Chemogenetic Tools for Causal Cellular and Neuronal Biology

Ventral medullary control of rapid eye movement sleep and atonia

(2017) Michael C. Chen et al.   EXPERIMENTAL NEUROLOGY

A Brainstem-Spinal Cord Inhibitory Circuit for Mechanical Pain Modulation by GABA and Enkephalins

(2017) Amaury François et al.   NEURON

AAV-Mediated Anterograde Transsynaptic Tagging: Mapping Corticocollicular Input-Defined Neural Pathways for Defense Behaviors

(2017) Brian Zingg et al.   NEURON

Pharmacogenetic reactivation of the original engram evokes an extinguished fear memory

(2017) Takahiro Yoshii et al.   NEUROPHARMACOLOGY

Layer-specific modulation of neocortical dendritic inhibition during active wakefulness

(2017) William Muñoz et al.   SCIENCE

Deconstructing behavioral neuropharmacology with cellular specificity

(2017) Brenda C. Shields et al.   SCIENCE

Chemogenetics revealed: DREADD occupancy and activation via converted clozapine

(2017) Juan L. Gomez et al.   SCIENCE

Agrp neuron activity is required for alcohol-induced overeating

(2017) Sarah Cains et al.   Nature Communications

Ivermectin-Activated, Cation-Permeable Glycine Receptors for the Chemogenetic Control of Neuronal Excitation

(2016) Robiul Islam et al.   ACS Chemical Neuroscience

A Chemogenetic Receptor That Enhances the Magnitude and Frequency of Glycinergic Inhibitory Postsynaptic Currents without Inducing a Tonic Chloride Flux

(2016) Robiul Islam et al.   ACS Chemical Neuroscience

AgRP Neurons Control Systemic Insulin Sensitivity via Myostatin Expression in Brown Adipose Tissue

(2016) Sophie M. Steculorum et al.   CELL

An Emerging Technology Framework for the Neurobiology of Appetite

(2016) Scott M. Sternson et al.   Cell Metabolism

Parabrachial CGRP Neurons Control Meal Termination

(2016) Carlos A. Campos et al.   Cell Metabolism

Stimulation of the Pontine Parabrachial Nucleus Promotes Wakefulness via Extra-thalamic Forebrain Circuit Nodes

(2016) Mei Hong Qiu et al.   CURRENT BIOLOGY

The ventromedial hypothalamus mediates predator fear memory

(2016) Bianca A. Silva et al.   EUROPEAN JOURNAL OF NEUROSCIENCE

Cre-dependent DREADD (Designer Receptors Exclusively Activated by Designer Drugs) mice

(2016) Hu Zhu et al.   GENESIS

Activation of hypothalamic astrocytes suppresses feeding without altering emotional states

(2016) Patrick Sweeney et al.   GLIA

An Inhibitory Septum to Lateral Hypothalamus Circuit That Suppresses Feeding

(2016) P. Sweeney et al.   JOURNAL OF NEUROSCIENCE

Gi-DREADD Expression in Peripheral Nerves Produces Ligand-Dependent Analgesia, as well as Ligand-Independent Functional Changes in Sensory Neurons

(2016) Jami L. Saloman et al.   JOURNAL OF NEUROSCIENCE

Involvement of mesolimbic dopaminergic network in neuropathic pain relief by treadmill exercise

(2016) Kenta Wakaizumi et al.   Molecular Pain

Naturally occurring p16Ink4a-positive cells shorten healthy lifespan

(2016) Darren J. Baker et al.   NATURE

PV plasticity sustained through D1/5 dopamine signaling required for long-term memory consolidation

(2016) Smitha Karunakaran et al.   NATURE NEUROSCIENCE

A rapidly acting glutamatergic ARC→PVH satiety circuit postsynaptically regulated by α-MSH

(2016) Henning Fenselau et al.   NATURE NEUROSCIENCE

The Rhesus Monkey Connectome Predicts Disrupted Functional Networks Resulting from Pharmacogenetic Inactivation of the Amygdala

(2016) David S. Grayson et al.   NEURON

A Designer AAV Variant Permits Efficient Retrograde Access to Projection Neurons

(2016) D. Gowanlock R. Tervo et al.   NEURON

DREADDs for Neuroscientists

(2016) Bryan L. Roth   NEURON

Mu Opioid Receptor Modulation of Dopamine Neurons in the Periaqueductal Gray/Dorsal Raphe: A Role in Regulation of Pain

(2016) Chia Li et al.   NEUROPSYCHOPHARMACOLOGY

Basal Forebrain Cholinergic Neurons Primarily Contribute to Inhibition of Electroencephalogram Delta Activity, Rather Than Inducing Behavioral Wakefulness in Mice

(2016) Li Chen et al.   NEUROPSYCHOPHARMACOLOGY

Morphine paradoxically prolongs neuropathic pain in rats by amplifying spinal NLRP3 inflammasome activation

(2016) Peter M. Grace et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Gating of hippocampal activity, plasticity, and memory by entorhinal cortex long-range inhibition

(2016) J. Basu et al.   SCIENCE

PET imaging-guided chemogenetic silencing reveals a critical role of primate rostromedial caudate in reward evaluation

(2016) Yuji Nagai et al.   Nature Communications

Genetically and functionally defined NTS to PBN brain circuits mediating anorexia

(2016) Carolyn W. Roman et al.   Nature Communications

Gs-coupled GPCR signalling in AgRP neurons triggers sustained increase in food intake

(2016) Ken-ichiro Nakajima et al.   Nature Communications

Insulin-Dependent Activation of MCH Neurons Impairs Locomotor Activity and Insulin Sensitivity in Obesity

(2016) A. Christine Hausen et al.   Cell Reports

Activation of Pyramidal Neurons in Mouse Medial Prefrontal Cortex Enhances Food-Seeking Behavior While Reducing Impulsivity in the Absence of an Effect on Food Intake

(2016) Daniel M. Warthen et al.   Frontiers in Behavioral Neuroscience

Appetite controlled by a cholecystokinin nucleus of the solitary tract to hypothalamus neurocircuit

(2016) Giuseppe D'Agostino et al.   eLife

Causal Interrogation of Neuronal Networks and Behavior through Virally Transduced Ivermectin Receptors

(2016) Horst A. Obenhaus et al.   Frontiers in Molecular Neuroscience

Tracking the Time-Dependent Role of the Hippocampus in Memory Recall Using DREADDs

(2016) Carmen Varela et al.   PLoS One

Activation of Brainstem Pro-opiomelanocortin Neurons Produces Opioidergic Analgesia, Bradycardia and Bradypnoea

(2016) Serena Cerritelli et al.   PLoS One

Optogenetic and chemogenetic strategies for sustained inhibition of pain

(2016) Shrivats M. Iyer et al.   Scientific Reports

Single and Transient Ca2+ Peaks in Podocytes do not induce Changes in Glomerular Filtration and Perfusion

(2016) Sybille Koehler et al.   Scientific Reports

The First Structure–Activity Relationship Studies for Designer Receptors Exclusively Activated by Designer Drugs

(2015) Xin Chen et al.   ACS Chemical Neuroscience

Gq-DREADD Selectively Initiates Glial Glutamate Release and Inhibits Cue-induced Cocaine Seeking

(2015) Michael D. Scofield et al.   BIOLOGICAL PSYCHIATRY

Hypothalamic Agrp Neurons Drive Stereotypic Behaviors beyond Feeding

(2015) Marcelo O. Dietrich et al.   CELL

Elucidating an Affective Pain Circuit that Creates a Threat Memory

(2015) Sung Han et al.   CELL

Visualizing Hypothalamic Network Dynamics for Appetitive and Consummatory Behaviors

(2015) Joshua H. Jennings et al.   CELL

Palatability Can Drive Feeding Independent of AgRP Neurons

(2015) Raphaël G.P. Denis et al.   Cell Metabolism

Losing Control: Excessive Alcohol Seeking after Selective Inactivation of Cue-Responsive Neurons in the Infralimbic Cortex

(2015) S. Pfarr et al.   JOURNAL OF NEUROSCIENCE

Neurons for hunger and thirst transmit a negative-valence teaching signal

(2015) J. Nicholas Betley et al.   NATURE

Midbrain dopamine neurons bidirectionally regulate CA3-CA1 synaptic drive

(2015) Zev B Rosen et al.   NATURE NEUROSCIENCE

The indirect pathway of the nucleus accumbens shell amplifies neuropathic pain

(2015) Wenjie Ren et al.   NATURE NEUROSCIENCE

Chemogenetic disconnection of monkey orbitofrontal and rhinal cortex reversibly disrupts reward value

(2015) Mark A G Eldridge et al.   NATURE NEUROSCIENCE

A neural basis for melanocortin-4 receptor–regulated appetite

(2015) Alastair S Garfield et al.   NATURE NEUROSCIENCE

Early- and Late-Born Parvalbumin Basket Cell Subpopulations Exhibiting Distinct Regulation and Roles in Learning

(2015) Flavio Donato et al.   NEURON

Dorsal Horn Circuits for Persistent Mechanical Pain

(2015) Cedric Peirs et al.   NEURON

A New DREADD Facilitates the Multiplexed Chemogenetic Interrogation of Behavior

(2015) Eyal Vardy et al.   NEURON

Behavioral and Physiological Effects of a Novel Kappa-Opioid Receptor-Based DREADD in Rats

(2015) Nathan J Marchant et al.   NEUROPSYCHOPHARMACOLOGY

Lateral Hypothalamus GABAergic Neurons Modulate Consummatory Behaviors Regardless of the Caloric Content or Biological Relevance of the Consumed Stimuli

(2015) Montserrat Navarro et al.   NEUROPSYCHOPHARMACOLOGY

Roles of prefrontal cortex and paraventricular thalamus in affective and mechanical components of visceral nociception

(2015) Angela Jurik et al.   PAIN

Gate control of mechanical itch by a subpopulation of spinal cord interneurons

(2015) S. Bourane et al.   SCIENCE

Remote control of therapeutic T cells through a small molecule-gated chimeric receptor

(2015) C.-Y. Wu et al.   SCIENCE

Nicotinic ACh receptors as therapeutic targets in CNS disorders

(2015) Kelly T. Dineley et al.   TRENDS IN PHARMACOLOGICAL SCIENCES

Endogenous Glucagon-like Peptide-1 Suppresses High-Fat Food Intake by Reducing Synaptic Drive onto Mesolimbic Dopamine Neurons

(2015) Xue-Feng Wang et al.   Cell Reports

Astrocytes Control Food Intake by Inhibiting AGRP Neuron Activity via Adenosine A1 Receptors

(2015) Liang Yang et al.   Cell Reports

Cell type-specific pharmacology of NMDA receptors using masked MK801

(2015) Yunlei Yang et al.   eLife

Chemogenetics—A Transformational and Translational Platform

(2015) Justin G. English et al.   JAMA Neurology

Chemogenetic Tools to Interrogate Brain Functions

(2014) Scott M. Sternson et al.   Annual Review of Neuroscience

Leptin Mediates the Increase in Blood Pressure Associated with Obesity

(2014) Stephanie E. Simonds et al.   CELL

A Parabrachial-Hypothalamic Cholecystokinin Neurocircuit Controls Counterregulatory Responses to Hypoglycemia

(2014) Alastair S. Garfield et al.   Cell Metabolism

Chemogenetic Silencing of Neurons in Retrosplenial Cortex Disrupts Sensory Preconditioning

(2014) S. Robinson et al.   JOURNAL OF NEUROSCIENCE

Astrocytic Gq-GPCR-Linked IP3R-Dependent Ca2+ Signaling Does Not Mediate Neurovascular Coupling in Mouse Visual Cortex In Vivo

(2014) D. E. Bonder et al.   JOURNAL OF NEUROSCIENCE

Control of Food Intake and Energy Expenditure by Nos1 Neurons of the Paraventricular Hypothalamus

(2014) A. K. Sutton et al.   JOURNAL OF NEUROSCIENCE

An excitatory paraventricular nucleus to AgRP neuron circuit that drives hunger

(2014) Michael J. Krashes et al.   NATURE

Brainstem nucleus MdV mediates skilled forelimb motor tasks

(2014) Maria Soledad Esposito et al.   NATURE

Leptin-inhibited PBN neurons enhance responses to hypoglycemia in negative energy balance

(2014) Jonathan N Flak et al.   NATURE NEUROSCIENCE

Central amygdala PKC-δ+ neurons mediate the influence of multiple anorexigenic signals

(2014) Haijiang Cai et al.   NATURE NEUROSCIENCE

Designer receptors show role for ventral pallidum input to ventral tegmental area in cocaine seeking

(2014) Stephen V Mahler et al.   NATURE NEUROSCIENCE

Agouti-Related Protein Neuron Circuits That Regulate Appetite

(2014) Scott M. Sternson et al.   NEUROENDOCRINOLOGY

Visual Stimulation Switches the Polarity of Excitatory Input to Starburst Amacrine Cells

(2014) Anna L. Vlasits et al.   NEURON

Chemogenetic Synaptic Silencing of Neural Circuits Localizes a Hypothalamus→Midbrain Pathway for Feeding Behavior

(2014) Tevye J. Stachniak et al.   NEURON

Concurrent and robust regulation of feeding behaviors and metabolism by orexin neurons

(2014) Ayumu Inutsuka et al.   NEUROPHARMACOLOGY

Rat Nucleus Accumbens Core Astrocytes Modulate Reward and the Motivation to Self-Administer Ethanol after Abstinence

(2014) Cecilia Bull et al.   NEUROPSYCHOPHARMACOLOGY

Chemogenetic Inactivation of Ventral Hippocampal Glutamatergic Neurons Disrupts Consolidation of Contextual Fear Memory

(2014) Hu Zhu et al.   NEUROPSYCHOPHARMACOLOGY

Synthetic control of mammalian-cell motility by engineering chemotaxis to an orthogonal bioinert chemical signal

(2014) J. S. Park et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Dendritic Inhibition in the Hippocampus Supports Fear Learning

(2014) M. Lovett-Barron et al.   SCIENCE

A mouse line for inducible and reversible silencing of specific neurons

(2014) Ling Hu et al.   Molecular Brain

AVP neurons in the paraventricular nucleus of the hypothalamus regulate feeding

(2014) Hongjuan Pei et al.   Molecular Metabolism

Rapid versus Delayed Stimulation of Feeding by the Endogenously Released AgRP Neuron Mediators GABA, NPY, and AgRP

(2013) Michael J. Krashes et al.   Cell Metabolism

A Novel Experimental Strategy to Assess the Metabolic Effects of Selective Activation of a Gq-Coupled Receptor in Hepatocytes In Vivo

(2013) Jian Hua Li et al.   ENDOCRINOLOGY

An Engineered Glutamate-gated Chloride (GluCl) Channel for Sensitive, Consistent Neuronal Silencing by Ivermectin

(2013) Shawnalea J. Frazier et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Whole-brain circuit dissection in free-moving animals reveals cell-specific mesocorticolimbic networks

(2013) Michael Michaelides et al.   JOURNAL OF CLINICAL INVESTIGATION

Chronic activation of a designer Gq-coupled receptor improves β cell function

(2013) Shalini Jain et al.   JOURNAL OF CLINICAL INVESTIGATION

Acute and Long-Term Suppression of Feeding Behavior by POMC Neurons in the Brainstem and Hypothalamus, Respectively

(2013) C. Zhan et al.   JOURNAL OF NEUROSCIENCE

Modulation of the autonomic nervous system and behaviour by acute glial cell Gqprotein-coupled receptor activationin vivo

(2013) Cendra Agulhon et al.   JOURNAL OF PHYSIOLOGY-LONDON

Genetic identification of a neural circuit that suppresses appetite

(2013) Matthew E. Carter et al.   NATURE

Parvalbumin-expressing basket-cell network plasticity induced by experience regulates adult learning

(2013) Flavio Donato et al.   NATURE

Inducible control of gene expression with destabilized Cre

(2013) Richard Sando et al.   NATURE METHODS

Transient Inhibition of TrkB Kinase after Status Epilepticus Prevents Development of Temporal Lobe Epilepsy

(2013) Gumei Liu et al.   NEURON

A Cortico-Hippocampal Learning Rule Shapes Inhibitory Microcircuit Activity to Enhance Hippocampal Information Flow

(2013) Jayeeta Basu et al.   NEURON

A Unique Population of Ventral Tegmental Area Neurons Inhibits the Lateral Habenula to Promote Reward

(2013) Alice M. Stamatakis et al.   NEURON

Neuroprotection through Excitability and mTOR Required in ALS Motoneurons to Delay Disease and Extend Survival

(2013) Smita Saxena et al.   NEURON

A Gαs DREADD Mouse for Selective Modulation of cAMP Production in Striatopallidal Neurons

(2013) Martilias S Farrell et al.   NEUROPSYCHOPHARMACOLOGY

Transgenic Mouse Models Enabling Photolabeling of Individual Neurons In Vivo

(2013) Manuel Peter et al.   PLoS One

Role of Orbitofrontal Cortex Neuronal Ensembles in the Expression of Incubation of Heroin Craving

(2012) S. Fanous et al.   JOURNAL OF NEUROSCIENCE

Deconstruction of a neural circuit for hunger

(2012) Deniz Atasoy et al.   NATURE

Optochemical control of genetically engineered neuronal nicotinic acetylcholine receptors

(2012) Ivan Tochitsky et al.   Nature Chemistry

Regulation of neuronal input transformations by tunable dendritic inhibition

(2012) Matthew Lovett-Barron et al.   NATURE NEUROSCIENCE

Imaging the Impact of Chemically Inducible Proteins on Cellular Dynamics In Vivo

(2012) Hon S. Leong et al.   PLoS One

Selective esterase-ester pair for targeting small molecules with cellular specificity

(2012) L. Tian et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Generation of a Synthetic Memory Trace

(2012) A. R. Garner et al.   SCIENCE

Transient activation of specific neurons in mice by selective expression of the capsaicin receptor

(2012) Ali D. Güler et al.   Nature Communications

The Development and Application of Optogenetics

(2011) Lief Fenno et al.   Annual Review of Neuroscience

Ligand-switchable Substrates for a Ubiquitin-Proteasome System

(2011) Emily L. Egeler et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Molecular Determinants of Ivermectin Sensitivity at the Glycine Receptor Chloride Channel

(2011) Timothy Lynagh et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Rapid, reversible activation of AgRP neurons drives feeding behavior in mice

(2011) Michael J. Krashes et al.   JOURNAL OF CLINICAL INVESTIGATION

Optogenetic photochemical control of designer K+ channels in mammalian neurons

(2011) Doris L. Fortin et al.   JOURNAL OF NEUROPHYSIOLOGY

Functional identification of an aggression locus in the mouse hypothalamus

(2011) Dayu Lin et al.   NATURE

AGRP neurons are sufficient to orchestrate feeding behavior rapidly and without training

(2011) Yexica Aponte et al.   NATURE NEUROSCIENCE

Ligand-binding domain of an α7-nicotinic receptor chimera and its complex with agonist

(2011) Shu-Xing Li et al.   NATURE NEUROSCIENCE

Pharmacogenetic Modulation of Orexin Neurons Alters Sleep/Wakefulness States in Mice

(2011) Koh Sasaki et al.   PLoS One

Chemical and Genetic Engineering of Selective Ion Channel-Ligand Interactions

(2011) C. J. Magnus et al.   SCIENCE

A Synthetic Biology Approach Reveals a CXCR4-G13-Rho Signaling Axis Driving Transendothelial Migration of Metastatic Breast Cancer Cells

(2011) H. Yagi et al.   Science Signaling

G protein inhibition of CaV2 calcium channels

(2010) Kevin P.M. Currie   Channels

A General Chemical Method to Regulate Protein Stability in the Mammalian Central Nervous System

(2010) Mari Iwamoto et al.   CHEMISTRY & BIOLOGY

A glycine residue essential for high ivermectin sensitivity in Cys-loop ion channel receptors

(2010) Timothy Lynagh et al.   INTERNATIONAL JOURNAL FOR PARASITOLOGY

An Improved Ivermectin-activated Chloride Channel Receptor for Inhibiting Electrical Activity in Defined Neuronal Populations

(2010) Timothy Lynagh et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Central and Peripheral Neurotoxic Effects of Ivermectin in Rats

(2010) Sasa M TRAILOVIC et al.   JOURNAL OF VETERINARY MEDICAL SCIENCE

The structural basis of function in Cys-loop receptors

(2010) Andrew J. Thompson et al.   QUARTERLY REVIEWS OF BIOPHYSICS

On the Origin of Ion Selectivity in the Cys-Loop Receptor Family

(2009) Steven M. Sine et al.   JOURNAL OF MOLECULAR NEUROSCIENCE

The structure and function of G-protein-coupled receptors

(2009) Daniel M. Rosenbaum et al.   NATURE

Optogenetic dissection of a behavioural module in the vertebrate spinal cord

(2009) Claire Wyart et al.   NATURE

Conditional and reversible disruption of essential herpesvirus proteins

(2009) Mandy Glaß et al.   NATURE METHODS

Targeted disruption of cocaine-activated nucleus accumbens neurons prevents context-specific sensitization

(2009) Eisuke Koya et al.   NATURE NEUROSCIENCE

Remote Control of Neuronal Activity in Transgenic Mice Expressing Evolved G Protein-Coupled Receptors

(2009) Georgia M. Alexander et al.   NEURON

A chemical-genetic approach to study G protein regulation of β cell function in vivo

(2009) Jean-Marc Guettier et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

HaloTag: A Novel Protein Labeling Technology for Cell Imaging and Protein Analysis

(2008) Georgyi V. Los et al.   ACS Chemical Biology

The Role of Gβγ Subunits in the Organization, Assembly, and Function of GPCR Signaling Complexes

(2008) Denis J. Dupré et al.   Annual Review of Pharmacology and Toxicology

Conditional Glycosylation in Eukaryotic Cells Using a Biocompatible Chemical Inducer of Dimerization

(2008) Jennifer L. Czlapinski et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Chemical control of protein stability and function in living mice

(2008) Laura A Banaszynski et al.   NATURE MEDICINE

Engineering GPCR signaling pathways with RASSLs

(2008) Bruce R Conklin et al.   NATURE METHODS

Photochemical control of endogenous ion channels and cellular excitability

(2008) Doris L Fortin et al.   NATURE METHODS

Genetic control of neuronal activity in mice conditionally expressing TRPV1

(2008) Benjamin R Arenkiel et al.   NATURE METHODS

Nitroreductase-mediated cell/tissue ablation in zebrafish: a spatially and temporally controlled ablation method with applications in developmental and regeneration studies

(2008) Silvia Curado et al.   Nature Protocols