Article
Biochemistry & Molecular Biology
Qiuxia Chen, You Li, Chunrong Lin, Liu Chen, Hao Luo, Shuai Xia, Chuan Liu, Xuemin Cheng, Chengzhong Liu, Jin Li, Dengfeng Dou
Summary: In this study, DNA-encoded library (DEL) technology was employed for small molecule identification against RNA targets. An optimized selection strategy was developed to minimize false positive signals and demonstrated the feasibility of DEL selection on RNA targets. This study provides a promising opportunity for RNA ligand screening and expands the application of DEL selection in drug discovery.
NUCLEIC ACIDS RESEARCH
(2022)
Review
Biochemistry & Molecular Biology
Clara Woodcraft, Yit-Heng Chooi, Indra Roux
Summary: This review discusses the innovative strategies for fungal natural product discovery and engineering made possible by CRISPR/Cas-based tools, and provides a guide for CRISPR/Cas experiment design. The gaps in genetic tool development for filamentous fungi and the promising opportunities for natural product research are also discussed.
NATURAL PRODUCT REPORTS
(2023)
Review
Biochemistry & Molecular Biology
Laura K. Meredith, Malak M. Tfaily
Summary: Microbial metabolomic studies have focused mainly on nonvolatile metabolites, neglecting the volatile organic compounds (VOCs) that play important roles in microbial systems. Advances in VOC detection allow real-time observation and overcome sampling limitations, leading to a more comprehensive understanding of microbial processes when integrating VOC and other 'omics approaches.
TRENDS IN MICROBIOLOGY
(2022)
Article
Plant Sciences
Naoki Wada, Keishi Osakabe, Yuriko Osakabe
Summary: Recently discovered and characterized CRISPR-Cas systems provide new tools for plant genome editing. Several new CRISPR-Cas systems have been applied successfully to plant and human genome editing, offering higher specificity and unique characteristics compared to known Cas proteins.
Article
Biochemistry & Molecular Biology
Sharon Moshitch-Moshkovitz, Dan Dominissini, Gideon Rechavi
Summary: In the last decade, thousands of studies have demonstrated the involvement of mRNA modifications in the regulation of gene expression. New technologies and methods have enabled accurate identification, transcriptome-wide mapping, and functional characterization of a growing number of RNA modifications, providing important insights into their biological role.
Review
Chemistry, Multidisciplinary
Sergio Gonzalez-Granda, Jesus Albarran-Velo, Ivan Lavandera, Vicente Gotor-Fernandez
Summary: The combination of metal-, photo-, enzyme-, and/or organocatalysis is a versatile approach in synthetic chemistry, particularly for the creation of chiral centers. This review focuses on the recent developments in the simultaneous use of enzymes and transition metal catalysts, as well as the integration of multiple bioprocesses and metal-catalyzed transformations in linear cascades. Challenges such as catalyst compatibility, reaction media, and cross-reactivities are addressed by strategies such as catalyst coimmobilization, compartmentalization, and flow chemistry. The review also highlights the application of bionanohybrid materials, where enzymes and metals are coimmobilized on suitable supports.
Article
Optics
J. E. Castro, T. J. Steiner, L. Thiel, A. Dinkelacker, C. McDonald, P. Pintus, L. Chang, J. E. Bowers, G. Moody
Summary: Aluminum gallium arsenide-on-insulator (AlGaAsOI) is a promising platform for integrated quantum photonics, with large optical nonlinearities, wide tunable bandgap, low propagation loss, and a large thermo-optic coefficient. We have demonstrated edge couplers, splitters, interferometers, and waveguide crossings in AlGaAsOI with performance comparable to or exceeding other quantum photonic platforms.
Review
Biochemistry & Molecular Biology
Guanwen Liu, Qiupeng Lin, Shuai Jin, Caixia Gao
Summary: The emergence of CRISPR-Cas systems has greatly advanced gene editing technologies in the field of life sciences. Various CRISPR-Cas tools have been engineered to improve their performance, allowing for a broader range of targets, higher efficiency and specificity, and greater precision. Additionally, functional elements have been introduced to enable precise gene modification, gene expression control, and epigenetic changes. This review provides an overview of different types of CRISPR-Cas tools, discusses current limitations, and explores future prospects for optimizing CRISPR-Cas systems.
Article
Engineering, Biomedical
Qinqin Cheng, Zhefu Dai, Xiaojing Shi, Xinping Duan, Yiling Wang, Tianling Hou, Yong Zhang
Summary: Exosomes, as cell-derived extracellular vesicles, are crucial for intercellular communication and have emerged as promising nanocarriers for therapeutic applications. By genetically engineering exosomes, a new type of exosomes called GIFTed-Exos with specific functions can be created to efficiently deliver different types of protein cargos to target cells.
Article
Pharmacology & Pharmacy
Kiran S. Toti, Nicole Pribut, Michael D'Erasmo, Madhuri Dasari, Savita K. Sharma, Perry W. Bartsch, Samantha L. Burton, Hannah B. Gold, Anatoliy Bushnev, Cynthia A. Derdeyn, Adriaan E. Basson, Dennis C. Liotta, Eric J. Miller
Summary: Nucleoside- and nucleotide-based therapeutics play crucial roles in treating malignant and viral diseases. Prodrugs, commonly used for improving bioavailability and efficacy, can be optimized with small adjustments to enhance pharmacokinetic profiles. In this study, the fine-tuning of lipid prodrugs of tenofovir, a nucleotide HIV reverse transcriptase inhibitor, successfully reduced liver omega-oxidation while maintaining potent antiviral activity. This research provides important insights into lipid prodrug strategies and the delivery of acyclic nucleoside phosphonates.
FRONTIERS IN PHARMACOLOGY
(2023)
Review
Immunology
David Y. Oh, Lawrence Fong
Summary: Cytotoxic CD4(+) T cells play important roles in both cancer and non-cancer environments, where they can directly kill cancer cells. They are also found in situations like infection and autoimmunity. The cytolytic mechanisms of cytotoxic CD4(+) T cells vary across different disease states.
Review
Chemistry, Multidisciplinary
Oskar G. Wood, Chris S. Hawes
Summary: This article summarizes the important role of azolate-containing ligands in the development of water-stable metal-organic frameworks and related materials, and explores the potential of fused ring analogues as anionic bridging ligands. The article introduces two design approaches and highlights the key features that the ideal compact and stable heterocyclic linker in functional metal-organic frameworks may possess.
Review
Biotechnology & Applied Microbiology
Chunxiao Yan, Wei Yu, Lun Yao, Xiaoyu Guo, Yongjin J. Zhou, Jiaoqi Gao
Summary: This review summarizes the characterization and engineering of promoters in methylotrophic yeasts. It discusses the applications of constitutive and inducible promoters controlled by methanol or other inducers/repressors in protein and chemical production. It also highlights efforts in promoter engineering, such as site-directed mutagenesis, hybrid promoter, and transcription factor regulation, to expand the promoter toolbox.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Endocrinology & Metabolism
Thomas L. L. Williams, Robyn G. C. Macrae, Rhoda E. E. Kuc, Alastair J. H. Brown, Janet J. J. Maguire, Anthony P. P. Davenport
Summary: This article describes the design and validation of four novel fluorescent ligands that can bind to the apelin receptor and trigger receptor internalization. These ligands have potential applications in regulating the human cardiovascular system and drug discovery.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Ecology
Franziska Komossa, Flurina M. Wartmann, Peter H. Verburg
Summary: The study analyzed how different data gathering methods can support local recreation planning and found that local planners have limited knowledge of and access to scientific methods. Some potential methods, such as free listing or participatory mapping, are not yet applied for local planning. The potential use of social media data is appealing, but there is currently a lack of necessary skills for collecting and analyzing data.
LANDSCAPE AND URBAN PLANNING
(2021)
Article
Biotechnology & Applied Microbiology
Leander Meuris, Francis Santens, Greg Elson, Nele Festjens, Morgane Boone, Anaelle Dos Santos, Simon Devos, Francois Rousseau, Evelyn Plets, Erica Houthuys, Pauline Malinge, Giovanni Magistrelli, Laura Cons, Laurence Chatel, Bart Devreese, Nico Callewaert
NATURE BIOTECHNOLOGY
(2014)
Meeting Abstract
Biochemical Research Methods
Morgane Boone, Yao-Cheng Lin, Leander Meuris, Irma Lemmens, Nadine Van Roy, Arne Soete, Joke Reumers, Matthieu Moisse, Stephane Plaisance, Radoje Drmanac, Jason Chen, Frank Speleman, Diether Lambrechts, Yves Van de Peer, Jan Tavernier, Nico Callewaert
Article
Multidisciplinary Sciences
Yao-Cheng Lin, Morgane Boone, Leander Meuris, Irma Lemmens, Nadine Van Roy, Arne Soete, Joke Reumers, Matthieu Moisse, Stephane Plaisance, Radoje Drmanac, Jason Chen, Frank Speleman, Diether Lambrechts, Yves Van de Peer, Jan Tavernier, Nico Callewaert
NATURE COMMUNICATIONS
(2014)
Article
Biology
Karina Perlaza, Hannah Toutkoushian, Morgane Boone, Mable Lam, Masakazu Iwai, Martin C. Jonikas, Peter Walter, Silvia Ramundo
Article
Multidisciplinary Sciences
Silvia Ramundo, Yukari Asakura, Patrice A. Salome, Daniela Strenkert, Morgane Boone, Luke C. M. Mackinder, Kazuaki Takafuji, Emine Dinc, Michele Rahire, Michele Crevecoeur, Leonardo Magneschi, Olivier Schaad, Michael Hippler, Martin C. Jonikas, Sabeeha Merchant, Masato Nakai, Jean-David Rochaix, Peter Walter
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Biology
Karen Krukowski, Amber Nolan, Elma S. Frias, Morgane Boone, Gonzalo Ureta, Katherine Grue, Maria-Serena Paladini, Edward Elizarraras, Luz Delgado, Sebastian Bernales, Peter Walter, Susanna Rosi
Article
Biology
Michael Schoof, Morgane Boone, Lan Wang, Rosalie Lawrence, Adam Frost, Peter Walter
Summary: The integrated stress response (ISR) is activated by phosphorylation of translation initiation factor eIF2 in response to various stress conditions. Phosphorylated eIF2 inhibits eIF2B, leading to ISR induction. ISRIB can inhibit ISR even in fully assembled eIF2B decamers, suggesting allosteric communication between distant binding sites.
Article
Multidisciplinary Sciences
Michael Schoof, Lan Wang, J. Zachery Cogan, Rosalie E. Lawrence, Morgane Boone, Jennifer Deborah Wuerth, Adam Frost, Peter Walter
Summary: Viral infection activates the integrated stress response by phosphorylating eIF2, which inhibits eIF2B. The NSs protein of Sandfly Fever Sicilian virus allows the virus to evade the ISR by blocking eIF2-P binding to eIF2B.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Morgane Boone, Pathmanaban Ramasamy, Jasper Zuallaert, Robbin Bouwmeester, Berre Van Moer, Davy Maddelein, Demet Turan, Niels Hulstaert, Hannah Eeckhaut, Elien Vandermarliere, Lennart Martens, Sven Degroeve, Wim De Neve, Wim Vranken, Nico Callewaert
Summary: SECRiFY method is developed to assess the secretability of protein fragments, revealing the importance of intrinsic disorder and chain flexibility in protein features. The data generated by SECRiFY enables advanced machine learning methods to deduce patterns of secretability in protein sequences.
NATURE COMMUNICATIONS
(2021)
Article
Biology
Morgane Boone, Lan Wang, Rosalie E. Lawrence, Adam Frost, Peter Walter, Michael Schoof
Summary: In eukaryotic cells, stressors activate the integrated stress response (ISR), which reprograms the cellular proteome. Translational control is mainly achieved through the conformational switch of eIF2B, the nucleotide exchange factor of eIF2, resulting in eIF2-P independent activation of ISR.
Article
Multidisciplinary Sciences
Michael Schoof, Bryan Faust, Reuben A. Saunders, Smriti Sangwan, Veronica Rezelj, Nick Hoppe, Morgane Boone, Christian B. Billesbolle, Cristina Puchades, Caleigh M. Azumaya, Huong T. Kratochvil, Marcell Zimanyi, Ishan Deshpande, Jiahao Liang, Sasha Dickinson, Henry C. Nguyen, Cynthia M. Chio, Gregory E. Merz, Michael C. Thompson, Devan Diwanji, Kaitlin Schaefer, Aditya A. Anand, Niv Dobzinski, Beth Shoshana Zha, Camille R. Simoneau, Kristoffer Leon, Kris M. White, Un Seng Chio, Meghna Gupta, Mingliang Jin, Fei Li, Yanxin Liu, Kaihua Zhang, David Bulkley, Ming Sun, Amber M. Smith, Alexandrea N. Rizo, Frank Moss, Axel F. Brilot, Sergei Pourmal, Raphael Trenker, Thomas Pospiech, Sayan Gupta, Benjamin Barsi-Rhyne, Vladislav Belyy, Andrew W. Barile-Hill, Silke Nock, Yuwei Liu, Nevan J. Krogan, Corie Y. Ralston, Danielle L. Swaney, Adolfo Garcia-Sastre, Melanie Ott, Marco Vignuzzi, Peter Walter, Aashish Manglik
