Review
Agriculture, Multidisciplinary
Haitham G. Abo-Al-Ela
Summary: RNAi is a natural mechanism used to silence specific genes and has been extensively studied and applied in aquaculture, particularly in shrimp. Despite limitations, RNAi methods show potential in therapeutic agents and studying biological functions.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Editorial Material
Oncology
George A. Calin
Summary: The study reveals the functional interaction of intronic miRNAs and ncRNA within the FTX locus in colorectal cancer cells, showing a complex landscape that modulates key signaling pathways for cancer progression.
Article
Biochemistry & Molecular Biology
Xiaoxiao Zhang, Peng Li, Huanhuan Zhong, Fanming Yang, Fanzhou Liu, Gabriel Yedid, Yan Zeng
Summary: Proteins involved in RNA metabolism possess a double-stranded RNA-binding domain (dsRBD), with variations in sequences and functions. A study showed that a short insertion in the dsRBD of Drosha is important for its function, and extending the L1 region in other dsRBDs reduces RNA binding. This highlights the sensitivity of dsRBDs to perturbations in the L1 region and adds to the understanding of their structural and functional diversity.
ACTA BIOCHIMICA ET BIOPHYSICA SINICA
(2021)
Article
Cell Biology
Ricardo C. Abreu, Cristiana Ramos, Clarissa Becher, Miguel Lino, Carlos Jesus, Paula A. da Costa Martins, Patricia A. T. Martins, Maria Joao Moreno, Hugo Fernandes, Lino Ferreira
Summary: The study demonstrates that Exo-Fect is the most efficient method for modulating sEVs, leading to over 1000-fold upregulation of the miRNA of interest and successful delivery of miR-155-5p into target cells, demonstrating its functionality. Furthermore, the membrane alterations in Exo-Fect-loaded sEVs enhance internalization within target cells and decrease interaction with lysosomes.
JOURNAL OF EXTRACELLULAR VESICLES
(2021)
Article
Biotechnology & Applied Microbiology
Yong-Li Zhang, Moorim Kang, Jian-Cheng Wu, Meng-Yao Xie, Ruo-Yan Xue, Qi Tang, Hua Yang, Long-Cheng Li
Summary: This study explored the potential of using small activating RNAs and growth factors to induce the differentiation of human adipose-derived mesenchymal stem cells into hair cell-like cells. The results showed that selected small activating RNAs effectively activated the expression of the ATOH1 gene and induced the differentiation of hair cell progenitor cells into hair cell-like cells. This RNA activation technology could offer a new strategy for hair cell regeneration.
Article
Parasitology
Zhen-Sheng Wang, Hong-Chang Zhou, Chun-Yan Wei, Zhi-Hua Wang, Xiao Hao, Lian-Hui Zhang, Jing-Zhong Li, Zeng-Lei Wang, Heng Wang
Summary: This study investigated small RNAs, particularly tsRNAs and miRNAs, from Trichomonas parasites for the first time. The expression profile of tsRNAs in T. vaginalis was demonstrated, while miRNA was barely detected. These results may promote further research to better understand the evolution of small non-coding RNA in T. vaginalis and their functions in the pathogenesis of trichomoniasis.
PARASITES & VECTORS
(2021)
Review
Microbiology
Asuka Nanbo, Wakako Furuyama, Zhen Lin
Summary: The article provides an overview of the roles of microRNAs in regulating gene expression and the impact of virus-encoded miRNAs on viral replication and immune evasion. It also discusses the persistence of RNA virus-encoded miRNAs in hosts, as well as the potential applications of miRNAs in diagnosis and therapeutics.
FRONTIERS IN MICROBIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
J. Makarova, A. Turchinovich, M. Shkurnikov, A. Tonevitsky
Summary: miRNAs are short RNA molecules that regulate multiple cellular processes through post-transcriptional gene silencing and mediate functional communication between cells in the extracellular space. While it is widely accepted that miRNA transfer between cells occurs via extracellular vesicles (EVs), there are also other carriers of cell-free miRNA.
TRENDS IN BIOCHEMICAL SCIENCES
(2021)
Article
Plant Sciences
Maria C. Holeva, Athanasios Sklavounos, Rajendran Rajeswaran, Mikhail M. Pooggin, Andreas E. Voloudakis
Summary: In this study, it was found that dsRNA treatment can protect plants from infection by Cucumber mosaic virus (CMV), but the effectiveness depends on the source and timing of the dsRNA treatment. In vivo-produced dsRNAs were more effective than the in vitro-produced ones, and dsRNA-CP showed better efficacy than dsRNA-2b.
Article
Biology
Milda Mickute, Kotryna Kvederaviciute, Aleksandr Osipenko, Raminta Mineikaite, Saulius Klimasauskas, Giedrius Vilkaitis
Summary: The study presents a novel chemo-enzymatic approach using RNA 2'-O-methyltransferases to append an adapter to target RNA for sequencing library preparation. This method efficiently identifies RNA species, avoiding misdetection of highly-structured RNA. The findings provide valuable resources for RNA-centered regulatory network studies and could potentially advance the analysis of eukaryotic and prokaryotic ssRNAs.
Review
Cell Biology
Aparimita Das, Harsha Ganesan, Sushmitha Sriramulu, Francesco Marotta, N. R. Rajesh Kanna, Antara Banerjee, Fang He, Asim K. Duttaroy, Surajit Pathak
Summary: Oxidative stress is recognized as a contributing factor to cancer development, while recent research has highlighted the association between cancer genesis and non-coding RNAs (ncRNAs). The introduction of new techniques such as liquid biopsies has significantly improved cancer diagnosis and management.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2021)
Review
Biochemistry & Molecular Biology
Mohammad Aslam, Beenish Fakher, Yuan Qin
Summary: In living organisms, sexual reproduction relies on the successful development of gametes, with flowering plants producing gametes in specialized organs of the flower, the gametophytes. Recent evidence suggests that small RNA-mediated pathways play important roles in the development and specification of the female gametophyte. Numerous protein complexes, molecular, and genetic mechanisms are involved in tightly regulating the development of the female gametophyte, known as the embryo sac, in plants.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Oncology
Maria Teresa Di Martino, Caterina Riillo, Francesca Scionti, Katia Grillone, Nicoletta Polera, Daniele Caracciolo, Mariamena Arbitrio, Pierosandro Tagliaferri, Pierfrancesco Tassone
Summary: Cancer onset and progression are influenced by immune system dysregulation, with noncoding RNAs playing a role in cancer cell immune escape and modulation of the tumor microenvironment. While immunotherapy holds promise for cancer treatment, efforts are needed to achieve cure for immune-refractory tumors.
Article
Engineering, Environmental
Ke Zhang, Joseph Hodge, Anamika Chatterjee, Tae Seok Moon, Kimberly M. Parker
Summary: Double-stranded RNA undergoes alkaline hydrolysis at orders-of-magnitude slower rates compared to single-stranded RNA, and can remain intact for months at neutral pH. Increasing pH effectively attenuates enzymatic degradation of dsRNA without inducing alkaline hydrolysis, which differs significantly from ssRNA. Understanding these unique properties of dsRNA allows for greater control of stability in RNAi technology and provides insights into various applications, including isolation, detection, and inactivation of dsRNA viruses.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Guoping Li, Aidan C. Manning, Alex Bagi, Xinyu Yang, Priyanka Gokulnath, Michail Spanos, Jonathan Howard, Patricia P. Chan, Thadryan Sweeney, Robert Kitchen, Haobo Li, Brice D. Laurent, Sary F. Aranki, Maria Kontaridis, Louise C. Laurent, Kendall Van Keuren-Jensen, Jochen Muehlschlegel, Todd M. Lowe, Saumya Das
Summary: The study highlights the crucial role of transfer RNA-derived small RNAs in different stress responses and introduces a new sequencing technique for generating a comprehensive abundance landscape of cellular and extracellular tDRs. It also reveals that extracellular tDRs have distinct fragmentation signatures which are better indicators of different stress responses than miRNAs.
Article
Evolutionary Biology
Luis M. Vaschetto, Raul E. Gonzalez-Ittig, Julia Vergara, Luis E. Acosta
JOURNAL OF ZOOLOGICAL SYSTEMATICS AND EVOLUTIONARY RESEARCH
(2015)
Article
Biotechnology & Applied Microbiology
Luis Maria Vaschetto
BRIEFINGS IN FUNCTIONAL GENOMICS
(2017)
Article
Biodiversity Conservation
Luis E. Acosta, Luis M. Vaschetto
JOURNAL OF NATURAL HISTORY
(2017)
Article
Agronomy
Luis Maria Vaschetto
MOLECULAR BREEDING
(2016)
Letter
Biochemistry & Molecular Biology
Luis Maria Vaschetto
JOURNAL OF CELLULAR BIOCHEMISTRY
(2018)
Article
Multidisciplinary Sciences
Julia Vergara, Luis E. Acosta, Raul E. Gonzalez-Ittig, Luis M. Vaschetto, Cristina N. Gardenal
Article
Biochemistry & Molecular Biology
Leonardo S. Vanzetti, Nadia Yerkovich, Eugenia Chialvo, Lucio Lombardo, Luis Vaschetto, Marcelo Helguera
GENETICS AND MOLECULAR BIOLOGY
(2013)
Review
Biochemical Research Methods
Luis Maria Vaschetto
PLANT MOLECULAR BIOLOGY REPORTER
(2015)
Editorial Material
Biochemistry & Molecular Biology
Luis Maria Vaschetto
CURRENT PHARMACEUTICAL BIOTECHNOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Luis M. Vaschetto, Natalia Ortiz
Article
Cell Biology
Luis Maria Vaschetto, Hernan Mario Beccacece
WILEY INTERDISCIPLINARY REVIEWS-RNA
(2019)
Editorial Material
Genetics & Heredity
Luis Maria Vaschetto
CURRENT GENE THERAPY
(2019)
Editorial Material
Agronomy
Luis M. Vaschetto
CEREAL GENOMICS: METHODS AND PROTOCOLS
(2020)
Review
Genetics & Heredity
Luis Maria Vaschetto