Review
Biochemistry & Molecular Biology
Valerij S. Sokolov, Vladimir V. Cherny, Artem G. Ayuyan, Thomas E. DeCoursey
Summary: H(V)1 channels, resembling the voltage-sensing domain of other voltage-gated ion channels, feature a unique Delta pH dependent gating mechanism that regulates channel opening by pH on both sides of the membrane. Two mechanisms, the countercharge mechanism and the electrostatic mechanism, are proposed to contribute to this Delta pH dependent gating. Both mechanisms are likely to operate together to regulate the gating of H(V)1 channels.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2021)
Article
Multidisciplinary Sciences
Hongbo Chen, Jiahua Deng, Qiang Cui, Baron Chanda, Katherine Henzler-Wildman
Summary: By combining experimental results with molecular dynamics simulations, this study reveals that the engineered temperature-sensitive variant of the Shaker potassium channel has the same overall secondary structure as the wild-type channel, but exhibits local changes in backbone torsion angles near the substitution sites (V369S and F370S). Notably, these structural differences lead to increased hydration of specific regions in the temperature-sensitive variant at higher temperatures, highlighting the impact of subtle differences in primary structure on solvation and temperature-dependent activity.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Ariadna Delgado-Bermudez, Yentel Mateo-Otero, Marc Llavanera, Sergi Bonet, Marc Yeste, Elisabeth Pinart
Summary: General blockade of potassium channels and specific blockade of SLO1 channels did not result in alterations in sperm quality after thawing compared to control samples. In contrast, blocking HVCN1 channels with 2-GBI led to a significant decrease in post-thaw sperm quality compared to the control, despite lower intracellular O-2(-) and H2O2 levels in 2-GBI blocked samples.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Kirin D. Gada, Mengmeng Chang, Aishwarya Chandrashekar, Leigh D. Plant, Sami F. Noujaim, Diomedes E. Logothetis
Summary: G-protein-gated inwardly rectifying potassium (GIRK) channel activity is regulated by phosphatidylinositol-4,5-bisphosphate (PI 4,5P(2)), and PKC epsilon plays a role in the activation of GIRK channels in cardiac atrial cells and human stem cell-derived atrial cardiomyocytes. PKC epsilon enhances the interaction between GIRK4 and GIRK1/4 with PIP2, leading to increased channel activity.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Biochemistry & Molecular Biology
Ariadna Delgado-Bermudez, Marc Yeste, Sergi Bonet, Elisabeth Pinart
Summary: Sperm capacitation requires alkalization of sperm cytosol, with Na+/HCO3- transporters and Cl-/HCO3- exchangers playing key roles in different stages of HCO3- entrance; Proton efflux is crucial for rapid alkalization and activation of proteins in capacitated sperm cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Chemistry, Multidisciplinary
Chen Zhao, Huanting Wang, Huacheng Zhang
Summary: Artificial ion channels mimicking the intelligent gating functions of biological ion channels are crucial for sensing, delivery, and separation. Advances have been made in fabricating bio-inspired switchable ion channels, which show improved gating features and can be smartly regulated using various physical and chemical stimuli.
MATERIALS CHEMISTRY FRONTIERS
(2021)
Article
Multidisciplinary Sciences
Willy Carrasquel-Ursulaez, Ignacio Segura, Ignacio Diaz-Franulic, Felipe Echeverria, Yenisleidy Lorenzo-Ceballos, Nicolas Espinoza, Maximiliano Rojas, Jose Antonio Garate, Eduardo Perozo, Osvaldo Alvarez, Fernando D. Gonzalez-Nilo, Ramon Latorre
Summary: This study demonstrates that two arginines in the transmembrane segment S4 function as the gating charges of BK channels, and the energy landscape of gating particles is tuned by a network of salt bridges in the voltage sensor domain (VSD). Unlike Kv channels, the charge movement in BK seems limited to a small displacement of the guanidinium moieties of R210 and R213.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Rong Shen, Yilin Meng, Benoit Roux, Eduardo Perozo
Summary: This study investigates the conformational changes in voltage-sensing domains (VSDs) during the voltage activation process. Through molecular dynamics simulations and experimental validations, the researchers identified four different conformations visited in the VSD of the phosphatase Ci-VSP. The results provide insights into the energetics and mechanism of voltage activation in VSDs.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Tamer M. Gamal El-Din, Timothy Lantin, Christopher W. Tschumi, Barbara Juarez, Meagan Quinlan, Julia H. Hayano, Jin Li, Larry S. Zweifel, William A. Catterall
Summary: Autism spectrum disorder affects over 1% of children in the United States, resulting in social interaction deficits, repetitive behaviors, and communication disorders. Genetic analysis through genome sequencing has identified over 500 genes with mutations in ASD, suggesting that altered control of action potential generation by gating pore current through mutant K(v)7 channels may be a potential pathogenic mechanism in autism.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Neurosciences
Fan Wang, Xiao-Ru Ma, Yang Wu, Yong-Cheng Xu, Hui-Min Gu, Di-Xian Wang, Zhao-Jun Dong, Hui-Liang Li, Li-Bin Wang, Jing-Wei Zhao
Summary: The study reveals that the expression of HVCN1 in microglia/macrophages is upregulated in various injuries and diseases of the central nervous system, leading to overactivation of HVCN1 and influencing the migration of microglia/macrophages.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Xiuqin Yu, Cuiyan Li, Jianhong Chang, Yujie Wang, Weifeng Xia, Jinquan Suo, Xinyu Guan, Valentin Valtchev, Yushan Yan, Shilun Qiu, Qianrong Fang
Summary: Functionalized covalent organic frameworks (COFs) can serve as an efficient nanofluidic platform for intelligent modulation of ion transport. The materials exhibit outstanding selective ion transmission performance with high gating ratios, among the highest reported in solid-state nanochannels. The research also demonstrates the high tunability, selectivity, and recyclability of ion transport in COFs.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Cell Biology
Edyta Glogowska, Malika Arhatte, Franck C. Chatelain, Florian Lesage, Aimin Xu, Carsten Grashoff, Dennis E. Discher, Amanda Patel, Eric Honore
Summary: The study demonstrates that Piezo1/2 channels can increase the current amplitude of TREK/TRAAK channels, slow down their activation/deactivation rates, and remove their inactivation upon mechanical stimulation. This regulation is mainly achieved through a local depletion of membrane cholesterol, occurring in mouse fibroblasts and affecting wound healing speed.
Article
Biochemistry & Molecular Biology
Daohua Jiang, Richard Banh, Tamer M. Gamal El-Din, Lige Tonggu, Michael J. Lenaeus, Regis Pomes, Ning Zheng, William A. Catterall
Summary: Research has uncovered the open-state structure of the sodium channel Na(v)1.5, revealing the mechanism of pore opening by blocking fast inactivation. This provides crucial molecular-level insights into the initiation of heartbeat.
Article
Physics, Multidisciplinary
Lukasz Machura, Agata Wawrzkiewicz-Jalowiecka, Monika Richter-Laskowska, Paulina Trybek
Summary: The study explores the complexity of empirical data on ionic currents flowing through a single channel in a biological membrane. Simulated data reveal the significant influence of temperature and conformational changes on the complexity of the currents, which are difficult to control experimentally.
Article
Multidisciplinary Sciences
Leandro M. Alonso, Mara C. P. Rue, Eve Marder
Summary: Neurons use intracellular calcium concentrations to regulate their excitability, and models with multiple sensors can distinguish different activity patterns. However, previous models with multiple sensors displayed instabilities leading to unbounded growth of conductances. By introducing a nonlinear degradation term, we prevent conductances from growing beyond a certain bound. The modified model can recover from perturbations and shows that caution must be exercised in interpreting manipulations that mimic increased neuronal activity.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biophysics
Christophe Jardin, Gustavo Chaves, Boris Musset
BIOPHYSICAL JOURNAL
(2020)
Article
Biochemistry & Molecular Biology
Gustavo Chaves, Stefanie Bungert-Plumke, Arne Franzen, Iryna Mahorivska, Boris Musset
Article
Multidisciplinary Sciences
Dan J. Bare, Vladimir V. Cherny, Thomas E. DeCoursey, Abde M. Abukhdeir, Deri Morgan
Article
Physiology
Vladimir V. Cherny, Boris Musset, Deri Morgan, Sarah Thomas, Susan M. E. Smith, Thomas E. DeCoursey
JOURNAL OF GENERAL PHYSIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Gustavo Chaves, Christian Derst, Christophe Jardin, Arne Franzen, Boris Musset
Summary: Voltage-gated proton channels (H(V)1) are expressed in eukaryotes, including insects. Through analysis of Transcriptome Shotgun Assembly (TSA) databases, a natural occurring glutamate variation within the proton selectivity filter of H(V)1 channels was found in Phasmatodea and Mantophasmatodea. Additionally, the expression and function of H(V)1 in insects, specifically in nervous tissue, was studied.
Article
Biochemistry & Molecular Biology
Annika Droste, Gustavo Chaves, Stefan Stein, Annette Trzmiel, Matthias Schweizer, Hubert Karl, Boris Musset
Summary: The respiratory burst of phagocytes relies on the production of reactive oxygen species (ROS) by NADPH oxidase (NOX2) and the voltage-gated proton channel (HV1). The study shows that human PMN and PLB 985 generate ROS through a common mechanism involving NOX2 and HV1. Results suggest an essential interconnection between HV1 and NOX2 during the respiratory burst.
Article
Neurosciences
Jianyong Ma, Xiaoqian Gao, Yutian Li, Thomas E. DeCoursey, Gary E. Shull, Hong-Sheng Wang
Summary: Regulation of intracellular pH is crucial for cardiac function. The HVCN1 proton channel, expressed in canine ventricular myocytes, provides an energetically efficient mechanism for H+ extrusion, contributing to pH regulation and potentially facilitating CO2 disposal.
JOURNAL OF PHYSIOLOGY-LONDON
(2022)
Article
Biochemistry & Molecular Biology
Gustavo Chaves, Artem G. Ayuyan, Vladimir V. Cherny, Deri Morgan, Arne Franzen, Lynne Fieber, Lydia Nausch, Christian Derst, Iryna Mahorivska, Christophe Jardin, Thomas E. DeCoursey, Boris Musset
Summary: Voltage-gated ion channels are divided into subfamilies, with the family of voltage-gated proton channels being very small. In this study, three proton channel genes (AcH(V)1, AcH(V)2, and AcH(V)3) were discovered and characterized in Aplysia californica. These channels exhibited proton selectivity but differed in gating properties. AcH(V)1 was similar to mammalian H-V channels, AcH(V)2 showed extensive inward proton current, and AcH(V)3 displayed proton selective leak currents with weak voltage dependence.
Article
Chemistry, Physical
Christophe Jardin, Niklas Ohlwein, Arne Franzen, Gustavo Chaves, Boris Musset
Summary: The gating of the voltage-gated proton channel H(V)1 is strongly regulated by pH. Experimental investigations and simulations have identified the involvement of titratable amino acids in pH sensing and gating of the channel. Changes in pH cause conformational changes and movement of specific amino acids that ultimately control channel activation.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2022)
Meeting Abstract
Biophysics
Gustavo Chaves, Artem Ayuyan, Vladimir V. Cherny, Deri Morgan, Arne Franzen, Lynne A. Fieber, Lydia Nausch, Christian Derst, Iryna Mahorivska, Christophe Jardin, Thomas E. DeCoursey, Boris Musset
BIOPHYSICAL JOURNAL
(2022)
Meeting Abstract
Biophysics
Danila Boytsov, Stefania Brescia, Christine Siligan, Boris Musset, Peter Pohl
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
(2021)
Meeting Abstract
Biophysics
Richard L. Banh, Vladimir V. Cherny, Deri Morgan, Boris Musset, Sarah Thomas, Kethika Kulleperuma, Susan M. Smith, Thomas E. DeCoursey, Regis Pomes
BIOPHYSICAL JOURNAL
(2020)
Meeting Abstract
Physiology
C. Jardin, B. Musset
Meeting Abstract
Physiology
G. Chaves, S. Bungert-Pluemke, A. Arne Franzen, I. Mahorivska, B. Musset