Construction of Self-Activated Nanoreactors for Cascade Catalytic Anti-Biofilm Therapy Based on H2O2 Self-Generation and Switch-On NO Release

The traditional treatment for bacterial infections is the use of antibiotics, but the overuse of antibiotics can lead to the formation of multidrug-resistant bacteria, and eventually lead to the formation of biofilms, which can resist the attack of the host immune system and the penetration of antibiotics in biofilms. Although biofilm-targeted and stimulus-responsive antibiotic delivery nanocarriers are available, the drugs that can be delivered is extremely limited. Therefore, an amphiphilic supramolecule Arg-CD-AcMH is constructed via host-guest interactions between beta-CD-terminated arginine (Arg-CD) and ferrocene-terminated acetal-modified maltoheptaose. The amphiphilic supramolecule Arg-CD-AcMH can self-assemble with glucoamylase and glucose oxidase in aqueous solution to form spherical nanoparticles through hydrophilic-hydrophobic and electrostatic interactions. These nanoparticles can respond to the micro-environment in the biofilm and selectively increase the level of H2O2 in the biofilm through the cascading catalytic effect of enzymes based on the self-supply glucose, thus providing conditions for the release of anti-biofilm active drug nitric oxide.

Automated summary

The traditional treatment for bacterial infections is the use of antibiotics, which can lead to the formation of multidrug-resistant bacteria and biofilms. A new approach using amphiphilic supramolecules has been developed to address this issue and successfully applied in the construction of nanocarriers.