Article
Biotechnology & Applied Microbiology
Si-Hyeon Kim, Yong-Kuk Kwon, Choi-Kyu Park, Hye-Ryoung Kim
Summary: In this study, metagenomic analysis revealed the presence of C. jejuni and C. psittaci in cockatiels in Korea, which could cause symptoms such as diarrhea. Three de novo assembly tools were compared to obtain accurate contigs, with genetic analysis being performed using sequences generated by metaSPAdes.
Article
Microbiology
Hengchun Cao, Hanxiao Xu, Chunhui Ning, Li Xiang, Qiufang Ren, Tiantian Zhang, Yusen Zhang, Rui Gao
Summary: This study investigated the pangenome of Campylobacter jejuni, analyzing its virulence genes and potential core vaccine targets. Through experiments and analysis, five core vaccine targets with high antigenicity were identified, which play important roles in the virulence of C. jejuni in different hosts. The findings contribute to the discovery of new vaccine targets and provide a method to prevent multidrug-resistant C. jejuni.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Medicine, Research & Experimental
Dantong Sun, Weizheng Wu, Li Wang, Jialin Qu, Qiman Han, Huiyun Wang, Shanai Song, Ning Liu, Yongjie Wang, Helei Hou
Summary: MET fusions are rare in lung cancer patients, with an occurrence rate of 0.2% to 0.3%. In this study, intragenic MET fusions were found in 52.6% of the included patients. Crizotinib was effective in treating MET fusions, including a new type called EML4-MET fusion. This study sheds light on the rarity and therapeutic potential of MET fusions in lung cancer.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Article
Microbiology
Teruyo Ojima-Kato, Satomi Nagai, Akane Fujita, Junko Sakata, Hiroto Tamura
Summary: Identification of microorganisms by MALDI-TOF MS has become popular in the past 20 years. The Strain Solution ver. 2 software with MALDI-TOF MS allows accurate discrimination of serotypes and strains beyond the genus and species level by creating a theoretical mass-based database. This study constructed a theoretical mass database with validated biomarkers for proteotyping Campylobacter jejuni and achieved promising results.
Article
Immunology
Shiho Fukushima, Takaaki Shimohata, Yuri Inoue, Junko Kido, Takashi Uebanso, Kazuaki Mawatari, Akira Takahashi
Summary: Campylobacter jejuni infection activates the Rac1 signaling pathway to promote bacterial internalization, and LC3 plays a role in regulating the invasion signaling of C. jejuni, thereby participating in autophagy-mediated host immune response.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Review
Immunology
Javier Casado, Angel Lanas, Andres Gonzalez
Summary: Two-component regulatory systems (TCRS) are important signal transduction mechanisms in bacteria that play a crucial role in bacterial viability and pathogenicity. H. pylori and C. jejuni, which are classified as high priority pathogens, express various TCRS and orphan response regulators, controlling multiple physiological processes and virulence factors. These microbial molecules hold potential as novel therapeutic targets for the development of new antibiotics.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Infectious Diseases
Kanwal Khan, Zarrin Basharat, Khurshid Jalal, Mutaib M. Mashraqi, Ahmad Alzamami, Saleh Alshamrani, Reaz Uddin
Summary: This study reports the first exploration of drug targets in C. ureolyticus. Through pan-genome analysis and subtractive genomics, 26 druggable proteins were identified. UDP-3-O-acyl-N-acetylglucosamine deacetylase (LpxC) was selected as a target for virtual screening and molecular dynamics simulations were performed for validation.
Article
Multidisciplinary Sciences
Yuli Melisa Sierra-Arguello, Gustavo Perdoncini, Laura Beatriz Rodrigues, Luciana Ruschel dos Santos, Karen Apellanis Borges, Thales Quedi Furian, Carlos Tadeu Pippi Salle, Hamilton Luiz de Souza Moraes, Marcos Jose Pereira Gomes, Vladimir Pinheiro do Nascimento
Summary: Campylobacter jejuni is a common cause of foodborne illnesses worldwide, with limited research on strains isolated from Latin-American countries. This study in Southern Brazil identified 140 C. jejuni strains from various poultry sources and analyzed their virulence markers, providing insight into factors related to the occurrence of this pathogen and their epidemiology. The PCR analysis revealed 25 different virulence profiles, with certain combinations of virulence genes being more common among the strains.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Kanwal Khan, Khurshid Jalal, Yasir Alam, Ghallab Alotaibi, Abdulaziz Al Mouslem, Reaz Uddin, Syed Shah Hassan, Zarrin Basharat
Summary: Campylobacter concisus is a commensal bacterium in the human oral flora and is associated with persistent diarrhea and inflammatory bowel disease (IBD). It is common in children under two years old in developing countries and has been linked to mortality. In developed countries, it is becoming a prevalent cause of bacterial diarrhea in early adulthood. Identifying new therapeutic targets and drugs is crucial for curbing Campylobacter infections. In this study, 18 potential therapeutic candidates were identified, and peptidomimetic inhibitors against deoxycytidine triphosphate deaminase (dCTP deaminase) were screened. Three potent inhibitors were prioritized, and their pharmacokinetics in the human body were simulated to enhance confidence in their effectiveness. These inhibitors hold promise for inhibiting C. concisus.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Pharmacology & Pharmacy
F. D. Lobo de Sa, M. M. Heimesaat, S. Bereswill, P. K. Nattramilarasu, J. D. Schulzke, R. Buecker
Summary: The study shows that resveratrol can improve intestinal epithelial damage caused by Campylobacter jejuni, decrease the rate of epithelial apoptosis, and restore the localization of tight junction proteins in the paracellular domain, thereby enhancing epithelial barrier function.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Microbiology
Martine C. H. Sorensen, Yilmaz Emre Gencay, Florian Fanger, Mariana A. T. Chichkova, Maria Mazurova, Jochen Klumpp, Eva M. Nielsen, Lone Brondsted
Summary: The study revealed that Campylobacter jejuni strains from Danish broilers display varying sensitivity and resistance mechanisms to phages, with different strains being susceptible to specific phages or completely resistant. Furthermore, these strains have developed diverse resistance mechanisms such as CRISPR spacers and restriction modification systems, indicating a complex evolutionary response to phage infection.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Immunology
Luxuan Liu, Jian Feng, Yunming Li, Yugang Liu, Yanyan Wang, Zonghai Hu, Jie Xiong, Qianfei Zuo, Rui Zhang
Summary: This study identified B cell immunodominant epitopes of C. jejuni adhesion protein PEB1, which induced strong B cell mediated humoral immunity response with good immunoprotection efficacy. The immunodominant peptides significantly enhanced opsonize phagocytosis and reduced bacterial burdens and TNF-alpha expression level, showing their potential in anti-C. jejuni vaccine research and development.
MICROBIAL PATHOGENESIS
(2021)
Article
Oncology
Suman Mohajan, Praveen Kumar Jaiswal, Mousa Vatanmakarian, Hassan Yousefi, Saikolappan Sankaralingam, Suresh K. Alahari, Sweaty Koul, Hari K. Koul
Summary: The Hippo pathway serves as a master regulator of various cellular processes and its deregulation is associated with cancer. Despite the importance, the detailed functions of the components of the mammalian Hippo pathway in tumor biology are still unclear.
Article
Veterinary Sciences
A. Carbonero, A. Maldonado-Iniesta, Y. Trujillo, J. Perea, M. Riofrio, I Garcia-Bocanegra, C. Borge
Summary: This study aimed to determine the prevalence of three genes (htrA, htrB, and ppk1) related to environmental persistence in C. jejuni and C. coli isolates obtained from abattoir samples at different stages. The overall prevalence of these genes was 40.3%, 93.3%, and 68.9%, respectively. Significant differences were found between the prevalence in C. jejuni and C. coli, with higher prevalence of htrA and htrB in C. coli, and higher prevalence of ppk1 in C. jejuni. Furthermore, a significant increase in the frequency of htrA and htrB genes in the final meat products compared to initial broiler samples was observed in C. jejuni. These results suggest the involvement of htrA and htrB genes in the environmental persistence of Campylobacter jejuni.
VETERINARY RESEARCH COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Bruce Schultz, Lauren Nicole DeLong, Aliaksandr Masny, Manuel Lentzen, Tamara Raschka, David van Dijk, Andrea Zaliani, Holger Froehlich
Summary: In addition to vaccines, the World Health Organization recognizes the urgency of developing novel medications to combat COVID-19. A machine learning supported web tool called GuiltyTargets-COVID-19 has been developed to identify new candidate drug targets. This tool can prioritize target candidates, assess their druggability, unravel their connection to disease mechanisms, map ligands to targets, and identify potential side effects. Through analyzing RNA-Seq datasets, GuiltyTargets-COVID-19 has identified 4 potential drug targets for COVID-19. Overall, this web tool will facilitate target identification and drug development for COVID-19 in a cell type and tissue specific manner.
SCIENTIFIC REPORTS
(2023)
Article
Agriculture, Dairy & Animal Science
Salma Akter, A. K. M. Mostafa Anower, M. M. Kamal Hossain, Md. Abdul Alim, Nusrat Jahan, Anjuman Ara Bhuyan, Md. Salimullah, Jahangir Alam
Summary: This study aimed to investigate the genetic variants of kappa-casein and beta-lactoglobulin genes in native cattle. Genotyping analysis revealed three genotypes (AA, AB, and BB) for both genes, with the AA genotype being the most common for kappa-casein and the BB genotype being the most common for beta-lactoglobulin. The results indicated a high genetic diversity, but a deviation from Hardy-Weinberg equilibrium, suggesting the presence of selection pressure in the population.
ANIMAL BIOTECHNOLOGY
(2022)
Article
Food Science & Technology
Mahfuzur Rahman, M. A. Islam, Keshob C. Das, Md. Salimullah, M. Z. I. Mollah, Ruhul A. Khan
Summary: The study evaluated the effect of gamma radiation on decontamination of microbial population, physico-chemical properties, radiation sensitivity, and sensory characteristics of common spices for storage. Results showed that gamma radiation significantly reduced microbial load in spices without affecting their physico-chemical, nutritional, and sensory properties.
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
(2021)
Article
Microbiology
Munira Jahan, Arittra Bhattacharjee, Raad Rahmat, S. M. Rashedul Islam, Tahmina Akhter, Ishtiaque Ahammad, Mohammad Uzzal Hossain, Saif Ullah Munshi, M. Salimullah
Summary: The study reports the genome sequences of SARS-CoV-2 viruses collected from SARS-CoV-2-infected patients in Dhaka, Bangladesh during the end phase of the first wave of the COVID-19 pandemic.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2021)
Article
Multidisciplinary Sciences
Ishtiaque Ahammad, Mohammad Uzzal Hossain, Anisur Rahman, Zeshan Mahmud Chowdhury, Arittra Bhattacharjee, Keshob Chandra Das, Chaman Ara Keya, Md Salimullah
Summary: This study analyzed over 1500 SARS-CoV-2 genome sequences from Bangladesh and found that the second wave of the pandemic had a significantly higher mutation rate and more deletion and transversion events compared to the first wave. Different virus lineages were predominant in different waves in Bangladesh, with the B.1.617 variant showing potential for increased transmissibility. Additionally, the study noted the absence of indigenous variants so far in the country, suggesting that preventing the import of foreign variants could help control the outbreak.
Article
Horticulture
Muslima Khatun, Bhabesh Borphukan, Iftekhar Alam, Chaman Ara Keya, Haseena Khan, Malireddy K. Reddy, Md Salimullah
Summary: This study developed improved regeneration and Agrobacterium mediated transformation protocols for eggplant genetic engineering, leading to higher transformation efficiency. By studying key parameters such as culture media composition and hormone concentration, optimal conditions were identified.
SCIENTIA HORTICULTURAE
(2022)
Article
Biochemistry & Molecular Biology
Sheikh Sunzid Ahmed, Abdullah Al-Mamun, Shah Imran Hossain, Farzana Akter, Ishtiaque Ahammad, Zeshan Mahmud Chowdhury, Md Salimullah
Summary: The study aimed to evaluate the antiviral effects of bioactive phytochemicals against SARS-CoV-2 variants of concern. Liquiritigenin, a natural compound, showed the most promising potential as an inhibitor against the variants. These findings provide a potential lead compound for developing effective medications, but further in vivo trials are necessary for validation.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Biotechnology & Applied Microbiology
Keshob Chandra Das, Mohammad Uzzal Hossain, Md Moniruzzaman, Md Salimullah, Sharif Akhteruzzaman
Summary: HMG-CoA reductase (HMGCR) is an important enzyme involved in cholesterol biosynthesis and its study of deleterious mutations and functional single nucleotide polymorphisms (SNPs) can provide insights into the mechanism of hypercholesterolemia and potential therapeutic discoveries.
BIOMED RESEARCH INTERNATIONAL
(2022)
Article
Multidisciplinary Sciences
Zeshan Mahmud Chowdhury, Arittra Bhattacharjee, Ishtiaque Ahammad, Mohammad Uzzal Hossain, Abdullah All Jaber, Anisur Rahman, Preonath Chondrow Dev, Md Salimullah, Chaman Ara Keya
Summary: Streptococcus pneumoniae is the major etiological agent of community-acquired pneumonia and its resistance is increasing. This study identified and analyzed 28 hypothetical proteins that may play a role in the virulence or pathogenesis of the bacteria. The majority of these proteins are involved in bacterial transcription and translation.
Article
Biochemistry & Molecular Biology
Nicola Gossart, Alice Berhin, Kjell Sergeant, Iftekhar Alam, Christelle Andre, Jean-Francois Hausman, Marc Boutry, Charles Hachez
Summary: This study aimed to implement the biosynthesis of triterpenic acids in Nicotiana tabacum glandular trichomes by introducing five transgenes coding for key enzymes involved in the biosynthetic pathway. Analysis of metabolites extracted from entire leaves showed a low amount of ursolic acid in plants expressing the transgenes. These findings suggest that redirecting the terpene biosynthetic pathway in glandular trichomes of N. tabacum can be achieved without detrimental effects on the whole plant phenotype.
Article
Multidisciplinary Sciences
Shashanka Shekhar Sarker, Kh. Muhim Ahmed, Tanzena Tanny, Shamima Nasrin, A. H. M. Mahbubur Rahman, Keshob Chandra Das, Iftekhar Alam
Summary: This study aimed to establish the DNA-based identity and a protocol for high-fidelity micropropagation of Alpinia zerumbet to support the medicinal plant industry. DNA barcode of A. zerumbet was successfully reported through PCR amplification and sequencing of rbcL and matK genes. Microshoots induced from rhizome buds on MS medium supplemented with 6.0 mg L-1 6-benzyl amino purine and 30 g L-1 sucrose rooted well in MS medium amended with 1.5 mg L-1 naphthalene acetic acid. The genetic uniformity of the micropropagated plants was confirmed through the analysis of random amplified polymorphic DNA and inter-simple sequence repeat primers. This research provides an accurate identification method for A. zerumbet and a micropropagation protocol for its economically feasible and environmentally sustainable exploitation.
Article
Chemistry, Multidisciplinary
Mohammad Uzzal Hossain, Ishtiaque Ahammad, Arittra Bhattacharjee, Zeshan Mahmud Chowdhury, Md Tabassum Hossain Emon, Keshob Chandra Das, Chaman Ara Keya, Md Salimullah
Summary: COVID-19, caused by SARS-CoV-2, has resulted in over one million deaths globally, making genome sequencing crucial for identifying genetic variations and insertions/deletions in virus proteins.
Review
Horticulture
Iftekhar Alam, Md Salimullah
Summary: Eggplant is a significant vegetable in Asia and the Mediterranean, but faces limitations in productivity due to pests and environmental stresses. Genetic engineering offers new traits to enhance eggplant, but further research is needed to maximize benefits.
Article
Mathematical & Computational Biology
Atikur Rahman, Tasmina Ferdous Susmi, Farzana Yasmin, Arittra Bhattacharjee, Mohammad Uzzal Hossain, Keshob Chandra Das, Chaman Ara Keya, Md. Salimullah
Summary: This study analyzed the genomic diversity and phylogenetic relations of four dengue virus serotypes found in different regions, revealing close relationships between DENV3 and DENV1 strains, and distinct relationships with the other two strains (DENV2 and DENV4). DENV-2 serotype is considered more virulent with more epidemics of dengue hemorrhagic fever associated. PPIs between dengue virus proteins and its hosts in Bangladesh regions showed that dengue-human PPIs are more prevalent in DENV2 and DENV3 serotypes, while dengue-mosquito PPIs are most predominant in DENV1 serotype.
NETWORK MODELING AND ANALYSIS IN HEALTH INFORMATICS AND BIOINFORMATICS
(2021)
Article
Biotechnology & Applied Microbiology
Mohammad Uzzal Hossain, Arittra Bhattacharjee, Md. Tabassum Hossain Emon, Zeshan Mahmud Chowdhury, Ishtiaque Ahammad, Md. Golam Mosaib, Md. Moniruzzaman, Md. Hadisur Rahman, Md. Nazrul Islam, Irfan Ahmed, Md. Ruhul Amin, Asif Rashed, Keshob Chandra Das, Chaman Ara Keya, Md. Salimullah
Summary: Viral mutations may lead to the development of attenuated vaccines against coronaviruses. Mutations in PLPro may be targeted for the development of better anti-SARS therapeutics.
JOURNAL OF GENETIC ENGINEERING AND BIOTECHNOLOGY
(2021)
