Missed cleavage opportunities by FEN1 lead to Okazaki fragment maturation via the long-flap pathway

Direct Visualization of RNA-DNA Primer Removal from Okazaki Fragments Provides Support for Flap Cleavage and Exonucleolytic Pathways in Eukaryotic Cells

(2017) Bochao Liu et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Interplay of catalysis, fidelity, threading, and processivity in the exo- and endonucleolytic reactions of human exonuclease I

(2017) Yuqian Shi et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Phosphate steering by Flap Endonuclease 1 promotes 5′-flap specificity and incision to prevent genome instability

(2017) Susan E. Tsutakawa et al.   Nature Communications

Single-molecule FRET unveils induced-fit mechanism for substrate selectivity in flap endonuclease 1

(2017) Fahad Rashid et al.   eLife

Resolving individual steps of Okazaki-fragment maturation at a millisecond timescale

(2016) Joseph L Stodola et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Global analysis of genomic instability caused by DNA replication stress inSaccharomyces cerevisiae

(2016) Dao-Qiong Zheng et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Single-molecule imaging reveals the mechanism of Exo1 regulation by single-stranded DNA binding proteins

(2016) Logan R. Myler et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Resolving individual steps of Okazaki-fragment maturation at a millisecond timescale

(2016) Joseph L Stodola et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Rate-determining Step of Flap Endonuclease 1 (FEN1) Reflects a Kinetic Bias against Long Flaps and Trinucleotide Repeat Sequences

(2015) Mary E. Tarantino et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Dna2 nuclease-helicase structure, mechanism and regulation by Rpa

(2015) Chun Zhou et al.   eLife

The balancing act of DNA repeat expansions

(2013) Jane C Kim et al.   CURRENT OPINION IN GENETICS & DEVELOPMENT

Single-molecule characterization of Fen1 and Fen1/PCNA complexes acting on flap substrates

(2013) Timothy D. Craggs et al.   NUCLEIC ACIDS RESEARCH

Human Fanconi Anemia Complementation Group A Protein Stimulates the 5’ Flap Endonuclease Activity of FEN1

(2013) Liangyue Qian et al.   PLoS One

Okazaki Fragment Metabolism

(2013) L. Balakrishnan et al.   Cold Spring Harbor Perspectives in Biology

Sequential and Multistep Substrate Interrogation Provides the Scaffold for Specificity in Human Flap Endonuclease 1

(2013) Mohamed A. Sobhy et al.   Cell Reports

Structure and conformational change of a replication protein A heterotrimer bound to ssDNA

(2012) J. Fan et al.   GENES & DEVELOPMENT

Biochemical analyses indicate that binding and cleavage specificities define the ordered processing of human Okazaki fragments by Dna2 and FEN1

(2012) Jason W. Gloor et al.   NUCLEIC ACIDS RESEARCH

Flap endonucleases pass 5′-flaps through a flexible arch using a disorder-thread-order mechanism to confer specificity for free 5′-ends

(2012) Nikesh Patel et al.   NUCLEIC ACIDS RESEARCH

Human Flap Endonuclease Structures, DNA Double-Base Flipping, and a Unified Understanding of the FEN1 Superfamily

(2011) Susan E. Tsutakawa et al.   CELL

Versatile single-molecule multi-color excitation and detection fluorescence setup for studying biomolecular dynamics

(2011) M. A. Sobhy et al.   REVIEW OF SCIENTIFIC INSTRUMENTS

Okazaki fragment maturation: nucleases take centre stage

(2011) L. Zheng et al.   Journal of Molecular Cell Biology

Defining the Limits of Single-Molecule FRET Resolution in TIRF Microscopy

(2010) Seamus J. Holden et al.   BIOPHYSICAL JOURNAL

Components of the Secondary Pathway Stimulate the Primary Pathway of Eukaryotic Okazaki Fragment Processing

(2010) Ryan A. Henry et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Maintaining genome stability at the replication fork

(2010) Dana Branzei et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Learning Rates and States from Biophysical Time Series: A Bayesian Approach to Model Selection and Single-Molecule FRET Data

(2009) Jonathan E. Bronson et al.   BIOPHYSICAL JOURNAL

Pif1 Helicase Lengthens Some Okazaki Fragment Flaps Necessitating Dna2 Nuclease/Helicase Action in the Two-nuclease Processing Pathway

(2009) Jason E. Pike et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Significance of the Dissociation of Dna2 by Flap Endonuclease 1 to Okazaki Fragment Processing inSaccharomyces cerevisiae

(2009) Jason A. Stewart et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

The 3′-Flap Pocket of Human Flap Endonuclease 1 Is Critical for Substrate Binding and Catalysis

(2009) L. David Finger et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Acetylation of Dna2 Endonuclease/Helicase and Flap Endonuclease 1 by p300 Promotes DNA Stability by Creating Long Flap Intermediates

(2009) Lata Balakrishnan et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Lysine Acetylation Targets Protein Complexes and Co-Regulates Major Cellular Functions

(2009) C. Choudhary et al.   SCIENCE

Polymerase Dynamics at the Eukaryotic DNA Replication Fork

(2008) Peter M. J. Burgers   JOURNAL OF BIOLOGICAL CHEMISTRY

Flexibility of Eukaryotic Okazaki Fragment Maturation through Regulated Strand Displacement Synthesis

(2008) Carrie M. Stith et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

An Oxygen Scavenging System for Improvement of Dye Stability in Single-Molecule Fluorescence Experiments

(2007) Colin Echeverría Aitken et al.   BIOPHYSICAL JOURNAL