Article
Biology
Yang Li, Yan Wu, Elena A. Federzoni, Xiaodan Wang, Andre Dharmawan, Xiaoyi Hu, Hui Wang, Robert J. Hawley, Sean Stevens, Megan Sykes, Yong-Guang Yang
Summary: CD47 cross-dressing provides an alternative source of cell surface CD47 that can elicit its anti-phagocytic function without inducing cell death, suggesting a new approach to optimize CD47 transgenic method.
Article
Pharmacology & Pharmacy
Tomoko Ito, Kikuya Sugiura, Aya Hasegawa, Wakana Ouchi, Takayuki Yoshimoto, Izuru Mizoguchi, Toshio Inaba, Katsuyuki Hamada, Masazumi Eriguchi, Yoshiyuki Koyama
Summary: Treatment with tumor-derived EVs showed limited antitumor effect on established tumors due to their immuno-suppressive functions and the poor immunogenicity of TAAs. By genetically modifying parent tumor cells to express a highly immunogenic bacterial antigen, ESAT-6, the EVs exhibited significantly improved antitumor activity in tumor-bearing mice.
Review
Biochemistry & Molecular Biology
Philip W. Askenase
Summary: Carrier effects of extracellular vesicles (EV) like exosomes refer to the properties of the vesicles that contribute to the transferred biologic effects of their contents to targeted cells. These properties include surface activating interactions with targeted cells, potential alternate targets beyond mRNAs, three dimensional aspects of RNAs, EV kinetic dynamics, and unique intracellular release from EV. Understanding these carrier properties can lead to a better understanding of the functional transfers of exosomes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Medicine, General & Internal
Timothy J. Kopper, Xiaoli Yu, Michael W. Graner
Summary: Glioblastomas (GBM) is a devastating disease with poor clinical outcomes. Tumor-derived extracellular vesicles (EVs) impair macrophage function and promote tumor growth. The interaction between macrophages/microglia and gliomas plays a significant role in GBM pathophysiology.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Elisabetta Vulpis, Luisa Loconte, Chiara Cassone, Fabrizio Antonangeli, Giulio Caracciolo, Laura Masuelli, Francesca Fazio, Maria Teresa Petrucci, Cinzia Fionda, Alessandra Soriani, Cristina Cerboni, Marco Cippitelli, Angela Santoni, Alessandra Zingoni
Summary: Natural Killer (NK) cells are innate cytotoxic lymphoid cells that play a crucial role in cancer immunosurveillance. The secretion of NKG2D ligands (NKG2DLs) through extracellular vesicles (EVs) is a mechanism used by cancer cells to evade NKG2D-mediated immunosurveillance. This study investigates the spreading of NKG2DLs through EV-mediated cross-dressing on multiple myeloma (MM) cells and the potential therapeutic implications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Oncology
Andres Rincon-Riveros, Liliana Lopez, E. Victoria Villegas, Josefa Antonia Rodriguez
Summary: Extracellular vesicles, such as exosomes, have the potential to serve as biomarkers for cancer development, impacting various aspects of tumor progression and immune regulation.
Article
Multidisciplinary Sciences
Shizhen Shen, Zhangjin Shen, Conghui Wang, Xiaodong Wu, Lingfang Wang, Lele Ye, Songfa Zhang, Xiaodong Cheng
Summary: In this study, a method for extracting extracellular vesicles (EVs) from epithelial ovarian cancer (EOC) tissues was optimized to investigate the effect of cryopreservation on the phenotypes, contents, and biological functions of the extracted EVs. The results showed that cryopreservation affected the yield and distribution of EVs, but not their morphology or particle mass ratio. The storage of tissues at -80°C increased both small and large EV yields, while the storage of lysates at -80°C decreased large EV yield and increased small EV yield. The cryopreserved EOC tissue retained a high percentage of proteins and miRNAs, and the extracted EVs could promote angiogenesis and invasive migration ability in human endothelial cells. This suggests that cryopreserved EOC tissue has clinical value for studying tissue-derived EVs, especially those related to EV proteins.
Review
Cell Biology
Amy L. Hodge, Amy A. Baxter, Ivan K. H. Poon
Summary: Dendritic cells release extracellular vesicles containing functional molecules that play important roles in immune responses and diseases, and are being explored as potential immunotherapeutic agents.
JOURNAL OF LEUKOCYTE BIOLOGY
(2022)
Article
Medicine, Research & Experimental
Chenggong Tu, Zhimin Du, Hui Zhang, Yueyuan Feng, Yujun Qi, Yongjiang Zheng, Jinbao Liu, Jinheng Wang
Summary: Extracellular vesicles derived from bone marrow stromal cells can promote the cycling of multiple myeloma cells and reduce their sensitivity to bortezomib. Inhibiting endocytosis can decrease vesicle uptake by cells and enhance the anti-myeloma effects of bortezomib.
Review
Biochemistry & Molecular Biology
Maria Jose Moya-Guzman, Javiera de Solminihac, Cristina Padilla, Carolina Rojas, Camila Pinto, Tomas Himmel, Karina Pino-Lagos
Summary: This article reviews the release of small EVs by different types of leukocytes and their implications on cellular homeostasis. The functions of immune cell-derived sEVs and their potential applications in pathological conditions are discussed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Hanzhuang Liu, Shaorui Liu, Yu Xiao, Wenting Song, Huize Li, Lok Wai Cola Ho, Zhen Shen, Chung Hang Jonathan Choi
Summary: This study introduces a pH-reversible boron dipyrromethene (BODIPY) fluorescent probe for confocal imaging of extracellular vesicles (EVs) secreted from living cells. The probe allows visualization of acidic EVs in weakly basic culture medium and intracellular acidic precursor EVs in weakly basic cytoplasm, minimizing false positive signals. Additionally, the joint application of this probe with plasmid transfection reveals the secretion of some EVs from cellular pseudopodia via microtubule trackways, providing mechanistic insights into EV transport.
Review
Parasitology
Xing He, Weiqing Pan
Summary: Understanding host-parasite interactions is crucial for controlling parasitic diseases, and miRNAs play a significant role in these interactions, with cross-species miRNA exchanges being common and important for host defense and parasite survival.
TRENDS IN PARASITOLOGY
(2022)
Article
Environmental Sciences
Celeste K. Carberry, Lauren E. Koval, Alexis Payton, Hadley Hartwell, Yong Ho Kim, Gregory J. Smith, David M. Reif, Ilona Jaspers, M. Ian Gilmour, Julia E. Rager
Summary: This study reveals a potential mechanism through which wildfire exposures induce cardiopulmonary responses, highlighting the role of circulating plasma EVs in intercellular and systems-level communication between tissues.
ENVIRONMENT INTERNATIONAL
(2022)
Review
Chemistry, Multidisciplinary
Silvia Fischer, Elisabeth Deindl
Summary: Extracellular vesicles (EVs) are important for cell-to-cell communication by delivering various RNA, proteins, lipids, and nucleotides. Different types of EVs, including exosomes, cell surface-derived EVs, and apoptotic bodies, contain different RNA species and can be isolated, quantified, and characterized using specific methods.
APPLIED SCIENCES-BASEL
(2021)
Review
Pharmacology & Pharmacy
Jafar Rezaie, Vahid Nejati, Monireh Mahmoodi, Mahdi Ahmadi
Summary: Extracellular vesicles (EVs), particularly MSCs-EVs, have shown great potential as a drug delivery system in nanomedicine. They can selectively deliver therapeutic agents to target cells and improve various diseases. However, there are still challenges that need to be addressed for the clinical translation of modified MSCs-EVs.
BIOCHEMICAL PHARMACOLOGY
(2022)