Colonoscopy-based colorectal cancer modeling in mice with CRISPR–Cas9 genome editing and organoid transplantation

OncogenicKrasdrives invasion and maintains metastases in colorectal cancer

(2017) Adam T. Boutin et al.   GENES & DEVELOPMENT

A distinct role for Lgr5+ stem cells in primary and metastatic colon cancer

(2017) Felipe de Sousa e Melo et al.   NATURE

A Wnt-producing niche drives proliferative potential and progression in lung adenocarcinoma

(2017) Tuomas Tammela et al.   NATURE

In vivo genome editing and organoid transplantation models of colorectal cancer and metastasis

(2017) Jatin Roper et al.   NATURE BIOTECHNOLOGY

Transplantation of engineered organoids enables rapid generation of metastatic mouse models of colorectal cancer

(2017) Kevin P O'Rourke et al.   NATURE BIOTECHNOLOGY

Genetic dissection of colorectal cancer progression by orthotopic transplantation of engineered cancer organoids

(2017) Arianna Fumagalli et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Iron Uptake via DMT1 Integrates Cell Cycle with JAK-STAT3 Signaling to Promote Colorectal Tumorigenesis

(2016) Xiang Xue et al.   Cell Metabolism

A Colorectal Tumor Organoid Library Demonstrates Progressive Loss of Niche Factor Requirements during Tumorigenesis

(2016) Masayuki Fujii et al.   Cell Stem Cell

Endoscopy-guided orthotopic implantation of colorectal cancer cells results in metastatic colorectal cancer in mice

(2016) Dominik Bettenworth et al.   CLINICAL & EXPERIMENTAL METASTASIS

High-fat diet enhances stemness and tumorigenicity of intestinal progenitors

(2016) Semir Beyaz et al.   NATURE

Generation of an inducible colon-specific Cre enzyme mouse line for colon cancer research

(2016) Paul W. Tetteh et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Modular Assembly Platform for Rapid Generation of DNA Constructs

(2016) Elliot H. Akama-Garren et al.   Scientific Reports

Colon Tumors with the Simultaneous Induction of Driver Mutations in APC, KRAS, and PIK3CA Still Progress through the Adenoma-to-carcinoma Sequence

(2015) J. N. Hadac et al.   Cancer Prevention Research

Apc Restoration Promotes Cellular Differentiation and Reestablishes Crypt Homeostasis in Colorectal Cancer

(2015) Lukas E. Dow et al.   CELL

Prospective Derivation of a Living Organoid Biobank of Colorectal Cancer Patients

(2015) Marc van de Wetering et al.   CELL

Colorectal cancer models for novel drug discovery

(2015) Daniel Golovko et al.   Expert Opinion on Drug Discovery

Sequential cancer mutations in cultured human intestinal stem cells

(2015) Jarno Drost et al.   NATURE

Modeling colorectal cancer using CRISPR-Cas9–mediated engineering of human intestinal organoids

(2015) Mami Matano et al.   NATURE MEDICINE

Efficient genetic engineering of human intestinal organoids using electroporation

(2015) Masayuki Fujii et al.   Nature Protocols

Combination PI3K/MEK inhibition promotes tumor apoptosis and regression in PIK3CA wild-type, KRAS mutant colorectal cancer

(2014) Jatin Roper et al.   CANCER LETTERS

CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling

(2014) Randall J. Platt et al.   CELL

Rapid modelling of cooperating genetic events in cancer through somatic genome editing

(2014) Francisco J. Sánchez-Rivera et al.   NATURE

Lymph node-independent liver metastasis in a model of metastatic colorectal cancer

(2014) Ida B. Enquist et al.   Nature Communications

Functional Repair of CFTR by CRISPR/Cas9 in Intestinal Stem Cell Organoids of Cystic Fibrosis Patients

(2013) Gerald Schwank et al.   Cell Stem Cell

Development of a Colon Cancer GEMM-Derived Orthotopic Transplant Model for Drug Discovery and Validation

(2013) E. S. Martin et al.   CLINICAL CANCER RESEARCH

In vitro expansion and genetic modification of gastrointestinal stem cells in spheroid culture

(2013) Hiroyuki Miyoshi et al.   Nature Protocols

Fluorescence endoscopic detection of murine colitis-associated colon cancer by topically applied enzymatically rapid-activatable probe

(2012) Makoto Mitsunaga et al.   GUT

Priceless GEMMs: genetically engineered mouse models for colorectal cancer drug development

(2012) Jatin Roper et al.   TRENDS IN PHARMACOLOGICAL SCIENCES

Endogenous T Cell Responses to Antigens Expressed in Lung Adenocarcinomas Delay Malignant Tumor Progression

(2011) Michel DuPage et al.   CANCER CELL

Confocal laser endomicroscopy and narrow-band imaging-aided endoscopy for in vivo imaging of colitis and colon cancer in mice

(2011) Maximilian J Waldner et al.   Nature Protocols

In Vivo Fluorescence-Based Endoscopic Detection of Colon Dysplasia in the Mouse Using a Novel Peptide Probe

(2011) Sharon J. Miller et al.   PLoS One

The Dual PI3K/mTOR Inhibitor NVP-BEZ235 Induces Tumor Regression in a Genetically Engineered Mouse Model of PIK3CA Wild-Type Colorectal Cancer

(2011) Jatin Roper et al.   PLoS One

Utilization of Murine Colonoscopy for Orthotopic Implantation of Colorectal Cancer

(2011) Ehud Zigmond et al.   PLoS One

Generation of a Transgenic Mouse for Colorectal Cancer Research with Intestinal Cre Expression Limited to the Large Intestine

(2010) Y. Xue et al.   MOLECULAR CANCER RESEARCH

Paneth cells constitute the niche for Lgr5 stem cells in intestinal crypts

(2010) Toshiro Sato et al.   NATURE

Development of a mouse model for sporadic and metastatic colon tumors and its use in assessing drug treatment

(2010) K. E. Hung et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche

(2009) Toshiro Sato et al.   NATURE

Conditional mouse lung cancer models using adenoviral or lentiviral delivery of Cre recombinase

(2009) Michel DuPage et al.   Nature Protocols