Article
Biochemistry & Molecular Biology
Weisheng Zhu, Luyao Wang, Qisi Li, Lizhi Jiao, Xiaokan Yu, Xiangfan Gao, Hao Qiu, Zhijun Zhang, Wei Bing
Summary: Nanozymes, as nanostructures with enzyme-like activity, have gained extensive attention for their potential applications in bacterial disinfection, particularly in promoting ROS generation to kill bacteria. Cerium oxide nanoparticles, a classical nanozyme with high peroxidase activity, have been highlighted as efficient candidates for building bacterial disinfection systems. The CeO2 nanozymes not only promote ROS production but also possess activities for ROS scavenging, raising the question of which activity dominates in the CeO2-H2O2 system and its impact on antibacterial effects.
Article
Environmental Sciences
Yu-Juan Lin, Xing-Hui Feng, Yu-Xi Feng
Summary: This study evaluated the importance of hydrogen sulfide (NaHS) and nitric oxide (SNP) in the antioxidant systems of rice seedlings under Cr(III) stress. The results showed that NaHS and SNP effectively alleviated the toxic effects of Cr(III) by activating different antioxidants. NaHS had a stronger regulatory effect on non-enzymatic and enzymatic antioxidants compared to SNP.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Chemistry, Multidisciplinary
Jie Yu, Ruili Zhang, Baihang Chen, Xuelan Liu, Qian Jia, Xiaofei Wang, Zuo Yang, Pengbo Ning, Zhongliang Wang, Yang Yang
Summary: This study establishes a system consisting of a degradable hydrogel dressing that reacts to reactive oxygen species, an encapsulated biosafe nitric oxide donor, and a low concentration of hydrogen peroxide as a disinfectant. This system promotes sustainable wound healing and disinfection by generating nitric oxide and mediating cell migration and collagen synthesis. It also demonstrates synergistic antibacterial properties against antibiotic-resistant bacteria.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Review
Plant Sciences
Adil Hussain, Farooq Shah, Farman Ali, Byung-Wook Yun
Summary: Senescence is the final stage of plant growth and development, involving significant changes at the transcriptional, post-translational, and metabolomic levels. Plant hormones and nitric oxide play crucial roles in aging and death processes.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Ziwei Liu, Feiqiang He, Limin Zhou, Zhuyao Li, Lirong Zhong, Jianhua Ding
Summary: In this study, Fe3O4-magnetic microspheres were used as the matrix with loaded FeEDTA to prepare magnetic denitrification absorbent Fe3O4@FeEDTA. The results showed that Fe3O4@FeEDTA could effectively remove NO, especially in the presence of NaOH or NaOH-H2O2 system. The NO removal mechanism was preliminarily analyzed through X-ray photoelectron spectroscopy and electron spin resonance.
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2023)
Article
Fisheries
Si Yao Zheng, Xinbin Shao, Zhitao Qi, Maocang Yan, Min Hui Tao, Xiao Man Wu, Lining Zhang, Jianzhong Ma, An Li, Ming Xian Chang
Summary: This study firstly demonstrates the functional characterization of zebrafish nos2a during bacterial infection, revealing its regulation of ROS and NO as well as the expression of proinflammatory cytokines, antibacterial genes, and oxidant factors. Furthermore, it finds that inhibition of NO can be effective in protecting against E. piscicida infection.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Plant Sciences
Lu Lu, Xinru Wu, Pengkai Wang, Liming Zhu, Yuxin Liu, Yao Tang, Zhaodong Hao, Ye Lu, Jingbo Zhang, Jisen Shi, Tielong Cheng, Jinhui Chen
Summary: NbCIPK25, identified in the halophyte Nitraria billardieri, is a salt-responsive gene that can enhance salt tolerance when overexpressed in transgenic plants. By mediating the scavenging of reactive oxygen species, NbCIPK25 protects plant cells from oxidation and maintains normal development under salt stress.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Chemistry, Analytical
Mei-Jia Hou, Zhi-Qing Wang, Jun-Tao Chen, Zhi-Ke Tan, Guo-Jiang Mao, Junjie Fei, Chun-Yan Li
Summary: This study developed a fluorescent nanoprobe for sequential detection of ATP and ONOO-, showing potential for accurate cancer diagnosis by distinguishing cancer cells from normal cells.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Yuting Shi, Yufei Cao, Ju Cheng, Wenwen Yu, Mingsheng Liu, Juanjuan Yin, Congshu Huang, Xiaoqin Liang, Haicun Zhou, Hongbin Liu, Zheng Yang, Yu Fang, Hua Wei, Guanghui Zhao
Summary: The traditional treatment for bacterial infections is the use of antibiotics, which can lead to the formation of multidrug-resistant bacteria and biofilms. A new approach using amphiphilic supramolecules has been developed to address this issue and successfully applied in the construction of nanocarriers.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Plant Sciences
Yaoqi Li, Qiong Nan, Yinai Liu, Sue Lin, Hao Ji, Da Sun, Libo Jin, Renyi Peng
Summary: The study found that pretreatment with the NO donor SNP enhanced hypoxia tolerance in maize seedlings by increasing Ca2+ concentration and ADH activities in the roots. It also resulted in a higher antioxidant system capacity and upregulation of hypoxia-responsive gene expression. Overall, NO was found to regulate the response of maize roots to hypoxia.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Review
Biochemistry & Molecular Biology
Chiara Pucciariello, Pierdomenico Perata
Summary: This review summarizes recent findings on the roles of ROS and NO under environmentally or developmentally defined low O-2 conditions. Research indicates that ROS and NO are emerging regulators in low O-2 signaling and crucial molecules in plant adaptation to flooding conditions.
Article
Plant Sciences
Weronika Skowronska, Sebastian Granica, Monika E. Czerwinska, Ewa Osinska, Agnieszka Bazylko
Summary: The study confirms the anti-inflammatory and antioxidant activity of elderberry leaves, particularly against reactive oxygen species. This suggests that elderberry leaves may be a potential medicinal plant with anti-inflammatory and antioxidant properties.
JOURNAL OF ETHNOPHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Pietro Barbacini, Enrica Torretta, Beatrice Arosio, Evelyn Ferri, Daniele Capitanio, Manuela Moriggi, Cecilia Gelfi
Summary: Sphingolipids play an important role in aging and various disorders. This study analyzed the distribution of sphingolipid species and enzyme levels associated with centenarians' decelerated aging. The results showed that centenarians had a specific profile of sphingolipids and a fine-tuning mechanism, indicating a protective mechanism against physiological decline.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Diksha Kumari, Kuljit Singh
Summary: Leishmaniasis, a neglected tropical disease caused by different species of Leishmania parasite, still poses a global concern. This review focuses on the immunological perspectives of the disease, discussing the role of host immune cells in countering parasite infection and highlighting recent discoveries in leishmanicidal molecules/compounds. The knowledge gained from this review can contribute to the identification of biomarkers, novel therapeutics, and vaccine candidates against leishmaniasis.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Article
Chemistry, Analytical
Yang Tian, Senyao Liu, Wenwen Cao, Peng Wu, Zhaoming Chen, Hu Xiong
Summary: In this study, an activatable near-infrared II (NIR-II) fluorescent probe (IR-990) was developed for real-time detection of endogenous H2O2 in the progression of drug-induced liver injury (DILI). The probe exhibited intense NIR-II fluorescence in the presence of H2O2, and showed high sensitivity and selectivity in vitro and in vivo experiments.
ANALYTICAL CHEMISTRY
(2022)
Review
Plant Sciences
Nishat Parveen, Nidhi Kandhol, Shivesh Sharma, Vijay Pratap Singh, Devendra Kumar Chauhan, Jutta Ludwig-Mueller, Francisco J. Corpas, Durgesh Kumar Tripathi
Summary: The plant hormone auxin plays a crucial role in regulating plant growth and development. Reactive oxygen species (ROS) also serve as signaling molecules at low concentrations, but excessive accumulation of ROS due to environmental stresses can be detrimental to cells. Nitric oxide (NO), a gaseous signaling molecule, has various beneficial effects on plants. Together, auxin, ROS, and NO are involved in developmental processes and abiotic stress tolerance in plants.
PLANT AND CELL PHYSIOLOGY
(2023)
Editorial Material
Biochemistry & Molecular Biology
Luciano Freschi, Francisco J. Corpas, Jose M. Palma
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Salvador Gonzalez-Gordo, Jose M. Palma, Francisco J. Corpas
Summary: Small heat shock proteins (sHSPs) are upregulated in plants under environmental stresses and act as molecular chaperones to protect other proteins. This study identified 41 sHSP genes in the genome of sweet pepper fruits, with 19 genes found in previous transcriptome data. The expression analysis during fruit ripening showed that 6 genes were upregulated, 7 genes were downregulated, and 6 genes were unchanged. In addition, nitric oxide treatment caused the upregulation of 7 genes, downregulation of 3 genes, and no change in 9 genes. These findings indicate the role of sHSPs in pepper fruit ripening and nitro-oxidative stress.
Article
Biochemistry & Molecular Biology
Maria A. Munoz-Vargas, Javier Lopez-Jaramillo, Salvador Gonzalez-Gordo, Alberto Paradela, Jose M. Palma, Francisco J. Corpas
Summary: This study aims to analyze the interrelationship between nitric oxide (NO) and hydrogen sulfide (H2S) during pepper fruit ripening. The results show that the activity of H2S-generating enzymes LCD and DCD is downregulated during ripening, but it can be reversed by NO treatment. These findings enhance our understanding of the relationship between NO and H2S during pepper fruit ripening.
ANTIOXIDANTS & REDOX SIGNALING
(2023)
Meeting Abstract
Biochemistry & Molecular Biology
Marta Rodriguez-Ruiz, Carmen Ramos, Maria Jesus Campos, Francisca Vicente, Francisco J. Corpas, Jose M. Palma
FREE RADICAL BIOLOGY AND MEDICINE
(2023)
Meeting Abstract
Biochemistry & Molecular Biology
Jorge Taboada, Salvador Gonzalez-Gordo, Jose M. Palma, Francisco J. Corpas
FREE RADICAL BIOLOGY AND MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Salvador Gonzalez-Gordo, Javier Lopez-Jaramillo, Jose M. Palma, Francisco J. Corpas
Summary: Hydrogen sulfide (H2S) and nitric oxide (NO) negatively affect the activity of soybean lipoxygenase type 1 (LOX 1), potentially through S-nitrosation and persulfidation. Among the thirty-five tyrosine residues present in LOX 1, only Y214 was exclusively nitrated by peroxynitrite (ONOO-), thereby affecting its interaction with W500 in the substrate binding site. Analysis of the protein structure reveals the existence of tunnels connecting the protein surface with internal cysteine residues, facilitating persulfidation (especially C429 and C127) and nitrosation (particularly of C127) by H2S and the hydrophilic, bulkier CysNO molecule, respectively.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Editorial Material
Biochemistry & Molecular Biology
Weibiao Liao, Abir U. Igamberdiev, Jose M. Palma
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Salvador Gonzalez-Gordo, Maria A. Munoz-Vargas, Jose M. Palma, Francisco J. Corpas
Summary: The study identified 75 CaPOD genes in sweet pepper based on the existing genome, but only 10 genes were found to be active in the fruit transcriptome. Two genes were upregulated during fruit ripening, seven were downregulated, and one gene was unaffected. Nitric oxide treatment triggered the upregulation of two CaPOD genes while the others were unaffected. In vitro experiments showed that CaPOD IV activity was strongly inhibited by peroxynitrite, NO donors, and reducing agents.
Article
Plant Sciences
Jorge Taboada, Salvador Gonzalez-Gordo, Maria A. Munoz-Vargas, Jose M. Palma, Francisco J. Corpas
Summary: NADPH is an essential cofactor involved in various physiological processes. In pepper fruits, five NADP-ME genes were identified and four of them were found to be expressed. The expression of these genes was differentially modulated during fruit ripening, with CaNADP-ME3 and CaNADP-ME5 upregulated, and CaNADP-ME2 and CaNADP-ME4 downregulated. Exogenous NO gas treatment triggered the downregulation of CaNADP-ME4. Protein fraction containing CaNADP-ME enzyme activity was obtained and four isozymes were identified.
Article
Biochemistry & Molecular Biology
Marta Rodriguez-Ruiz, Maria C. Ramos, Maria J. Campos, Inmaculada Diaz-Sanchez, Bastien Cautain, Thomas A. A. Mackenzie, Francisca Vicente, Francisco J. J. Corpas, Jose M. Palma
Summary: Cancer, characterized by an alteration of the oxidative metabolism, is a major cause of human death worldwide. Many natural compounds from plant origin with anti-tumor attributes have been described, including capsaicin, the molecule responsible for the pungency in hot pepper fruits, which has shown anti-proliferative properties against cancer. The study found that the capsaicin content in pepper fruits did not correspond with their anti-proliferative activity, indicating the presence of other compounds with anti-tumor potentiality in pepper fruits.
Article
Plant Sciences
Maria A. Munoz-Vargas, Salvador Gonzalez-Gordo, Jorge Taboada, Jose M. Palma, Francisco J. Corpas
Summary: This study investigated the transcriptomic, molecular, and functional levels of the G6PDH system in pepper fruit during ripening. The results showed that different CaG6PDH genes were differentially regulated during different ripening stages. Exogenous treatment with NO gas downregulated CaG6PDH2, while positively regulating other genes. In-gel analysis identified two isozymes, CaG6PDH I and CaG6PDH II. The study provides insights into the modulation of CaG6PDHs at the gene and activity levels in pepper fruit during ripening and after NO post-harvest treatment.
Article
Biochemistry & Molecular Biology
Julia Claudiane da Veiga, Neidiquele Maria Silveira, Amedea Barozzi Seabra, Ilana Urbano Bron
Summary: This review investigates the strategy of using nitric oxide (NO) donors to extend postharvest shelf-life and improve fruit quality, discusses the interactions between NO and ethylene or reactive oxygen species, and highlights the potential of nanotechnology in NO applications.
NITRIC OXIDE-BIOLOGY AND CHEMISTRY
(2024)