Review
Biochemistry & Molecular Biology
Matilde Marini, Mustafa Titiz, Daniel Souza Monteiro de Araujo, Pierangelo Geppetti, Romina Nassini, Francesco De Logu
Summary: Ion channels, specifically the transient receptor potential (TRP) family of channels, play a crucial role in various biological processes, including cancer development and progression. TRP channels have been investigated in different types of cancer and have been associated with processes such as cell proliferation, migration, invasion, angiogenesis, and drug resistance. Understanding the mechanisms by which TRP channels influence cancer provides new opportunities for targeted therapeutic strategies.
Article
Biochemistry & Molecular Biology
Loretta Ferrera, Raffaella Barbieri, Cristiana Picco, Paolo Zuccolini, Alessia Remigante, Sara Bertelli, Maria Rita Fumagalli, Giovanni Zifarelli, Caterina A. M. La Porta, Paola Gavazzo, Michael Pusch
Summary: The response to oxidative stimulation in melanoma cells involves the activation of potassium currents and an increase in intracellular calcium levels, mediated by BK, KCa3.1, and TRPM2 channels. Differences in the expression levels of these channels in different cell lines may play a crucial role in melanoma progression. Targeting these ion channels pharmacologically could be a novel strategy to reduce the metastatic potential of melanoma cells alongside traditional treatments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Neurosciences
Karla M. Marquez-Nogueras, Ivana Y. Kuo
Summary: Calcium release from the endoplasmic reticulum is regulated by ion channel receptors InsP(3)R and RyR, with PC2 playing a less-studied role. PC2 is evolutionarily conserved and mutations in its gene are associated with polycystic kidney disease. However, the functional understanding of PC2 in different subcellular locations and extra-renal cells remains unclear. Recent studies have shed light on its structure and function, especially in the cardiovascular system.
JOURNAL OF PHYSIOLOGY-LONDON
(2023)
Review
Biochemistry & Molecular Biology
Charlotte Van den Eynde, Joris Vriens, Katrien De Clercq
Summary: Calcium ions play a critical role in cell function, with transient receptor potential (TRP) channels serving as a type of ion channel that can convey various stimuli and potentially participate in cellular responses to changing environments.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2021)
Review
Cell Biology
Akito Nakao, Yoshihiro Matsunaga, Katsumi Hayashida, Nobuaki Takahashi
Summary: Psychiatric disorders are believed to be caused by complex and diverse factors, with oxidative stress playing a crucial role in the pathogenesis of major disorders such as bipolar disorder, depression, anxiety disorder, and schizophrenia. Calcium channels are essential for neuronal functions, and the investigation of redox-sensitive TRP channels could lead to the development of new therapeutic strategies for psychiatric disorders.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Etienne de Coulon, Christian Dellenbach, Stephan Rohr
Summary: The LSSE system allows for comprehensive intracellular electrical recordings on cells undergoing strain events, providing stable conditions for studying mechanically activated ion currents. This system offers advanced insights into mechanosensitive ion channel function and allows for quantitative comparisons among different types of channels and cells.
Article
Cell Biology
Karla M. Marquez-Nogueras, Virdjinija Vuchkovska, Elisabeth DiNello, Sara Osorio-Valencia, Ivana Y. Kuo
Summary: Polycystin-2 (PC2) plays a crucial role in myogenesis by regulating intracellular Ca2+ signals and metabolic pathways, affecting the differentiation and mitochondrial remodeling of myoblasts.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Leo C. T. Ng, Brandon J. Harris, Megan Larmore, My C. Ta, Thuy N. Vien, Valerie L. Tokars, Vladimir Yarov-Yarovoy, Paul G. DeCaen
Summary: In this study, computational prediction tools were used to model missing PKD2L1 structural motifs and evaluate over 150 mutations in the entire pore module. Results revealed an energetic landscape of the polycystin pore and identified critical structural regulators controlling the conductive and nonconductive states of the polycystin ion channel.
Article
Medicine, Research & Experimental
Zoltan Petho, Karolina Najder, Stephanie Beel, Benedikt Fels, Ilka Neumann, Sandra Schimmelpfennig, Sarah Sargin, Maria Wolters, Klavs Grantins, Eva Wardelmann, Miso Mitkovski, Andrea Oeckinghaus, Albrecht Schwab
Summary: This study reveals how the unique pH landscape during the progression of pancreatic ductal adenocarcinoma (PDAC) affects pancreatic stellate cells (PSCs) and cancer-associated fibroblasts (CAFs), leading to PDAC fibrosis.
Review
Biochemistry & Molecular Biology
Dorina Zoephel, Chantal Hof, Annette Lis
Summary: Aging is an unstoppable process that affects every cell of the organism, especially the changes in Ca2+ signaling in immune cells which may profoundly impact the development of age-related diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Physiology
Kunal R. Shah, Xin Guan, Jiusheng Yan
Summary: Ion channels can form macromolecular signaling complexes to ensure accurate signal transduction. The BK channel limits increases in [Ca2+]i levels through K+ efflux and rapid membrane repolarization, serving as a negative feedback regulator.
FRONTIERS IN PHYSIOLOGY
(2022)
Review
Cell Biology
Sung-Min Hwang, Ji Yeon Lee, Chul-Kyu Park, Yong Ho Kim
Summary: Neurodegenerative diseases (NDs) primarily affect neurons and glial cells in the brain, with risk factors and pathophysiological mechanisms not fully understood. Homeostasis of intracellular Ca2+ concentration and pH is crucial for cell function, with TRP channels and PMCA playing vital roles in modulating ionic homeostasis. Dysfunctions of TRP channels and PMCA disrupt neuronal homeostasis under pathological conditions, resulting in various NDs. This review highlights their potential as novel targets for treating NDs.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Yusheng Liu, Yihan Lyu, Hongmei Wang
Summary: Transient receptor potential (TRP) channels, widely expressed on the plasma membrane of cells, play an essential role as sensors in the physiological and pathological processes of cells, regulating secretion, interfering with cell proliferation, and affecting neural activity.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Anesthesiology
Mengmeng Zhao, Lei Liu, Zhenghao Chen, Ning Ding, Jiliang Wen, Jiaxin Liu, Nan Ge, Xiulin Zhang
Summary: TRPM3 channel plays an important role in the development of bladder pain in IC/PBS, and targeting TRPM3 may be a pharmacological strategy for treating bladder pain.
Article
Multidisciplinary Sciences
Viviane Nascimento Da Conceicao, Yuyang Sun, Karthik Ramachandran, Arun Chauhan, Amritha Raveendran, Manigandan Venkatesan, Bony DeKumar, Soumya Maity, Neelanjan Vishnu, George A. Kotsakis, Paul F. Worley, Donald L. Gill, Bibhuti B. Mishra, Muniswamy Madesh, Brij B. Singh
Summary: The transformation of naive macrophages into M1 or M2 phenotypes is regulated by distinct Ca2+ entry channels. Blocking Ca2+ entry can suppress signaling events related to cytokine production and reduce the inflammatory response of macrophages, depending on the expression levels of Orai1 and TRPC1 channels.
Meeting Abstract
Biophysics
Richard Banh, Kethika Kulleperuma, Vladimir V. Cherny, Deri Morgan, Boris Musset, Sarah Thomas, Susan M. E. Smith, Regis Pomes, Thomas E. DeCoursey
BIOPHYSICAL JOURNAL
(2019)
Article
Multidisciplinary Sciences
Richard Banh, Vladimir V. Cherny, Deri Morgan, Boris Musset, Sarah Thomas, Kethika Kulleperuma, Susan M. E. Smith, Regis Pomes, Thomas E. DeCoursey
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Article
Biophysics
Christophe Jardin, Gustavo Chaves, Boris Musset
BIOPHYSICAL JOURNAL
(2020)
Article
Biochemistry & Molecular Biology
Gustavo Chaves, Stefanie Bungert-Plumke, Arne Franzen, Iryna Mahorivska, Boris Musset
Article
Physiology
Vladimir V. Cherny, Boris Musset, Deri Morgan, Sarah Thomas, Susan M. E. Smith, Thomas E. DeCoursey
JOURNAL OF GENERAL PHYSIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Gustavo Chaves, Christian Derst, Christophe Jardin, Arne Franzen, Boris Musset
Summary: Voltage-gated proton channels (H(V)1) are expressed in eukaryotes, including insects. Through analysis of Transcriptome Shotgun Assembly (TSA) databases, a natural occurring glutamate variation within the proton selectivity filter of H(V)1 channels was found in Phasmatodea and Mantophasmatodea. Additionally, the expression and function of H(V)1 in insects, specifically in nervous tissue, was studied.
Article
Biochemistry & Molecular Biology
Annika Droste, Gustavo Chaves, Stefan Stein, Annette Trzmiel, Matthias Schweizer, Hubert Karl, Boris Musset
Summary: The respiratory burst of phagocytes relies on the production of reactive oxygen species (ROS) by NADPH oxidase (NOX2) and the voltage-gated proton channel (HV1). The study shows that human PMN and PLB 985 generate ROS through a common mechanism involving NOX2 and HV1. Results suggest an essential interconnection between HV1 and NOX2 during the respiratory burst.
Article
Biochemistry & Molecular Biology
Gustavo Chaves, Artem G. Ayuyan, Vladimir V. Cherny, Deri Morgan, Arne Franzen, Lynne Fieber, Lydia Nausch, Christian Derst, Iryna Mahorivska, Christophe Jardin, Thomas E. DeCoursey, Boris Musset
Summary: Voltage-gated ion channels are divided into subfamilies, with the family of voltage-gated proton channels being very small. In this study, three proton channel genes (AcH(V)1, AcH(V)2, and AcH(V)3) were discovered and characterized in Aplysia californica. These channels exhibited proton selectivity but differed in gating properties. AcH(V)1 was similar to mammalian H-V channels, AcH(V)2 showed extensive inward proton current, and AcH(V)3 displayed proton selective leak currents with weak voltage dependence.
Article
Chemistry, Physical
Christophe Jardin, Niklas Ohlwein, Arne Franzen, Gustavo Chaves, Boris Musset
Summary: The gating of the voltage-gated proton channel H(V)1 is strongly regulated by pH. Experimental investigations and simulations have identified the involvement of titratable amino acids in pH sensing and gating of the channel. Changes in pH cause conformational changes and movement of specific amino acids that ultimately control channel activation.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2022)
Meeting Abstract
Biophysics
Gustavo Chaves, Artem Ayuyan, Vladimir V. Cherny, Deri Morgan, Arne Franzen, Lynne A. Fieber, Lydia Nausch, Christian Derst, Iryna Mahorivska, Christophe Jardin, Thomas E. DeCoursey, Boris Musset
BIOPHYSICAL JOURNAL
(2022)
Meeting Abstract
Biophysics
Danila Boytsov, Stefania Brescia, Christine Siligan, Boris Musset, Peter Pohl
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
(2021)
Meeting Abstract
Biophysics
Richard L. Banh, Vladimir V. Cherny, Deri Morgan, Boris Musset, Sarah Thomas, Kethika Kulleperuma, Susan M. Smith, Thomas E. DeCoursey, Regis Pomes
BIOPHYSICAL JOURNAL
(2020)
Meeting Abstract
Physiology
B. Musset
Meeting Abstract
Physiology
C. Jardin, B. Musset
Meeting Abstract
Physiology
G. Chaves, S. Bungert-Pluemke, A. Arne Franzen, I. Mahorivska, B. Musset