaceutical products, but faces challenges in implementing complex process control. Real-time monitoring and control are inevitably subject to some limitations.
CURRENT OPINION IN CHEMICAL ENGINEERING
(2021)
Article
Chemistry, Analytical
Qian Wu, Li Tian, Xiangyu Shan, Huiling Li, Chao Li, Juan Lu
Summary: A novel ECL sensor was developed by modifying Fe3CuO4-Ru(bpy)3+ and GO-CdS@ZnS on the electrode for the detection of permethrin. The sensor exhibited high sensitivity and a wide detection range.
Article
Chemistry, Analytical
Tingting Gong, Lei Liao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: In this study, a fluorescent biosensing system for high sensitivity detection of MT was established using a new Ag+-stabilized DNA triplex probe (Ag+-SDTP) combined with catalytic hairpin assembly (CHA) and CRISPR/Cas12a signal enhancements. The sensing method is able to selectively detect MT from other non-specific molecules and can achieve low level detection of MT in diluted human serums, demonstrating its potential for monitoring disease-specific MT biomarker at trace levels.
Article
Chemistry, Analytical
Estrella Sanz Rodriguez, Kyra Wood, Paul R. Haddad, Brett Paull
Summary: This study presents a simple, rapid, and reliable method for determining boron in aqueous solutions using suppressed ion chromatography coupled to electrospray ionisation-triple quadrupole mass spectrometry. The method achieved a low limit of detection and demonstrated excellent analytical performance.
Article
Chemistry, Analytical
Aline Cournut, Paul Moustiez, Yannick Coffinier, Christine Enjalbal, Claudia Bich
Summary: Alzheimer's disease is a major cause of senile dementia and is characterized by the aggregation of neurofibrillary tangles and amyloid plaques in the brain. The A beta 1-42 peptide is considered the primary cause of neurotoxicity due to its insolubility and subsequent aggregation. A beta 1-42 and A beta 1-40 have been identified as relevant biomarkers for AD diagnosis, and SurfaceAssisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS) was used to monitor the A beta 1-42/A beta 1-40 ratio without prior sample treatment.
Article
Chemistry, Analytical
Dotse Selali Chormey, Buse Tugba Zaman, Tulay Borahan Kustanto, Sezin Erarpat Bodur, Suleyman Bodur, Elif Ozturk Er, Sezgin Bakirdere
Summary: This review provides an overview of the harmful effects of endocrine disrupting chemicals (EDCs) on human health and introduces the methods for analyzing EDCs. Recent research has focused on using green chemicals, such as deep eutectic solvents (DESs), for microextraction to ensure environmental safety.
Article
Chemistry, Analytical
Yan Zhang, Renfeng Xu, Jingjing Wu, Zhenghong Zhang, Yuhuang Wang, Hongqin Yang, Sheng Zhang
Summary: This study visualized the pore-like structures in prostate cancer cell membranes after treatment with the anticancer drug paclitaxel, demonstrating its mechanism of action related to oxidative damage.
Article
Chemistry, Analytical
Chihong Ma, Qin Zhou, Jinjin Shi, Hua Gao, Di Huang, Huimin Xue, Han Wang, Zhenzhong Zhang, Sen Yang, Junli Zhang, Kaixiang Zhang
Summary: We developed a CRISPR-empowered electrochemical biosensor, named PER-E-CRISPR, for amplification-free and sensitive detection of miR-21. The biosensor combines the advantages of CRISPR/Cas13a and primer exchange reaction (PER) for target amplification. Under optimized conditions, the PER-E-CRISPR assay exhibits a linear range from 10-13 to 10-7 M and a limit of detection of 30.2 fM. The biosensor shows excellent performance in actual plasma, indicating promising prospects for miRNA detection in molecular diagnosis.
Article
Chemistry, Analytical
Gang Liang, Jie Zhao, Yufei Gao, Tao Xie, Jianhui Zhen, Ligang Pan, Wenwen Gong
Summary: Molecular docking (MD) analysis is widely used for studying the interaction between aptamers and small molecules. This study established steady-state aptamers of tertiary structures (SATS) using specific aptamers of tetracycline antibiotics as docking models. Molecular docking results revealed multiple binding sites in the SATS of aptamers, with significant variations in binding free energy (BFE) and docking score (DS). The study also proposed a method for MD analysis based on SATS, providing insights into binding mode and predicting binding sites.
Article
Chemistry, Analytical
Yixuan Xu, Changchang Teng, Huiping Dang, Dalong Yin, Lifeng Yan
Summary: Stable organic radical molecules with high NIR fluorescence quantum efficiency have been synthesized and encapsulated into nanoparticles, which show great potential for both photodynamic therapy (PDT) and photothermal therapy (PTT) of tumors.
Article
Chemistry, Analytical
Yu Su, Dandan Yang, Yanjie Wang, Jie Ding, Lan Ding, Daqian Song
Summary: In this study, a highly selective molecularly imprinted polymer Cu-MIPs@CS was designed and synthesized for bisphenol A (BPA). Cu-MIPs@CS showed significantly enhanced imprinting factor and selective factor towards BPA compared to MIPs@CS. It also exhibited superior discrimination ability between BPA and its structural analogue. Additionally, Cu-MIPs@CS were successfully applied as a solid phase extraction adsorbent for the determination of BPA in drinking water samples.
Article
Chemistry, Analytical
Yi Qiao, Qiongdan Zhang, Yukun He, Tianguang Cheng, Jing Tu
Summary: Single cell heterogeneity plays an important role in many biological phenomena, and distinguishing cells with specific mutations is beneficial for clinical diagnosis and drug screening. This study introduces a gel plate platform that can distinguish cell types based on their phenotypes, with low equipment requirement. The gel platform has potential in point-of-care circumstances and single-cell stimulation responses that prioritize efficiency and simplicity.
Article
Chemistry, Analytical
Chengke Wang, Shuyang Sun, Ping Wang, Huawei Zhao, Wenling Li
Summary: The food safety of aquatic products is a global concern. Nanotechnology-based analyses have advantages in the detection of bacteria, metal ions, and small molecule contaminants. This review summarizes the recent advances in biosensing strategies for aquatic products and highlights the application of nanomaterials, lateral flow-based biosensors, surface-enhanced Raman scattering, microfluidic chips, and molecular imprinting technologies.
Article
Chemistry, Analytical
Liping Zhu, Zeng Tang, Xuemei Zhang, Li Zhu, Tian Meng, Linying Yu, Ting Xiao, Shasha Lu, Xiaoli Xiong, Xiurong Yang
Summary: A sensitive off-on electrochemiluminescence DNA sensor was developed using Exo III-assisted cascade amplification system. The sensor achieved high selectivity and ultra-high sensitivity in detecting target DNA by utilizing ECL and SPR effects. This study provides a new perspective for designing highly sensitive and programmable ECL biosensors.
Article
Chemistry, Analytical
Tong Li, Mengxu Sun, Suping Xia, Ting Huang, Rong-Tian Li, Chunrong Li, Zong Dai, Jin-Xiang Chen, Jun Chen, Nuan Jia
Summary: Here, we developed a binary system based on DNA tetrahedron and fluorogenic RNA aptamers for highly specific and label-free mRNA imaging in living cells. This system achieved high specificity label-free detection of nucleic acids with a detection limit of 1.34 nM. The method has potential applications in early disease diagnosis and new drug development.
Article
Chemistry, Analytical
Yao Zhao, Shu Zhang, Wang Yao, Yuxuan Zhu, Jing Qian, Juan Yang, Nianjun Yang
Summary: A new hierarchical nanostructured composite with high conductivity and catalytic activity is synthesized. The sensor exhibits significant catalytic effect and high sensitivity for monitoring the environmental endocrine disruptor bisphenol A (BPA).