Article
Biotechnology & Applied Microbiology
Guanglian Liao, Lu Chen, Yanqun He, Xishi Li, Zhengxin Lv, Shuyao Yi, Min Zhong, Chunhui Huang, Dongfeng Jia, Xueyan Qu, Xiaobiao Xu
Summary: This study revealed the metabolic characteristics and key enzymes of Ascorbic Acid (AsA) in kiwifruit, as well as identified candidate genes related to AsA biosynthesis, cycling, and degradation through transcriptome analysis. The research results indicate that the accumulation of AsA in fruit mainly depends on the L-galactose pathway.
Article
Horticulture
Qing Liu, Yiqi Li, Guanglian Liao, Xiaobiao Xu, Dongfeng Jia, Min Zhong, Hailing Wang, Bin Ye
Summary: Fruit bagging is an effective method for producing pollution-free fruit, but it may affect the internal quality of the fruit. This study investigated the effects of fruit shading by bagging on kiwifruit quality and identified the molecular mechanisms behind the decrease in ascorbic acid content.
SCIENTIA HORTICULTURAE
(2022)
Article
Biochemistry & Molecular Biology
Huimin Jia, Junjie Tao, Wenqi Zhong, Xudong Jiao, Shuangshuang Chen, Mengting Wu, Zhongshan Gao, Chunhui Huang
Summary: In this study, the metabolites of A. eriantha fruit were analyzed using a metabolomics approach and transcriptome analysis. The biosynthesis and metabolism of phenolic acid, flavonoids, sugars, organic acid and AsA were investigated. The results showed that polyphenols were correlated with specific genes, sugars mainly consisted of sucrose, glucose, arabinose and melibiose, organic acids were mainly citric acid, quinic acid, succinic acid and D-xylonic acid, and the metabolism of AsA acid primarily occurred through the L-galactose pathway. These findings provide valuable insights for kiwifruit molecular breeding.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Juan Luo, Muhammad Abid, Yi Zhang, Xinxia Cai, Jing Tu, Puxin Gao, Zupeng Wang, Hongwen Huang
Summary: This study identified and functionally analyzed SGR family members in kiwifruit, and found their expression characteristics related to light and plant developmental stages. The results provide valuable insights for kiwifruit cultivar development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agriculture, Multidisciplinary
Guang-lian Liao, Chun-hui Huang, Dong-feng Jia, Min Zhong, Jun-jie Tao, Xue-yan Qu, Xiao-biao Xu
Summary: This study reveals the molecular mechanism of high ascorbic acid content in kiwifruit by integrating genomics, metabolomics, and transcriptomics. The results show that ascorbic acid metabolism and the cyclic regeneration pathway play a crucial role in the regulation of ascorbic acid, and the ascorbate peroxidase genes are highly associated with ascorbic acid contents.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2023)
Article
Biochemistry & Molecular Biology
Honghong Deng, Hui Xia, Yuqi Guo, Xinling Liu, Lijin Lin, Jin Wang, Kunfu Xu, Xiulan Lv, Rongping Hu, Dong Liang
Summary: This study determined the dynamic changes in ascorbic acid (AsA) content during fruit development and ripening of Actinidia latifolia and successfully constructed a high-quality, full-length transcriptome of this species. Key genes involved in AsA biosynthesis and recycling pathways were identified, and long noncoding RNAs related to AsA gene expression regulation were also discovered.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Nutrition & Dietetics
Zhenyu Luo, Jieru Zhang, Miaolian Xiang, Jiaoke Zeng, Jinyin Chen, Ming Chen
Summary: Exogenous melatonin can delay the softening and decrease of acidity in postharvest kiwifruit, inhibit the increase of soluble solids content, and maintain a higher content of ascorbic acid during storage. Moreover, it regulates the expression of key genes involved in ascorbic acid metabolism.
FRONTIERS IN NUTRITION
(2022)
Article
Plant Sciences
Guanglian Liao, Qing Liu, Xiaobiao Xu, Yanqun He, Yiqi Li, Hailing Wang, Bin Ye, Chunhui Huang, Min Zhong, Dongfeng Jia
Summary: The study identified an early mature germplasm of Kiwifruit with higher carbohydrate content and lower levels of endogenous hormones, mainly due to a reduced sucrose decomposition rate leading to early harvest.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Agriculture, Multidisciplinary
Guo Xiu-hong, He Yan, Zhang Yu, Wang Yi, Huang Sheng-xiong, Liu Yong-sheng, Li Wei
Summary: This study identified and characterized two cytosolic APX genes (AcAPX1 and AcAPX2) in kiwifruit. The expression pattern and response to NaCl treatment were determined for both AcAPX1 and AcAPX2. The subcellular localization assays showed that both AcAPX1 and AcAPX2 are targeted to the cytosol. Over-expression of AcAPX1 or AcAPX2 increased total ascorbic acid levels and salinity tolerance in Arabidopsis thaliana.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2022)
Article
Plant Sciences
Dongfeng Jia, Zhiqiang Jiang, Haihui Fu, Lu Chen, Guanglian Liao, Yanqun He, Chunhui Huang, Xiaobiao Xu
Summary: This study identified and analyzed 142 NAC transcription factor genes in kiwifruit fruit, and investigated their phylogeny, genomic structure, and expression patterns. The findings provide valuable insights for the future functional identification of NAC genes involved in kiwifruit fruit development.
Article
Food Science & Technology
Mijing Jin, Jianqing Jiao, Qianxi Zhao, Qiuyan Ban, Meng Gao, Jiangtao Suo, Qinggang Zhu, Jingping Rao
Summary: Different concentrations of ABA have different effects on lignification in fruit, with higher concentrations increasing the activity of enzymes crucial for lignin synthesis and upregulating gene expression, while lower concentrations alleviate black patch symptoms and increase lignin content in the peel without negatively affecting fruit quality. Microscope observations showed that lower concentration of ABA can decrease the number of lignin cells in the pulp, reducing the chance of lignification.
Article
Horticulture
Yuanxiu Lin, Bing Zhao, Honglan Tang, Lijuan Cheng, Yunting Zhang, Yan Wang, Jinming Fan, Mengyao Li, Qing Chen, Ya Luo, Xiaorong Wang, Haoru Tang, Yong Zhang
Summary: This study investigated the differences in AsA content and potential mechanisms between two cultivars of hardy kiwifruit. The results showed that GalLDH is a key enzyme for AsA biosynthesis in hardy kiwifruit, while MDHAR is more efficient than DHAR in AsA regeneration. Moreover, AO and APX play important roles in AsA oxidation. This study provides a reference for further genetic improvement and selection of high AsA content materials in cultivation.
SCIENTIA HORTICULTURAE
(2022)
Article
Agronomy
Jiyeon Hyun, Jeong Gu Lee, Kwang-Yeol Yang, Sooyeon Lim, Eun Jin Lee
Summary: In this study, (E)-2-hexenal was used as a natural alternative to artificial fumigants to prevent postharvest pathogenic infection of kiwifruit. The treatment with (E)-2-hexenal enhanced the resistance of kiwifruit to Botrytis cinerea infection by facilitating the signaling network and enabling the accumulation of secondary metabolites. Transcriptome-metabolome analyses showed upregulated expression of defense-related genes and increased biosynthesis of jasmonic acid and flavonoids.
POSTHARVEST BIOLOGY AND TECHNOLOGY
(2022)
Article
Agronomy
Guanglian Liao, Xiaobiao Xu, Chunhui Huang, Xueyan Qu, Dongfeng Jia
Summary: The research team discovered an early-maturing genotype of Actinidia eriantha, 'Ganlv 2', with excellent fruit quality traits, providing a novel genotype for studying the mechanism of early fruit maturity in kiwifruit.
NEW ZEALAND JOURNAL OF CROP AND HORTICULTURAL SCIENCE
(2022)
Article
Multidisciplinary Sciences
Chen Feng, Shuaiyu Zou, Puxin Gao, Zupeng Wang
Summary: This study identified and classified the kiwifruit WOX gene family, revealing differences in WOX gene number and distribution between the Ac and Ae genomes, indicating different evolutionary patterns. Conserved motifs were highly diverged in kiwifruit WOX genes, with functional differentiation identified and potential stress-responsive WOXs determined. The results provided insights into general characteristics, evolutionary patterns, and functional diversity of kiwifruit WOXs.
Article
Plant Sciences
Wanli You, Jinglin Zhang, Xueyin Ru, Feng Xu, Zhengguo Wu, Peng Jin, Yonghua Zheng, Shifeng Cao
Summary: This study investigated the effect of calcium chloride (CaCl2) treatment on GABA accumulation in fresh-cut cantaloupe and the underlying mechanisms. The results showed that CaCl2 treatment increased GABA content and the activities of GAD and SSADH enzymes, while reducing glutamate content and GABA-T activity. Additionally, CaCl2 treatment upregulated the expressions of CmCML11 and CmCAMTA5, as well as several GABA shunt genes, through the transcriptional activation by CmCAMTA5. Furthermore, the interaction between CmCML11 and CmCAMTA5 enhanced the transcriptional activation of GABA shunt genes. Overall, this study reveals that CaCl2 treatment promotes GABA accumulation in fresh-cut cantaloupe through the combined effect of CmCML11 and CmCAMTA5 in regulating the expressions of GABA shunt genes.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2024)
Article
Plant Sciences
Min Wang, Yupeng Wang, Xiaohui Wang, Guangwei Wei, Huiyi Yang, Xi Yang, Tinghai Shen, Huijie Qu, Sheng Fang, Ziming Wu
Summary: This study identified the high nitrogen use efficiency (NUE) black sesame variety 17-156 and analyzed its underlying physiological and molecular mechanisms. The results showed that 17-156 possesses a sophisticated nitrogen metabolizing machinery to uptake and assimilate higher quantities of inorganic nitrogen, simultaneously improving carbon metabolism and growth. Many important genes were up-regulated in 17-156 under high nitrogen condition. Additionally, 38 potential candidate genes were identified for future studies to improve sesame's NUE. These findings provide valuable resources for understanding the regulatory network of nitrogen metabolism and developing sesame cultivars with improved NUE.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2024)
Article
Plant Sciences
He Dong, Chongmei Xu, Chengtao Zhang, Li Zhang, Yaqin Yao, Suiqi Zhang
Summary: The study found that short cells in maize leaves not only improve leaf mechanical support and photosynthetic performance, enhance drought resistance, but also participate in stomatal regulation.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2024)
Article
Plant Sciences
Na Liu, Wenyan Shang, Mengxin Guan, Jibin Xiao, Guangxiang Tian, Baozhan Ma, Wenjing Shang, Xu Li, Shijia Zhao, Chuang Li, Kun Cheng, Wenming Zheng
Summary: This study cloned the full-length cDNA sequence of TaSPX3 gene in wheat and found that TaSPX3 responds to low phosphorus stress in multiple wheat genotypes. Overexpressing TaSPX3 can alleviate phosphorus deficiency symptoms and promote plant growth in Arabidopsis. The study also revealed the interaction of TaSPX3 with other genes related to the phosphorus starvation signaling pathway.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2024)
Article
Plant Sciences
Kongyuan Wu, Lizhen Wang, Zihan Wu, Ziqing Liu, Zengfei Li, Jun Shen, Shengjie Shi, Hong Liu, Christopher Rensing, Renwei Feng
Summary: Selenium (Se) can reduce uptake and translocation of cadmium (Cd) in plants by regulating root morphology. This study investigated the effects of Se(IV) on root exudates, root morphology, root endogenous hormones, and Cd uptake efficiency in rice under Cd stress. The results showed that Se(IV) significantly reduced Cd concentrations in shoots and roots, and decreased Cd uptake efficiency via root hairs. Se(IV) also affected root morphology, root exudates, and the synthesis of hormones like IAA and JA. However, transcriptome analysis revealed no upregulated differentially expressed genes (DEGs) in IAA synthesis.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2024)
Article
Plant Sciences
Di Wang, Huaifang Zhang, Xuefei Hu, Haizhen Zhang, Shuang Feng, Aimin Zhou
Summary: This study identified a cell number regulator gene called SlCNR8 in willow, which enhances resistance to trace metals in transgenic poplar seedlings. SlCNR8 reduces Cd uptake and accumulation, and can be used as a candidate gene for genetic improvement of phytostabilisation of trace metals.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2024)